Contact Details
- Phone
- 64 3 479 5290
- erwin.lamping@otago.ac.nz
University Links
- Position
- Senior Research Fellow
- Department
- Department of Oral Sciences
- Qualifications
- PhD
- Research summary
- Membrane protein biology
- Teaching
Dr Lamping supervises undergraduate and postgraduate students, and enjoys sharing his skills and experience with like-minded individuals.
- Memberships
- Member of the American Society for Microbiology, the New Zealand Microbiological Society, the New Zealand Society for Biochemistry and Molecular Biology, as well as member of the Oral Microbiology and Dental Health Research Theme and the Webster Centre for Infectious Diseases of the University of Otago.
Research
Experienced in molecular biology with expertise in biochemistry, yeast genetics, and molecular biology, Dr Lamping is particularly interested in studying the structure and function of eukaryotic membrane proteins—especially integral membrane proteins associated with multidrug resistance of fungal pathogens and human cancer cells. The list of membrane proteins includes:
- The azole antifungal drug target ERG11, an essential enzyme of ergosterol biosynthesis
- Multidrug efflux pumps of the pleiotropic drug resistance (PDR) and the multidrug resistance (MDR) family of efflux transporters
A significant part of his studies are dedicated to the continuous improvement of the basic molecular biology toolkit that enables scientists to ever more efficiently, precisely, and predictably express heterologous membrane proteins in the eukaryotic model organism Saccharomyces cerevisiae.
In the past decade he helped create and optimise a patented yeast membrane protein expression system used worldwide by over 50 research teams. Dr Lamping used that patented technology to create a suit of yeast strains overexpressing the azole drug target ERG11, as well as many PDR and MDR efflux transporters from human and important fungal pathogens: Candida albicans, C. glabrata, C. krusei, Cryptococcus neoformans, and Aspergillus fumigatus.
These strains have been used successfully for many different research applications funded by national (Marsden, HRC, and FRST), and international funding agencies (NIH, USA; Japan Health Sciences Foundation; and Japan Society for the Promotion of Sciences), and they helped solve the first crystal structure for any full-length cytochrome P450 enzyme, S. cerevisiae Erg11p.
Publications
Krishnan, C., Li, K. C., Lyons, K., Lamping, E., Tompkins, G., & Cannon, R. (2022). Evaluating the suitability of Electrolysed Oxidising Water as a denture disinfectant. New Zealand Dental Journal, 118(4), (pp. 169). [Abstract] Conference Contribution - Published proceedings: Abstract
Alhumaidi, M., Nentwig, L.-M., Rahman, H., Schmitt, L., Rudrow, A., Harris, A., … Lamping, E., … Golin, J. (2022). Residues forming the gating regions of asymmetric multidrug transporter Pdr5 also play roles in conformational switching and protein folding. Journal of Biological Chemistry, 298(12), 102689. doi: 10.1016/j.jbc.2022.102689 Journal - Research Article
James, J. E., Santhanam, J., Cannon, R. D., & Lamping, E. (2022). Voriconazole treatment induces a conserved sterol/pleiotropic drug resistance regulatory network, including an alternative ergosterol biosynthesis pathway, in the clinically important FSSC species, Fusarium keratoplasticum. Journal of Fungi, 8(10), 1070. doi: 10.3390/jof8101070 Journal - Research Article
James, J. E., Santhanam, J., Zakaria, L., Mamat Rusli, N., Abu Bakar, M., Suetrong, S., … Lamping, E., & Cannon, R. D. (2022). Morphology, phenotype, and molecular identification of clinical and environmental Fusarium solani species complex isolates from Malaysia. Journal of Fungi, 8(8), 845. doi: 10.3390/jof8080845 Journal - Research Article
Niimi, M., Niimi, K., Tanabe, K., Cannon, R. D., & Lamping, E. (2022). Inhibitor resistant mutants give important insights into Candida albicans ABC transporter Cdr1 substrate specificity and help elucidate efflux pump inhibition. Antimicrobial Agents & Chemotherapy, 66(1), e01748-21. doi: 10.1128/aac.01748-21 Journal - Research Article
