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GENE221 Molecular and Microbial Genetics

Mutations; genetic analysis in bacteria; mobile genetic elements; genetic analysis of regulatory circuits; DNA cloning and sequencing in genetic analysis; comparative microbial genomics; genetics and evolution of viruses of eukaryotes.

GENE 221 provides foundation material on molecular and microbial genetics. Topics include mutation, gene transfer and genetic analysis in bacteria, DNA cloning and comparative genomics of bacteria, fungi and the HIV retrovirus. Discoveries and methodologies in microbial genetics underlie all aspects of modern biology, and therefore, this paper is directly relevant for students with an interest in any of the biological sciences. The lecture course is complemented by a laboratory course that includes four experiments that together give hands-on experience of many of the genetic principles that are discussed in the lectures.

Paper title Molecular and Microbial Genetics
Paper code GENE221
Subject Genetics
EFTS 0.1500
Points 18 points
Teaching period First Semester
Domestic Tuition Fees (NZD) $1,018.05
International Tuition Fees (NZD) $4,500.00

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Prerequisite
CELS 191 and CHEM 191 and 36 further points
Restriction
GENE 211
Recommended Preparation or Concurrent Study
BIOC 221
Schedule C
Science
Eligibility
Essential for students majoring in genetics or microbiology and appropriate for students majoring in biochemistry, molecular biotechnology and all other biological sciences.
Contact
peter.fineran@otago.ac.nz
Teaching staff
Convenor: Associate Professor Peter Fineran (Department of Microbiology & Immunology)
Other teaching staff: Professor Iain Lamont (Department of Biochemistry), Professor Clive Ronson (Department of Microbiology & Immunology), Dr Gillian Mackay (Genetics Programme) and Associate Professor Russell Poulter (Department of Biochemistry)
Paper Structure
The lecture course is divided into 6 modules:
  • Mutations - the basis of genetics
  • Gene transfer and genetic analysis in bacteria
  • Genetic analysis of regulatory circuits
  • Comparative microbial genomics and evolution
  • Comparative fungal genomics
  • Genetics of HIV
The lecture course is complemented by a laboratory course, which provides training in relevant genetic methods and data analysis, including mutagen detection using the Ames test, gene mapping by conjugation, transposon mutagenesis and DNA cloning using a plasmid vector.
Teaching Arrangements
There are six weeks of laboratory classes, one every second week, and students are assigned to one of four lab streams (Monday and Friday afternoons; first lab on the first Friday of semester).
Textbooks
The recommended text book for GENE 221 is:
Introduction to Genetic Analysis (Griffiths, Wessler, Carroll, Doebley), 11th Edition. Earlier editions of this book are also satisfactory if you have access to a copy.
Course outline
View the information sheet for GENE 221
Graduate Attributes Emphasised
Global perspective, Interdisciplinary perspective, Scholarship, Critical thinking, Self-motivation, Teamwork.
View more information about Otago's graduate attributes.
Learning Outcomes
The broad objectives of GENE 221 are to understand:
  • The molecular basis and significance of mutation
  • Classical genetics of bacteria
  • The genetic approaches used in analysing gene regulation in bacteria
  • DNA cloning as a tool for genetic analysis
  • The use of comparative genomics in understanding the evolution of bacteria, yeast, other fungi and the HIV retrovirus

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Timetable

First Semester

Location
Dunedin
Teaching method
This paper is taught On Campus
Learning management system
Blackboard

Lecture

Stream Days Times Weeks
Attend
L1 Wednesday 12:00-12:50 9-15, 17-22
Thursday 10:00-10:50 9-15, 17-22
AND
M1 Monday 10:00-10:50 17-22

Practical

Stream Days Times Weeks
Attend one stream from
P1 Friday 14:00-17:50 9, 11, 13, 18, 20
Saturday 09:00-12:50 17
P2 Monday 14:00-17:50 10, 12, 14, 17, 19, 21
P3 Monday 14:00-17:50 11, 13, 15, 18, 20, 22
P4 Friday 14:00-17:50 10, 12, 14, 17, 19, 21
P5 Wednesday 14:00-17:50 10, 12, 14, 17-18, 20

Mutations; genetic analysis in bacteria; mobile genetic elements; genetic analysis of regulatory circuits; DNA cloning and sequencing in genetic analysis; comparative microbial genomics; genetics and evolution of viruses of eukaryotes.

