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Department of Biochemistry profile

Dr Adam Middleton

PositionResearch Fellow
DepartmentDepartment of Biochemistry
QualificationsPhD
Research summaryProtein Biochemistry, Structural Biology, Apoptosis

Research

Adam works in Professor Catherine Day’s laboratory

Publications

Budhidarmo, R., Zhu, J., Middleton, A. J., & Day, C. L. (2018). The RING domain of RING Finger 11 (RNF11) protein binds Ubc13 and inhibits formation of polyubiquitin chains. FEBS Letters. Advance online publication. doi: 10.1002/1873-3468.13029

Middleton, A. J., Budhidarmo, R., Das, A., Zhu, J., Foglizzo, M., Mace, P. D., & Day, C. L. (2017). The activity of TRAF RING homo- and heterodimers is regulated by zinc finger 1. Nature Communications, 8, 1788. doi: 10.1038/s41467-017-01665-3

Groves, M. R., Schroer, C. F. E., Middleton, A. J., Lunev, S., Danda, N., Ali, A. M., … Williams, C. (2017). Structural insights into K48-linked ubiquitin chain formation by the Pex4p-Pex22p complex. Biochemical & Biophysical Research Communications. Advance online publication. doi: 10.1016/j.bbrc.2017.12.150

Middleton, A. J., Wright, J. D., & Day, C. L. (2017). Regulation of E2s: A role for additional ubiquitin binding sites? Journal of Molecular Biology, 429(22), 3430-3440. doi: 10.1016/j.jmb.2017.06.008

Foglizzo, M., Middleton, A. J., & Day, C. L. (2016). Structure and function of the RING domains of RNF20 and RNF40, dimeric E3 ligases that monoubiquitylate histone H2B. Journal of Molecular Biology, 428(20), 4073-4086. doi: 10.1016/j.jmb.2016.07.025

Chapter in Book - Research

Middleton, A. J., Budhidarmo, R., & Day, C. L. (2014). Use of E2~ubiquitin conjugates for the characterization of ubiquitin transfer by RING E3 ligases such as the inhibitor of apoptosis proteins. In A. Ashkenazi, J. A. Wells & J. Yuan (Eds.), Methods in enzymology (Vol. 545). (pp. 243-263). San Diego, CA: Elsevier. doi: 10.1016/B978-0-12-801430-1.00010-X

Middleton, A. J., Vanderbeld, B., Bredow, M., Tomalty, H., Davies, P. L., & Walker, V. K. (2014). Isolation and characterization of ice-binding proteins from higher plants. In D. K. Hincha & E. Zuther (Eds.), Plant cold acclimation: Methods and Protocols (Methods in Molecular Biology: Vol. 1166). (pp. 255-277). New York: Springer. doi: 10.1007/978-1-4939-0844-8_19

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Journal - Research Article

Budhidarmo, R., Zhu, J., Middleton, A. J., & Day, C. L. (2018). The RING domain of RING Finger 11 (RNF11) protein binds Ubc13 and inhibits formation of polyubiquitin chains. FEBS Letters. Advance online publication. doi: 10.1002/1873-3468.13029

Middleton, A. J., Budhidarmo, R., Das, A., Zhu, J., Foglizzo, M., Mace, P. D., & Day, C. L. (2017). The activity of TRAF RING homo- and heterodimers is regulated by zinc finger 1. Nature Communications, 8, 1788. doi: 10.1038/s41467-017-01665-3

Middleton, A. J., Wright, J. D., & Day, C. L. (2017). Regulation of E2s: A role for additional ubiquitin binding sites? Journal of Molecular Biology, 429(22), 3430-3440. doi: 10.1016/j.jmb.2017.06.008

Groves, M. R., Schroer, C. F. E., Middleton, A. J., Lunev, S., Danda, N., Ali, A. M., … Williams, C. (2017). Structural insights into K48-linked ubiquitin chain formation by the Pex4p-Pex22p complex. Biochemical & Biophysical Research Communications. Advance online publication. doi: 10.1016/j.bbrc.2017.12.150

Foglizzo, M., Middleton, A. J., & Day, C. L. (2016). Structure and function of the RING domains of RNF20 and RNF40, dimeric E3 ligases that monoubiquitylate histone H2B. Journal of Molecular Biology, 428(20), 4073-4086. doi: 10.1016/j.jmb.2016.07.025

Middleton, A. J., & Day, C. L. (2015). The molecular basis of lysine 48 ubiquitin chain synthesis by Ube2K. Scientific Reports, 5, 16793. doi: 10.1038/srep16793

Middleton, A. J., Marshall, C. B., Faucher, F., Bar-Dolev, M., Braslavsky, I., Campbell, R. L., … Davies, P. L. (2012). Antifreeze protein from freeze-tolerant grass has a beta-roll fold with an irregularly structured ice-binding site. Journal of Molecular Biology, 416, 713-724. doi: 10.1016/j.jmb.2012.01.032

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