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Department of Biochemistry profile

Professor Iain Lamont

PositionProfessor
DepartmentDepartment of Biochemistry
QualificationsBSc (Edin) DPhil (Oxon)
Research summaryMolecular basis of infectious disease
TeachingBiochemistry; genetics; molecular biology
Memberships
  • Institutional Biological Safety Committee
  • Genetics Coordinating Group
ClinicalInterests: cystic fibrosis; bronchiectasis

Research

Iain has a long-standing interest in the molecular genetics of bacteria that cause infectious disease.

He and his research team have a particular focus on the pathogenic bacterium Pseudomonas aeruginosa that causes a wide range of infections such as chronic infections in patients in cystic fibrosis.

During infection the bacteria secrete a number of agents that contribute to the pathogenic process. These include numerous proteins and an iron-scavenging compound called pyoverdine. Iain and his team are also using a wide range of biochemical, molecular biological, and genetic approaches to study the way in which pyoverdine contributes to infection and how it is made.

P. aeruginosa become very resistant to antibiotics during infection and highly resistant strains ("superbugs") are some of the most problematic infectious bacteria on the planet. Iain and his research team are using genetic, bioinformatic and molecular approaches to understand how P. aeruginosa becomes resistant to antibiotics with the long term goal of better management of infections.

Current research areas include:

  • Understanding antibiotic resistance
  • How does P. aeruginosa exist in cystic fibrosis?
  • Genomics and comparative genomics of P. aeruginosa
  • Regulation and enzymology of virulence factor production

Publications

Mastropasqua, M. C., Lamont, I., Martin, L. W., Reid, D. W., D'Orazio, M., & Battistoni, A. (2018). Efficient zinc uptake is critical for the ability of Pseudomonas aeruginosa to express virulence traits and colonize the human lung. Journal of Trace Elements in Medicine & Biology, 48, 74-80. doi: 10.1016/j.jtemb.2018.03.009

Edgar, R. J., Hampton, G. E., Casas Garcia, G. P., Maher, M. J., Perugini, M. A., Ackerley, D. F., & Lamont, I. L. (2017). Integrated activities of two alternative sigma factors coordinate iron acquisition and uptake by Pseudomonas aeruginosa. Molecular Microbiology, 106(6), 891-904. doi: 10.1111/mmi.13855

Bishop, T. F., Martin, L. W., & Lamont, I. L. (2017). Activation of a cell surface signaling pathway in Pseudomonas aeruginosa requires ClpP protease and new sigma factor synthesis. Frontiers in Microbiology, 8, 2442. doi: 10.3389/fmicb.2017.02442

Forester, N. T., Lane, G. A., Steringa, M., Lamont, I. L., & Johnson, L. J. (2017). Contrasting roles of fungal siderophores in maintaining iron homeostasis in Epichloë festucae. Fungal Genetics & Biology. Advance online publication. doi: 10.1016/j.fgb.2017.11.003

Hilliam, Y., Moore, M. P., Lamont, I. L., Bilton, D., Haworth, C. S., Foweraker, J., … Winstanley, C. (2017). Pseudomonas aeruginosa adaptation and diversification in the non-cystic fibrosis bronchiectasis lung. European Respiratory Journal, 49(4), 1602108. doi: 10.1183/13993003.02108-2016

Authored Book - Research

Brumby, A. M., Lamont, I. L., Dodd, I. B., Egan, J. B., Walling, G. A., McEwan, J. C., … Montgomery, G. (1996). Defining the SOS operon of coliphage 186. N/A, 114p.

