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Dr Craig Marshall

Senior Lecturer, Department of Biochemistry

Dr Craig MarshallCraig's research is focused on cold adaptations; specifically, organisms that live in cold places. Most of the world's organisms tolerate freeze-thaw cycles—some freeze, others super cool; all have strategies to cope with sub-zero temperatures.

In particular, Craig's work centres on:

  • What pathways are important in cold tolerance?
  • How do proteins protect against freezing or ice damage?
  • How do enzymes function at low temperatures?
  • What other factors cause stress at low temperatures?

Craig's work also involves identifying proteins important in ice formation, and in identifying genes that are associated with freezing and thawing in an Antarctic nematode, Panagrolaimus davidi.

Graig Marshall's profile on the Department of Biochemistry website

Teaching

Craig teaches in GENE 411 Current Topics in Genetics.

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Publications

Thorne, M. A. S., Seybold, A., Marshall, C., & Wharton, D. (2017). Molecular snapshot of an intracellular freezing event in an Antarctic nematode. Cryobiology, 75, 117-124. doi: 10.1016/j.cryobiol.2017.01.003

Papetti, C., Windisch, H. S., La Mesa, M., Lucassen, M., Marshall, C., & Lamare, M. D. (2016). Non-Antarctic notothenioids: Past phylogenetic history and contemporary phylogeographic implications in the face of environmental changes. Marine Genomics, 25. doi: 10.1016/j.margen.2015.11.007

Marshall, C. J., Basu, K., & Davies, P. L. (2016). Ice-shell purification of ice-binding proteins. Cryobiology, 72(3), 258-263. doi: 10.1016/j.cryobiol.2016.03.009

Papetti, C., Windisch, H., La Mesa, M., Lucassen, M., Marshall, C., & Lamare, M. (2016). Non-Antarctic notothenioids: Past phylogenetic history and contemporary phylogeographic implications in the face of environmental changes. Proceedings of the Scientific Committee on Antarctic Research (SCAR) XXXIV Conference: Antarctic in the Global Earth System: From the Poles to the Tropics. (pp. 759).

Kasparova, E., Van de Putte, A. P., Marshall, C., & Janko, K. (2015). Lifestyle and ice: The relationship between ecological specialization and response to Pleistocene climate change. PLoS ONE, 10(11), e0138766. doi: 10.1371/journal.pone.0138766

Journal - Research Article

Thorne, M. A. S., Seybold, A., Marshall, C., & Wharton, D. (2017). Molecular snapshot of an intracellular freezing event in an Antarctic nematode. Cryobiology, 75, 117-124. doi: 10.1016/j.cryobiol.2017.01.003

Papetti, C., Windisch, H. S., La Mesa, M., Lucassen, M., Marshall, C., & Lamare, M. D. (2016). Non-Antarctic notothenioids: Past phylogenetic history and contemporary phylogeographic implications in the face of environmental changes. Marine Genomics, 25. doi: 10.1016/j.margen.2015.11.007

Marshall, C. J., Basu, K., & Davies, P. L. (2016). Ice-shell purification of ice-binding proteins. Cryobiology, 72(3), 258-263. doi: 10.1016/j.cryobiol.2016.03.009

Kasparova, E., Van de Putte, A. P., Marshall, C., & Janko, K. (2015). Lifestyle and ice: The relationship between ecological specialization and response to Pleistocene climate change. PLoS ONE, 10(11), e0138766. doi: 10.1371/journal.pone.0138766

Raymond, M. R., Wharton, D. A., & Marshall, C. J. (2014). Nematodes from the Victoria Land coast, Antarctica and comparisons with cultured Panagrolaimus davidi. Antarctic Science, 26(1), 15-22. doi: 10.1017/S0954102013000230

Thorne, M. A. S., Kagoshima, H., Clark, M. S., Marshall, C. J., & Wharton, D. A. (2014). Molecular analysis of the cold tolerant Antarctic nematode, Panagrolaimus davidi. PLoS ONE, 9(8), e104526. doi: 10.1371/journal.pone.0104526

