Professor Debbie Hay
| Position | Professor |
|---|---|
| Department | Department of Pharmacology and Toxicology |
| Qualifications | BSc(Hons) PhD |
Research
After 18 years at the University of Auckland, Debbie moved to the University of Otago in 2020. Research in the Hay laboratory focuses on G protein-coupled receptors (GPCRs). These proteins are the cellular targets of natural hormones and are some of the most important drug targets. Her research aims to contribute to the development of medicines to treat migraine, among other conditions. Debbie is passionate about raising the profile of migraine in New Zealand and improving the lives of patients who have this condition.
Work in the lab spans a range of areas with local and international collaborators in academia and industry, including: pharmacology, medicinal chemistry, peptide chemistry, structural biology, molecular biology, immunohistochemistry and culture of cell lines and primary cells. A particular focus of the lab is class B GPCRs and their interactions with accessory proteins known as “receptor activity-modifying proteins”.
Debbie is a well-cited researcher (Web of Science, Highly Cited), who was elected Fellow of the British Pharmacological Society in 2018 (FBPhS). Other honours include the Gavin and Ann Kellaway Medical Research Fellowship for senior medical researchers (2018), an RSNZ James Cook Research Fellowship (2017), the prestigious 2015 Tregear Award (Australian Peptide Association), the 2013 Novartis Prize (British Pharmacological Society) and the 2013 Custom Science NZSBMB award.
She is an Editorial Board member of the British Journal of Pharmacology since 2015 (Editor Performance Award 2016), and Corresponding Member of the International Union of Basic and Clinical Pharmacology Committee on Receptor Nomenclature and Drug Classification (NC-IUPHAR), chairing its subcommittee on nomenclature of CGRP peptide family receptors. This committee provides influential educational resources for pharmacologists and non-pharmacologists, the pharmaceutical industry and academia.
Debbie is an active mentor of young researchers, is a British Pharmacological Society Women in Pharmacology mentor (2012-present), and has given many invited talks on mentoring, such as 'Mentors: what are they and how do I find one', 'Keep calm and write a paper', 'Keep calm and write a grant application', for early career researchers.
Publications
Fernando, S. J. A., Wang, Q., Hay, D. L., Bathgate, R. A. D., Shepherd, P. R., & Lee, K. L. (2023). Evidence that RXFP4 is located in enterochromaffin cells and can regulate production and release of serotonin. Bioscience Reports, 43(4), BSR20221956. doi: 10.1042/BSR20221956
Kotliar, I. B., Lorenzen, E., Schwenk, J. M., Hay, D. L., & Sakmar, T. P. (2023). Elucidating the interactome of G protein-coupled receptors and receptor activity-modifying proteins. Pharmacological Reviews, 75, 1-34. doi: 10.1124/pharmrev.120.000180
Rees, T. A., Hay, D. L., & Walker, C. S. (2023). Comment on Yoo et al. Amylin protein expression in the rat brain and neuro-2a cells. Int. J. Mol. Sci. 2022, 23, 4348. International Journal of Molecular Sciences, 24, 1058. doi: 10.3390/ijms24021058
Russo, A. F., & Hay, D. L. (2023). CGRP physiology, pharmacology, and therapeutic targets: Migraine and beyond. Physiological Reviews, 103, 1565-1644. doi: 10.1152/physrev.00059.2021
Hendrikse, E. R., Rees, T. A., Tasma, Z., Garelja, M. L., Siow, A., Harris, P. W. R., … Hay, D. L. (2022). Characterization of antibodies against receptor activity-modifying protein 1 (RAMP1): A cautionary tale. International Journal of Molecular Sciences, 23, 16035. doi: 10.3390/ijms232416035
Hay, D. L., & Dickerson, I. M. (Eds.). (2010). The calcitonin gene-related peptide family: Form, function and future perspectives. Dordrecht, The Netherlands: Springer, 236p. doi: 10.1007/978-90-481-2909-6
Edited Book - Research
Chuang, C.-L., & Hay, D. L. (2020). Amylin. In S. Offermanns & W. Rosenthal (Eds.), Encyclopaedia of molecular pharmacology. Cham, Switzerland: Springer. doi: 10.1007/978-3-030-21573-6_10021-1
Chapter in Book - Research
