Dr Torsten Kleffmann
Position | Senior Research Fellow |
---|---|
Department | Department of Biochemistry |
Qualifications | MSc PhD |
Research summary | Mass spectrometry-based proteomics and protein analysis |
Research
My research interests are mostly in mass spectrometry-based protein analysis and proteome research on various biological systems. At present my main function is the management of the Centre for Protein Research.
Therefore my major focus is on method establishment and method development rather than on individual research projects.
However I am involved in various research projects in the field of mass spectrometry-based proteome/protein research and new collaborations are emerging at present which will result in more definable projects and research interests in the future.
Publications
Philem, P., Kleffmann, T., Gai, S., Hawkins, B. C., Wilbanks, S. M., & Lamont, I. L. (2021). Identification of active site residues of the siderophore synthesis enzyme PvdF and evidence for interaction of PvdF with a substrate-providing enzyme. International Journal of Molecular Sciences, 22(4), 2211. doi: 10.3390/ijms22042211
Lassé, M., Pilbrow, A. P., Kleffmann, T., Andersson Överström, E., von Zychlinski, A., Frampton, C. M. A., … Troughton, R. W., Lewis, L. K., Prickett, T. C. R., Pemberton, C. J., Richards, A. M., & Cameron, V. A. (2021). Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals. Scientific Reports, 11(1), 4605. doi: 10.1038/s41598-021-84046-7
Sweetman, E., Kleffmann, T., Edgar, C., de Lange, M., Vallings, R., & Tate, W. (2020). A SWATH-MS analysis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome peripheral blood mononuclear cell proteomes reveals mitochondrial dysfunction. Journal of Translational Medicine, 18, 365. doi: 10.1186/s12967-020-02533-3
Handley, T. N. G., Tan, H.-X., Rutledge, M. T., Brewitz, H. H., Tyndall, J. D. A., Kleffmann, T., Butler, M. I., Poulter, R. T. M., & Wilbanks, S. M. (2020). Engineered biosynthesis of cyclotides. New Zealand Journal of Botany. Advance online publication. doi: 10.1080/0028825X.2020.1791914
Johnson, S. L., Borziak, K., Kleffmann, T., Rosengrave, P., Dorus, S., & Gemmell, N. J. (2020). Ovarian fluid proteome variation associates with sperm swimming speed in an externally fertilising fish. Journal of Evolutionary Biology. Advance online publication. doi: 10.1111/jeb.13717
Journal - Research Article
Philem, P., Kleffmann, T., Gai, S., Hawkins, B. C., Wilbanks, S. M., & Lamont, I. L. (2021). Identification of active site residues of the siderophore synthesis enzyme PvdF and evidence for interaction of PvdF with a substrate-providing enzyme. International Journal of Molecular Sciences, 22(4), 2211. doi: 10.3390/ijms22042211
Lassé, M., Pilbrow, A. P., Kleffmann, T., Andersson Överström, E., von Zychlinski, A., Frampton, C. M. A., … Troughton, R. W., Lewis, L. K., Prickett, T. C. R., Pemberton, C. J., Richards, A. M., & Cameron, V. A. (2021). Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals. Scientific Reports, 11(1), 4605. doi: 10.1038/s41598-021-84046-7
Sweetman, E., Kleffmann, T., Edgar, C., de Lange, M., Vallings, R., & Tate, W. (2020). A SWATH-MS analysis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome peripheral blood mononuclear cell proteomes reveals mitochondrial dysfunction. Journal of Translational Medicine, 18, 365. doi: 10.1186/s12967-020-02533-3
