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Department of Biochemistry profile

Professor Kurt Krause

PositionProfessor
DepartmentDepartment of Biochemistry
QualificationsMA PhD MD
Research summaryStructural biology of infectious diseases

Research

Kurt Krause is a Professor of Biochemistry at the University of Otago.

He has a longstanding interest in structure and function in biology and his research areas include the structure and function of enzymes and proteins important in infectious diseases, such as bacterial pathogenesis factors, antibiotic targets, viral immunomodulatory proteins, and bioluminescence related proteins.

Publications

Campbell, A. C., Tanner, J. J., & Krause, K. L. (2021). Optimisation of neuraminidase expression for use in drug discovery by using HEK293-6E cells. Viruses, 13, 1893. doi: 10.3390/v13101893

Torbati, E., Krause, K. L., & Ussher, J. E. (2021). The immune response to SARS-CoV-2 and variants of concern. Viruses, 13, 1911. doi: 10.3390/v13101911

Badart, M. P., Barnes, E. M., Cording, A. P., Gilmer, S. C. L., Billinghurst, I. D., Edupuganti, V. V. S. R., … Bland, A. R., Bower, R. L., Rana, Z., Ferguson, S. A., Opel Reading, H. K., Cook, G. M., Rosengren, R. J., Krause, K. L., Gamble, A. B., Ashton, J. C., & Hawkins, B. C. (2021). Synthesis and biological evaluation of (-) and (+)-spiroleucettadine and analogues. ChemMedChem, 16, 1308-1315. doi: 10.1002/cmdc.202000954

Safarian, S., Opel-Reading, H. K., Wu, D., Mehdipour, A. R., Hards, K., Harold, L. K., … Stewart, I., … Cook, G. M., Krause, K. L., & Michel, H. (2021). The cryo-EM structure of the bd oxidase from M. tuberculosis reveals a unique structural framework and enables rational drug design to combat TB. Nature Communications, 12(1), 5236. doi: 10.1038/s41467-021-25537-z

McDonald, S. K., Matisoo-Smith, E. A., Buckley, H. R., Walter, R. K., Aung, H. L., Collins, C. J., Cook, G. M., Kardailsky, O., Krause, K., & Knapp, M. (2020). 'TB or not TB': The conundrum of pre-European contact tuberculosis in the Pacific. Philosophical Transactions of the Royal Society B, 375, 20190583. doi: 10.1098/rstb.2019.0583

Fagerlund, R. D., Ferguson, T. J., Maxwell, H. W. R., Opel-Reading, H. K., Krause, K. L., & Fineran, P. C. (2019). Reconstitution of CRISPR adaptation in vitro and its detection by PCR. In S. Bailey (Ed.), Methods in enzymology (Vol. 616: CRISPR-Cas enzymes). (pp. 411-433). Cambridge, MA: Academic Press. doi: 10.1016/bs.mie.2018.10.024

Chapter in Book - Research

Sharpe, M. L., Hastings, J. W., & Krause, K. L. (2014). Luciferases and light-emitting accessory proteins: Structural biology. In eLS. Chichester, UK: John Wiley & Sons. doi: 10.1002/9780470015902.a0003064.pub2

Chapter in Book - Research

Hastings, J. W., & Krause, K. L. (2006). Luciferases and light-emitting accessory proteins: Structural biology. In Encyclopedia of Life Sciences. New York: John Wiley & Sons. doi: 10.1038/npg.els.0003064

Chapter in Book - Research

Hastings, J. W., & Krause, K. L. (2005). Luciferase and light-emitting accessory proteins: Structural biology. In Encyclopedia of life sciences. John Wiley & Sons. doi: 10.1038/npg.els.0003064

Chapter in Book - Research

Krause, K. L., & Miller, M. D. (2002). A new engine for cleaving nucleic acid. In G. R. Eaton, D. C. Wiley & O. Jardetzky (Eds.), Structures and mechanisms: From ashes to enzymes. (pp. 270-293). Washington, DC, USA: American Chemical Society.