MacKenzie, A. L., & Marshall, C. (2014). Polysaccharidases in the crystalline styles of selectively bred greenshell mussel (Perna canaliculus Gmelin) families. Journal of Shellfish Research, 33(3), 687-694. doi: 10.2983/035.033.0303

MacKenzie, A. L., & Marshall, C. (2014). Proteins in the crystalline styles of the marine mussels Perna Canaliculus Gmelin and Mytilus galloprovincialis Lamarck. Journal of Shellfish Research, 33(3), 673-685. doi: 10.2983/035.033.0302

Hawes, T. C., Marshall, C. J., & Wharton, D. A. (2014). A 9kDa antifreeze protein from the Antarctic springtail, Gomphiocephalus hodgsoni. Cryobiology, 69(1), 181-183. doi: 10.1016/j.cryobiol.2014.07.001

Raymond, M. R., Wharton, D. A., & Marshall, C. J. (2013). Factors determining nematode distributions at Cape Hallett and Gondwana station, Antarctica. Antarctic Science, 25(3), 347-357. doi: 10.1017/S0954102012001162

MacKenzie, L. A., Selwood, A. I., & Marshall, C. (2012). Isolation and characterization of an enzyme from the Greenshell™ mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid. Toxicon, 60(3), 406-419. doi: 10.1016/j.toxicon.2012.05.005

Van de Putte, A. P., Janko, K., Kasparova, E., Maes, G. E., Rock, J., Koubbi, P., … Marshall, C. (2012). Comparative phylogeography of three trematomid fishes reveals contrasting genetic structure patterns in benthic and pelagic species. Marine Genomics, 8, 23-34. doi: 10.1016/j.margen.2012.05.002

Hawes, T. C., Marshall, C. J., & Wharton, D. A. (2012). Ultraviolet radiation tolerance of the Antarctic springtail, Gomphiocephalus hodgsoni. Antarctic Science, 24(2), 147-153. doi: 10.1017/S0954102011000812

Janko, K., Marshall, C., Musilová, Z., Van Houdt, J., Couloux, A., Cruaud, C., & Lecointre, G. (2011). Multilocus analyses of an Antarctic fish species flock (Teleostei, Notothenioidei, Trematominae): Phylogenetic approach and test of the early-radiation event. Molecular Phylogenetics & Evolution, 60(3), 305-316. doi: 10.1016/j.ympev.2011.03.008

Hawes, T. C., Marshall, C. J., & Wharton, D. A. (2011). Antifreeze proteins in the Antarctic springtail, Gressittacantha terranova. Journal of Comparative Physiology B, 181(6), 713-719. doi: 10.1007/s00360-011-0564-4

Wharton, D. A., Selvanesan, L., & Marshall, C. J. (2010). Ice-active proteins from New Zealand snow tussocks, Chionochloa macra and c. Rigida. CryoLetters, 31(3), 239-248.

Wharton, D. A., & Marshall, C. J. (2009). How do terrestrial Antarctic organisms survive in their harsh environment? Journal of Biology, 8, 39. doi: 10.1186/jbiol142

Isely, N., Lamare, M., Marshall, C., & Barker, M. (2009). Expression of the DNA repair enzyme, photolyase, in developmental tissues and larvae, and in response to ambient UV-R in the Antarctic sea urchin Sterechinus neumayeri. Photochemistry & Photobiology, 85(5), 1168-1176. doi: 10.1111/j.1751-1097.2009.00566.x

Wharton, D. A., Pow, B., Kristensen, M., Ramløv, H., & Marshall, C. J. (2009). Ice-active proteins and cryoprotectants from the New Zealand alpine cockroach, Celatoblatta quinquemaculata. Journal of Insect Physiology, 55(1), 27-31. doi: 10.1016/j.jinsphys.2008.09.007

Smith, T., Wharton, D. A., & Marshall, C. J. (2008). Cold tolerance of an Antarctic nematode that survives intracellular freezing: Comparisons with other nematode species. Journal of Comparative Physiology B, 178(1), 93-100. doi: 10.1007/s00360-007-0202-3