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Journal - Research Article

Mastropasqua, M. C., Lamont, I., Martin, L. W., Reid, D. W., D'Orazio, M., & Battistoni, A. (2018). Efficient zinc uptake is critical for the ability of Pseudomonas aeruginosa to express virulence traits and colonize the human lung. Journal of Trace Elements in Medicine & Biology, 48, 74-80. doi: 10.1016/j.jtemb.2018.03.009

Edgar, R. J., Hampton, G. E., Casas Garcia, G. P., Maher, M. J., Perugini, M. A., Ackerley, D. F., & Lamont, I. L. (2017). Integrated activities of two alternative sigma factors coordinate iron acquisition and uptake by Pseudomonas aeruginosa. Molecular Microbiology, 106(6), 891-904. doi: 10.1111/mmi.13855

Hilliam, Y., Moore, M. P., Lamont, I. L., Bilton, D., Haworth, C. S., Foweraker, J., … Winstanley, C. (2017). Pseudomonas aeruginosa adaptation and diversification in the non-cystic fibrosis bronchiectasis lung. European Respiratory Journal, 49(4), 1602108. doi: 10.1183/13993003.02108-2016

Forester, N. T., Lane, G. A., Steringa, M., Lamont, I. L., & Johnson, L. J. (2017). Contrasting roles of fungal siderophores in maintaining iron homeostasis in Epichloë festucae. Fungal Genetics & Biology. Advance online publication. doi: 10.1016/j.fgb.2017.11.003

Bishop, T. F., Martin, L. W., & Lamont, I. L. (2017). Activation of a cell surface signaling pathway in Pseudomonas aeruginosa requires ClpP protease and new sigma factor synthesis. Frontiers in Microbiology, 8, 2442. doi: 10.3389/fmicb.2017.02442

Nunn, S., & Lamont, I. (2017). Beauty is the beast. Junctures, 2017(18), 100-105.

Germoni, L. A. P., Bremer, P. J., & Lamont, I. L. (2016). The effect of alginate lyase on the gentamicin resistance of Pseudomonas aeruginosa in mucoid biofilms. Journal of Applied Microbiology, 121(1), 126-135. doi: 10.1111/jam.13153

Freschi, L., Jeukens, J., Kukavica-Ibrulj, I., Boyle, B., Dupont, M.-J., LaRoche, J., … Lamont, I. L., … Levesque, R. C. (2015). Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium. Frontiers in Microbiology, 6, 1036. doi: 10.3389/fmicb.2015.01036

Donaldson, A. E., & Lamont, I. L. (2015). Metabolomics of post-mortem blood: Identifying potential markers of post-mortem interval. Metabolomics, 11(1), 237-245. doi: 10.1007/s11306-014-0691-5

Lopez-Medina, E., Fan, D., Coughlin, L. A., Ho, E. X., Lamont, I. L., Reimmann, C., … Koh, A. Y. (2015). Candida albicans inhibits Pseudomonas aeruginosa virulence through suppression of pyochelin and pyoverdine biosynthesis. PLoS Pathogens, 11(8), e1005129. doi: 10.1371/journal.ppat.1005129

Tchesnokov, E. P., Fellner, M., Siakkou, E., Kleffmann, T., Martin, L. W., Aloi, S., Lamont, I. L., Wilbanks, S. M., & Jameson, G. N. L. (2015). The cysteine dioxygenase homologue from Pseudomonas aeruginosa is a 3-mercaptopropionate dioxygenase. Journal of Biological Chemistry, 290(40), 24424-24437. doi: 10.1074/jbc.M114.635672

Edgar, R. J., Xu, X., Shirley, M., Konings, A. F., Martin, L. W., Ackerley, D. F., & Lamont, I. L. (2014). Interactions between an anti-sigma protein and two sigma factors that regulate the pyoverdine signaling pathway in Pseudomonas aeruginosa. BMC Microbiology, 14, 287. doi: 10.1186/s12866-014-0287-2

Donaldson, A. E., & Lamont, I. L. (2014). Estimation of post-mortem interval using biochemical markers. Australian Journal of Forensic Sciences, 46(1), 8-26. doi: 10.1080/00450618.2013.784356