Pioszak, A. A., & Hay, D. L. (2020). RAMPs as allosteric modulators of the calcitonin and calcitonin-like class B G protein-coupled receptors. In C. J. Langmead (Ed.), From structure to clinical development: Allosteric modulation of G protein-coupled receptors. (pp. 115-141). Cambridge, MA: Academic Press. doi: 10.1016/bs.apha.2020.01.001
Chapter in Book - Research
Hay, D. L. (2019). CGRP receptor biology: Is there more than one receptor? In S. Brain & P. Geppetti (Eds.), Calcitonin gene-related peptide (CGRP) mechanisms: Focus on migraine. (pp. 13-22). Cham, Switzerland: Springer. doi: 10.1007/164_2018_131
Chapter in Book - Research
Gingell, J. J., Walker, C. S., & Hay, D. L. (2017). Class B GPCR: Receptors and RAMPs. In K. Herrick-Davis, G. Milligan & G. Di Giovanni (Eds.), G-protein-coupled receptor dimers. (pp. 289-305). Cham, Switzerland: Humana Press. doi: 10.1007/978-3-319-60174-8_11
Chapter in Book - Research
Hay, D. L., Conner, A. C., & Poyner, D. R. (2013). Calcitonin gene-related peptide and adrenomedullin receptors. In M. D. Lane & W. J. Lennarz (Eds.), Encyclopedia of biological chemistry. (2nd ed.) (pp. 265-269). London, UK: Academic Press. doi: 10.1016/B978-0-12-378630-2.00363-7
Chapter in Book - Research
Barwell, J., Wootten, D., Simms, J., Hay, D. L., & Poyner, D. R. (2012). RAMPs and CGRP receptors. In W. S. Spielman & N. Parameswaran (Eds.), RAMPs. (pp. 13-24). Austin, TX: Landes Bioscience. doi: 10.1007/978-1-4614-2364-5_2
Chapter in Book - Research
Sexton, P. M., Poyner, D. R., Simms, J., Christopoulos, A., & Hay, D. L. (2012). RAMPs as drug targets. In W. S. Spielman & N. Parameswaran (Eds.), RAMPs. (pp. 61-74). Austin, TX: Landes Bioscience. doi: 10.1007/978-1-4614-2364-5_6
Chapter in Book - Research
Barwell, J., Simms, J., Conner, A., Hay, D., Wheatley, M., & Poyner, D. (2010). Ligand binding and activation of the CGRP receptor. In D. L. Hay & I. M. Dickerson (Eds.), The calcitonin gene-related peptide family: Form, function and future perspectives. (pp. 23-40). Dordrecht, The Netherlands: Springer. doi: 10.1007/978-90-481-2909-6_2
Chapter in Book - Research
Hay, D. L. (2010). Conclusions and future perspectives. In D. L. Hay & I. M. Dickerson (Eds.), The calcitonin gene-related peptide family: Form, function and future perspectives. (pp. 235-236). Dordrecht, The Netherlands: Springer. doi: 10.1007/978-90-481-2909-6_14
Chapter in Book - Research
Wootten, D. L., Simms, J., Hay, D. L., Christopoulos, A., & Sexton, P. M. (2010). Receptor activity modifying proteins and their potential as drug targets. In C. A. Lunn (Ed.), Membrane proteins as drug targets. (pp. 53-79). London, UK: Academic Press. doi: 10.1016/S1877-1173(10)91003-X
Chapter in Book - Research
Hay, D. (2008). Amylin receptor. In S. J. Enna & D. B. Byland (Eds.), xPharm: The comprehensive pharmacology reference. (Online ed.) Elsevier. doi: 10.1016/B978-008055232-3.60178-3
Chapter in Book - Research
Hay, D. (2008). Calcitonin gene-related peptide receptor. In S. J. Enna & D. B. Byland (Eds.), xPharm: The comprehensive pharmacology reference. (Online ed.) Elsevier. doi: 10.1016/B978-008055232-3.60177-1
Chapter in Book - Research
Hay, D. (2008). Calcitonin receptor. In S. J. Enna & D. B. Byland (Eds.), xPharm: The comprehensive pharmacology reference. (Online ed.) Elsevier. doi: 10.1016/B978-008055232-3.60176-X
Chapter in Book - Research
Hay, D. (2008). Calcitonin-type receptor. In S. J. Enna & D. B. Byland (Eds.), xPharm: The comprehensive pharmacology reference. (Online ed.) Elsevier. doi: 10.1016/B978-008055232-3.60175-8
Chapter in Book - Research
Fernando, S. J. A., Wang, Q., Hay, D. L., Bathgate, R. A. D., Shepherd, P. R., & Lee, K. L. (2023). Evidence that RXFP4 is located in enterochromaffin cells and can regulate production and release of serotonin. Bioscience Reports, 43(4), BSR20221956. doi: 10.1042/BSR20221956
Journal - Research Article
Kotliar, I. B., Lorenzen, E., Schwenk, J. M., Hay, D. L., & Sakmar, T. P. (2023). Elucidating the interactome of G protein-coupled receptors and receptor activity-modifying proteins. Pharmacological Reviews, 75, 1-34. doi: 10.1124/pharmrev.120.000180
Journal - Research Article
Russo, A. F., & Hay, D. L. (2023). CGRP physiology, pharmacology, and therapeutic targets: Migraine and beyond. Physiological Reviews, 103, 1565-1644. doi: 10.1152/physrev.00059.2021
Journal - Research Article
