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Biochemistry Special Seminar | Professor Himadri Pakrasi Washington University, Louis, MO, USA

Use of Advanced Mass Spectrometry to understand the form and function of a membrane protein complex

Integral membrane proteins are key components of cellular bioenergetics, transport and signal transduction processes. In the thylakoid membranes of cyanobacteria and chloroplasts, Photosystem II (PSII) is a large membrane-­protein complex that undergoes an intricate, tightly regulated cycle of assembly, damage, and repair. The available x-­ray and cryo-­EM structures of PSII are an essential foundation for understanding PSII function, but nonetheless provide only a snapshot of the active complex. To study aspects of the PSII life-­cycle, advanced mass spectrometry (MS) has emerged as a powerful tool that can be used in conjunction with biochemical techniques. In this presentation, I plan to present the MS-­based approaches that are currently being used to study PSII composition, dynamics and structure, as well as post-­‐translational modifications of amino acid residues. Such approaches are readily applicable to study other membrane bound molecular machines.

Date Friday, 8 December 2017
Time 12:00pm - 1:00pm
Audience Public
Event Category Health Sciences
Event Type Departmental Seminar
LocationBiochemistry seminar rm 231

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