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Special Biochemistry seminar: Lung-Yu Liang, Walter and Eliza Hall Institute of Medical Research, Australia

EphB6 pseudokinase mediates a novel mode of cell-cell communication

EphB6 is a member of the Eph receptor tyrosine kinase (RTK) protein family. Kinase-active members of the Eph receptor family have dominated studies of this protein family to date. How EphB6 can control cell signalling has remained mysterious, along with the precise function of the pseudokinase domain – a domain within the intracellular portion of the receptor that lacks catalytic activity. It has remained unclear whether, EphB6 could bind to trans-presented ephrin ligands and whether such engagement could promote assembly of higher order signalling complexes. In this seminar, I will briefly discuss our recent published work on the biochemical and biophysical characterisation of the intracellular regions of EphB6. I will then discuss how we used an integrative approach to investigate the functions of full-length EphB6 in a cellular context. By employing a co-culture system and cutting-edge lattice light-sheet microscopy, we identified ephrinB1 as a ligand of EphB6. Binding of ephrinB1 to EphB6 led to co-clustering of EphB6/ephrinB1 at the plasma membrane. Strikingly, the EphB6/ephrinB1 clusters appear to promote formation of tubular structures connecting EphB6 and ephrinB1 expressing cells. To obtain details at a nanometer resolution, we established a correlative light and electron microscopy (CLEM) workflow, and performed Cryo-electron tomography (Cryo-ET) on these tubular structures. We discovered that these tubular structures were represented as an unprecedented double-membrane organisation. Our findings indicate that, although lacking kinase activity, the EphB6 pseudokinase can function by mediating a novel mode of cell-cell communication.

Zoom

Zoom link for Lung-Yu Liang's Biochemistry seminar

Zoom password: 175560

Date Monday, 29 November 2021
Time 12:00pm - 1:00pm
Audience Undergraduate students,Postgraduate students,Staff
Event Category Health Sciences
Event Type Seminar
Online
DepartmentBiochemistry
LocationOnline
CostFree
Contact NameDepartment of Biochemistry
Contact Phone+64 3 479 7863
Contact Emailbiochemistry@otago.ac.nz

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