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Health Sciences profile

Professor Gregory Cook

PositionProfessor
DepartmentDepartment of Microbiology and Immunology
QualificationsMSc PhD(Waik) FRSNZ
Research summaryCellular and molecular bacterial physiology

Research

My current research is aimed at gaining mechanistic insight into how bacteria sense and respond to their environment at the level of the cytoplasmic membrane. The membrane proteins and signaling pathways involved and how they control the physiological response of a particular bacterium to environmental signals is a major focus.

Current research projects:

  • Adaptation of the membrane-bound F1Fo ATP synthase to function under different environments
  • Energetics of Mycobacterium tuberculosis: adaptation to hypoxia and low energy conditions
  • Bacterial sensing of antimicrobial stress

Publications

Greening, C., Berney, M., Hards, K., Cook, G. M., & Conrad, R. (2014). A soil actinobacterium scavenges atmospheric H2 using two membrane-associated, oxygen-dependent [NiFe] hydrogenases. PNAS, 111(11), 4257-4261. doi: 10.1073/pnas.1320586111

Berney, M., Greening, C., Conrad, R., Jacobs, Jr, W. R., & Cook, G. M. (2014). An obligately aerobic soil bacterium activates fermentative hydrogen production to survive reductive stress during hypoxia. PNAS, 111(31), 11479-11484. doi: 10.1073/pnas.1407034111

Heikal, A., Nakatani, Y., Dunn, E., Weimar, M. R., Day, C. L., Baker, E. N., … Cook, G. M. (2014). Structure of the bacterial type II NADH dehydrogenase: A monotopic membrane protein with an essential role in energy generation. Molecular Microbiology, 91(5), 950-964. doi: 10.1111/mmi.12507

Hards, K., Robson, J. R., Berney, M., Shaw, L., Bald, D., Koul, A., … Cook, G. M. (2015). Bactericidal mode of action of bedaquiline. Journal of Antimicrobial Chemotherapy, 70, 2028-2037. doi: 10.1093/jac/dkv054

Berney, M., Greening, C., Hards, K., Collins, D., & Cook, G. M. (2014). Three different [NiFe] hydrogenases confer metabolic flexibility in the obligate aerobe Mycobacterium smegmatis. Environmental Microbiology, 16(1), 318-330. doi: 10.1111/1462-2920.12320

Chapter in Book - Research

Cook, G. M., Greening, C., Hards, K., & Berney, M. (2014). Energetics of pathogenic bacteria and opportunities for drug development. In Advances in microbial physiology. Elsevier. doi: 10.1016/bs.ampbs.2014.08.001

Cook, G. M., Berney, M., Gebhard, S., Heinemann, M., Cox, R. A., Danilchanka, O., & Niederweis, M. (2009). Physiology of mycobacteria. In R. K. Poole (Ed.), Advances in microbial physiology (Vol. 55). (pp. 83-183). London: Elsevier. doi: 10.1016/s0065-2911(09)05502-7

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Journal - Research Article

Hards, K., Robson, J. R., Berney, M., Shaw, L., Bald, D., Koul, A., … Cook, G. M. (2015). Bactericidal mode of action of bedaquiline. Journal of Antimicrobial Chemotherapy, 70, 2028-2037. doi: 10.1093/jac/dkv054

Berney, M., Greening, C., Hards, K., Collins, D., & Cook, G. M. (2014). Three different [NiFe] hydrogenases confer metabolic flexibility in the obligate aerobe Mycobacterium smegmatis. Environmental Microbiology, 16(1), 318-330. doi: 10.1111/1462-2920.12320

Greening, C., Berney, M., Hards, K., Cook, G. M., & Conrad, R. (2014). A soil actinobacterium scavenges atmospheric H2 using two membrane-associated, oxygen-dependent [NiFe] hydrogenases. PNAS, 111(11), 4257-4261. doi: 10.1073/pnas.1320586111

Hartman, T., Weinrick, B., Vilchèze, C., Berney, M., Tufariello, J., Cook, G. M., & Jacobs, Jr, W. R. (2014). Succinate dehydrogenase is the regulator of respiration in Mycobacterium tuberculosis. PLoS Pathogens, 10(11), e1004510. doi: 10.1371/journal.ppat.1004510

Berney, M., Greening, C., Conrad, R., Jacobs, Jr, W. R., & Cook, G. M. (2014). An obligately aerobic soil bacterium activates fermentative hydrogen production to survive reductive stress during hypoxia. PNAS, 111(31), 11479-11484. doi: 10.1073/pnas.1407034111

Dunn, E. A., Roxburgh, M., Larsen, L., Smith, R. A. J., McLellan, A. D., Heikal, A., Murphy, M. P., & Cook, G. M. (2014). Incorporation of triphenylphosphonium functionality improves the inhibitory properties of phenothiazine derivatives in Mycobacterium tuberculosis. Bioorganic & Medicinal Chemistry, 22(19), 5320-5328. doi: 10.1016/j.bmc.2014.07.050