Calcott, M. J., Owen, J. G., Lamont, I. L., & Ackerley, D. F. (2014). Biosynthesis of novel pyoverdines by domain substitution in a non-ribosomal peptide synthetase of Pseudomonas aeruginosa. Applied & Environmental Microbiology, 80(18), 5723-5731. doi: 10.1128/aem.01453-14

Llamas, M. A., Imperi, F., Visca, P., & Lamont, I. L. (2014). Cell-surface signaling in Pseudomonas: Stress responses, iron transport and pathogenicity. FEMS Microbiology Reviews, 38, 569-597. doi: 10.1111/1574-6976.12078

Nguyen, A. T., O'Neill, M. J., Watts, A. M., Robson, C. L., Lamont, I. L., Wilks, A., & Oglesby-Sherrouse, A. G. (2014). Adaptation of iron homeostasis pathways by a Pseudomonas aeruginosa pyoverdine mutant in the cystic fibrosis lung. Journal of Bacteriology, 196(12), 2265-2276. doi: 10.1128/JB.01491-14

Smith, D. J., Lamont, I. L., Anderson, G. J., & Reid, D. W. (2013). Targeting iron uptake to control Pseudomonas aeruginosa infections in cystic fibrosis. European Respiratory Journal, 42(6), 1723-1736. doi: 10.1183/09031936.00124012

Donaldson, A. E., & Lamont, I. L. (2013). Biochemistry changes that occur after death: Potential markers for determining post-mortem interval. PLoS ONE, 8(11), e82011. doi: 10.1371/journal.pone.0082011

Butler, M. I., Stockwell, P. A., Black, M. A., Day, R. C., Lamont, I. L., & Poulter, R. T. M. (2013). Pseudomonas syringae pv. actinidiae from recent outbreaks of kiwifruit bacterial canker belong to different clones that originated in China. PLoS ONE, 8(2), e57464. doi: 10.1371/journal.pone.0057464

Konings, A. F., Martin, L. W., Sharples, K. J., Roddam, L. F., Latham, R., Reid, D. W., & Lamont, I. L. (2013). Pseudomonas aeruginosa uses multiple pathways to acquire iron during chronic infection in cystic fibrosis lungs. Infection & Immunity, 81(8), 2697-2704. doi: 10.1128/IAI.00418-13

Reid, D. W., Latham, R., Lamont, I. L., Camara, M., & Roddam, L. F. (2013). Molecular analysis of changes in Pseudomonas aeruginosa load during treatment of a pulmonary exacerbation in cystic fibrosis. Journal of Cystic Fibrosis, 12, 688-699. doi: 10.1016/j.jcf.2013.03.008

Draper, R. C., Martin, L. W., Beare, P. A., & Lamont, I. L. (2011). Differential proteolysis of sigma regulators controls cell-surface signaling in Pseudomonas aeruginosa. Molecular Microbiology, 82(6), 1444-1453. doi: 10.1111/j.1365-2958.2011.07901.x

Donaldson, A. E., Walker, N. K., Lamont, I. L., Cordiner, S. J., & Taylor, M. C. (2011). Characterising the dynamics of expirated bloodstain pattern formation using high-speed digital video imaging. International Journal of Legal Medicine, 125, 757-762. doi: 10.1007/s00414-010-0498-5

Martin, L. W., Reid, D. W., Sharples, K. J., & Lamont, I. L. (2011). Pseudomonas siderophores in the sputum of patients with cystic fibrosis. Biometals, 24(6), 1059-1067. doi: 10.1007/s10534-011-9464-z

Upritchard, H. G., Yang, J., Bremer, P. J., Lamont, I. L., & McQuillan, A. J. (2011). Adsorption of enterobactin to metal oxides and the role of siderophores in bacterial adhesion to metals. Langmuir, 27(17), 10587-10596. doi: 10.1021/la202352j

Nakatani, Y., Lamont, I. L., & Cutfield, J. F. (2010). Discovery and characterization of a distinctive exo-1,3/1,4-β-glucanase from the marine bacterium Pseudoalteromonas sp. strain BB1. Applied & Environmental Microbiology, 76(20), 6760-6768. doi: 10.1128/AEM.00758-10

