Expertise
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Ábraham lab techniques list
Dr Andrea Kwakowsky (Post-doc):
Very experienced in: immunohisto- and cytochemistry, in situ hybridization, light and confocal microscopy, calcium-imaging, breeding and genotyping of transgenic mice, transgenic technology, genomic PCR, RT-PCR, Western blotting
Some experience in: cell culture techniques, pharmacology, small animal surgery
Emeline Tolod Kemp (Assistant Research Fellow):
Very experienced in: Immunohistochemistry, immunocytochemistry, immunofluorescence, tissue and blood collection, animal handling and surgeries, light and confocal Microscopy, PCR, Real-time PCR, Western Blot, in situ hybridization, autoradiography, ELISA, protein assays, cell culture (primary cell lines and human embryonic stem cells)
Zsuzsanna Barad (Assistant Research Fellow):
Very experienced in: immunoprecipitation, PAGE, WB, ELISA, ELISPOT, RNA isolation, RT-PCR, animal handling (GDX, ip/sc injections), cell isolation from mouse immunological tissues, cell sorting, flow cytometry, immunohistochemistry, recombinant protein production and purification
Daniil Potapov (Research Assistant):
Very experienced in: Microtome sectioning, cryosectioning, immunohistochemistry, histology, light microscopy, fluorescence and confocal microscopy, PCR genotyping, rat and mouse breeding, small animals stereotaxic surgery and handling, optical imaging in vivo
Some experience in: Plasmid cloning, in situ hybridization, RNA amplification, mouse-tail tip blood-sampling, electrophysiology (intra- and extracellular single cell recording)
Anderson lab techniques list
Rachael Augustine (Assistant Research Fellow):
Very experienced in: immunohistochemistry, small animal surgery (rat and mouse), stereotaxic surgery, qPCR, in situ hybridization, RIAs, rodent transcardiac perfusions, brain sectioning, iodinations, laser microdissection, monitoring estrus cycles (rodent), microdissection of hypothalamic tissue, molecular biology techniques
Some experience in: cloning, genotyping, mouse breeding and handling, microscopy
Chris Kemp (Assistant Research Fellow):
Very experienced in: Immunohistochemistry, rodent transcardiac perfusions, light/fluorescence microscopy, RNA isolation, real-time PCR, genotyping, ELISAs
Some experience in: RIA, Western blotting, confocal microscopy, laser capture microdissection, stereotaxic and ovariectomy surgery, rodent pre-ovulatory estrogen surge induction models, mouse breeding and handling
Janette Quennell (Post-doc):
Very experienced in: qPCR, in situ hybridisation, laser microdissection, light microscopy, genotyping, mouse breeding, mouse surgery and handling, LH surge, Estrogen -ve feedback protocols, general molecular biology techniques, cloning
Some experience in: RNA amplification, single-cell PCR, immunocytochemistry, RIA, confocal microscopy, stereotaxic surgery
Brown lab techniques list
Nural Abdul (Research Fellow):
Very experienced in: Immunohistochemistry, animal handling, microtome and cyrostat sectioning, i.c.v. and jugular cannulation, stereotaxic surgery
Some experience in: PCR
Victoria Scott (Post-doc):
Very experienced in: Rat in vivo electrophysiology, rat stereotaxic surgery, rat jugular vein and femoral vein cannulation
Some experience in: Immunohistochemistry, microtome and cryostat sectioning, in situ hybridisation, sharp electrode and patch clamp electrophysiology, molecular biology
Bunn lab techniques list
Shirley Douglas (Assistant Research Fellow):
Very experienced in: culture of primary chromaffin cells and measurement of noradrenaline accumulation and release and tyrosine hydroxylase activity; protein measurement and Western Blotting of chromaffin cell and mouse brain punch samples; fluorescent immunocytochemistry
Limited experience in: culture of cell lines; plasmid preparation; transfecting cells; making glycerol stocks; extraction of RNA by RNeasy kit; RT-PCR, qPCR
Campbell lab techniques list
Mel Prescott (Assistant Research Fellow):
Some experience in: animal handling, breeding & blood sampling, histology, immunocytochemistry, microtome and cyrosectioning, light and confocal microscopy
Grattan lab techniques list
Chris Booker (Assistant Research Fellow):
Very experienced in: general molecular biology techniques such as PCR, systematic review methodology
Some experience in: in situ hybridisation, stereotaxic surgery, fixed-interval/extinction rodent behavioural training, BBC Basic Skinner box programming, multiple regression analysis, evidence-based medicine evaluation, epidemiology & public health interventions
Pene Knowles (Research Technician):
Some experience in: immunocytochemistry, in situ hybridization, light and confocal microscopy, genotyping, Western blotting, cell culture techniques, tissue and blood collection, animal handling, protein assays, RIA, rodent transcardiac perfusions, brain sectioning
Ilona Kokay (Research Fellow):
Very experienced in: in situ hybridisation, immunohistochemistry, HPLC, molecular biology techniques including qPCR, small animal surgery (e.g. ovariectomy), monitoring estrus cycles (rodent), microdissection of hypothalamic tissue, radioligand binding and autoradiography
Some experience in: microdialysis, ribonuclease protection assays, RIAs, stereotaxic surgery
Sharon Ladyman (Research Fellow):
Very experienced in: small animal surgery, blood sampling, animal monitoring (eg feeding) immunohistochemistry, molecular biology techniques including qPCR, monitoring estrus cycles (rodent), microdissection of hypothalamic tissue, Western blotting
Some experience in: behavioral work (eg maternal), ELISA, RIA
Caroline Larsen (Post-doc):
Very experienced in: behavioral work, immunohistochemistry, rodent handling and surgeries, brain sectioning, microscopy, genotyping, RIA
Some experience in: molecular biology techniques including RT-PCR
Herbison lab techniques list
Jenny Clarkson (Post-doc):
Very experienced in: Immunocytochemisty, small animal surgery, confocal microscopy, monitoring estrus cycles, monitoring puberty
Some experience in: RIA, RT-PCR, electrophysiology
Stephanie Constantin (Post-doc):
Very experienced in: Calcium imaging, Electrophysiology, RNA work (extraction, RT-PCR - tissue or single cell), DNA work (extraction/genotyping, PCR - tissue or single cell), Primary cell culture, Immunocytochemistry (culture, sections from cryostat or vibratome)
Some experience in: In situ hybridization (riboprobes or oligos), RIA
XinHuai Lui (Research Fellow):
Very experienced in: electrophysiology (patch clamping) in vitro, RIA and ICC
Some experience in: western blot and confocal microscopy
Rob Porteous (Research Assistant):
Very experienced in: Immunohistochemistry, microtome sectioning, RIA, Ovx/GDX surgeries, mouse-tail tip blood sampling, injection protocols, general Herbilab genotyping (primers available for most of the transgenic lines we use). LH surge, Estrogen -ve feedback protocols
Some experience in: Mouse jugular cannulation, photomicrography, histology, paraffin sectioning, cryosectioning
Juan Roa Rivas (Post-doc):
Very experienced in: RNA and DNA isolation, RT-PCR, Real-time PCR, Plasmid cloning, in situ hybridization, Western blotting, animal handling and surgeries (GDX, ip/sc/icv injections, jugular blood-sampling), RIA, cryosectioning, light microscopy
Some experience in: calcium-imaging, enzyme assays, HPLC, IHC
Jasoni lab techniques list
Chew Ling Tan (Research Assistant):
Experienced in: culture of primary embryonic neurons and cell lines, immunohistochemistry, confocal microscopy, mouse colony maintenance and timed breeding
Some experience in: in situ hybridisation, DNA cloning