Alexander, T. I., Tasma, Z., Siow, A., Rees, T. A., Brimble, M. A., Harris, P. W. R., Hay, D. L., & Walker, C. S. (2022). Novel fluorescently labeled PACAP and VIP highlight differences between peptide internalization and receptor pharmacology. ACS Pharmacology & Translational Science. Advance online publication. doi: 10.1021/acsptsci.2c00124
Journal - Research Article
Boer, G. A., Hay, D. L., & Tups, A. (2022). Obesity pharmacotherapy: Incretin action in the central nervous system [Review]. Trends in Pharmacological Sciences, 44(1), 50-63. doi: 10.1016/j.tips.2022.11.001
Journal - Research Article
Cao, J., Belousoff, M. J., Liang, Y.-L., Johnson, R. M., Josephs, T. M., Fletcher, M. M., … Hay, D. L., … Sexton, P. M. (2022). A structural basis for amylin receptor phenotype. Science, 375, eabm9609. doi: 10.1126/science.abm9609
Journal - Research Article
Garelja, M. L., & Hay, D. L. (2022). A narrative review of the calcitonin peptide family and associated receptors as migraine targets: Calcitonin gene-related peptide and beyond. Headache, 62(9), 1093-1104. doi: 10.1111/head.14388
Journal - Research Article
Garelja, M. L., Bower, R. L., Brimble, M. A., Chand, S., Harris, P. W. R., Jamaluddin, M. A., … Hay, D. L. (2022). Pharmacological characterisation of mouse calcitonin and calcitonin receptor-like receptors reveals differences compared to human receptors. British Journal of Pharmacology, 179, 416-434. doi: 10.1111/bph.15628
Journal - Research Article
Hendrikse, E. R., Rees, T. A., Tasma, Z., Garelja, M. L., Siow, A., Harris, P. W. R., … Hay, D. L. (2022). Characterization of antibodies against receptor activity-modifying protein 1 (RAMP1): A cautionary tale. International Journal of Molecular Sciences, 23, 16035. doi: 10.3390/ijms232416035
Journal - Research Article
Hendrikse, E. R., Rees, T. A., Tasma, Z., Le Foll, C., Lutz, T. A., Siow, A., … Hay, D. L. (2022). Calcitonin receptor antibody validation and expression in the rodent brain. Cephalagia. Advance online publication. doi: 10.1177/03331024221084029
Journal - Research Article
Jamaluddin, A., Chuang, C.-L., Williams, E. T., Siow, A., Yang, S. H., Harris, P. W. R., … Hay, D. L., & Loomes, K. M. (2022). Lipidated calcitonin gene-related peptide (CGRP) peptide antagonists retain CGRP receptor activity and attenuate CGRP action in vivo. Frontiers in Pharmacology, 13, 832589. doi: 10.3389/fphar.2022.832589
Journal - Research Article
Kobayashi, A., Hamada, M., Yoshida, M.-a., Kobayashi, Y., Tsutsui, N., Sekiguchi, T., … Hay, D. L., … Sakamoto, H. (2022). Vasopressin-oxytocin-type signaling is ancient and has a conserved water homeostasis role in euryhaline marine planarians. Science Advances, 8, eabk0331. doi: 10.1126/sciadv.abk0331
Journal - Research Article
Rees, T. A., Hendrikse, E. R., Hay, D. L., & Walker, C. S. (2022). Beyond CGRP: The calcitonin peptide family as targets for migraine and pain. British Journal of Pharmacology, 179, 381-399. doi: 10.1111/bph.15605
Journal - Research Article
Rees, T. A., Russo, A. F., O'Carroll, S. J., Hay, D. L., & Walker, C. S. (2022). CGRP and the calcitonin receptor are co-expressed in mouse, rat and human trigeminal ganglia neurons. Frontiers in Physiology, 13, 860037. doi: 10.3389/fphys.2022.860037
Journal - Research Article
Tasma, Z., Siow, A., Harris, P. W. R., Brimble, M. A., Hay, D. L., & Walker, C. S. (2022). Characterisation of agonist signalling profiles and agonist-dependent antagonism at PACAP-responsive receptors: Implications for drug discovery. British Journal of Pharmacology, 179, 435-453. doi: 10.1111/bph.15700
Journal - Research Article
Tasma, Z., Siow, A., Harris, P. W. R., Brimble, M. A., O'Carroll, S. J., Hay, D. L., & Walker, C. S. (2022). PAC1, VPAC1, and VPAC2 receptor expression in rat and human trigeminal ganglia: Characterization of PACAP-responsive receptor antibodies. International Journal of Molecular Sciences, 23, 13797. doi: 10.3390/ijms232213797
Journal - Research Article
Alexander, S. P. H., Christopoulos, A., Davenport, A. P., Kelly, E., Mathie, A., Peters, J. A., … Hay, D. L., … Ye, R. D. (2021). The concise guide to Pharmacology 2021/22: G protein-coupled receptors. British Journal of Pharmacology, 178(Suppl. 1), S27-S156. doi: 10.1111/bph.15538
Journal - Research Article
