Associate Professor Matloob Husain
| Position | Associate Professor |
|---|---|
| Department | Department of Microbiology and Immunology |
| Qualifications | MSc, PhD |
| Research summary | Molecular virology, viral pathogenesis and epidemiology |
Research
Matloob Husain is an Associate Professor in Virology.
Influenza virus remains one of the most circulated viruses and flu remains one of the most common infectious diseases in humans, globally. This burden of influenza virus on global public health may even get worse with the co-circulation and co-infection of pandemic SARS-CoV-2. The identification of factors which govern influenza virus multiplication and determine flu severity in humans is critical to develop effective and tailored antiviral strategies. We have discovered that acetylation, an epigenetic protein modification, plays a critical role in influenza virus infection and may determine the severity of flu in humans. Current research is focused on elucidating the role of acetylation machinery in influenza virus infection. We use human lung epithelial cells as the infection model of type A influenza virus. We manipulate the expression of genes which control acetylation by tools like RNA Interference and CRISPR/Cas9 and then analyse the effect on influenza virus replication and pathogenesis and host innate immune response by RNA Sequencing, mass spectrometry, plaque assay, qRT-PCR, and western blotting.
Publications
Nagesh, P. T., & Husain, M. (2016). Influenza A virus dysregulates host histone deacetylase 1 that inhibits viral infection in lung epithelial cells. Journal of Virology, 90(9), 4614-4625. doi: 10.1128/jvi.00126-16
Husain, M., & Cheung, C.-Y. (2014). Histone deacetylase 6 inhibits influenza A virus release by downregulating the trafficking of viral components to the plasma membrane via its substrate, acetylated microtubules. Journal of Virology, 88(19), 11229-11239. doi: 10.1128/jvi.00727-14
Chen, D.-Y., & Husain, M. (2016). Caspase-mediated degradation of host cortactin that promotes influenza A virus infection in epithelial cells. Virology, 497, 146-156. doi: 10.1016/j.virol.2016.07.017
Husain, M. (2014). Avian influenza A (H7N9) virus infection in humans: Epidemiology, evolution, and pathogenesis. Infection, Genetics & Evolution, 28, 304-312. doi: 10.1016/j.meegid.2014.10.016
Husain, M., & Harrod, K. S. (2011). Enhanced acetylation of alpha-tubulin in influenza A virus infected epithelial cells. FEBS Letters, 585(1), 128-132. doi: 10.1016/j.febslet.2010.11.023
Nagesh, P. T., & Husain, M. (2016). Influenza A virus dysregulates host histone deacetylase 1 that inhibits viral infection in lung epithelial cells. Journal of Virology, 90(9), 4614-4625. doi: 10.1128/jvi.00126-16
Journal - Research Article
Husain, M., & Cheung, C.-Y. (2014). Histone deacetylase 6 inhibits influenza A virus release by downregulating the trafficking of viral components to the plasma membrane via its substrate, acetylated microtubules. Journal of Virology, 88(19), 11229-11239. doi: 10.1128/jvi.00727-14
Journal - Research Article
Chen, D.-Y., & Husain, M. (2016). Caspase-mediated degradation of host cortactin that promotes influenza A virus infection in epithelial cells. Virology, 497, 146-156. doi: 10.1016/j.virol.2016.07.017
Journal - Research Article
Husain, M. (2014). Avian influenza A (H7N9) virus infection in humans: Epidemiology, evolution, and pathogenesis. Infection, Genetics & Evolution, 28, 304-312. doi: 10.1016/j.meegid.2014.10.016
Journal - Research Article
Husain, M., & Harrod, K. S. (2011). Enhanced acetylation of alpha-tubulin in influenza A virus infected epithelial cells. FEBS Letters, 585(1), 128-132. doi: 10.1016/j.febslet.2010.11.023
Journal - Research Article
Husain, M., & Harrod, K. S. (2009). Influenza A virus-induced caspase-3 cleaves the histone deacetylase 6 in infected epithelial cells. FEBS Letters, 583(15), 2517-2520. doi: 10.1016/j.febslet.2009.07.005
Journal - Research Article
Zaraket, H., Baranovich, T., Kaplan, B. S., Carter, R., Song, M.-S., Paulson, J. C., … Husain, M., … Webby, R. J. (2015). Mammalian adaptation of influenza A(H7N9) virus is limited by a narrow genetic bottleneck. Nature Communications, 6, 6553. doi: 10.1038/ncomms7553
Journal - Research Article
Singh, S. P., Gundavarapu, S., Smith, K. R., Chand, H. S., Saeed, A. I., Mishra, N. C., … Husain, M., … Sopori, M. L. (2013). Gestational exposure of mice to secondhand cigarette smoke causes bronchopulmonary dysplasia blocked by the nicotinic receptor antagonist mecamylamin. Environmental Health Perspectives, 121(8), 957-964. doi: 10.1289/ehp.1306611
Journal - Research Article
Husain, M., Weisberg, A. S., & Moss, B. (2007). Sequence-independent targeting of transmembrane proteins synthesized within vaccinia virus factories to nascent viral membranes. Journal of Virology, 81(6), 2646-2655. doi: 10.1128/JVI.02631-06
Journal - Research Article
Husain, M., Weisberg, A. S., & Moss, B. (2006). Existence of an operative pathway from the endoplasmic reticulum to the immature poxvirus membrane. PNAS, 103(51), 19506-19511. doi: 10.1073/pnas.0609406103
Journal - Research Article
Husain, M., Weisberg, A. S., & Moss, B. (2007). Resistance of a vaccinia virus A34R deletion mutant to spontaneous rupture of the outer membrane of progeny virions on the surface of infected cells. Virology, 366(2), 424-432. doi: 10.1016/j.virol.2007.05.015
Journal - Research Article
