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Department of Biochemistry profile

Professor Kurt Krause

PositionProfessor
DepartmentDepartment of Biochemistry
QualificationsMA PhD MD
Research summaryStructural biology of infectious diseases

Research

Professor Krause is the Director of Webster Centre for Infectious Diseases.

His research areas include the structural biology of infectious diseases, structure-aided drug design, protein crystallography, X-ray diffraction, structure and function of enzymes and proteins—including antibiotic targets, redox proteins, nucleases, bioluminescence proteins—and bacterial pathogenesis factors.

Publications

Christensen, E. M., Patel, S. M., Korasick, D. A., Campbell, A. C., Krause, K. L., Becker, D. F., & Tanner, J. J. (2017). Resolving the cofactor binding site in the proline biosynthetic enzyme human pyrroline 5-carboxylate reductase 1. Journal of Biological Chemistry. Advance online publication. doi: 10.1074/jbc.M117.780288

Nakatani, Y., Opel-Reading, H. K., Merker, M., Machado, D., Andres, S., Kumar, S. S., … Aung, H. L., Cook, G. M., … Krause, K., & Köser, C. U. (2017). Role of alanine racemase mutations in Mycobacterium tuberculosis D-cycloserine resistance. Antimicrobial Agents & Chemotherapy. Advance online publication. doi: 10.1128/aac.01575-17

Mortuza, R., Aung, H. L., Taiaroa, G., Opel-Reading, H. K., Kleffmann, T., Cook, G. M., & Krause, K. L. (2017). Overexpression of a newly identified d-amino acid transaminase in Mycobacterium smegmatis complements glutamate racemase deletion. Molecular Microbiology. Advance online publication. doi: 10.1111/mmi.13877

Fagerlund, R. D., Wilkinson, M. E., Klykov, O., Barendregt, A., Pearce, F. G., Kieper, S. N., Maxwell, H. W. R., … Krause, K. L., Bostina, M., … Staals, R. H. J., & Fineran, P. C. (2017). Spacer capture and integration by a type I-F Cas1–Cas2-3 CRISPR adaptation complex. PNAS. Advance online publication. doi: 10.1073/pnas.1618421114

Wilkinson, M. E., Nakatani, Y., Staals, R. H. J., Kieper, S. N., Opel-Reading, H. K., McKenzie, R. E., Fineran, P. C., & Krause, K. L. (2016). Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system. Biochemistry Journal, 473, 1063-1072. doi: 10.1042/bcj20160078

Chapter in Book - Research

Sharpe, M. L., Hastings, J. W., & Krause, K. L. (2014). Luciferases and light-emitting accessory proteins: Structural biology. In eLS. Chichester, UK: John Wiley & Sons. doi: 10.1002/9780470015902.a0003064.pub2

Hastings, J. W., & Krause, K. L. (2006). Luciferases and light-emitting accessory proteins: Structural biology. In Encyclopedia of Life Sciences. New York: John Wiley & Sons. doi: 10.1038/npg.els.0003064

Krause, K. L., & Miller, M. D. (2002). A new engine for cleaving nucleic acid. In G. R. Eaton, D. C. Wiley & O. Jardetzky (Eds.), Structures and mechanisms: From ashes to enzymes. (pp. 270-293). Washington, DC, USA: American Chemical Society.

Krause, K. L., & Miller, M. D. (2001). Using electrostatics to define the active site of Serratia endonuclease. In C. H. Schein (Ed.), Methods in molecular biology, Volume 160: Nuclease methods and protocols. (pp. 249-262). Totowa, NJ, USA: Humana Press.

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Chapter in Book - Other

Hastings, J. W., & Krause, K. L. (2007). Luciferases and light-emitting accessory proteins: Structural biology. In Encyclopedia of Life Sciences. New York: John Wiley & Sons.

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Journal - Research Article

Christensen, E. M., Patel, S. M., Korasick, D. A., Campbell, A. C., Krause, K. L., Becker, D. F., & Tanner, J. J. (2017). Resolving the cofactor binding site in the proline biosynthetic enzyme human pyrroline 5-carboxylate reductase 1. Journal of Biological Chemistry. Advance online publication. doi: 10.1074/jbc.M117.780288

Fagerlund, R. D., Wilkinson, M. E., Klykov, O., Barendregt, A., Pearce, F. G., Kieper, S. N., Maxwell, H. W. R., … Krause, K. L., Bostina, M., … Staals, R. H. J., & Fineran, P. C. (2017). Spacer capture and integration by a type I-F Cas1–Cas2-3 CRISPR adaptation complex. PNAS. Advance online publication. doi: 10.1073/pnas.1618421114

Mortuza, R., Aung, H. L., Taiaroa, G., Opel-Reading, H. K., Kleffmann, T., Cook, G. M., & Krause, K. L. (2017). Overexpression of a newly identified d-amino acid transaminase in Mycobacterium smegmatis complements glutamate racemase deletion. Molecular Microbiology. Advance online publication. doi: 10.1111/mmi.13877