Ashina, M., Terwindt, G. M., Al-Karagholi, M. A.-M., de Boer, I., Lee, M. J., Hay, D. L., … Goadsby, P. J. (2021). Migraine: Disease characterisation, biomarkers, and precision medicine. Lancet, 397, 1496-1504. doi: 10.1016/s0140-6736(20)32162-0
Journal - Research Article
Ghanizada, H., Al-Karagholi, M. A.-M., Arngrim, N., Mørch-Rasmussen, M., Walker, C. S., Hay, D. L., & Ashina, M. (2021). Effect of adrenomedullin on migraine-like attacks in patients with migraine: A randomized crossover study. Neurology, 96(20), e2488-e2499. doi: 10.1212/wnl.0000000000011930
Journal - Research Article
Ghanizada, H., Al-Karagholi, M. A.-M., Walker, C. S., Arngrim, N., Rees, T., Petersen, J., … Hay, D. L., & Ashina, M. (2021). Amylin analog pramlintide induces migraine-like attacks in patients. Annals of Neurology, 89(6), 1157-1171. doi: 10.1002/ana.26072
Journal - Research Article
Josephs, T. M., Belousoff, M. J., Liang, Y.-L., Piper, S. J., Cao, J., Garama, D. J., … Hay, D. L., … Sexton, P. M. (2021). Structure and dynamics of the CGRP receptor in apo and peptide-bound forms. Science, 372, eabf7258. doi: 10.1126/science.abf7258
Journal - Research Article
Garelja, M. L., Au, M., Brimble, M. A., Gingell, J. J., Hendrikse, E. R., Lovell, A., … Hay, D. L. (2020). Molecular mechanisms of class B GPCR activation: Insights from adrenomedullin receptors. ACS Pharmacology & Translational Science, 3(2), 246-262. doi: 10.1021/acsptsci.9b00083
Journal - Research Article
Gingell, J. J., Rees, T. A., Hendrikse, E. R., Siow, A., Rennison, D., Scotter, J., … Hay, D. L. (2020). Distinct patterns of internalization of different calcitonin gene-related peptide receptors. ACS Pharmacology & Translational Science, 3(2), 296-304. doi: 10.1021/acsptsci.9b00089
Journal - Research Article
Hendrikse, E. R., Liew, L. P., Bower, R. L., Bonnet, M., Jamaluddin, M. A., Prodan, N., … Hay, D. L. (2020). Identification of small-molecule positive modulators of calcitonin-like receptor-based receptors. ACS Pharmacology & Translational Science, 3(2), 305-320. doi: 10.1021/acsptsci.9b00108
Journal - Research Article
Liang, Y.-L., Belousoff, M. J., Fletcher, M. M., Zhang, X., Khoshouei, M., Deganutti, G., … Hay, D. L., … Sexton, P. M. (2020). Structure and dynamics of adrenomedullin receptors AM1 and AM2 reveal key mechanisms in the control of receptor phenotype by receptor activity-modifying proteins. ACS Pharmacology & Translational Science, 3(2), 263-284. doi: 10.1021/acsptsci.9b00080
Journal - Research Article
Lu, B. L., Loomes, K. M., Hay, D. L., Harris, P. W. R., & Brimble, M. A. (2020). Synthesis of isotopically labelled αCGRP8-37 and its lipidated analogue. Journal of Labelled Compounds & Radiopharmaceuticals, 63, 325-332. doi: 10.1002/jlcr.3838
Journal - Research Article
Noh, D., Bower, R. L., Hay, D. L., Zhyvoloup, A., & Raleigh, D. P. (2020). Analysis of amylin consensus sequences suggests that human amylin is not optimized to minimize amyloid formation and provides clues to factors that modulate amyloidogenicity. ACS Chemical Biology, 15(6), 1408-1416. doi: 10.1021/acschembio.9b01050
Journal - Research Article
Pan, K. S., Siow, A., Hay, D. L., & Walker, C. S. (2020). Antagonism of CGRP signaling by rimegepant at two receptors. Frontiers in Pharmacology, 11, 1240. doi: 10.3389/fphar.2020.01240
Journal - Research Article
Sowers, L. P., Wang, M., Rea, B. J., Taugher, R. J., Kuburas, A., Kim, Y., … Hay, D. L., & Russo, A. F. (2020). Stimulation of posterior thalamic nuclei induces photophobic behavior in mice. Headache, 60, 1961-1981. doi: 10.1111/head.13917
Journal - Research Article
Tasma, Z., Wills, P., Hay, D. L., & Walker, C. S. (2020). Agonist bias and agonist-dependent antagonism at corticotrophin releasing factor receptors. Pharmacology Research & Perspectives, 8(3), e00595. doi: 10.1002/prp2.595
Journal - Research Article
Alexander, S. P. H., Christopoulos, A., Davenport, A. P., Kelly, E., Mathie, A., Peters, J. A., … and CGTP Collaborators, including Hay, D. (2019). The concise guide to pharmacology 2019/2020. British Journal of Pharmacology, 176(Suppl. 1), S1-S493. doi: 10.1111/bph.14746
Journal - Research Article
Musa, H., Hendrikse, E. R., Brimble, M. A., Garelja, M. L., Watkins, H. A., Harris, P. W. R., & Hay, D. L. (2019). Pharmacological characterization and investigation of N-terminal loop amino acids of adrenomedullin 2 that are important for receptor activation. Biochemistry, 58(32), 3468-3474. doi: 10.1021/acs.biochem.9b00571