Chapter in Book - Research

Krause, K. L., & Miller, M. D. (2001). Using electrostatics to define the active site of Serratia endonuclease. In C. H. Schein (Ed.), Methods in molecular biology, Volume 160: Nuclease methods and protocols. (pp. 249-262). Totowa, NJ, USA: Humana Press.

Chapter in Book - Research

Hastings, J. W., & Krause, K. L. (2007). Luciferases and light-emitting accessory proteins: Structural biology. In Encyclopedia of Life Sciences. New York: John Wiley & Sons.

Chapter in Book - Other

Badart, M. P., Barnes, E. M., Cording, A. P., Gilmer, S. C. L., Billinghurst, I. D., Edupuganti, V. V. S. R., … Bland, A. R., Bower, R. L., Rana, Z., Ferguson, S. A., Opel Reading, H. K., Cook, G. M., Rosengren, R. J., Krause, K. L., Gamble, A. B., Ashton, J. C., & Hawkins, B. C. (2021). Synthesis and biological evaluation of (-) and (+)-spiroleucettadine and analogues. ChemMedChem, 16, 1308-1315. doi: 10.1002/cmdc.202000954

Journal - Research Article

Campbell, A. C., Tanner, J. J., & Krause, K. L. (2021). Optimisation of neuraminidase expression for use in drug discovery by using HEK293-6E cells. Viruses, 13, 1893. doi: 10.3390/v13101893

Journal - Research Article

Safarian, S., Opel-Reading, H. K., Wu, D., Mehdipour, A. R., Hards, K., Harold, L. K., … Stewart, I., … Cook, G. M., Krause, K. L., & Michel, H. (2021). The cryo-EM structure of the bd oxidase from M. tuberculosis reveals a unique structural framework and enables rational drug design to combat TB. Nature Communications, 12(1), 5236. doi: 10.1038/s41467-021-25537-z

Journal - Research Article

Kvach, M. V., Barzak, F. M., Harjes, S., Schares, H. A. M., Kurup, H. M., Jones, K. F., … Krause, K. L., … Filichev, V. (2020). Differential inhibition of APOBEC3 DNA-mutator isozymes by fluoro- versus non-fluoro-substituted 2'-deoxyzebularine embedded in single-stranded DNA. ChemBioChem, 21, 1028-1035. doi: 10.1002/cbic.201900505

Journal - Research Article

Murphy, E. L., Singh, K. V., Avila, B., Kleffmann, T., Gregory, S. T., Murray, B. E., Krause, K. L., … Jogl, G. (2020). Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis. Scientific Reports, 10, 16301. doi: 10.1038/s41598-020-73199-6

Journal - Research Article

Opel-Reading, H. K., Mortuza, R., & Krause, K. L. (2019). Detection of D-glutamate production from the dual function enzyme, 4-amino-4-deoxychorismate lyase/D-amino acid transaminase, in Mycobacterium smegmatis. Bio-protocol, 9(1), e3135. doi: 10.21769/BioProtoc.3135

Journal - Research Article

Mortuza, R., Aung, H. L., Taiaroa, G., Opel-Reading, H. K., Kleffmann, T., Cook, G. M., & Krause, K. L. (2018). Overexpression of a newly identified d-amino acid transaminase in Mycobacterium smegmatis complements glutamate racemase deletion. Molecular Microbiology, 107(2), 198-213. doi: 10.1111/mmi.13877

Journal - Research Article

Watkins, O. C., Sharpe, M. L., Perry, N. B., & Krause, K. L. (2018). New Zealand glowworm (Arachnocampa luminosa) bioluminescence is produced by a firefly-like luciferase but an entirely new luciferin. Scientific Reports, 8(3278). doi: 10.1038/s41598-018-21298-w

Journal - Research Article

Christensen, E. M., Patel, S. M., Korasick, D. A., Campbell, A. C., Krause, K. L., Becker, D. F., & Tanner, J. J. (2017). Resolving the cofactor-binding site in the proline biosynthetic enzyme human pyrroline-5-carboxylate reductase 1. Journal of Biological Chemistry, 292(17), 7233-7243. doi: 10.1074/jbc.M117.780288

