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Juliet PullarResearch Fellow

BSc(Hons), PhD, DPH (Otago)

Email juliet.pullar@otago.ac.nz
Tel +64 3 364 1559

Research interests

Dr Juliet Pullar obtained her PhD at the University of Otago, Christchurch under the supervision of Professors’ Margreet Vissers and Christine Winterbourn. Her work was focused on thiol biochemistry, uncovering targets of the neutrophil-derived oxidant hypochlorous acid in endothelial cells.

Dr Pullar continued onto a NZ Science and Technology Postdoctoral Fellowship in the Centre for Free Radical Research with Professor Mark Hampton studying the redox regulation of apoptosis. She has expertise in cell biology, cell culture, flow cytometry, ELISA, HPLC, spectrophotometry, microscopy, neutrophil function, fertility analysis, electrophoresis and western blotting.

More recently Dr Pullar has been working with Professor Margreet Vissers investigating the biological functions of vitamin C. This has allowed her to gain further expertise in carrying out human studies. Her current projects include investigating the use of red blood cell ascorbate as an indicator of vitamin C status, understanding the pharmacokinetics of vitamin C in the skin, and the role of vitamin C in the healing of chronic wounds.

She completed a Diploma in Public Health in 2010. Dr Pullar teaches part of the BBiomedSc(Hons) programme, has co-supervised three summer students and is currently co-supervising one PhD student and one Masters student.

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Publications

Pullar, J. M., Dunham, S., Dachs, G. U., Vissers, M. C. M., & Carr, A. C. (2020). Erythrocyte ascorbate is a potential indicator of steady-state plasma ascorbate concentrations in healthy non-fasting individuals. Nutrients, 12(2), 418. doi: 10.3390/nu12020418

Vissers, M. C. M., Pullar, J. M., & Peskin, A. V. (2018). Myeloperoxidase-derived oxidants hypochlorous acid and chloramines: Microbicidal marvels and inflammatory mischief makers. In M. C. M. Vissers, M. B. Hampton & A. J. Kettle (Eds.), Hydrogen peroxide metabolism in health and disease. (pp. 305-326). Boca Raton, FL: CRC Press.

Pullar, J. M., Carr, A. C., Bozonet, S. M., & Vissers, M. C. M. (2018). High vitamin C status is associated with elevated mood in male tertiary students. Antioxidants, 7(7), 91. doi: 10.3390/antiox7070091

Pullar, J. M., Bayer, S., & Carr, A. C. (2018). Appropriate handling, processing and analysis of blood samples is essential to avoid oxidation of vitamin C to dehydroascorbic acid. Antioxidants, 7(2), 29. doi: 10.3390/antiox7020029

Ang, A., Pullar, J. M., Currie, M. J., & Vissers, M. C. M. (2018). Vitamin C and immune cell function in inflammation and cancer. Biochemical Society Transactions, 46, 1147-1159. doi: 10.1042/bst20180169

Chapter in Book - Research

Vissers, M. C. M., Pullar, J. M., & Peskin, A. V. (2018). Myeloperoxidase-derived oxidants hypochlorous acid and chloramines: Microbicidal marvels and inflammatory mischief makers. In M. C. M. Vissers, M. B. Hampton & A. J. Kettle (Eds.), Hydrogen peroxide metabolism in health and disease. (pp. 305-326). Boca Raton, FL: CRC Press.

Vissers, M. C. M., Carr, A. C., Pullar, J. M., & Bozonet, S. M. (2013). The bioavailability of vitamin C from kiwifruit. In M. Boland & P. J. Moughan (Eds.), Nutritional benefits of kiwifruit. (pp. 125-147). Waltham, MA: Academic Press. doi: 10.1016/b978-0-12-394294-4.00007-9

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Journal - Research Article

Pullar, J. M., Dunham, S., Dachs, G. U., Vissers, M. C. M., & Carr, A. C. (2020). Erythrocyte ascorbate is a potential indicator of steady-state plasma ascorbate concentrations in healthy non-fasting individuals. Nutrients, 12(2), 418. doi: 10.3390/nu12020418

Pullar, J. M., Carr, A. C., Bozonet, S. M., & Vissers, M. C. M. (2018). High vitamin C status is associated with elevated mood in male tertiary students. Antioxidants, 7(7), 91. doi: 10.3390/antiox7070091

Pullar, J. M., Bayer, S., & Carr, A. C. (2018). Appropriate handling, processing and analysis of blood samples is essential to avoid oxidation of vitamin C to dehydroascorbic acid. Antioxidants, 7(2), 29. doi: 10.3390/antiox7020029

Pullar, J. M., Carr, A. C., & Vissers, M. C. M. (2017). The roles of vitamin C in skin health. Nutrients, 9(8), 866. doi: 10.3390/nu9080866

Pearson, J. F., Pullar, J. M., Wilson, R., Spittlehouse, J. K., Vissers, M. C. M., Skidmore, P. M. L., Willis, J., Cameron, V. A., & Carr, A. C. (2017). Vitamin C status correlates with markers of metabolic and cognitive health in 50-year-olds: Findings of the CHALICE Cohort Study. Nutrients, 9(8), 831. doi: 10.3390/nu9080831

