fPCR (Faecal PCR) is a diagnostic test which allows the direct quantitation of the shedding of Map bacteria in faeces as a means of assessing severity of Johne’s disease. The quantitative result allows a distinction to be quickly made between high, medium and low or background shedders and facilitates the prompt identification, stratification and selective culling of those most seriously affected animals as part of a Johne’s management plan.
This test is not species specific and can be applied to any submitted dung samples.
Samples should be booked in by phone or email prior to sending. Booked-in samples will be given priority.
Tel: 64 3 4894832
Tests cost $50+GST each. Samples submitted following preliminary herd screening by serology (Paralisa) are eligible for a discount, as are multiples of 10 samples – please enquire for more information.
Three to five grams (an amount approximately equivalent to one golf-ball) of cleanly pottled dung is requested for faecal PCR testing. Pottles should be labeled, clearly identifying the animal the sample has come from. Care should be taken to prevent cross-contamination of samples in transit (please do not submit samples inside examination gloves).
Please ensure that your samples are accompanied by a completed laboratory booking form (PDF 168 KB).
Please read our terms and conditions prior to sending your samples.
Expected turnaround time for results is 5–7 working days. Generally, samples received by Tuesday will be processed the same week with results being sent the following week. If results are urgent, please let the laboratory know at the time of booking.
DRL undertakes to perform all tests to the standard approved by the CVO but makes no claim to the tests being 100% accurate.
fPCR Results will be reported as Not Detected, Background, Low, Moderate, High and Very High (Supershedders).
Results will be provided to the vet involved, if indicated by the farmer. No other agency will receive the data.
Rory O’Brien, Alan Hughes, Simon Liggett and Frank Griffin: Composite testing for ante-mortem diagnosis of Johne’s disease in farmed New Zealand deer: correlations between bacteriological culture, histopathology, serological reactivity and faecal shedding as determined by quantitative PCR :BMC Veterinary Research 2013, 9:72
O'Brien R., Hughes A., Griffin F.: Quantitative PCR as an ancillary, anti-mortem diagnostic test for Johne's Disease in deer: A comparison between bacteriological culture, serological reactivity and faecal shedding Proceedings of the Deer Branch of the New Zealand Veterinary Association, Proceedings of the Deer Branch of the New Zealand Veterinary Association 28, pp 121-127, Jun 2011.
O'Brien R., Hughes A., Griffin F. Quantitative PCR as an ancillary, ante-mortem diagnostic test for Johne's disease in deer. Proceedings of the Deer Branch of the New Zealand Veterinary Association, Proceedings of the Deer Branch of the New Zealand Veterinary Association - Volume 27, pp 45-52, Jan 2010.