Journal - Research Article
Yule, L. R., Garelja, M. L., Hendrikse, E. R., Gingell, J. J., Poyner, D. R., Harris, P. W. H., Brimble, M. A., & Hay, D. L. (2019). A potent fluorescent calcitonin gene-related peptide analogue enables visualization of receptor internalization. Peptide Science, 111(6), e2416. doi: 10.1002/pep2.24126
Journal - Research Article
Akter, R., Bower, R. L., Abedini, A., Schmidt, A. M., Hay, D. L., & Raleigh, D. P. (2018). Amyloidogenicity, cytotoxicity and receptor activity of bovine amylin: Implications for xenobiotic transplantation and the design of non-toxic amylin variants. ACS Chemical Biology, 13, 2747-2757. doi: 10.1021/acschembio.8b00690
Journal - Research Article
Booe, J. M., Warner, M. L., Roehrkasse, A. M., Hay, D. L., & Pioszak, A. A. (2018). Probing the mechanism of receptor activity-modifying protein modulation of GPCR ligand selectivity through rational design of potent adrenomedullin and calcitonin gene-related peptide antagonists. Molecular Pharmacology, 93(4), 355-367. doi: 10.1124/mol.117.110916
Journal - Research Article
Dal Maso, E., Zhu, Y., Pham, V., Reynolds, C. A., Deganutti, G., Hick, C. A., … Hay, D. L., … Wootten, D. (2018). Extracellular loops 2 and 3 of the calcitonin receptor selectively modify agonist binding and efficacy. Biochemical Pharmacology, 150, 214-244. doi: 10.1016/j.bcp.2018.02.005
Journal - Research Article
Garelja, M. L., Walker, C. A., Siow, A., Yang, S. H., Harris, P. W. R., Brimble, M. A., … Hay, D. L. (2018). Receptor activity modifying proteins have limited effects on the class B G protein-coupled receptor calcitonin receptor-like receptor stalk. Biochemistry, 57(8), 1410-1422. doi: 10.1021/acs.biochem.7b01180
Journal - Research Article
Liang, Y.-L., Khoshouei, M., Deganutti, G., Glukhova, A., Koole, C., Peat, T. S., … Hay, D. L., … Sexton, P. M. (2018). Cryo-EM structure of the active, Gs protein complexed, human CGRP receptor. Nature, 561(7724), 492-497. doi: 10.1038/s41586-018-0535-y
Journal - Research Article
Simms, J., Uddin, R., Sakmar, T. P., Gingell, J. J., Garelja, M. L., Hay, D. L., … Poyner, D. R. (2018). Photoaffinity cross-linking and unnatural amino acid mutagenesis reveal insights into calcitonin gene-related peptide binding to the CalcitoninRreceptor-Like Receptor/Receptor Activity-Modifying Protein 1 (CLR/RAMP1) complex. Biochemistry, 57(32), 4915-4922. doi: 10.1021/acs.biochem.8b00502
Journal - Research Article
Walker, C. S., Raddant, A. C., Woolley, M. J., Russo, A. F., & Hay, D. L. (2018). CGRP receptor antagonist activity of olcegepant depends on the signalling pathway measured. Cephalagia, 38(3), 437-451. doi: 10.1177/0333102417691762
Journal - Research Article
Williams, E. T., Harris, P. W. R., Jamaluddin, M. A., Loomes, K. M., Hay, D. L., & Brimble, M. A. (2018). Solid-phase thiol–ene lipidation of peptides for the synthesis of a potent CGRP receptor antagonist. Angewandte Chemie International Edition, 57(36), 11640-11643. doi: 10.1002/anie.201805208
Journal - Research Article
Alexander, S. P. H., Kelly, E., Marrion, N. V., Peters, J. A., Faccenda, E., Harding, S. D., … and CGTP Collaborators, including Hay, D. L. (2017). The concise guide to pharmacology 2017/18. British Journal of Pharmacology, 174(Suppl. 1), S1-S446. doi: 10.1111/bph.13987
Journal - Research Article
Lee, S.-M., Booe, J. M., Gingell, J. J., Sjoelund, V., Hay, D. L., & Pioszak, A. A. (2017). N-glycosylation of asparagine 130 in the extracellular domain of the human calcitonin receptor significantly increases peptide hormone affinity. Biochemistry, 56(26), 3380-3393. doi: 10.1021/acs.biochem.7b00256
Journal - Research Article
Woolley, M. J., Reynolds, C. A., Simms, J., Walker, C. S., Mobarec, J. C., Garelja, M. L., … Hay, D. L. (2017). Receptor activity-modifying protein dependent and independent activation mechanisms in the coupling of calcitonin gene-related peptide and adrenomedullin receptors to Gs. Biochemical Pharmacology, 142, 96-110. doi: 10.1016/j.bcp.2017.07.005
Journal - Research Article
Bower, R. L., & Hay, D. L. (2016). Amylin structure-function relationships and receptor pharmacology: Implications for amylin mimetic drug development. British Journal of Pharmacology, 173(12), 1883-1898. doi: 10.1111/bph.13496
