Pseudouridine in small RNAs
RNA is made up of four key nucleotides, cytosine, guanine, uridine and adenosine. Pseudouridine (psi) is an isomer of uridine and is the most abundant modified nucleotide in RNA, garnering it the nickname "the 5th nucleoside".
Historically, psi was thought to be only present in structural RNAs such as tRNA, rRNA and snRNA; however, new techniques have allowed sensitive identification of psi in a genomewide manner.
We hypothesised that psi would be present in small RNAs from Arabidopsis thaliana, given their similarities to tRNAs in terms of processing and nuclear export.
To test this hypothesis we developed methods to detect psi in small RNAs in high- and low-throughput manners. Using these techniques on inflorescence and pollen tissue, we were able to detect psi in small RNAs, particularly a unique class of pollen small RNA called epigenetically-activated siRNA or "easiRNA".
The function of psi in easiRNA is currently unknown, although a number of hypotheses are being investigated.
|Date||Tuesday, 20 March 2018|
|Time||12:00pm - 1:00pm|
|Event Category||Health Sciences|
|Location||Biochemistry seminar rm 231|