Pullar, J. M., Carr, A. C., Bozonet, S. M., Rosengrave, P., Kettle, A. J., & Vissers, M. C. M. (2017). Elevated seminal plasma myeloperoxidase is associated with a decreased sperm concentration in young men. Andrology, 5(3), 431-438. doi: 10.1111/andr.12327

Carr, A. C., Pullar, J. M., Bozonet, S. M., & Vissers, M. C. M. (2016). Marginal ascorbate status (Hypovitaminosis C) results in an attenuated response to vitamin C supplementation. Nutrients, 8(6), 341. doi: 10.3390/nu8060341

Bozonet, S. M., Carr, A. C., Pullar, J. M., & Vissers, M. C. M. (2015). Enhanced human neutrophil vitamin C status, chemotaxis and oxidant generation following dietary supplementation with vitamin C-rich SunGold kiwifruit. Nutrients, 7(4), 2574-2588. doi: 10.3390/nu7042574

Carr, A. C., Bozonet, S. M., Pullar, J. M., & Vissers, M. C. M. (2013). Mood improvement in young adult males following supplementation with gold kiwifruit, a high-vitamin C food. Journal of Nutritional Science, 2, e24. doi: 10.1017/jns.2013.12

Carr, A. C., Bozonet, S. M., Pullar, J. M., Simcock, J. W., & Vissers, M. C. M. (2013). A randomized steady-state bioavailability study of synthetic versus natural (kiwifruit-derived) vitamin C. Nutrients, 5(9), 3684-3695. doi: 10.3390/nu5093684

Carr, A. C., Bozonet, S. M., Pullar, J. M., Simcock, J. W., & Vissers, M. C. M. (2013). Human skeletal muscle ascorbate is highly responsive to changes in vitamin C intake and plasma concentrations. American Journal of Clinical Nutrition, 97, 800-807. doi: 10.3945/ajcn.112.053207

Carr, A. C., Pullar, J. M., Moran, S., & Vissers, M. C. M. (2012). Bioavailability of vitamin C from kiwifruit in non-smoking males: Determination of ‘healthy’ and ‘optimal’ intakes. Journal of Nutritional Science, 1, e14. doi: 10.1017/jns.2012.15

Cox, A. G., Pearson, A. G., Pullar, J. M., Jönsson, T. J., Lowther, W. T., Winterbourn, C. C., & Hampton, M. B. (2009). Mitochondrial peroxiredoxin 3 is more resilient to hyperoxidation than cytoplasmic peroxiredoxins. Biochemical Journal, 421(1), 51-58. doi: 10.1042/BJ20090242

Cox, A. G., Pullar, J. M., Hughes, G., Ledgerwood, E. C., & Hampton, M. B. (2008). Oxidation of mitochondrial peroxiredoxin 3 during the initiation of receptor-mediated apoptosis. Free Radical Biology & Medicine, 44(6), 1001-1009. doi: 10.1016/j.freeradbiomed.2007.11.017

Thomson, S. J., Cox, A. G., Cuddihy, S. L., Pullar, J. M., & Hampton, M. B. (2008). Inhibition of receptor-mediated apoptosis upon Bcl-2 overexpression is not associated with increased antioxidant status. Biochemical & Biophysical Research Communications, 375(1), 145-150. doi: 10.1016/j.bbrc.2008.07.133

Thomson, S. J., Brown, K. K., Pullar, J. M., & Hampton, M. B. (2006). Phenethyl isothiocyanate triggers apoptosis in Jurkat cells made resistant by the overexpression of Bcl-2. Cancer Research, 66(13), 6772-6777.

Pullar, J. M., Thomson, S. J., King, M. J., Turnbull, C. I., Midwinter, R. G., & Hampton, M. B. (2004). The chemopreventive agent phenethyl isothiocyanate sensitizes cells to Fas-mediated apoptosis. Carcinogenesis, 25(5), 765-772.

Pullar, J. M., & Hampton, M. B. (2002). Diphenyleneiodonium triggers the efflux of glutathione from cultured cells. Journal of Biological Chemistry, 277(22), 19402-19407.

Pullar, J., Winterbourn, C. C., & Vissers, M. C. M. (2002). The effect of hypochlorous acid on the expression of adhesion molecules and activation of NF-κ in cultured human endothelial cells. Antioxidants & Redox Signaling, 4(1), 5-15.

Pullar, J. M., Vissers, M. C. M., & Winterbourn, C. C. (2001). Glutathione oxidation by hypochlorous acid in endothelial cells produces glutathione sulfonamide as a major product but not glutathione disulfide. Journal of Biological Chemistry, 276(25), 22120-22125. doi: 10.1074/jbc.M102088200

Pullar, J. M., Vissers, M. C. M., & Winterbourn, C. C. (2000). Living with a killer: The effects of hypochlorous acid on mammalian cells. IUBMB Life, 50, 259-266.