Journal - Research Article

Fagerlund, R. D., Wilkinson, M. E., Klykov, O., Barendregt, A., Pearce, F. G., Kieper, S. N., Maxwell, H. W. R., … Krause, K. L., Bostina, M., … Staals, R. H. J., & Fineran, P. C. (2017). Spacer capture and integration by a type I-F Cas1–Cas2-3 CRISPR adaptation complex. PNAS, 114(26), E5122-E5128. doi: 10.1073/pnas.1618421114

Journal - Research Article

Nakatani, Y., Opel-Reading, H. K., Merker, M., Machado, D., Andres, S., Kumar, S. S., … Aung, H. L., Cook, G. M., … Krause, K., & Köser, C. U. (2017). Role of alanine racemase mutations in Mycobacterium tuberculosis D-cycloserine resistance. Antimicrobial Agents & Chemotherapy, 61(12), e01575-17. doi: 10.1128/aac.01575-17

Journal - Research Article

Poen, S., Nakatani, Y., Opel-Reading, H. K., Lassé, M., Dobson, R. C. J., & Krause, K. L. (2016). Exploring the structure of glutamate racemase from Mycobacterium tuberculosis as a template for anti-mycobacterial drug discovery. Biochemical Journal, 473, 1267-1280. doi: 10.1042/bcj20160186

Journal - Research Article

Sharif, S., Nakatani, Y., Wise, L., Corbett, M., Real, N. C., Stuart, G. S., Lateef, Z., Krause, K., Mercer, A. A., & Fleming, S. B. (2016). A broad-spectrum chemokine-binding protein of bovine papular stomatitis virus inhibits neutrophil and monocyte infiltration in inflammatory and wound models of mouse skin. PLoS ONE, 11(12), e0168007. doi: 10.1371/journal.pone.0168007

Journal - Research Article

Sowerby, S. J., Crump, J. A., Johnstone, M. C., Krause, K. L., & Hill, P. C. (2016). Smartphone microscopy of parasite eggs accumulated into a single field of view. American Journal of Tropical Medicine & Hygiene, 94(1), 227-230. doi: 10.4269/ajtmh.15-0427

Journal - Research Article

Wilkinson, M. E., Nakatani, Y., Staals, R. H. J., Kieper, S. N., Opel-Reading, H. K., McKenzie, R. E., Fineran, P. C., & Krause, K. L. (2016). Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system. Biochemical Journal, 473, 1063-1072. doi: 10.1042/bcj20160078

Journal - Research Article

Couñago, R. M., Knapp, K. M., Nakatani, Y., Fleming, S. B., Corbett, M., Wise, L. M., Mercer, A. A., & Krause, K. L. (2015). Structures of orf virus chemokine binding protein in complex with host chemokines reveal clues to broad binding specificity. Structure, 23(7), 1199-1213. doi: 10.1016/j.str.2015.04.023

Journal - Research Article

Sharpe, M. L., Dearden, P. K., Gimenez, G., & Krause, K. L. (2015). Comparative RNA seq analysis of the New Zealand glowworm Arachnocampa luminosa reveals bioluminescence-related genes. BMC Genomics, 16, 825. doi: 10.1186/s12864-015-2006-2

Journal - Research Article

Davis, E., Scaletti-Hutchinson, E., Opel-Reading, H., Nakatani, Y., & Krause, K. L. (2014). The structure of alanine racemase from Acinetobacter baumannii. Acta Crystallographica Section F, 70(9), 1199-1205.