McMillan, D. G. G., Velasquez, I., Nunn, B. L., Goodlett, D. R., Hunter, K. A., Lamont, I., Sander, S. G., & Cook, G. M. (2010). Acquisition of iron by alkaliphilic Bacillus species. Applied & Environmental Microbiology, 76(20), 6955-6961. doi: 10.1128/AEM.01393-10

Donaldson, A., Taylor, M. C., Cordiner, S. J., & Lamont, I. L. (2010). Using oral microbial DNA analysis to identify expirated bloodspatter. International Journal of Legal Medicine, 124(6), 569-576. doi: 10.1007/s00414-010-0426-8

Yeterian, E., Martin, L. W., Lamont, I. L., & Schalk, I. J. (2010). An efflux pump is required for siderophore recycling by Pseudomonas aeruginosa. Environmental Microbiology Reports, 2(3), 412-418. doi: 10.1111/j.1758-2229.2009.00115.x

Yeterian, E., Martin, L. W., Guillon, L., Journet, L., Lamont, I. L., & Schalk, I. J. (2010). Synthesis of the siderophore pyoverdine in Pseudomonas aeruginosa involves a periplasmic maturation. Amino Acids, 38(5), 1447-1459. doi: 10.1007/s00726-009-0358-0

Shirley, M., & Lamont, I. L. (2009). Role of TonB1 in pyoverdine-mediated signaling in Pseudomonas aeruginosa. Journal of Bacteriology, 191(18), 5634-5640. doi: 10.1128/JB.00742-09

Lamont, I. L., Konings, A. F., & Reid, D. W. (2009). Iron acquisition by Pseudomonas aeruginosa in the lungs of patients with cystic fibrosis. Biometals, 22(1), 53-60. doi: 10.1007/s10534-008-9197-9

Reid, D. W., Anderson, G. J., & Lamont, I. L. (2009). Role of lung iron in determining the bacterial and host struggle in cystic fibrosis. American Journal of Physiology: Lung Cellular & Molecular Physiology, 297(5), L795-L802. doi: 10.1152/ajplung.00132.2009

Schalk, I. J., Lamont, I. L., & Cobessi, D. (2009). Structure-function relationships in the bifunctional ferrisiderophore FpvA receptor from Pseudomonas aeruginosa. Biometals, 22(4), 671-678. doi: 10.1007/s10534-008-9203-2

Mettrick, K. A., & Lamont, I. L. (2009). Different roles for anti-sigma factors in siderophore signalling pathways of Pseudomonas aeruginosa. Molecular Microbiology, 74(5), 1257-1271. doi: 10.1111/j.1365-2958.2009.06932.x

Ramsay, J. P., Sullivan, J. T., Jambari, N., Ortori, C. A., Heeb, S., Williams, P., … Lamont, I. L., & Ronson, C. W. (2009). A LuxRI-family regulatory system controls excision and transfer of the Mesorhizobium loti strain R7A symbiosis island by activating expression of two conserved hypothetical genes. Molecular Microbiology, 73(6), 1141-1155. doi: 10.1111/j.1365-2958.2009.06843.x

Spencer, M. R., Beare, P. A., & Lamont, I. L. (2008). Role of cell surface signaling in proteolysis of an alternative sigma factor in Pseudomonas aeruginosa. Journal of Bacteriology, 190(14), 4865-4869.

Upritchard, H. G., Cordwell, S. J., & Lamont, I. L. (2008). Immunoproteomics to examine cystic fibrosis host interactions with extracellular Pseudomonas aeruginosa proteins. Infection & Immunity, 76(10), 4624-4632. doi: 10.1128/IAI.01707-07

Upritchard, H. G., Yang, J., Bremer, P. J., Lamont, I. L., & McQuillan, A. J. (2007). Adsorption to metal oxides of the Pseudomonas aeruginosa siderophore pyoverdine and implications for bacterial biofilm formation on metals. Langmuir, 23(13), 7189-7195.