Johnson, S. L., Borziak, K., Kleffmann, T., Rosengrave, P., Dorus, S., & Gemmell, N. J. (2020). Ovarian fluid proteome variation associates with sperm swimming speed in an externally fertilising fish. Journal of Evolutionary Biology. Advance online publication. doi: 10.1111/jeb.13717
Handley, T. N. G., Tan, H.-X., Rutledge, M. T., Brewitz, H. H., Tyndall, J. D. A., Kleffmann, T., Butler, M. I., Poulter, R. T. M., & Wilbanks, S. M. (2020). Engineered biosynthesis of cyclotides. New Zealand Journal of Botany. Advance online publication. doi: 10.1080/0028825X.2020.1791914
Mehta, S., McKinney, C., Algie, M., Verma, C. S., Kannan, S., Harfoot, R., … Gould, M. L., Parker, K., … Cunliffe, H. E., … Kleffmann, T., Braithwaite, A. W., & Woolley, A. G. (2020). Dephosphorylation of YB-1 is required for nuclear localisation during G2 phase of the cell cycle. Cancers, 12(2), 315. doi: 10.3390/cancers12020315
Mehta, S., Algie, M., Al-Jabry, T., McKinney, C., Kannan, S., Verma, C. S., … Parker, K., Henderson, L., Gould, M. L., Bhatia, P., Harfoot, R., … Kleffmann, T., … Woolley, A. G., … Braithwaite, A. (2020). Critical role for cold shock protein YB-1 in cytokinesis. Cancers, 12, 2473. doi: 10.3390/cancers12092473
Murphy, E. L., Singh, K. V., Avila, B., Kleffmann, T., Gregory, S. T., Murray, B. E., Krause, K. L., … Jogl, G. (2020). Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis. Scientific Reports, 10, 16301. doi: 10.1038/s41598-020-73199-6
Crake, R. L. I., Phillips, E., Kleffmann, T., & Currie, M. J. (2019). Co-culture with human breast adipocytes differentially regulates protein abundance in breast cancer cells. Cancer Geonomics & Proteomics, 16(5), 319-332. doi: 10.21873/cgp.20137
Herbison, R., Evans, S., Doherty, J.-F., Algie, M., Kleffmann, T., & Poulin, R. (2019). A molecular war: Convergent and ontogenetic evidence for adaptive host manipulation in related parasites infecting divergent hosts. Proceedings of the Royal Society B, 286(1915), 20191827. doi: 10.1098/rspb.2019.1827
Mortuza, R., Aung, H. L., Taiaroa, G., Opel-Reading, H. K., Kleffmann, T., Cook, G. M., & Krause, K. L. (2018). Overexpression of a newly identified d-amino acid transaminase in Mycobacterium smegmatis complements glutamate racemase deletion. Molecular Microbiology, 107(2), 198-213. doi: 10.1111/mmi.13877
Lim, C. S., Wardell, S. J. T., Kleffmann, T., & Brown, C. M. (2018). The exon-intron gene structure upstream of the initiation codon predicts translation efficiency. Nucleic Acids Research, 46(9), 4575-4591. doi: 10.1093/nar/gky282
González-Miró, M., Rodríguez-Noda, L. M., Fariñas-Medina, M., Cedré-Marrero, B., Madariaga-Zarza, S., Zayas-Vignier, C., … Kleffmann, T., … Rehm, B. H. A. (2018). Bioengineered polyester beads co-displaying protein and carbohydrate-based antigens induce protective immunity against bacterial infection. Scientific Reports, 8, 1888. doi: 10.1038/s41598-018-20205-7
Rodger, E. J., Porteous, C. M., Jones, G. T., Legge, M., Kleffmann, T., & McCormick, S. P. A. (2018). Proteomic analysis of liver from lipoprotein(a) transgenic mice shows an oxidative stress and lipid export response. BioMed Research International, 2018, 4963942. doi: 10.1155/2018/4963942
Parakra, R. D., Kleffmann, T., Jameson, G. N. L., & Ledgerwood, E. C. (2018). The proportion of Met80-sulfoxide dictates peroxidase activity of human cytochrome c. Dalton Transactions, 47, 9128. doi: 10.1039/c8dt02185f
Tong, M., Kleffmann, T., Pradhan, S., Johansson, C. L., DeSousa, J., Stone, P. R., … Chamley, L. W. (2016). Proteomic characterization of macro-, micro- and nano-extracellular vesicles derived from the same first trimester placenta: Relevance for feto-maternal communication. Human Reproduction, 31(4), 687-699. doi: 10.1093/humrep/dew004