Journal - Research Article

Li, Y., Mortuza, R., Milligan, D. L., Tran, S. L., Strych, U., Cook, G. M., & Krause, K. L. (2014). Investigation of the essentiality of glutamate racemase in Mycobacterium smegmatis. Journal of Bacteriology, 196(24), 4239-4244. doi: 10.1128/jb.02090-14

Journal - Research Article

Baker, E. S., Luckner, S. R., Krause, K. L., Lambden, P. R., Clarke, I. N., & Ward, V. K. (2012). Inherent structural disorder and dimerisation of murine norovirus NS1-2 protein. PLoS ONE, 7(2), e30534. doi: 10.1371/journal.pone.0030534

Journal - Research Article

Scaletti, E. R., Luckner, S. R., & Krause, K. L. (2012). Structural features and kinetic characterization of alanine racemase from Staphylococcus aureus (Mu50). Acta Crystallographica Section D, 68, 82-92. doi: 10.1107/S0907444911050682

Journal - Research Article

Anthony, K. G., Strych, U., Yeung, K. R., Shoen, C. S., Perez, O., Krause, K. L., … Koski, R. A. (2011). New classes of alanine racemase inhibitors identified by high-throughput screening show antimicrobial activity against Mycobacterium tuberculosis. PLoS ONE, 6(5), e20374. doi: 10.1371/journal.pone.0020374

Journal - Research Article

Im, H., Sharpe, M. L., Strych, U., Davlieva, M., & Krause, K. L. (2011). The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design. BMC Microbiology, 11, 116. doi: 10.1186/1471-2180-11-116

Journal - Research Article

Couñago, R. M., Fleming, S. B., Mercer, A. A., & Krause, K. L. (2010). Crystallization and preliminary X-ray analysis of the chemokine-binding protein from orf virus (Poxviridae). Acta Crystallographica Section F, F66, 819-823. doi: 10.1107/S1744309110018166

Journal - Research Article

Chen, C., Krause, K., & Pettitt, B. M. (2009). Advantage of being a dimer for Serratia marcescens endonuclease. Journal of Physical Chemistry B, 113(2), 511-521. doi: 10.1021/jp8057838

Journal - Research Article

Couñago, R. M., Davlieva, M., Strych, U., Hill, R. E., & Krause, K. L. (2009). Biochemical and structural characterization of alanine racemase from Bacillus anthracis (ames). BMC Structural Biology, 9, 53. doi: 10.1186/1472-6807-9-53

Journal - Research Article

Chen, C., Beck, B. W., Krause, K., Weksberg, T. E., & Pettitt, B. M. (2007). Effects of dimerization of Serratia marcescens endonuclease on water dynamics. Biopolymers, 85(3), 241-252.

Journal - Research Article

Milligan, D. L., Tran, S. L., Strych, U., Cook, G. M., & Krause, K. L. (2007). The alanine racemase of Mycobacterium smegmatis is essential for growth in the absence of D-alanine. Journal of Bacteriology, 189(22), 8381-8386.

Journal - Research Article

Strych, U., Davlieva, M., Longtin, J. P., Murphy, E. L., Im, H., Benedik, M. J., & Krause, K. L. (2007). Purification and preliminary crystallization of alanine racemase from Streptococcus pneumoniae. BMC Microbiology, 7, 40. doi: 10.1186/1471-2180-7-40

Journal - Research Article

Weksberg, T. E., Lynch, G. C., Krause, K. L., & Pettitt, B. M. (2007). Molecular dynamics simulations of Trichomonas vaginalis ferredoxin show a loop-cap transition. Biophysical Journal, 92(10), 3337-3345.

Journal - Research Article

LeMagueres, P., Im, H., Ebalunode, J., Strych, U., Benedik, M. J., Briggs, J. M., … Krause, K. L. (2005). The 1.9 Å crystal structure of alanine racemase from Mycobacterium tuberculosis contains a conserved entryway into the active site. Biochemistry, 44, 1471-1481.

Journal - Research Article

Shelburne, S. A., Visnegarwala, F., Adams, C., Krause, K. L., Hamill, R. J., & White, A. C. (2005). Unusual manifestations of disseminated Histoplasmosis in patients responding to antiretroviral therapy. American Journal of Medicine, 118, 1038-1041.