Visca, P., Imperi, F., & Lamont, I. L. (2007). Pyoverdine siderophores: From biogenesis to biosignificance. Trends in Microbiology, 15(1), 22-30.

Carlton, T. M., Sullivan, J. T., Stuart, G. S., Hutt, K., Lamont, I. L., & Ronson, C. W. (2007). Ferrichrome utilization in a mesorhizobial population: Microevolution of a three-locus system. Environmental Microbiology, 9(12), 2923-2932.

Diggle, S. P., Matthijs, S., Wright, V. J., Fletcher, M. P., Chhabra, S. R., Lamont, I. L., … Williams, P. (2007). The Pseudomonas aeruginosa 4-quinolone signal molecules HHQ and PQS play multifunctional roles in quorum sensing and iron entrapment. Chemistry & Biology, 14, 87-96.

Yang, J., Bremer, P. J., Lamont, I. L., & McQuillan, A. J. (2006). Infrared spectroscopic studies of siderophore-related hydroxamic acid ligands adsorbed on titanium dioxide. Langmuir, 22(24), 10109-10117.

Lamont, I. L., Martin, L. W., Sims, T., Scott, A., & Wallace, M. (2006). Characterization of a gene encoding an acetylase required for pyoverdine synthesis in Pseudomonas aeruginosa. Journal of Bacteriology, 188(8), 3149-3152. doi: 10.1128/JB.188.8.3149-3152.2006

Ramsay, J. P., Sullivan, J. T., Stuart, G. S., Lamont, I. L., & Ronson, C. W. (2006). Excision and transfer of the Mesorhizobium loti R7A symbiosis island requires an integrase IntS, a novel recombination directionality factor RdfS, and a putative relaxase RlxS. Molecular Microbiology, 62(3), 723-734. doi: 10.1111/j.1365-2958.2006.05396.x

Wilson, M. J., & Lamont, I. L. (2006). Mutational analysis of an extracytoplasmic-function sigma factor to investigate its interactions with RNA polymerase and DNA. Journal of Bacteriology, 188(5), 1935-1942.

James, H. E., Beare, P. A., Martin, L. W., & Lamont, I. L. (2005). Mutational analysis of a bifunctional ferrisiderophore receptor and signal-transducing protein from Pseudomonas aeruginosa. Journal of Bacteriology, 187(13), 4514-4520.

Ackerley, D. F., & Lamont, I. L. (2004). Characterization and genetic manipulation of peptide synthetases in Pseudomonas aeruginosa PAO1 in order to generate novel pyoverdines. Chemistry & Biology, 11, 971-980.

Caradoc-Davies, T. T., Cutfield, S. M., Lamont, I. L., & Cutfield, J. F. (2004). Crystal structures of Escherichia coli uridine phosphorylase in two native and three complexed forms reveal basis of substrate specificity, induced conformational changes and influence of potassium. Journal of Molecular Biology, 337, 337-354.

McWhirter, M. J., Bremer, P. J., Lamont, I. L., & McQuillan, A. J. (2003). Siderophore-mediated covalent bonding to metal (oxide) surfaces during biofilm initiation by Pseudomonas aeruginosa bacteria. Langmuir, 19, 3575-3577.

Ackerley, D. F., Caradoc-Davies, T. T., & Lamont, I. L. (2003). Substrate specificity of the nonribosomal peptide synthetase PvdD from Pseudomonas aeruginosa. Journal of Bacteriology, 185(9), 2848-2855.

Lamont, I. L., & Martin, L. (2003). Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa. Microbiology, 149, 833-842.

Beare, P., For, R. J., Martin, L., & Lamont, I. L. (2003). Siderophore-mediated cell signalling in Pseudomonas aeruginosa: Divergent pathways regulate virulence factor production and siderophore receptor synthesis. Molecular Microbiology, 47(1), 195-207.