Pecsi, I., Hards, K., Ekanayaka, N., Berney, M., Hartman, T., Jacobs, Jr, W. R., & Cook, G. M. (2014). Essentiality of succinate dehydrogenase in Mycobacterium smegmatis and its role in the generation of the membrane potential under hypoxia. mBio, 5(4), e01093-14. doi: 10.1128/mBio.01093-14

Cook, G. M., Hards, K., Vilchèze, C., Hartman, T., & Berney, M. (2014). Energetics of respiration and oxidative phosphorylation in mycobacteria. Microbiology Spectrum, 2(3), MGM2-0015-2013. doi: 10.1128/microbiolspec.MGM2-0015-2013

Heikal, A., Nakatani, Y., Dunn, E., Weimar, M. R., Day, C. L., Baker, E. N., … Cook, G. M. (2014). Structure of the bacterial type II NADH dehydrogenase: A monotopic membrane protein with an essential role in energy generation. Molecular Microbiology, 91(5), 950-964. doi: 10.1111/mmi.12507

Lagautriere, T., Bashiri, G., Paterson, N. G., Berney, M., Cook, G. M., & Baker, E. N. (2014). Characterization of the proline-utilization pathway in Mycobacterium tuberculosis through structural and functional studies. Acta Crystallographica Section D, 70(Pt 4), 968-980. doi: 10.1107/s1399004713034391

Kingston, A. W., Zhao, H., Cook, G. M., & Helmann, J. D. (2014). Accumulation of heptaprenyl diphosphate sensitizes Bacillus subtilis to bacitracin: Implications for the mechanism of resistance mediated by the BceAB transporter. Molecular Microbiology, 93(1), 37-49. doi: 10.1111/mmi.12637

Schulz, S., Iglesias-Cans, M., Krah, A., Yildiz, Ö., Leone, V., Matthies, D., Cook, G. M., … Meier, T. (2013). A new type of Na+-driven ATP synthase membrane rotor with a two-carboxylate ion-coupling motif. PLoS Biology, 11(6), e1001596. doi: 10.1371/journal.pbio.1001596

Wilson, J. L., Jesse, H. E., Hughes, B., Lund, V., Naylor, K., Davidge, K. S., Cook, G. M., … Poole, R. K. (2013). Ru(CO)3Cl(glycinate) (CORM-3): A carbon monoxide–releasing molecule with broad-spectrum antimicrobial and photosensitive activities against respiration and cation transport in Escherichia coli. Antioxidants & Redox Signaling, 19(5), 497-509. doi: 10.1089/ars.2012.4784

Frampton, R., Aggio, R. B. M., Villas-Bôas, S. G., Arcus, V. L., & Cook, G. M. (2012). Toxin-antitoxin systems of Mycobacterium smegmatis are essential for cell survival. Journal of Biological Chemistry, 287(8), 5340-5356. doi: 10.1074/jbc.M111.286856

Tannock, G. W., Wilson, C. M., Loach, D., Cook, G. M., Eason, J., O'Toole, P. W., … Lawley, B. (2012). Resource partitioning in relation to cohabitation of Lactobacillus species in the mouse forestomach. ISME Journal, 6, 927-938. doi: 10.1038/ismej.2011.161

Berney, M., Weimar, M. R., Heikal, A., & Cook, G. M. (2012). Regulation of proline metabolism in mycobacteria and its role in carbon metabolism under hypoxia. Molecular Microbiology, 84(4), 664-681. doi: 10.1111/j.1365-2958.2012.08053.x

Taniguchi, N., Suzuki, T., Berney, M., Yoshida, M., & Cook, G. M. (2011). The regulatory C-terminal domain of subunit ε of FoF1 ATP synthase is dispensable for growth and survival of Escherichia coli. Journal of Bacteriology, 193(8), 2046-2052. doi: 10.1128/JB.01422-10

Sims, I. M., Frese, S. A., Walter, J., Loach, D., Wilson, M., Appleyard, K., … Livingston, M., Baird, M., Cook, G., & Tannock, G. W. (2011). Structure and functions of exopolysaccharide produced by gut commensal Lactobacillus reuteri 100-23. ISME Journal, 5(7), 1115-1124. doi: 10.1038/ismej.2010.201

Yu, Z., Bekker, M., Tramonti, A., Cook, G. M., van Ulsen, P., Scheffers, D.-J., … Luirink, J. (2011). Activators of glutamate-dependent acid resistance system alleviate deleterious effects of YidC depletion in Escherichia coli. Journal of Bacteriology, 193(6), 1308-1316. doi: 10.1128/JB.01209-10

Arcus, V. L., McKenzie, J. L., Robson, J., & Cook, G. M. (2011). The PIN-domain ribonucleases and the prokaryotic VapBC toxin—antitoxin array. Protein Engineering, Design & Selection, 24(1-2), 33-40. doi: 10.1093/protein/gzq081

Kalamorz, F., Keis, S., McMillan, D. G. G., Olsson, K., Stanton, J.-A., Stockwell, P., Black, M. A., … Cook, G. M. (2011). Draft genome sequence of the thermoalkaliphilic Caldalkalibacillus thermarum strain TA2.A1. Journal of Bacteriology, 193(16), 4290-4291. doi: 10.1128/jb.05035-11