Nakatani, Y., Opel-Reading, H. K., Merker, M., Machado, D., Andres, S., Kumar, S. S., … Aung, H. L., Cook, G. M., … Krause, K., & Köser, C. U. (2017). Role of alanine racemase mutations in Mycobacterium tuberculosis D-cycloserine resistance. Antimicrobial Agents & Chemotherapy. Advance online publication. doi: 10.1128/aac.01575-17

Wilkinson, M. E., Nakatani, Y., Staals, R. H. J., Kieper, S. N., Opel-Reading, H. K., McKenzie, R. E., Fineran, P. C., & Krause, K. L. (2016). Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system. Biochemistry Journal, 473, 1063-1072. doi: 10.1042/bcj20160078

Sharif, S., Nakatani, Y., Wise, L., Corbett, M., Real, N. C., Stuart, G. S., Lateef, Z., Krause, K., Mercer, A. A., & Fleming, S. B. (2016). A broad-spectrum chemokine-binding protein of bovine papular stomatitis virus inhibits neutrophil and monocyte infiltration in inflammatory and wound models of mouse skin. PLoS ONE, 11(12), e0168007. doi: 10.1371/journal.pone.0168007

Sowerby, S. J., Crump, J. A., Johnstone, M. C., Krause, K. L., & Hill, P. C. (2016). Smartphone microscopy of parasite eggs accumulated into a single field of view. American Journal of Tropical Medicine & Hygiene, 94(1), 227-230. doi: 10.4269/ajtmh.15-0427

Poen, S., Nakatani, Y., Opel-Reading, H. K., Lassé, M., Dobson, R. C. J., & Krause, K. L. (2016). Exploring the structure of glutamate racemase from Mycobacterium tuberculosis as a template for anti-mycobacterial drug discovery. Biochemistry Journal, 473, 1267-1280. doi: 10.1042/bcj20160186

Couñago, R. M., Knapp, K. M., Nakatani, Y., Fleming, S. B., Corbett, M., Wise, L. M., Mercer, A. A., & Krause, K. L. (2015). Structures of orf virus chemokine binding protein in complex with host chemokines reveal clues to broad binding specificity. Structure, 23(7), 1199-1213. doi: 10.1016/j.str.2015.04.023

Sharpe, M. L., Dearden, P. K., Gimenez, G., & Krause, K. L. (2015). Comparative RNA seq analysis of the New Zealand glowworm Arachnocampa luminosa reveals bioluminescence-related genes. BMC Genomics, 16, 825. doi: 10.1186/s12864-015-2006-2

Davis, E., Scaletti-Hutchinson, E., Opel-Reading, H., Nakatani, Y., & Krause, K. L. (2014). The structure of alanine racemase from Acinetobacter baumannii. Acta Crystallographica Section F, 70(9), 1199-1205.

Li, Y., Mortuza, R., Milligan, D. L., Tran, S. L., Strych, U., Cook, G. M., & Krause, K. L. (2014). Investigation of the essentiality of glutamate racemase in Mycobacterium smegmatis. Journal of Bacteriology, 196(24), 4239-4244. doi: 10.1128/jb.02090-14

Baker, E. S., Luckner, S. R., Krause, K. L., Lambden, P. R., Clarke, I. N., & Ward, V. K. (2012). Inherent structural disorder and dimerisation of murine norovirus NS1-2 protein. PLoS ONE, 7(2), e30534. doi: 10.1371/journal.pone.0030534

Scaletti, E. R., Luckner, S. R., & Krause, K. L. (2012). Structural features and kinetic characterization of alanine racemase from Staphylococcus aureus (Mu50). Acta Crystallographica Section D, 68, 82-92. doi: 10.1107/S0907444911050682

Anthony, K. G., Strych, U., Yeung, K. R., Shoen, C. S., Perez, O., Krause, K. L., … Koski, R. A. (2011). New classes of alanine racemase inhibitors identified by high-throughput screening show antimicrobial activity against Mycobacterium tuberculosis. PLoS ONE, 6(5), e20374. doi: 10.1371/journal.pone.0020374

Im, H., Sharpe, M. L., Strych, U., Davlieva, M., & Krause, K. L. (2011). The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design. BMC Microbiology, 11, 116. doi: 10.1186/1471-2180-11-116

Couñago, R. M., Fleming, S. B., Mercer, A. A., & Krause, K. L. (2010). Crystallization and preliminary X-ray analysis of the chemokine-binding protein from orf virus (Poxviridae). Acta Crystallographica Section F, F66, 819-823. doi: 10.1107/S1744309110018166

Couñago, R. M., Davlieva, M., Strych, U., Hill, R. E., & Krause, K. L. (2009). Biochemical and structural characterization of alanine racemase from Bacillus anthracis (ames). BMC Structural Biology, 9, 53. doi: 10.1186/1472-6807-9-53