GENE 221 provides foundation material on molecular and microbial genetics. Topics include mutation, gene transfer and genetic analysis in bacteria, DNA cloning and comparative genomics of bacteria, fungi and the HIV retrovirus. Discoveries and methodologies in microbial genetics underlie all aspects of modern biology, and therefore, this paper is directly relevant for students with an interest in any of the biological sciences. The lecture course is complemented by a laboratory course that includes four experiments that together give hands-on experience of many of the genetic principles that are discussed in the lectures.

Paper title Molecular and Microbial Genetics
Paper code GENE221
Subject Genetics
EFTS 0.1500
Points 18 points
Teaching period First Semester
Domestic Tuition Fees Tuition Fees for 2018 have not yet been set
International Tuition Fees Tuition Fees for international students are elsewhere on this website.

^ Top of page

Prerequisite
CELS 191 and CHEM 191 and 36 further points
Restriction
GENE 211
Recommended Preparation or Concurrent Study
BIOC 221
Schedule C
Science
Eligibility
Essential for students majoring in genetics or microbiology and appropriate for students majoring in biochemistry, molecular biotechnology and all other biological sciences.
Contact
peter.fineran@otago.ac.nz
Teaching staff
Convenor: Associate Professor Peter Fineran (Department of Microbiology & Immunology)
Other teaching staff: Professor Iain Lamont (Department of Biochemistry), Professor Clive Ronson (Department of Microbiology & Immunology), Dr Gillian Mackay (Genetics Programme) and Associate Professor Russell Poulter (Department of Biochemistry)
Paper Structure
The lecture course is divided into 6 modules:
  • Mutations - the basis of genetics
  • Gene transfer and genetic analysis in bacteria
  • Genetic analysis of regulatory circuits
  • Comparative microbial genomics and evolution
  • Comparative fungal genomics
  • Genetics of HIV
The lecture course is complemented by a laboratory course, which provides training in relevant genetic methods and data analysis, including mutagen detection using the Ames test, gene mapping by conjugation, transposon mutagenesis and DNA cloning using a plasmid vector.
Teaching Arrangements
There are six weeks of laboratory classes, one every second week, and students are assigned to one of four lab streams (Monday and Friday afternoons; first lab on the first Friday of semester).
Textbooks
The recommended text book for GENE 221 is:
Introduction to Genetic Analysis (Griffiths, Wessler, Carroll, Doebley), 11th Edition. Earlier editions of this book are also satisfactory if you have access to a copy.
Course outline
View the information sheet for GENE 221
Graduate Attributes Emphasised
Global perspective, Interdisciplinary perspective, Scholarship, Critical thinking, Self-motivation, Teamwork.
View more information about Otago's graduate attributes.
Learning Outcomes
The broad objectives of GENE 221 are to understand:
  • The molecular basis and significance of mutation
  • Classical genetics of bacteria
  • The genetic approaches used in analysing gene regulation in bacteria
  • DNA cloning as a tool for genetic analysis
  • The use of comparative genomics in understanding the evolution of bacteria, yeast, other fungi and the HIV retrovirus

^ Top of page

Timetable

First Semester

Location
Dunedin
Teaching method
This paper is taught On Campus
Learning management system
Blackboard

Lecture

Stream Days Times Weeks
Attend
L1 Wednesday 12:00-12:50 9-13, 15-16, 18-22
Thursday 10:00-10:50 9-13, 15-22
AND
M1 Monday 10:00-10:50 17-22

Practical

Stream Days Times Weeks
Attend one stream from
P1 Friday 14:00-17:50 9, 11, 16, 18, 20
Saturday 10:00-13:50 15
P2 Monday 14:00-17:50 10, 12, 15, 17, 19, 21
P3 Wednesday 14:00-17:50 10, 12, 15, 17, 19, 21
P4 Friday 14:00-17:50 10, 12, 15, 17, 19, 21
P5 Monday 14:00-17:50 11, 13, 16, 18, 20, 22