Journal - Research Article
Bower, R. L., Eftekhari, S., Waldvogel, H. J., Faull, R. L. M., Tajti, J., Edvinsson, L., Hay, D. L., & Walker, C. S. (2016). Mapping the calcitonin receptor in human brain stem. American Journal of Physiology: Regulatory, Integrative & Comparative Physiology, 310(9), R788-R793. doi: 10.1152/ajpregu.00539.2015
Journal - Research Article
Gingell, J. J., Simms, J., Barwell, J., Poyner, D. R., Watkins, H. A., Pioszak, A. A., … Hay, D. L. (2016). An allosteric role for receptor activity-modifying proteins in defining GPCR pharmacology. Cell Discovery, 2, 16012. doi: 10.1038/celldisc.2016.12
Journal - Research Article
Lee, S.-M., Hay, D. L., & Pioszak, A. A. (2016). Calcitonin and amylin receptor peptide interaction mechanisms: Insights into peptide-binding modes and allosteric modulation of the calcitonin receptor by receptor activity-modifying proteins. Journal of Biological Chemistry, 291(16), 8686-8700. doi: 10.1074/jbc.A115.713628
Journal - Research Article
Watkins, H. A., Chakravarthy, M., Abhayawardana, R. S., Gingell, J. J., Garelja, M. L., Pardamwar, M., … Hay, D. L. (2016). Receptor activity-modifying proteins 2 and 3 generate adrenomedullin receptor subtypes with distinct molecular properties. Journal of Biological Chemistry, 291(22), 11657-11675. doi: 10.1074/jbc.M115.688218
Journal - Research Article
Yule, L. R., Bower, R. L., Kaur, H., Kowalczyk, R., Hay, D. L., & Brimble, M. A. (2016). Synthesis and amylin receptor activity of glycomimetics of pramlintide using click chemistry. Organic & Biomolecular Chemistry, 14(23), 5238-5245. doi: 10.1039/c6ob00850j
Journal - Research Article
Alexander, S. P. H., Kelly, E., Marrion, N., Peters, J. A., Benson, H. E., Faccenda, E., … and CGTP Collaborators, including Hay, D. L. (2015). The concise guide to Pharmacology 2015/16: Overview. British Journal of Pharmacology, 172(24), 5729-6202. doi: 10.1111/bph.12931
Journal - Research Article
Booe, J. M., Walker, C. S., Barwell, J., Kuteyi, G., Simms, J., Jamaluddin, M. A., … Hay, D. L. (2015). Structural basis for receptor activity-modifying protein-dependent selective peptide recognition by a G protein-coupled receptor. Molecular Cell, 58(6), 1040-1052. doi: 10.1016/j.molcel.2015.04.018
Journal - Research Article
Kim, J. S., Brownjohn, P. W., Dyer, B. S., Beltramo, M., Walker, C. S., Hay, D. L., … Tyndall, J. D. A., & Anderson, G. M. (2015). Anxiogenic and stressor effects of the hypothalamic neuropeptide RFRP-3 are overcome by the NPFFR antagonist GJ14. Endocrinology, 156(11), 4152-4162. doi: 10.1210/en.2015-1532
Journal - Research Article
Walker, C. S., Eftekhari, S., Bower, R. L., Wilderman, A., Insel, P. A., Edvinsson, L., … Hay, D. L. (2015). A second trigeminal CGRP receptor: Function and expression of the AMY1 receptor. Annals of Clinical & Translational Neurology, 2(6), 595-608. doi: 10.1002/acn3.197
Journal - Research Article
Whiting, L., Stewart, K. W., Hay, D. L., Harris, P. W., Choong, Y. S., Phillips, A. R. J., … Cooper, G. J. S. (2015). Glicentin-related pancreatic polypeptide inhibits glucose-stimulated insulin secretion from the isolated pancreas of adult male rats. Physiological Reports, 3(12), e12638. doi: 10.14814/phy2.12638
Journal - Research Article
Gingell, J. J., Burns, E. R., & Hay, D. L. (2014). Activity of pramlintide, rat and human amylin but not Aβ1-42 at human amylin receptors. Endocrinology, 155(1), 21-26. doi: 10.1210/en.2013-1658
Journal - Research Article
Hay, D. L., Harris, P. W. R., Kowalczyk, R., Brimble, M. A., Rathbone, D. L., Barwell, J., … Poyner, D. R. (2014). Structure-activity relationships of the N-terminus of calcitonin gene-related peptide: Key roles of alanine-5 and threonine-6 in receptor activation. British Journal of Pharmacology, 171(2), 415-426. doi: 10.1111/bph.12464
Journal - Research Article
Kowalczyk, R., Brimble, M. A., Tomabechi, Y., Fairbanks, A. J., Fletcher, M., & Hay, D. L. (2014). Convergent chemoenzymatic synthesis of a library of glycosylated analogues of pramlintide: Structure-activity relationships for amylin receptor agonism. Organic & Biomolecular Chemistry, 12(41), 8142-8151. doi: 10.1039/c4ob01208a
Journal - Research Article
