Dr Laura Gumy
| Position | Senior Lecturer |
|---|---|
| Department | Department of Anatomy |
| Research summary | Imaging; neurobiology |
Research
The main focus of my work is the microtubule cytoskeleton and microtubule-based vesicle transport in healthy and diseased/injured neurons. My research relies on combining high-resolution live cell imaging and quantitative analysis of cytoskeletal and transport dynamics, using advance microscopic assays and tools to visualise and manipulate proteins within cells. I also use different biochemical methods to characterise protein-protein interactions (pull-down assays and mass spectrometry-based protein identification). I employ primary neuronal cultures or non-neuronal cells to image live-tissue.
Publications
Mayo-Muñoz, D., Smith, L. M., Garcia-Doval, C., Malone, L. M., Harding, K. R., Jackson, S. A., Hampton, H. G., Fagerlund, R. D., Gumy, L. F., & Fineran, P. C. (2022). Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection. Molecular Cell. Advance online publication. doi: 10.1016/j.molcel.2022.10.028
Malone, L. M., Warring, S. L., Jackson, S. A., Warnecke, C., Gardner, P. P., Gumy, L. F., & Fineran, P. C. (2020). A jumbo phage that forms a nucleus-like structure evades CRISPR-Cas DNA targeting but is vulnerable to type III RNA-based immunity. Nature Microbiology, 5, 48-55. doi: 10.1038/s41564-019-0612-5
Gumy, L. F., & Hoogenraad, C. C. (2018). Local mechanisms regulating selective cargo entry and long-range trafficking in axons. Current Opinion in Neurobiology, 51, 23-28. doi: 10.1016/j.conb.2018.02.007
Abil, Z., Gumy, L. F., Zhao, H., & Hoogenraad, C. C. (2017). Inducible control of mRNA transport using reprogrammable RNA-binding proteins. ACS Synthetic Biology, 6(6), 950-956. doi: 10.1021/acssynbio.7b00025
Gumy, L. F., Katrukha, E. A., Grigoriev, I., Jaarsma, D., Kapitein, L. C., Akhmanova, A., & Hoogenraad, C. C. (2017). MAP2 defines a pre-axonal filtering zone to regulate KIF1-versus KIF5-dependent cargo transport in sensory neurons. Neuron, 94(2), 347-362. doi: 10.1016/j.neuron.2017.03.046
Mayo-Muñoz, D., Smith, L. M., Garcia-Doval, C., Malone, L. M., Harding, K. R., Jackson, S. A., Hampton, H. G., Fagerlund, R. D., Gumy, L. F., & Fineran, P. C. (2022). Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection. Molecular Cell. Advance online publication. doi: 10.1016/j.molcel.2022.10.028
Journal - Research Article
Malone, L. M., Warring, S. L., Jackson, S. A., Warnecke, C., Gardner, P. P., Gumy, L. F., & Fineran, P. C. (2020). A jumbo phage that forms a nucleus-like structure evades CRISPR-Cas DNA targeting but is vulnerable to type III RNA-based immunity. Nature Microbiology, 5, 48-55. doi: 10.1038/s41564-019-0612-5
Journal - Research Article
Gumy, L. F., & Hoogenraad, C. C. (2018). Local mechanisms regulating selective cargo entry and long-range trafficking in axons. Current Opinion in Neurobiology, 51, 23-28. doi: 10.1016/j.conb.2018.02.007
Journal - Research Article
Abil, Z., Gumy, L. F., Zhao, H., & Hoogenraad, C. C. (2017). Inducible control of mRNA transport using reprogrammable RNA-binding proteins. ACS Synthetic Biology, 6(6), 950-956. doi: 10.1021/acssynbio.7b00025
Journal - Research Article
Gumy, L. F., Katrukha, E. A., Grigoriev, I., Jaarsma, D., Kapitein, L. C., Akhmanova, A., & Hoogenraad, C. C. (2017). MAP2 defines a pre-axonal filtering zone to regulate KIF1-versus KIF5-dependent cargo transport in sensory neurons. Neuron, 94(2), 347-362. doi: 10.1016/j.neuron.2017.03.046
Journal - Research Article
Ruane, P. T., Gumy, L. F., Bola, B., Anderson, B., Wozniak, M. J., Hoogenraad, C. C., & Allan, V. J. (2016). Tumour suppressor adenomatous polyposis coli (APC) localisation is regulated by both kinesin-1 and kinesin-2. Scientific Reports, 6, 27456 . doi: 10.1038/srep27456
Journal - Research Article
Gumy, L. F., Katrukha, E. A., Kapitein, L. C., & Hoogenraad, C. C. (2014). New insights into mRNA trafficking in axons. Developmental Neurobiology, 74(3), 233-244. doi: 10.1002/dneu.22121
Journal - Research Article
Schlager, M. A., Serra-Marques, A., Grigoriev, I., Gumy, L. F., Esteves da Silva, M., Wulf, P. S., … Hoogenraad, C. C. (2014). Bicaudal D family adaptor proteins control the velocity of dynein-based movements. Cell Reports, 8(5), 1248-1256. doi: 10.1016/j.celrep.2014.07.052
Journal - Research Article
Gumy, L. F., Chew, D. J., Tortosa, E., Katrukha, E. A., Kapitein, L. C., Tolkovsky, A. M., … Fawcett, J. W. (2013). The kinesin-2 family member KIF3C regulates microtubule dynamics and is required for axon growth and regeneration. Journal of Neuroscience, 33(28), 11329-11345. doi: 10.1523/JNEUROSCI.5221-12.2013
Journal - Research Article
van der Vaart, B., van Riel, W. E., Doodhi, H., Kevenaar, J. T., Katrukha, E. A., Gumy, L., … Akhmanova, A. (2013). CFEOM1-associated kinesin KIF21A is a cortical microtubule growth inhibitor. Developmental Cell, 27(2), 145-160. doi: 10.1016/j.devcel.2013.09.010
Journal - Research Article
van Spronsen, M., van Battum, E. Y., Kuijpers, M., Vangoor, V. R., Rietman, M. L., Pothof, J., Gumy, L. F., … Hoogenraad, C. C. (2013). Developmental and activity-dependent miRNA expression profiling in primary hippocampal neuron cultures. PLoS ONE, 8(10), e74907. doi: 10.1371/journal.pone.0074907
Journal - Research Article
Tan, C. L., Andrews, M. R., Kwok, J. C. F., Heintz, T. G. P., Gumy, L. F., Fässler, R., & Fawcett, J. W. (2012). Kindlin-1 enhances axon growth on inhibitory chondroitin sulfate proteoglycans and promotes sensory axon regeneration. Journal of Neuroscience, 32(21), 7325-7335. doi: 10.1523/JNEUROSCI.5472-11.2012
Journal - Research Article
Gumy, L. F., & Hoogenraad, C. C. (2013). Off the rails: Axonal cargoes on the road to nowhere. EMBO Journal, 32(10). [Review].
Journal - Research Other
