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Department of Biochemistry profile

Dr Miriam Sharpe

PositionResearch Fellow
DepartmentDepartment of Biochemistry
QualificationsPhD
Research summaryProtein structure and function

Research

I am interested in production of light by the New Zealand glowworm, and the proteins that catalyse and control this bioluminescence. By investigating the structures and mechanisms of action of these proteins, I hope to develop new bioluminescent biotechnological tools.

My other interests include the characterisation of neuraminidases from drug-resistant influenza strains, the development of new inhibitors of influenza neuraminidases, and the structure and function of alanine racemases from pathogenic bacteria.

Publications

Gimenez, G., Metcalf, P., Paterson, N. G., & Sharpe, M. L. (2016). Mass spectrometry analysis and transcriptome sequencing reveal glowing squid crystal proteins are in the same superfamily as firefly luciferase. Scientific Reports, 6, 27638. doi: 10.1038/srep27638

Sharpe, M. L., Dearden, P. K., Gimenez, G., & Krause, K. L. (2015). Comparative RNA seq analysis of the New Zealand glowworm Arachnocampa luminosa reveals bioluminescence-related genes. BMC Genomics, 16, 825. doi: 10.1186/s12864-015-2006-2

Sharpe, M. L., Hastings, J. W., & Krause, K. L. (2014). Luciferases and light-emitting accessory proteins: Structural biology. In eLS. Chichester, UK: John Wiley & Sons. doi: 10.1002/9780470015902.a0003064.pub2

Margueron, R., Justin, N., Ohno, K., Sharpe, M. L., Son, J., Drury. III, W. J., … Gamblin, S. J. (2009). Role of the polycomb protein EED in the propagation of repressive histone marks. Nature, 431, 762-767. doi: 10.1038/nature08398

Im, H., Sharpe, M. L., Strych, U., Davlieva, M., & Krause, K. L. (2011). The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design. BMC Microbiology, 11, 116. doi: 10.1186/1471-2180-11-116

Chapter in Book - Research

Sharpe, M. L., Hastings, J. W., & Krause, K. L. (2014). Luciferases and light-emitting accessory proteins: Structural biology. In eLS. Chichester, UK: John Wiley & Sons. doi: 10.1002/9780470015902.a0003064.pub2

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Journal - Research Article

Gimenez, G., Metcalf, P., Paterson, N. G., & Sharpe, M. L. (2016). Mass spectrometry analysis and transcriptome sequencing reveal glowing squid crystal proteins are in the same superfamily as firefly luciferase. Scientific Reports, 6, 27638. doi: 10.1038/srep27638

Sharpe, M. L., Dearden, P. K., Gimenez, G., & Krause, K. L. (2015). Comparative RNA seq analysis of the New Zealand glowworm Arachnocampa luminosa reveals bioluminescence-related genes. BMC Genomics, 16, 825. doi: 10.1186/s12864-015-2006-2

Im, H., Sharpe, M. L., Strych, U., Davlieva, M., & Krause, K. L. (2011). The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design. BMC Microbiology, 11, 116. doi: 10.1186/1471-2180-11-116

Patrick, W. M., Nakatani, Y., Cutfield, S. M., Sharpe, M. L., Ramsay, R. J., & Cutfield, J. F. (2010). Carbohydrate binding sites in Candida albicans exo-β-1,3-glucanase and the role of the Phe-Phe 'clamp' at the active site entrance. FEBS Journal, 277, 4549-4561. doi: 10.1111/j.1742-4658.2010.07869.x

Margueron, R., Justin, N., Ohno, K., Sharpe, M. L., Son, J., Drury. III, W. J., … Gamblin, S. J. (2009). Role of the polycomb protein EED in the propagation of repressive histone marks. Nature, 431, 762-767. doi: 10.1038/nature08398

Sharpe, M. L., Gao, C., Kendall, S. L., Baker, E. N., & Lott, J. S. (2008). The structure and unusual protein chemistry of hypoxic response protein 1, a latency anitgen and highly expressed member of the DosR regulation in Mycobacterium tuberculosis. Journal of Molecular Biology, 383(4), 822-836. doi: 10.1016/j.jmb.2008.07.001

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