Use of Advanced Mass Spectrometry to understand the form and function of a membrane protein complex
Integral membrane proteins are key components of cellular bioenergetics, transport and signal transduction processes. In the thylakoid membranes of cyanobacteria and chloroplasts, Photosystem II (PSII) is a large membrane-protein complex that undergoes an intricate, tightly regulated cycle of assembly, damage, and repair.
The available x-ray and cryo-EM structures of PSII are an essential foundation for understanding PSII function, but nonetheless provide only a snapshot of the active complex. To study aspects of the PSII life-cycle, advanced mass spectrometry (MS) has emerged as a powerful tool that can be used in conjunction with biochemical techniques.
In this presentation, I plan to present the MS-based approaches that are currently being used to study PSII composition, dynamics and structure, as well as post-translational modifications of amino acid residues. Such approaches are readily applicable to study other membrane bound molecular machines.
|Date||Friday, 8 December 2017|
|Time||12:00pm - 1:00pm|
|Event Category||Health Sciences|
|Location||Biochemistry Seminar Room 231|