Visca, P., Leoni, L., Wilson, M. J., & Lamont, I. L. (2002). Iron transport and regulation, cell signalling and genomics: Lessons from Escherichia coli and Pseudomonas. Molecular Microbiology, 45(5), 1177-1190.

Adhikari, R. P., Cook, G. M., Lamont, I., Lang, S., Heffernan, H., & Smith, J. M. B. (2002). Phenotypic and molecular characterization of community occurring, Western Samoan phage pattern methicillin-resistant Staphyococcus aureus. Journal of Antimicrobial Chemotherapy, 50, 825-831.

Lamont, I. L., Beare, P. A., Ochsner, U., Vasil, A., & Vasil, M. L. (2002). Siderophore-mediated signaling regulates virulence factor production in Pseudomonas aeruginosa. PNAS, 99(10), 7072-7077.

Wilderman, P. J., Vasil, A. I., Johnson, Z., Wilson, M. J., Cunliffe, H. E., Lamont, I. L., & Vasil, M. L. (2001). Characterization of an endoprotease (PrpL) encoded by a PvdS-regulated gene in Pseudomonas aeruginosa. Infection & Immunity, 69(9), 5385-5394.

McMorran, B. J., Shantha Kumara, H. M., Sullivan, K., & Lamont, I. L. (2001). Involvement of a transformylase enzyme in siderophore synthesis in Pseudomonas aeruginosa. Microbiology, 147, 1-8.

Wilson, M. J., McMorran, B. J., & Lamont, I. L. (2001). Analysis of promoters recognized by PvdS, an extracytoplasmic function sigma factor protein from Pseudomonas aeruginosa. Journal of Bacteriology, 183(6), 2151-2155.

Wilson, M. J., & Lamont, I. L. (2000). Characterization of an ECF sigma factor protein from Pseudomonas aeruginosa. Biochemical & Biophysical Research Communications, 273(2), 578-583.

Al-Samarrai, T. H., Zhang, N. X., Lamont, I. L., Martin, L., Kolbe, J., Wilsher, M., … Schmid, J. (2000). Simple and inexpensive but highly discriminating method of computer-assisted DNA fingerprinting of Pseudomonas aeruginosa. Journal of Clinical Microbiology, 38(12), 4445-4452.

Rae, J. L., Cutfield, J. F., & Lamont, I. L. (1997). Sequences and expression of pyruvate dehydrogenase genes from Pseudomonas aeruginosa. Journal of Bacteriology, 179(11), 3561-3571.

Ochsner, O. A., Johnson, Z., Lamont, I. L., Cunliffe, H. E., & Vasil, M. L. (1996). Exotoxin A production in Pseudomonas aeruginosa requires the iron-regulated pvdS gene encoding an alternative sigma factor. Molecular Microbiology, 21(5), 1019-1028. doi: 10.1046/j.1365-2958.1996.481425.x

McMorran, B. J., Merriman, M. E., Rombel, I. T., & Lamont, I. L. (1996). Characterisation of the pvdE gene which is required for pyoverdine synthesis in Pseudomonas aeruginosa. Gene, 176(1/2), 55-59.

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Journal - Research Other

Smith, D. J., Anderson, G. J., Lamont, I. L., Masel, P., Campbell, S. C., & Reid, D. W. (2013). Accurate assessment of systemic iron status in cystic fibrosis will avoid the hazards of inappropriate iron supplementation. Journal of Cystic Fibrosis, 12(3), 303-304. doi: 10.1016/j.jcf.2012.10.001

Lamont, I. L., & Beare, P. (2003). Iron uptake, cell signalling and gene expression in Pseudomonas aeruginosa. New Zealand BioScience, 12(4), 15-16.

Lamont, I. L. (2003). Bacterial intercellular signalling and infectious disease. Australian Biochemist, 34(2), 4-6.

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