Journal - Research Article

Kim, M. G., Strych, U., Krause, K., Benedik, M., & Kohn, H. (2003). N(2)-substituted D,L-cycloserine derivatives: Synthesis and evaluation as alanine racemase inhibitors. Journal of Antibiotics, 56(2), 160-168.

Journal - Research Article

Kim, M. G., Strych, U., Krause, K., Benedik, M., & Kohn, H. (2003). Evaluation of amino-substituted heterocyclic derivatives as alanine racemase inhibitors. Medicinal Chemistry Research, 12(3), 130-138.

Journal - Research Article

LeMagueres, P., Im, H., Dvorak, A., Strych, U., Benedik, M., & Krause, K. L. (2003). Crystal structure at 1.45 Å resolution of alanine racemase from a pathogenic bacterium, Pseudomonas aeruginosa, contains both internal and external aldimine forms. Biochemistry, 42(50), 14752-14761.

Journal - Research Article

Liu, L., Im, H., Cegielski, M., LeMagueres, P., Schultz, L. W., Krause, K. L., & Hastings, J. W. (2003). Characterization and crystallization of active domains of a novel luciferase from a marine dinoflagellate. Acta Crystallographica Section D, D59, 761-764.

Journal - Research Article

Vidakovic, M., Crossnoe, C. R., Neidre, C., Kim, K., Krause, K. L., & Germanas, J. P. (2003). Reactivity of reduced [2Fe-2S] Ferredoxins parallels host susceptibility to nitroimidazoles. Antimicrobial Agents & Chemotherapy, 47(1), 302-308.

Journal - Research Article

Crossnoe, C. R., Germanas, J. P., LeMagueres, P., Mustata, G., & Krause, K. L. (2002). The crystal structure of Trichomonas vaginalis ferredoxin provides insight into metronidazole activation. Journal of Molecular Biology, 318(2), 503-518.

Journal - Research Article

Musher, D. M., Dowell, M. E., Shortridge, V. D., Flamm, R. K., Jorgensen, J. H., Le Magueres, P., & Krause, K. L. (2002). Emergence of macrolide resistance during treatment of pneumococcal pneumonia. New England Journal of Medicine, 346(8), 630-631.

Journal - Research Article

Strych, U., Penland, R. L., Jimenez, M., Krause, K. L., & Benedik, M. J. (2001). Characterization of the alanine racemases from two Mycobacteria. FEMS Microbiology Letters, 196, 93-98.

Journal - Research Article

Strych, U., Huang, H. C., Krause, K. L., & Benedik, M. J. (2000). Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1. Current Microbiology, 41, 290-294.

Journal - Research Article

Torbati, E., Krause, K. L., & Ussher, J. E. (2021). The immune response to SARS-CoV-2 and variants of concern. Viruses, 13, 1911. doi: 10.3390/v13101911

Journal - Research Other

Krause, K. L., Furneaux, R., Benjes, P., Brimble, M., Davidson, T., Denny, W., … Ussher, J. E., & Ward, V. (2020). The post-lockdown period should be used to acquire effective therapies for future resurgence in SARS-Cov-2 infections. New Zealand Medical Journal, 133(1513), 107-111. Retrieved from https://www.nzma.org.nz/journal

Journal - Research Other

McDonald, S. K., Matisoo-Smith, E. A., Buckley, H. R., Walter, R. K., Aung, H. L., Collins, C. J., Cook, G. M., Kardailsky, O., Krause, K., & Knapp, M. (2020). 'TB or not TB': The conundrum of pre-European contact tuberculosis in the Pacific. Philosophical Transactions of the Royal Society B, 375, 20190583. doi: 10.1098/rstb.2019.0583

Journal - Research Other

Chen, C., Beck, B. W., Krause, K., & Pettitt, B. M. (2006). Solvent participation in Serratia marcescens endonuclease complexes. Proteins, 62, 982-995.

Journal - Research Other

Krause, K. L. (2003). Review of the Nanotech 2003 - Nanotechnology Conference and Trade Show. IDrugs, 6(4), 309-311.

Journal - Research Other

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