Chen, C., Krause, K., & Pettitt, B. M. (2009). Advantage of being a dimer for Serratia marcescens endonuclease. Journal of Physical Chemistry B, 113(2), 511-521. doi: 10.1021/jp8057838

Strych, U., Davlieva, M., Longtin, J. P., Murphy, E. L., Im, H., Benedik, M. J., & Krause, K. L. (2007). Purification and preliminary crystallization of alanine racemase from Streptococcus pneumoniae. BMC Microbiology, 7, 40. doi: 10.1186/1471-2180-7-40

Milligan, D. L., Tran, S. L., Strych, U., Cook, G. M., & Krause, K. L. (2007). The alanine racemase of Mycobacterium smegmatis is essential for growth in the absence of D-alanine. Journal of Bacteriology, 189(22), 8381-8386.

Weksberg, T. E., Lynch, G. C., Krause, K. L., & Pettitt, B. M. (2007). Molecular dynamics simulations of Trichomonas vaginalis ferredoxin show a loop-cap transition. Biophysical Journal, 92(10), 3337-3345.

Chen, C., Beck, B. W., Krause, K., Weksberg, T. E., & Pettitt, B. M. (2007). Effects of dimerization of Serratia marcescens endonuclease on water dynamics. Biopolymers, 85(3), 241-252.

Shelburne, S. A., Visnegarwala, F., Adams, C., Krause, K. L., Hamill, R. J., & White, A. C. (2005). Unusual manifestations of disseminated Histoplasmosis in patients responding to antiretroviral therapy. American Journal of Medicine, 118, 1038-1041.

LeMagueres, P., Im, H., Ebalunode, J., Strych, U., Benedik, M. J., Briggs, J. M., … Krause, K. L. (2005). The 1.9 Å crystal structure of alanine racemase from Mycobacterium tuberculosis contains a conserved entryway into the active site. Biochemistry, 44, 1471-1481.

Kim, M. G., Strych, U., Krause, K., Benedik, M., & Kohn, H. (2003). N(2)-substituted D,L-cycloserine derivatives: Synthesis and evaluation as alanine racemase inhibitors. Journal of Antibiotics, 56(2), 160-168.

LeMagueres, P., Im, H., Dvorak, A., Strych, U., Benedik, M., & Krause, K. L. (2003). Crystal structure at 1.45 Å resolution of alanine racemase from a pathogenic bacterium, Pseudomonas aeruginosa, contains both internal and external aldimine forms. Biochemistry, 42(50), 14752-14761.

Kim, M. G., Strych, U., Krause, K., Benedik, M., & Kohn, H. (2003). Evaluation of amino-substituted heterocyclic derivatives as alanine racemase inhibitors. Medicinal Chemistry Research, 12(3), 130-138.

Liu, L., Im, H., Cegielski, M., LeMagueres, P., Schultz, L. W., Krause, K. L., & Hastings, J. W. (2003). Characterization and crystallization of active domains of a novel luciferase from a marine dinoflagellate. Acta Crystallographica Section D, D59, 761-764.

Vidakovic, M., Crossnoe, C. R., Neidre, C., Kim, K., Krause, K. L., & Germanas, J. P. (2003). Reactivity of reduced [2Fe-2S] Ferredoxins parallels host susceptibility to nitroimidazoles. Antimicrobial Agents & Chemotherapy, 47(1), 302-308.

Musher, D. M., Dowell, M. E., Shortridge, V. D., Flamm, R. K., Jorgensen, J. H., Le Magueres, P., & Krause, K. L. (2002). Emergence of macrolide resistance during treatment of pneumococcal pneumonia. New England Journal of Medicine, 346(8), 630-631.

Crossnoe, C. R., Germanas, J. P., LeMagueres, P., Mustata, G., & Krause, K. L. (2002). The crystal structure of Trichomonas vaginalis ferredoxin provides insight into metronidazole activation. Journal of Molecular Biology, 318(2), 503-518.

Strych, U., Penland, R. L., Jimenez, M., Krause, K. L., & Benedik, M. J. (2001). Characterization of the alanine racemases from two Mycobacteria. FEMS Microbiology Letters, 196, 93-98.

Strych, U., Huang, H. C., Krause, K. L., & Benedik, M. J. (2000). Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1. Current Microbiology, 41, 290-294.

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Journal - Research Other

Chen, C., Beck, B. W., Krause, K., & Pettitt, B. M. (2006). Solvent participation in Serratia marcescens endonuclease complexes. Proteins, 62, 982-995.

Hastings, J. W., & Krause, K. L. (2005). Luciferase and light-emitting accessory proteins: Structural biology. Encyclopedia of Life Sciences, 1-10. John Wiley & Sons. Retrieved from http://els.wiley.com/els/subscriber/article/article_main.asp?sessionid=51f344ccd6954a92&srch=search&aid=A0003064

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