Muhsin-Sharafaldine, M.-R., Saunderson, S. C., Dunn, A. C., Faed, J. M., Kleffmann, T., & McLellan, A. D. (2016). Procoagulant and immunogenic properties of melanoma exosomes, microvesicles and apoptotic vesicles. Oncotarget, 7(35), 56279-56294. doi: 10.18632/oncotarget.10783
Wei, J., Lau, S. Y., Blenkiron, C., Chen, Q., James, J. L., Kleffmann, T., … Chamley, L. W. (2016). Trophoblastic debris modifies endothelial cell transcriptome in vitro: A mechanism by which fetal cells might control maternal responses to pregnancy. Scientific Reports, 6, 30632. doi: 10.1038/srep30632
Kohn, Y. Y., Symonds, J. E., Kleffmann, T., Nakagawa, S., Lagisz, M., & Lokman, P. M. (2015). Proteomic analysis of early-stage embryos: Implications for egg quality in hapuku (Polyprion oxygeneios). Fish Physiology & Biochemistry, 41(6), 1403-1417. doi: 10.1007/s10695-015-0095-0
Cardno, T. S., Shimaki, Y., Sleebs, B. E., Lackovic, K., Parisot, J. P., Moss, R. M., Crowe-McAuliffe, C., Mathew, S. F., Edgar, C. D., Kleffmann, T., & Tate, W. P. (2015). HIV-1 and human PEG10 frameshift elements are functionally distinct and distinguished by novel small molecule modulators. PLoS ONE, 10(10), e0139036. doi: 10.1371/journal.pone.0139036
Frampton, R. A., Lopez Acedo, E., Young, V. L., Chen, D., Tong, B., Taylor, C., Easingwood, R. A., … Kleffmann, T., Bostina, M., & Fineran, P. C. (2015). Genome, proteome and structure of a T7-like bacteriophage of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae. Viruses, 7(7), 3361-3379. doi: 10.3390/v7072776
Guy, P. L., Easingwood, R., Carne, D. L., & Kleffmann, T. (2015). Identification of two plant viruses using partial purification and mass spectrometry. Australasian Plant Disease Notes, 10, 11. doi: 10.1007/s13314-015-0162-5
von Zychlinski, A., & Kleffmann, T. (2015). Dissecting the proteome of lipoproteins: New biomarkers for cardiovascular diseases? Translational Proteomics, 7, 30-39. doi: 10.1016/j.trprot.2014.12.001
Pantham, P., Viall, C. A., Chen, Q., Kleffmann, T., Print, C. G., & Chamley, L. W. (2015). Antiphospholipid antibodies bind syncytiotrophoblast mitochondria and alter the proteome of extruded syncytial nuclear aggregates. Placenta, 36, 1463-1473. doi: 10.1016/j.placenta.2015.10.006
Tchesnokov, E. P., Fellner, M., Siakkou, E., Kleffmann, T., Martin, L. W., Aloi, S., Lamont, I. L., Wilbanks, S. M., & Jameson, G. N. L. (2015). The cysteine dioxygenase homologue from Pseudomonas aeruginosa is a 3-mercaptopropionate dioxygenase. Journal of Biological Chemistry, 290(40), 24424-24437. doi: 10.1074/jbc.M114.635672
Ramsay, J. P., Tester, L. G. L., Major, A. S., Sullivan, J. T., Edgar, C. D., Kleffmann, T., … Tate, W. P., … Ronson, C. W. (2015). Ribosomal frameshifting and dual-target antiactivation restrict quorum-sensing–activated transfer of a mobile genetic element. PNAS, 112(3), 4104-4109. doi: 10.1073/pnas.1501574112
Marchalant, Y., Brownjohn, P. W., Bonnet, A., Kleffmann, T., & Ashton, J. C. (2014). Validating antibodies to the cannabinoid CB2 receptor: Antibody sensitivity is not evidence of antibody specificity. Journal of Histochemistry & Cytochemistry, 62(6), 395-404. doi: 10.1369/0022155414530995
Pemberton, C. J., Siriwardena, M., Kleffmann, T., & Richards, A. M. (2014). C-type natriuretic peptide (CNP) signal peptide fragments are present in the human circulation. Biochemical & Biophysical Research Communications, 449, 301-306. doi: 10.1016/j.bbrc.2014.05.020