Kumble, K. D., Demmer, J., Fish, S., Hall, C., Corrales, S., DeAth, A., … Luxmanan, S., Marshall, C. J., & Wharton, D. A. (2008). Characterization of a family of ice-active proteins from the Ryegrass, Lolium perenne. Cryobiology, 57(3), 263-268. doi: 10.1016/j.cryobiol.2008.09.005

Janko, K., Lecointre, G., DeVries, A., Couloux, A., Cruaud, C., & Marshall, C. (2007). Did glacial advances during the Pleistocene influence differently the demographic histories of benthic and pelagic Antarctic shelf fishes? Inferences from intraspecific mitochondrial and nuclear DNA sequence diversity. BMC Evolutionary Biology, 7, 220. doi: 10.1186/1471-2148-7-220

Wharton, D. A., Wilson, P. W., Mutch, J. S., Marshall, C. J., & Lim, M. (2007). Recrystallization inhibition assessed by splat cooling and optical recrystallometry. CryoLetters, 28(1), 61-68.

Lamare, M. D., Barker, M. F., Lesser, M. P., & Marshall, C. (2006). DNA photorepair in echinoid embryos: Effects of temperature on repair rate in Antarctic and non-Antarctic species. Journal of Experimental Biology, 209, 5017-5028. doi: 10.1242/jeb.02598

Wharton, D. A., Barrett, J., Goodall, G., Marshall, C. J., & Ramløv, H. (2005). Ice-active proteins from the Antarctic nematode Panagrolaimus davidi. Cryobiology, 51, 198-207.

Wharton, D. A., Downes, M. F., Goodall, G., & Marshall, C. J. (2005). Freezing and cryoprotective dehydration in an Antarctic nematode (Panagrolaimus davidi) visualised using a freeze substitution technique. Cryobiology, 50, 21-28.

Wharton, D. A., Mutch, J. S., Wilson, P. W., Marshall, C. J., & Lim, M. (2004). A simple ice nucleation spectrometer. CryoLetters, 25, 335-340.

Wharton, D. A., Goodall, G., & Marshall, C. J. (2003). Freezing survival and cryoprotective dehydration as cold tolerance mechanisms in the Antarctic nematode Panagrolaimus davidi. Journal of Experimental Biology, 206, 215-221.

Wharton, D. A., Goodall, G., & Marshall, C. J. (2002). Freezing rate affects the survival of a short-term freezing stress in Panagrolaimus davidi, an Antarctic nematode that survives intracellular freezing. CryoLetters, 23, 5-10.

Denton, M. J., Marshall, C. J., & Legge, M. (2002). The protein folds as platonic forms: New support for the pre-darwinian conception of evolution by natural law. Journal of Theoretical Biology, 219(3), 325-342.

Sharpe, M., Love, C., & Marshall, C. (2001). Lactate dehydrogenase from the Antarctic eelpout, Lycodichthys dearborni. Polar Biology, 24(4), 258-269. doi: 10.1007/s003000000206

Sharp, M., Love, C., & Marshall, C. J. (2001). Lactate dehydrogenase from the Antarctic eelpout Lycodichthys dearborni. Polar Biology, 24, 258-269.

Denton, M. J., & Marshall, C. J. (2001). Laws of form revisted. Concepts, 410, 417.

Denton, M. J., & Marshall, C. J. (2001). Laws of form revisited. Nature, 410, 417.

Piotte, C. P., Hunter, A. K., Marshall, C. J., & Grigor, M. R. (1998). Phylogenetic analysis of three lipocalin-like proteins present in the milk of Trichosurus vulpecula (Phalangeridae, Marsupialia). Journal of Molecular Evolution, 46, 361-369.

Piotte, C. P., Marshall, C. J., Hubbard, M. J., Collet, C., & Grigor, M. R. (1997). Lysozyme and α-lactalbumin from the milk of a marsupial, the common brush-tailed possum (Trichosurus vulpecula). Biochimica et Biophysica Acta: General Subjects, 1336(2), 235-242. doi: 10.1016/S0304-4165(97)00033-0

Marshall, C. J. (1997). Cold-adapted enzymes. Trends in Biotechnology, 15(9), 359-364.