Swe, P. M., Heng, N. C. K., Cook, G. M., Tagg, J. R., & Jack, R. W. (2010). Identification of Dysl, the immunity factor of the streptococcal bacteriocin dysgalacticin. Applied & Environmental Microbiology, 76(23), 7885-7889. doi: 10.1128/AEM.01707-10

Berney, M., & Cook, G. M. (2010). Unique flexibility in energy metabolism allows mycobacteria to combat starvation and hypoxia. PLoS ONE, 5(1), e8614. doi: 10.1371/journal.pone.0008614

McMillan, D. G. G., Velasquez, I., Nunn, B. L., Goodlett, D. R., Hunter, K. A., Lamont, I., Sander, S. G., & Cook, G. M. (2010). Acquisition of iron by alkaliphilic Bacillus species. Applied & Environmental Microbiology, 76(20), 6955-6961. doi: 10.1128/AEM.01393-10

Hümpel, A., Gebhard, S., Cook, G. M., & Berney, M. (2010). The SigF regulon in Mycobacterium smegmatis reveals roles in adaptation to stationary phase, heat and oxidative stress. Journal of Bacteriology, 192(10), 2491-2502. doi: 10.1128/JB.00035-10

Shepherd, M., Sanguinetti, G., Cook, G. M., & Poole, R. K. (2010). Compensations for diminished terminal oxidase activity in Escherichia coli: Cytochrome bd-II-mediated respiration and glutamate metabolism. Journal of Biological Chemistry, 285(24), 18464-18472. doi: 10.1074/jbc.M110.118448

Swe, P. M., Cook, G. M., Tagg, J. R., & Jack, R. W. (2009). Mode of action of dysgalacticin: A large heat-labile bacteriocin. Journal of Antimicrobial Chemotherapy, 63(4), 679-686. doi: 10.1093/jac/dkn552

Gebhard, S., Gaballa, A., Helmann, J. D., & Cook, G. M. (2009). Direct stimulus perception and transcription activation by a membrane-bound DNA binding protein. Molecular Microbiology, 73(3), 482-491. doi: 10.1111/j.1365-2958.2009.06787.x

Matthies, D., Preiss, L., Klyszejko, A. L., Muller, D. J., Cook, G. M., Vonck, J., & Meier, T. (2009). The C13 ring from a thermoalkaliphilic ATP synthase reveals an extended diameter due to a special structural region. Journal of Molecular Biology, 388(3), 611-618. doi: 10.1016/j.jmb.2009.03.052

Robson, J., McKenzie, J. L., Cursons, R., Cook, G. M., & Arcus, V. L. (2009). The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-antitoxin module that controls growth via inhibition of translation. Journal of Molecular Biology, 390, 353-367. doi: 10.1016/j.jmb.2009.05.006

McMillan, D. G. G., Keis, S., Berney, M., & Cook, G. M. (2009). Nonfermentative thermoalkaliphilic growth is restricted to alkaline environments. Applied & Environmental Microbiology, 75(24), 7649-7654. doi: 10.1128/AEM.01639-09

Mesbah, N. M., Cook, G. M., & Wiegel, J. (2009). The halophilic alkalithermophile Natranaerobius thermophilus adapts to multiple environmental extremes using a large repertoire of Na+(K+)/H+ antiporters. Molecular Microbiology, 74(2), 270-281. doi: 10.1111/j.1365-2958.2009.06845.x

Gauntlett, J. C., Gebhard, S., Keis, S., Manson, J. M., Pos, K. M., & Cook, G. M. (2008). Molecular analysis of BcrR, a membrane-bound bacitracin sensor and DNA-binding protein from Enterococcus faecalis. Journal of Biological Chemistry, 283(13), 8591-8600. doi: 10.1074/jbc.M709503200

Meier, T., Morgner, N., Matthies, D., Pogoryelov, D., Keis, S., Cook, G. M., … Brutschy, B. (2007). A tridecameric c ring of the adenosine triphosphate (ATP) synthase from the thermoalkaliphilic Bacillus sp. strain TA2.A1 facilitates ATP synthesis at low electrochemical proton potential. Molecular Microbiology, 65(5), 1181-1192.

McMillan, D. G. G., Keis, S., Dimroth, P., & Cook, G. M. (2007). A specific adaptation in the a subunit of thermoalkaliphilic F1Fo-ATP synthase enables ATP synthesis at high pH but not at neutral pH values. Journal of Biological Chemistry, 282(24), 17395-17404.

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Journal - Research Other

Cook, G. M., & Heikal, A. (2012). Bridging the gap between a TB drug and its target. Science Translational Medicine, 4(150), 150fs33. doi: 10.1126/scitranslmed.3004816

McMillan, D. G. G., Ferguson, S. A., Dey, D., Schröder, K., Aung, H. L., Carbone, V., … Cook, G. M. (2011). A1Ao-ATP synthase of Methanobrevibacter ruminantium couples sodium ions for ATP synthesis under physiological conditions. Journal of Biological Chemistry, 286(46), 39882-39892. doi: 10.1074/jbc.M111.281675

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