Johnson, S. L., Villarroel, M., Rosengrave, P., Carne, A., Kleffmann, T., Lokman, P. M., & Gemmell, N. J. (2014). Proteomic analysis of Chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. PLoS ONE, 9(8), e104155. doi: 10.1371/journal.pone.0104155
von Zychilinski, A., Williams, M., McCormick, S., & Kleffmann, T. (2014). Absolute quantification of apolipoproteins and associated proteins on human plasma lipoproteins. Journal of Proteomics, 106, 181-190. doi: 10.1016/j.jprot.2014.04.030
Kumar, V., Kleffmann, T., Hampton, M. B., Cannell, M. B., & Winterbourn, C. C. (2013). Redox proteomics of thiol proteins in mouse heart during ischemia/reperfusion using ICAT reagents and mass spectrometry. Free Radical Biology & Medicine, 58, 109-117. doi: 10.1016/j.freeradbiomed.2013.01.021
Souness, R. J., Kleffmann, T., Tchesnokov, E. P., Wilbanks, S. M., Jameson, G. B., & Jameson, G. N. L. (2013). Mechanistic implications of persulfenate and persulfide binding in the active site of cysteine dioxygenase. Biochemistry, 52(43), 7606-7617. doi: 10.1021/bi400661a
Nakatani, Y., Kleffmann, T., Linke, K., Condon, S. M., Hinds, M. G., & Day, C. L. (2013). Regulation of ubiquitin transfer by XIAP, a dimeric RING E3 ligase. Biochemical Journal, 450(3), 629-638. doi: 10.1042/BJ20121702
Pemberton, C. J., & Kleffmann, T. (2013). Circulating signal peptides: More than just a bus ticket? Cardiovascular Endocrinology, 2(3), 41-45. doi: 10.1097/XCE.0b013e328362e44e
Rodger, E. J., Suetani, R. J., Jones, G. T., Kleffmann, T., Carne, A., Legge, M., & McCormick, S. P. A. (2012). Proteomic analysis of aortae from human lipoprotein(a) transgenic mice shows an early metabolic response independent of atherosclerosis. PLoS ONE, 7(1), e30383. doi: 10.1371/journal.pone.0030383
Pemberton, C. J., Siriwardena, M., Kleffmann, T., Ruygrok, P., Palmer, S. C., Yandle, T. G., & Richards, A. M. (2012). First identification of circulating Prepro-A-Type natriuretic peptide (PreproANP) signal peptide fragments in humans: Initial assessment as cardiovascular biomarkers. Clinical Chemistry, 58(4), 757-767. doi: 10.1373/clinchem.2011.176990
von Zychlinski, A., Kleffmann, T., Williams, M. J. A., & McCormick, S. P. (2011). Proteomics of Lipoprotein(a) identifies a protein complement associated with response to wounding. Journal of Proteomics, 74, 2881-2891. doi: 10.1016/j.jprot.2011.07.008
Park, S. J., Kleffmann, T., & Hessian, P. A. (2011). The G82S polymorphism promotes glycosylation of the receptor for advanced glycation end products (RAGE) at Asn81: A comparison of wild-type RAGE with the G82S polymorphic variant. Journal of Biological Chemistry, 286(24), 21384-21392. doi: 10.1074/jbc.M111.241281
Wong, C. K., Young, V. L., Kleffmann, T., & Ward, V. K. (2011). Genomic and proteomic analysis of invertebrate iridovirus type 9. Journal of Virology, 85(15), 7900-7911. doi: 10.1128/jvi.00645-11
Kafka, A. P., Kleffmann, T., Rades, T., & McDowell, A. (2011). The application of MALDI TOF MS in biopharmaceutical research. International Journal of Pharmaceutics, 417(1-2), 70-82. doi: 10.1016/j.ijpharm.2010.12.010
Dickerhof, N., Kleffmann, T., Jack, R., & McCormick, S. (2011). Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain. FEBS Journal, 278(12), 2034-2043. doi: 10.1111/j.1742-4658.2011.08119.x
Kafka, A. P., Kleffmann, T., Rades, T., & McDowell, A. (2010). Characterization of peptide polymer interactions in poly(alkylcyanoacrylate) nanoparticles: A mass spectrometric approach. Current Drug Delivery, 7(3), 208-215.