Cutfield, S. M., Dodson, E. J., Anderson, B. F., Marshall, C. J., Moody, P. C., Sullivan, P. A., & Cutfield, J. F. (1995). The crystal structure of a major secreted aspartic proteinase from Candida albicans in complexes with two inhibitors. Structure, 3, 1261-1271.

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Conference Contribution - Published proceedings: Abstract

Papetti, C., Windisch, H., La Mesa, M., Lucassen, M., Marshall, C., & Lamare, M. (2016). Non-Antarctic notothenioids: Past phylogenetic history and contemporary phylogeographic implications in the face of environmental changes. Proceedings of the Scientific Committee on Antarctic Research (SCAR) XXXIV Conference: Antarctic in the Global Earth System: From the Poles to the Tropics. (pp. 759).

Kumar, A., Knapp, K., Krause, K., & Marshall, C. (2012). Revisiting the structure of lactate dehydrogenase from Spiny Dogfish. Protein Science, 21(S1), (pp. 95). doi: 10.1002/pro.2113

Raymond, M., Marshall, C., & Wharton, D. (2012). Living on the edge: Nematodes in Victoria Land, Antarctica. Proceedings of the Polar Environment Research Theme (PERT) Workshop. Retrieved from http://polarresearch.otago.ac.nz/pert-workshop-2012/

Liddy, M., Lamare, M., Marshall, C., & Isely, N. (2009). Expression of the DNA repair enzyme, photolyase, in embryos of the Antarctic sea urchin, Sterechinus Neumayeri, in response to increases in UV-R during ozone depletion. Proceedings of the Annual Antarctic Conference. (pp. 100). Christchurch, New Zealand: Antarctica New Zealand.

Clarke, S. R., Marshall, C. J., & Wharton, D. A. (2009). Evidence for a new mechanism for ice active proteins. Proceedings of the ComBio2009 Conference. [CD-ROM]

Sharma, M., Vulin, C., Clarke, S., & Marshall, C. J. (2009). Characterisation of an antifreeze protein from the meal worm, Tenebrio molitor. Proceedings of the ComBio2009 Conference. [CD-ROM]

Raymond, M. R., Marshall, C. J., & Wharton, D. A. (2008). Defining habitats for Antarctic nematode species from the Ross Sea region. Proceedings of the Annual Antarctic Conference. (pp. 53). Christchurch, New Zealand: Antarctica New Zealand.

Isely, N., Marshall, C., & Lamare, M. (2008). The enzyme photolyase is induced in echinoderms by exposure to UV light. Proceedings of the Annual Antarctic Conference. (pp. 55). Christchurch, New Zealand: Antarctica New Zealand.

Wharton, D. A., Raymond, M. R., Clarke, S. R., & Marshall, C. J. (2008). An Antarctic nematode that survives intracellular freezing. Proceedings of the Annual Antarctic Conference. (pp. 17). Christchurch, New Zealand: Antarctica New Zealand.

Wharton, D. A., Mutch, J. S., Wilson, P. W., Marshall, C. J., & Lim, M. (2003). A simple ice nucleation spectrometer. Proceedings of the International Symposium on Animal and Plant Cold Hardiness. Czech Republic: TEMP.

Goodall, G., Wharton, D. A., Barrett, J., Ramlov, H., & Marshall, C. J. (2001). Ice active compounds and intracellular freezing in an Antarctic nematode (Panagrolaimus davidi). VIII International Biology Symposium, Amsterdam. Scientific Committee for Antarctic Research.

Marshall, C. J., Crossman, D., Love, D. R., McInnes, S. J., & Fleming, R. I. (2000). Structural studies of cold-adaptation: Lactate dehydrogenases from notothenioid fish. Antarctic Ecosystems: Models for Wider Ecological Understanding. (pp. 123-133). Christchurch: NZ Sciences.

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