2022
Journal - Research Article
Alhumaidi, M., Nentwig, L.-M., Rahman, H., Schmitt, L., Rudrow, A., Harris, A., … Lamping, E., … Golin, J. (2022). Residues forming the gating regions of asymmetric multidrug transporter Pdr5 also play roles in conformational switching and protein folding. Journal of Biological Chemistry, 298(12), 102689. doi: 10.1016/j.jbc.2022.102689
James, J. E., Santhanam, J., Cannon, R. D., & Lamping, E. (2022). Voriconazole treatment induces a conserved sterol/pleiotropic drug resistance regulatory network, including an alternative ergosterol biosynthesis pathway, in the clinically important FSSC species, Fusarium keratoplasticum. Journal of Fungi, 8(10), 1070. doi: 10.3390/jof8101070
James, J. E., Santhanam, J., Zakaria, L., Mamat Rusli, N., Abu Bakar, M., Suetrong, S., … Lamping, E., & Cannon, R. D. (2022). Morphology, phenotype, and molecular identification of clinical and environmental Fusarium solani species complex isolates from Malaysia. Journal of Fungi, 8(8), 845. doi: 10.3390/jof8080845
Niimi, M., Niimi, K., Tanabe, K., Cannon, R. D., & Lamping, E. (2022). Inhibitor resistant mutants give important insights into Candida albicans ABC transporter Cdr1 substrate specificity and help elucidate efflux pump inhibition. Antimicrobial Agents & Chemotherapy, 66(1), e01748-21. doi: 10.1128/aac.01748-21
Conference Contribution - Published proceedings: Abstract
Krishnan, C., Li, K. C., Lyons, K., Lamping, E., Tompkins, G., & Cannon, R. (2022). Evaluating the suitability of Electrolysed Oxidising Water as a denture disinfectant. New Zealand Dental Journal, 118(4), (pp. 169). [Abstract]
2021
Journal - Research Article
James, J. E., Lamping, E., Santhanam, J., & Cannon, R. D. (2021). PDR transporter ABC1 is involved in the innate azole resistance of the human fungal pathogen Fusarium keratoplasticum. Frontiers in Microbiology, 12, 673206. doi: 10.3389/fmicb.2021.673206
Madani, G., Lamping, E., Lee, H. J., Niimi, M., Mitra, A. K., & Cannon, R. D. (2021). Small-scale plasma membrane preparation for the analysis of Candida albicans Cdr1-mGFPHis. Journal of Visualized Experiments, 172, e62592. doi: 10.3791/62592
Madani, G., Lamping, E., & Cannon, R. D. (2021). Engineering a cysteine-deficient functional Candida albicans Cdr1 molecule reveals a conserved region at the cytosolic apex of ABCG transporters important for correct folding and trafficking of Cdr1. mSphere, 6(1), e01318-20. doi: 10.1128/mSphere.01318-20
Conference Contribution - Published proceedings: Abstract
Lamping, E., Niimi, M., Niimi, K., & Cannon, R. D. (2021). Substrate specificity of Candida albicans ATP-binding casette transporter Cdr1 responsible for multidrug resistance. Proceedings of the 16th Congress of the Federation of Asian & Oceanic Biochemists & Molecular Biologists (FAOBMB). (pp. 62). Retrieved from https://www.faobmb2021.org
Thiyahuddin, N., Rich, A. M., Lamping, E., & Cannon, R. D. (2021). Microevolution of Candida albicans strains in older people with low salivary flow. Oral Surgery, Oral Medicine, Oral Pathology & Oral Radiology, 132(1), (pp. e48). doi: 10.1016/j.oooo.2021.03.172
2020
Journal - Research Article
James, J. E., Lamping, E., Santhanam, J., Milne, T. J., Razak, M. F. A., Zakaria, L., & Cannon, R. D. (2020). A 23 bp cyp51A promoter deletion associated with voriconazole resistance in clinical and environmental isolates of Neocosmospora keratoplastica. Frontiers in Microbiology, 11, 272. doi: 10.3389/fmicb.2020.00272
2019
Journal - Research Article
Mohd Thiyahuddin, N., Lamping, E., Rich, A. M., & Cannon, R. D. (2019). Yeast species in the oral cavities of older people: A comparison between people living in their own homes and those in rest homes. Journal of Fungi, 5(2), 30. doi: 10.3390/jof5020030