Pullar, J. M., Winterbourn, C. C., & Vissers, M. C. M. (1999). Loss of GSH and thiol enzymes in endothelial cellls exposed to sublethal concentrations of hypochlorous acid. American Journal of Physiology: Heart & Circulatory Physiology, 277, H1505-H1512.

Vissers, M. C. M., Pullar, J. M., & Hampton, M. B. (1999). Hypochlorous acid causes caspase activation and apoptosis or growth arrest in human endothelial cells. Biochemical Journal, 344, 443-449.

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Journal - Research Other

Ang, A., Pullar, J. M., Currie, M. J., & Vissers, M. C. M. (2018). Vitamin C and immune cell function in inflammation and cancer. Biochemical Society Transactions, 46, 1147-1159. doi: 10.1042/bst20180169

Carr, A., Pullar, J., Forbes, L., & Rosengrave, P. (2014). Working mothers are disadvantaged by limited funding for health research. New Zealand Medical Journal, 127(1398). Retrieved from http://www.nzma.org.nz/journal

Pullar, J. M., Carr, A. C., & Vissers, M. C. M. (2013). Vitamin C supplementation and kidney stone risk. New Zealand Medical Journal, 126(1384). Retrieved from http://www.nzma.org.nz/journal

Carr, A. C., Pullar, J. M., & Vissers, M. C. M. (2013). Beating the blues: The association between fruit and vegetable intake and improved mood. New Zealand Medical Journal, 126(1384). Retrieved from http://www.nzma.org.nz/journal

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Conference Contribution - Published proceedings: Abstract

Carr, A., Pullar, J., Bozonet, S., & Vissers, M. (2015). Marginal ascorbate status (hypovitaminosis C) results in an attenuated response to supplementation. Proceedings of the Nutrition Society of Australia, 39, (pp. 71). Sydney, Australia: Nutrition Society of Australia. [Abstract]

Carr, A. C., Bozonet, S. M., Pullar, J. M., & Vissers, M. C. M. (2013). Mood and vitality improvement in young adult males following supplementation with gold kiwifruit, a high vitamin C food. Australasian Medical Journal, 6(11), (pp. 667). [Abstract]

Carr, A. C., Pullar, J. M., Bozonet, S. M., Moran, S., & Vissers, M. C. M. (2013). Bioavailability of vitamin C from a common food source (kiwifruit) in young adult males. Australasian Medical Journal, 6(11), (pp. 658). [Abstract]

Cox, A. G., Pullar, J. M., Hughes, G., Ledgerwood, E. C., & Hampton, M. B. (2007). Mitochondrial peroxiredoxin 3 is oxidized during Fas and TNF-a mediated apoptosis. Proceedings of the American Association for Cancer Research (AACR) 98th Annual Meeting. Retrieved from http://www.aacrmeetingabstracts.org/

Pullar, J. M., & Hampton, M. B. (2002). Diphenyleneiodonium triggers the efflux of glutathione from cultured cells. Free Radical Biology and Medicine. 33(Suppl. 1), (pp. S91-S92). [Abstract]

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Conference Contribution - Poster Presentation (not in published proceedings)

Carr, A. C., Bozonet, S. M., Pullar, J. M., Simcock, J. W., & Vissers, M. C. M. (2013, August). Human skeletal muscle ascorbate is highly responsive to changes in vitamin C intake and plasma concentrations. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Carr, A. C., Bozonet, S. M., Pullar, J. M., & Vissers, M. C. M. (2013, August). Mood improvement and reduced fatigue in young adult males following supplementation with kiwifruit, a high vitamin C food. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Thomson, S. J., Pullar, J. M., & Hampton, M. B. (2004, December). Phenethyl isothiocyanate triggers apoptosis in cells over-expressing Bcl-2. Poster session presented at the 13th Annual Conference of the Society for Free Radical Research (Australasia), Christchurch, New Zealand.

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Conference Contribution - Verbal presentation and other Conference outputs

Vissers, M. C. M., Kuiper, C., Dachs, G. U., Campbell, E., Pujary, U.-R., Bozonet, S. M., Pullar, J. M., & Carr, A. C. (2013, August). The role of vitamin C in managing metabolic stress and its impact in tumour biology and inflammation. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Pullar, J. M., Baty, J. W., Csordas, A., Winterbourn, C. C., & Hampton, M. B. (2005, December). Oxidation of thiol proteins in cultured cells: Comparison of H2O2 with receptor-mediated signalling. Verbal presentation at the 3rd Joint Meeting of the Society for Free Radical Research Australasia and Japan, Queensland, Australia.

Thomson, S. J., Pullar, J., Baty, J. W., & Winterbourn, C. C. (2002, November). Monitoring oxidation of the thiol proteins during apoptosis. Verbal presentation at the Microbes and Molecules Conference, Christchurch, New Zealand.

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Awarded Doctoral Degree

Pullar, J. M. (2000). The reaction of hypochlorous acid with human endothelial cells; consequencees and identification of targets (PhD). University of Otago, Dunedin, New Zealand.

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