Associate Professor Craig Marshall

Position	Associate Professor
Department	Department of Biochemistry
Qualifications	BSc (Hons) PhD (Otago)
Research summary	Biochemistry, physiology and ecology of cold adaptation

Research

Our major goal in this work is to improve our understanding of the structural and functional adaptations of the enzymes that fit them for function in Antarctic fish and how these adaptations compare with those of related fish from more temperate waters. In the long-term we plan to examine a number of proteins so that we can produce a general picture of how low temperature affects protein structure and function. This work will also deepen our understanding of the mechanisms by which ligands interact with enzymes and will improve our knowledge of how proteins fold.

In addition to the general question of how proteins fold to allow activity at low temperatures, we are interested in the specific question of the nature of the structure of antifreeze glycopeptides. We wish to answer this question by growing crystals of these proteins and solving the structure by X-ray crystallography.

Publications

Morgan-Richards, M., Marshall, C. J., Biggs, P. J., & Trewick, S. A. (2023). Insect freeze-tolerance Downunder: The microbial connection. Insects, 14(1), 89. doi: 10.3390/insects14010089

Wilson, P. W., Marshall, C., Bayer-Giraldi, M., & Mateo, M. A. (2020). Evidence for transition of metastable ice to hexagonal ice in bulk solutions at relatively high temperatures. International Journal of Geosciences, 11(8), 493-500. doi: 10.4236/ijg.2020.118025

Zawierucha, K., Marshall, C. J., Wharton, D., & Janko, K. (2019). A nematode in the mist: Scottnema lindsayae is the only soil metazoan in remote Antarctic deserts, at greater densities with altitude. Polar Research, 38, 1-12. doi: 10.33265/polar.v38.3494

Tate, W. P., & Marshall, C. J. (2018). Post-dormancy transcription and translation in the brine shrimp. In R. A. Browne, P. Sorgeloos & C. N. A. Trotman (Eds.), Artemia biology. (pp. 21-36). Boca Raton, FL: Taylor & Francis. doi: 10.1201/9781351069892

Seybold, A. C., Wharton, D. A., Thorne, M. A. S., & Marshall, C. J. (2017). Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1. Biology Open, 6, 1953-1959. doi: 10.1242/bio.023341

Chapter in Book - Other

Morgan-Richards, M., Marshall, C. J., Biggs, P. J., & Trewick, S. A. (2023). Insect freeze-tolerance Downunder: The microbial connection. Insects, 14(1), 89. doi: 10.3390/insects14010089

Journal - Research Article

Thorne, M. A. S., Seybold, A., Marshall, C., & Wharton, D. (2017). Molecular snapshot of an intracellular freezing event in an Antarctic nematode. Cryobiology, 75, 117-124. doi: 10.1016/j.cryobiol.2017.01.003

Journal - Research Article

Wharton, D. A., Marshall, C. J., & Egeter, B. (2017). Comparisons between two Antarctic nematodes: Cultured Panagrolaimus sp. DAW1 and field-sourced Panagrolaimus davidi. Nematology, 19(5), 533-542. doi: 10.1163/15685411-00003066

Journal - Research Article

Marshall, C. J., Basu, K., & Davies, P. L. (2016). Ice-shell purification of ice-binding proteins. Cryobiology, 72(3), 258-263. doi: 10.1016/j.cryobiol.2016.03.009

Journal - Research Article

Papetti, C., Windisch, H. S., La Mesa, M., Lucassen, M., Marshall, C., & Lamare, M. D. (2016). Non-Antarctic notothenioids: Past phylogenetic history and contemporary phylogeographic implications in the face of environmental changes. Marine Genomics, 25. doi: 10.1016/j.margen.2015.11.007

Journal - Research Article

Kasparova, E., Van de Putte, A. P., Marshall, C., & Janko, K. (2015). Lifestyle and ice: The relationship between ecological specialization and response to Pleistocene climate change. PLoS ONE, 10(11), e0138766. doi: 10.1371/journal.pone.0138766

Journal - Research Article

Hawes, T. C., Marshall, C. J., & Wharton, D. A. (2014). A 9kDa antifreeze protein from the Antarctic springtail, Gomphiocephalus hodgsoni. Cryobiology, 69(1), 181-183. doi: 10.1016/j.cryobiol.2014.07.001

Journal - Research Article

MacKenzie, A. L., & Marshall, C. (2014). Polysaccharidases in the crystalline styles of selectively bred greenshell mussel (Perna canaliculus Gmelin) families. Journal of Shellfish Research, 33(3), 687-694. doi: 10.2983/035.033.0303

Journal - Research Article

MacKenzie, A. L., & Marshall, C. (2014). Proteins in the crystalline styles of the marine mussels Perna Canaliculus Gmelin and Mytilus galloprovincialis Lamarck. Journal of Shellfish Research, 33(3), 673-685. doi: 10.2983/035.033.0302

Journal - Research Article

Raymond, M. R., Wharton, D. A., & Marshall, C. J. (2014). Nematodes from the Victoria Land coast, Antarctica and comparisons with cultured Panagrolaimus davidi. Antarctic Science, 26(1), 15-22. doi: 10.1017/S0954102013000230

Journal - Research Article

Thorne, M. A. S., Kagoshima, H., Clark, M. S., Marshall, C. J., & Wharton, D. A. (2014). Molecular analysis of the cold tolerant Antarctic nematode, Panagrolaimus davidi. PLoS ONE, 9(8), e104526. doi: 10.1371/journal.pone.0104526

Journal - Research Article

Raymond, M. R., Wharton, D. A., & Marshall, C. J. (2013). Factors determining nematode distributions at Cape Hallett and Gondwana station, Antarctica. Antarctic Science, 25(3), 347-357. doi: 10.1017/S0954102012001162

Journal - Research Article

Hawes, T. C., Marshall, C. J., & Wharton, D. A. (2012). Ultraviolet radiation tolerance of the Antarctic springtail, Gomphiocephalus hodgsoni. Antarctic Science, 24(2), 147-153. doi: 10.1017/S0954102011000812

Journal - Research Article

MacKenzie, L. A., Selwood, A. I., & Marshall, C. (2012). Isolation and characterization of an enzyme from the Greenshell™ mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid. Toxicon, 60(3), 406-419. doi: 10.1016/j.toxicon.2012.05.005

Journal - Research Article

Van de Putte, A. P., Janko, K., Kasparova, E., Maes, G. E., Rock, J., Koubbi, P., … Marshall, C. (2012). Comparative phylogeography of three trematomid fishes reveals contrasting genetic structure patterns in benthic and pelagic species. Marine Genomics, 8, 23-34. doi: 10.1016/j.margen.2012.05.002

Journal - Research Article

Hawes, T. C., Marshall, C. J., & Wharton, D. A. (2011). Antifreeze proteins in the Antarctic springtail, Gressittacantha terranova. Journal of Comparative Physiology B, 181(6), 713-719. doi: 10.1007/s00360-011-0564-4

Journal - Research Article

Janko, K., Marshall, C., Musilová, Z., Van Houdt, J., Couloux, A., Cruaud, C., & Lecointre, G. (2011). Multilocus analyses of an Antarctic fish species flock (Teleostei, Notothenioidei, Trematominae): Phylogenetic approach and test of the early-radiation event. Molecular Phylogenetics & Evolution, 60(3), 305-316. doi: 10.1016/j.ympev.2011.03.008

Journal - Research Article

Wharton, D. A., Selvanesan, L., & Marshall, C. J. (2010). Ice-active proteins from New Zealand snow tussocks, Chionochloa macra and c. Rigida. CryoLetters, 31(3), 239-248.

Journal - Research Article

Isely, N., Lamare, M., Marshall, C., & Barker, M. (2009). Expression of the DNA repair enzyme, photolyase, in developmental tissues and larvae, and in response to ambient UV-R in the Antarctic sea urchin Sterechinus neumayeri. Photochemistry & Photobiology, 85(5), 1168-1176. doi: 10.1111/j.1751-1097.2009.00566.x

Journal - Research Article

Wharton, D. A., & Marshall, C. J. (2009). How do terrestrial Antarctic organisms survive in their harsh environment? Journal of Biology, 8, 39. doi: 10.1186/jbiol142

Journal - Research Article

Wharton, D. A., Pow, B., Kristensen, M., Ramløv, H., & Marshall, C. J. (2009). Ice-active proteins and cryoprotectants from the New Zealand alpine cockroach, Celatoblatta quinquemaculata. Journal of Insect Physiology, 55(1), 27-31. doi: 10.1016/j.jinsphys.2008.09.007

Journal - Research Article

Kumble, K. D., Demmer, J., Fish, S., Hall, C., Corrales, S., DeAth, A., … Luxmanan, S., Marshall, C. J., & Wharton, D. A. (2008). Characterization of a family of ice-active proteins from the Ryegrass, Lolium perenne. Cryobiology, 57(3), 263-268. doi: 10.1016/j.cryobiol.2008.09.005

Journal - Research Article

Smith, T., Wharton, D. A., & Marshall, C. J. (2008). Cold tolerance of an Antarctic nematode that survives intracellular freezing: Comparisons with other nematode species. Journal of Comparative Physiology B, 178(1), 93-100. doi: 10.1007/s00360-007-0202-3

Journal - Research Article

Janko, K., Lecointre, G., DeVries, A., Couloux, A., Cruaud, C., & Marshall, C. (2007). Did glacial advances during the Pleistocene influence differently the demographic histories of benthic and pelagic Antarctic shelf fishes? Inferences from intraspecific mitochondrial and nuclear DNA sequence diversity. BMC Evolutionary Biology, 7, 220. doi: 10.1186/1471-2148-7-220

Journal - Research Article

Wharton, D. A., Wilson, P. W., Mutch, J. S., Marshall, C. J., & Lim, M. (2007). Recrystallization inhibition assessed by splat cooling and optical recrystallometry. CryoLetters, 28(1), 61-68.

Journal - Research Article

Lamare, M. D., Barker, M. F., Lesser, M. P., & Marshall, C. (2006). DNA photorepair in echinoid embryos: Effects of temperature on repair rate in Antarctic and non-Antarctic species. Journal of Experimental Biology, 209, 5017-5028. doi: 10.1242/jeb.02598

Journal - Research Article

Wharton, D. A., Barrett, J., Goodall, G., Marshall, C. J., & Ramløv, H. (2005). Ice-active proteins from the Antarctic nematode Panagrolaimus davidi. Cryobiology, 51, 198-207.

Journal - Research Article

Wharton, D. A., Downes, M. F., Goodall, G., & Marshall, C. J. (2005). Freezing and cryoprotective dehydration in an Antarctic nematode (Panagrolaimus davidi) visualised using a freeze substitution technique. Cryobiology, 50, 21-28.

Journal - Research Article

Wharton, D. A., Mutch, J. S., Wilson, P. W., Marshall, C. J., & Lim, M. (2004). A simple ice nucleation spectrometer. CryoLetters, 25, 335-340.

Journal - Research Article

Wharton, D. A., Goodall, G., & Marshall, C. J. (2003). Freezing survival and cryoprotective dehydration as cold tolerance mechanisms in the Antarctic nematode Panagrolaimus davidi. Journal of Experimental Biology, 206, 215-221.

Journal - Research Article

Denton, M. J., Marshall, C. J., & Legge, M. (2002). The protein folds as platonic forms: New support for the pre-darwinian conception of evolution by natural law. Journal of Theoretical Biology, 219(3), 325-342.

Journal - Research Article

Wharton, D. A., Goodall, G., & Marshall, C. J. (2002). Freezing rate affects the survival of a short-term freezing stress in Panagrolaimus davidi, an Antarctic nematode that survives intracellular freezing. CryoLetters, 23, 5-10.

Journal - Research Article

Denton, M. J., & Marshall, C. J. (2001). Laws of form revisited. Nature, 410, 417.

Journal - Research Article

Denton, M. J., & Marshall, C. J. (2001). Laws of form revisted. Concepts, 410, 417.

Journal - Research Article

Sharpe, M., Love, C., & Marshall, C. (2001). Lactate dehydrogenase from the Antarctic eelpout, Lycodichthys dearborni. Polar Biology, 24(4), 258-269. doi: 10.1007/s003000000206

Journal - Research Article

Piotte, C. P., Hunter, A. K., Marshall, C. J., & Grigor, M. R. (1998). Phylogenetic analysis of three lipocalin-like proteins present in the milk of Trichosurus vulpecula (Phalangeridae, Marsupialia). Journal of Molecular Evolution, 46, 361-369.

Journal - Research Article

Marshall, C. J. (1997). Cold-adapted enzymes. Trends in Biotechnology, 15(9), 359-364.

Journal - Research Article

Piotte, C. P., Marshall, C. J., Hubbard, M. J., Collet, C., & Grigor, M. R. (1997). Lysozyme and α-lactalbumin from the milk of a marsupial, the common brush-tailed possum (Trichosurus vulpecula). Biochimica et Biophysica Acta: General Subjects, 1336(2), 235-242. doi: 10.1016/S0304-4165(97)00033-0

Journal - Research Article

Cutfield, S. M., Dodson, E. J., Anderson, B. F., Marshall, C. J., Moody, P. C., Sullivan, P. A., & Cutfield, J. F. (1995). The crystal structure of a major secreted aspartic proteinase from Candida albicans in complexes with two inhibitors. Structure, 3, 1261-1271.

Journal - Research Article

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Associate Professor Craig Marshall

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