Tanabe, K., Bonus, M., Tomiyama, S., Miyoshi, K., Nagi, M., Niimi, K., … Cannon, R. D., Niimi, M., & Lamping, E. (2019). FK506 resistance of Saccharomyces cerevisiae Pdr5 and Candida albicans Cdr1 involves mutations in the transmembrane domains and extracellular loops. Antimicrobial Agents & Chemotherapy, 63(1), e01146-18. doi: 10.1128/aac.01146-18
Conference Contribution - Published proceedings: Abstract
Kumar, M., Lee, H. J., Cannon, R., & Lamping, E. (2019). Confirmation of three predicted disulphide bonds in Candida albicans Cdr1. Journal of Dental Research, 98(A), 0800. Retrieved from https://iadr.abstractarchives.com
Mohd Thiyahuddin, N., Lamping, E., Rich, A., & Cannon, R. D. (2019). Yeast species in the oral cavities of older people. New Zealand Dental Journal, 115, (pp. 165-166). [Abstract]
Thiyahuddin, N., Cannon, R., Lamping, E., & Rich, A. (2019). Candida species and strains in the oral cavities of the elderly: A comparison between people in home-based care and in aged-care facilities. Oral Surgery, Oral Medicine, Oral Pathology & Oral Radiology, 128(1), (pp. e69). doi: 10.1016/j.oooo.2019.02.167
Conference Contribution - Verbal presentation and other Conference outputs
Madani, G., Lamping, E., Bostina, M., Raunser, S., Hall, N., Mitra, A., & Cannon, R. D. (2019, September). Expression, purification, and negative staining of Candida albicans plasma membrane protein Cdr1. Verbal presentation at the Sir John Walsh Research Institute Research Day, Dunedin, New Zealand.
2018
Conference Contribution - Published proceedings: Abstract
Lamping, E. (2018). Structural and functional insights into fungal pleiotropic drug resistance transporters. Proceedings of the Joint Australasian Mycological Society (AMS) & Australian Society for Microbiology (ASM) Scientific Meeting. (pp. 16). Retrieved from https://www.australasianmycologicalsociety.com
Madani, G., Lamping, E., Ha, N. C., Mitra, A. K., & Cannon, R. D. (2018). Expression and purification of Candida albicans multidrug efflux pump Cdr1 for structural studies. New Zealand Dental Journal, 114(4), (pp. 189). [Abstract]
Niimi, M., Niimi, K., Saiwan, S., Assavachin, S., Chindamporn, A., Cannon, R. D., & Lamping, E. (2018). Mapping the interaction of the Candida albicans multidrug resistance ABC protein Cdr1 with pump inhibitor clorgyline that stimulates ATPase activity. Medical Mycology, 56(Suppl. 2), (pp. S60). doi: 10.1093/mmy/myy036
Niimi, K., Lamping, E., Watanasrisin, W., Yurayart, C., Saiwan, S., Chindamporn, A., Cannon, R. D., & Niimi, M. (2018). K577 in intracellular loop 1 of C. albicans ABC transporter Cdr1 plays a critical role in drug transport. Medical Mycology, 56(Suppl. 2), (pp. S132). doi: 10.1093/mmy/myy036
Conference Contribution - Poster Presentation (not in published proceedings)
Madani, G., Lamping, E., & Cannon, R. (2018, September). Structural characterization of the archetypal fungal efflux pump Candida Albicans Cdr1. Poster session presented at the Otago Spotlight Symposium on Infectious Disease, Wellington, New Zealand.
Conference Contribution - Verbal presentation and other Conference outputs
Madani, G., Lamping, E., Hall, N., Mitra, A., & Cannon, R. D. (2018, March). Expression and purification of Candida albicans multidrug efflux pump Cdr1 for structural studies. Verbal presentation at the ABC2018 Conference: The 7th Federation of European Biochemical Societies (FEBS) Special Meeting on ABC Proteins, Innsbruck, Austria.
2017
Chapter in Book - Research
Lamping, E., Madani, G., Lee, H. J., Niimi, M., & Cannon, R. D. (2017). Structure-function analyses of multidrug tansporters. In R. Prasad (Ed.), Candida albicans: Cellular and molecular biology. (2nd ed.) (pp. 379-406). Springer. doi: 10.1007/978-3-319-50409-4
Journal - Research Article
Lamping, E., Zhu, J.-y., Niimi, M., & Cannon, R. D. (2017). Role of ectopic gene conversion in the evolution of a Candida krusei pleiotropic drug resistance transporter family. Genetics, 205(4), 1619-1639. doi: 10.1534/genetics.116.194811
Reis de Sá, L. F., Toledo, F. T., Gonçalves, A. C., Sousa, B. A., dos Santos, A. A., Brasil, P. F., … Lamping, E., & Ferreira-Pereira, A. (2017). