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Biochemistry Seminar | Rowan Herridge

Pseudouridine in small RNAs

RNA is made up of four key nucleotides, cytosine, guanine, uridine and adenosine. Pseudouridine (psi) is an isomer of uridine and is the most abundant modified nucleotide in RNA, garnering it the nickname "the 5th nucleoside".

Historically, psi was thought to be only present in structural RNAs such as tRNA, rRNA and snRNA; however, new techniques have allowed sensitive identification of psi in a genome­wide manner.

We hypothesised that psi would be present in small RNAs from Arabidopsis thaliana, given their similarities to tRNAs in terms of processing and nuclear export.

To test this hypothesis we developed methods to detect psi in small RNAs in high-­ and low-throughput manners. Using these techniques on inflorescence and pollen tissue, we were able to detect psi in small RNAs, particularly a unique class of pollen small RNA called epigenetically-activated siRNA or "easiRNA".

The function of psi in easiRNA is currently unknown, although a number of hypotheses are being investigated.

Date Tuesday, 20 March 2018
Time 12:00pm - 1:00pm
Event Category Health Sciences
Event Type Departmental Seminar
LocationBiochemistry seminar rm 231

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