Walker, C. S., Hay, D. L., Fitzpatrick, S. M., Cooper, G. J. S., & Loomes, K. M. (2014). α-Calcitonin gene related peptide (α-CGRP) mediated lipid mobilization in 3T3-L1 adipocytes. Peptides, 58, 14-19. doi: 10.1016/j.peptides.2014.05.011
Journal - Research Article
Walker, C. S., Sundrum, T., & Hay, D. L. (2014). PACAP receptor pharmacology and agonist bias: Analysis in primary neurons and glia from the trigeminal ganglia and transfected cells. British Journal of Pharmacology, 171(6), 1521-1533. doi: 10.1111/bph.12541
Journal - Research Article
Watkins, H. A., Walker, C. S., Ly, K. N., Bailey, R. J., Barwell, J., Poyner, D. R., & Hay, D. L. (2014). Receptor activity-modifying protein-dependent effects of mutations in the calcitonin receptor-like receptor: Implications for adrenomedullin and calcitonin gene-related peptide pharmacology. British Journal of Pharmacology, 171(3), 772-788. doi: 10.1111/bph.12508
Journal - Research Article
Cognard, E., Dargaville, C. G., Hay, D. L., & Shepherd, P. R. (2013). Identification of a pathway by which glucose regulates β-catenin signalling via the cAMP/protein kinase A pathway in β-cell models. Biochemical Journal, 449(3), 803-811. doi: 10.1042/BJ20121454
Journal - Research Article
Harris, P. W. R., Kowalczyk, R., Hay, D. L., & Brimble, M. A. (2013). A single pseudoproline and microwave solid phase peptide synthesis facilitates an efficient synthesis of human amylin 1-37. International Journal of Peptide Research & Therapeutics, 19(2), 147-155. doi: 10.1007/s10989-012-9325-9
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Kuwasako, K., Hay, D. L., Nagata, S., Murakami, M., Kitamura, K., & Kato, J. (2013). Functions of third extracellular loop and helix 8 of family B GPCRs complexed with RAMPs and characteristics of their receptor trafficking. Current Protein & Peptide Science, 14(5), 416-428. doi: 10.2174/13892037113149990058
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Qi, T., Dong, M., Wootten, D., Miller, L. J., & Hay, D. L. (2013). Receptor activity-modifying protein-dependent impairment of calcitonin receptor splice variant Δ(1-47)hCT(a) function. British Journal of Pharmacology, 168(3), 644-657. doi: 10.1111/j.1476-5381.2012.02197.x
Journal - Research Article
Vohra, S., Taddese, B., Conner, A. C., Poyner, D. R., Hay, D. L., Barwell, J., … Reynolds, C. A. (2013). Similarity between class A and class B G-protein-coupled receptors exemplified through calcitonin gene-related peptide receptor modelling and mutagenesis studies. Journal of the Royal Society Interface, 10(79), 20120846. doi: 10.1098/rsif.2012.0846
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Watkins, H. A., Au, M., Bobby, R., Archbold, J. K., Abdul-Manan, N., Moore, J. M., … Hay, D. L. (2013). Identification of key residues involved in adrenomedullin binding to the AM1 receptor. British Journal of Pharmacology, 169(1), 143-155. doi: 10.1111/bph.12118
Journal - Research Article
Woolley, M. J., Watkins, H. A., Taddese, B., Karakullukcu, Z. G., Barwell, J., Smith, K. J., Hay, D. L., … Conner, A. C. (2013). The role of ECL2 in CGRP receptor activation: A combined modelling and experimental approach. Journal of the Royal Society Interface, 10(88), 20130589. doi: 10.1098/rsif.2013.0589
Journal - Research Article
Bailey, R. J., Walker, C. S., Ferner, A. H., Loomes, K. M., Prijic, G., Halim, A., … Hay, D. L. (2012). Pharmacological characterization of rat amylin receptors: Implications for the identification of amylin receptor subtypes. British Journal of Pharmacology, 166(1), 151-167. doi: 10.1111/j.1476-5381.2011.01717.x
Journal - Research Article
Halim, A., & Hay, D. L. (2012). The role of glutamic acid 73 in adrenomedullin interactions with rodent AM2 receptors. Peptides, 36(1), 137-141. doi: 10.1016/j.peptides.2012.04.011
Journal - Research Article
Kuwasako, K., Hay, D. L., Nagata, S., Hikosaka, T., Kitamura, K., & Kato, J. (2012). The third extracellular loop of the human calcitonin receptor-like receptor is crucial for the activation of adrenomedullin signalling. British Journal of Pharmacology, 166(1), 137-150. doi: 10.1111/j.1476-5381.2011.01803.x