Siriwardena, M., Kleffmann, T., Ruygrok, P., Cameron, V. A., Yandle, T. G., Nicholls, M. G., Richards, A. M., & Pemberton, C. J. (2010). B-type natriuretic peptide signal peptide circulates in human blood. Evaluation as a potential biomarker of cardiac ischemia. Circulation, 122, 255-264. doi: 10.1161/CIRCULATIONAHA.109.909937
Kleffmann, T., Jongkees, S. A. K., Fairweather, G., Wilbanks, S. M., & Jameson, G. N. L. (2009). Mass-spectrometric characterization of two posttranslational modifications of cysteine dioxygenase. Journal of Biological Inorganic Chemistry, 14, 913-921. doi: 10.1007/s00775-009-0504-x
Kafka, A. P., Kleffmann, T., Rades, T., & McDowell, A. (2009). Histidine residues in the peptide D-Lys6-GnRH: Potential for copolymerization in polymeric nanoparticles. Molecular Pharmaceutics, 6(5), 1483-1491. doi: 10.1021/mp900043e
Wirawan, R. E., Swanson, K. M., Kleffmann, T., Jack, R. W., & Tagg, J. R. (2007). Uberolysin: A novel cyclic bacteriocin produced by Streptococcus uberis. Microbiology, 153, 1619-1630.
Kleffmann, T., von Zychlinski, A., Russenberger, D., Hirsch-Hoffmann, M., Gehrig, P., Gruissem, W., & Baginsky, S. (2007). Proteome dynamics during plastid differentiation in rice. Plant Physiology, 143, 912-923. doi: 10.1104/pp.106.090738
Clark, M. B., Jänicke, M., Gottesbühren, U., Kleffmann, T., Legge, M., Poole, E. S., & Tate, W. P. (2007). A mammalian gene PEG10 expresses two reading frames by high efficiency -1 frameshifting in embryonic-associated tissues. Journal of Biological Chemistry, 282(52), 37359-37369.
Kleffmann, T., Hirsch-Hoffmann, M., Gruissem, W., & Baginsky, S. (2006). plprot: A comprehensive proteome database for different plastid types. Plant & Cell Physiology, 47(3), 432-436.
von Zychilinski, A., Kleffmann, T., Krishnamurthy, N., Sjolander, K., Baginsky, S., & Gruissem, W. (2005). Proteome analysis of the rice etioplast: Metabolic and regulatory networks and novel protein functions. Molecular & Cellular Proteomics, 4(8), 1072-1084.
Baginsky, S., Kleffmann, T., von Zychlinski, A., & Gruissem, W. (2005). Analysis of shotgun proteomics and RNA profiling data from Arabidopsis thaliana chloroplasts. Journal of Proteome Research, 4(2), 637-640.
Kleffmann, T., Russenberger, D., von Zychilinski, A., Sjolander, K., Gruissem, W., & Baginsky, S. (2004). The arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions. Current Biology, 14, 354-362.
Kollien, A. H., Grospietsch, T., Kleffmann, T., Zerbst-Boroffka, I., & Schaub, G. A. (2001). Ionic composition of the rectal contents and excreta of the reduviid bug Triatoma infestans. Journal of Insect Physiology, 47, 739-747.
Cabral, M. M. O., Azambuja, P., Gottlieb, O. R., Kleffmann, T., Garcia, E. S., & Schaub, G. A. (2001). Burchellin: Effects on Triatoma infestans and on Trypanosoma cruzi within this vector. Parasitology Research, 87, 730-735.
Cabral, M. M., Kollien, A. H., Kleffmann, T., Azambuja, P., Gottlieb, O. R., Garcia, E. S., & Schaub, G. A. (2000). Rhodnius prolixus: Effects of the neolignan burchellin on in vivo and in vitro diuresis. Parasitology Research, 86(9), 710-716.
Journal - Research Other
Pemberton, C. J., Richards, A. M., Siriwardena, M., Cameron, V. A., Yandle, T. G., Nicholls, M. G., Kleffmann, T., & Ruygrok, P. (2011). [Response to letter regarding article, B-Type natriuretic peptide signal peptide circulates in human blood: Evaluation as a potential biomarker of cardiac ischemia]. Circulation, 123(6), e234. doi: 10.1161/CIRCULATIONAHA.110.003574