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Qi, T., Ly, K., Poyner, D. R., Christopoulos, G., Sexton, P. M., & Hay, D. L. (2011). Structure-function analysis of amino acid 74 of human RAMP1 and RAMP3 and its role in peptide interactions with adrenomedullin and calcitonin gene-related peptide receptors. Peptides, 32(5), 1060-1067. doi: 10.1016/j.peptides.2011.03.004
Journal - Research Article
Bailey, R. J., Bradley, J. W. I., Poyner, D. R., Rathbone, D. L., & Hay, D. L. (2010). Functional characterization of two human receptor activity-modifying protein 3 variants. Peptides, 31(4), 579-584. doi: 10.1016/j.peptides.2009.12.016
Journal - Research Article
Gingell, J. J., Qi, T., Bailey, R. J., & Hay, D. L. (2010). A key role for tryptophan 84 in receptor activity-modifying protein 1 in the amylin 1 receptor. Peptides, 31(7), 1400-1404. doi: 10.1016/j.peptides.2010.03.027
Journal - Research Article
Moore, E. L., Gingell, J. J., Kane, S. A., Hay, D. L., & Salvatore, C. A. (2010). Mapping the CGRP receptor ligand binding domain: Tryptophan-84 of RAMP1 is critical for agonist and antagonist binding. Biochemical & Biophysical Research Communications, 394(1), 141-145. doi: 10.1016/j.bbrc.2010.02.131
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Qi, T., Simms, J., Bailey, R. J., Wheatley, M., Rathbone, D. L., Hay, D. L., & Poyner, D. R. (2010). Structure—function analysis of RAMP1 - RAMP3 chimeras. Biochemistry, 49(3), 522-531. doi: 10.1021/bi9019093
Journal - Research Article
Walker, C. S., Li, X., Whiting, L., Glyn-Jones, S., Zhang, S., Hickey, A. J., … Hay, D. L., … Loomes, K. M. (2010). Mice lacking the neuropeptide α-calcitonin gene-related peptide are protected against diet-induced obesity. Endocrinology, 151(9), 4257-4269. doi: 10.1210/en.2010-0284
Journal - Research Article
Robinson, S. D., Aitken, J. F., Bailey, R. J., Poyner, D. R., & Hay, D. L. (2009). Novel peptide antagonists of adrenomedullin and calcitonin gene-related peptide receptors: Identification, pharmacological characterization, and interactions with position 74 in receptor activity-modifying protein 1/3. Journal of Pharmacology & Experimental Therapeutics, 331(2), 513-521. doi: 10.1124/jpet.109.156448
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Simms, J., Hay, D. L., Bailey, R. J., Konycheva, G., Bailey, G., Wheatley, M., & Poyner, D. R. (2009). Structure—function analysis of RAMP1 by alanine mutagenesis. Biochemistry, 48(1), 198-205. doi: 10.1021/bi801869n
Journal - Research Article
Danaher, R. N., Loomes, K. M., Leonard, B. L., Whiting, L., Hay, D. L., Xu, L. Y., … Cooper, G. J. S. (2008). Evidence that α-calcitonin gene-related peptide is a neurohormone that controls systemic lipid availability and utilization. Endocrinology, 149(1), 154-160. doi: 10.1210/en.2007-0583
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Qi, T., Christopoulos, G., Bailey, R. J., Christopoulos, A., Sexton, P. M., & Hay, D. L. (2008). Identification of N-terminal receptor activity-modifying protein residues important for calcitonin gene-related peptide, adrenomedullin, and amylin receptor function. Molecular Pharmacology, 74(4), 1059-1071. doi: 10.1124/mol.108.047142
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Bailey, R. J., & Hay, D. L. (2007). Agonist-dependent consequences of proline to alanine substitution in the transmembrane helices of the calcitonin receptor. British Journal of Pharmacology, 151(5), 678-687. doi: 10.1038/sj.bjp.0707246
Journal - Research Article
Bailey, R. J., & Hay, D. L. (2006). Pharmacology of the human CGRP1 receptor in Cos 7 cells. Peptides, 27(6), 1367-1375. doi: 10.1016/j.peptides.2005.11.014
Journal - Research Article
Hay, D. L., Christopoulos, G., Christopoulos, A., & Sexton, P. M. (2006). Determinants of 1-piperidinecarboxamide, N-[2-[[5-amino-/-[[4-(4-pyridinyl) -/-piperazinyl]carbonyl]pentyl]amino]-1-[(3,5-dibromo-4-hydroxyphenyl)methyl] -2-oxoethyl]-4-(1,4-dihydro-2-oxo-3(2H)-quinazolinyl) (BIBN4096BS) affinity for calcitonin gene-related peptide and amylin receptors: The role of receptor activity modifying protein 1. Molecular Pharmacology, 70(6), 1984-1991. doi: 10.1124/mol.106.027953
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Hay, D. L., Poyner, D. R., & Sexton, P. M. (2006). GPCR modulation by RAMPs. Pharmacology & Therapeutics, 109(1-2), 173-197. doi: 10.1016/j.pharmthera.2005.06.015
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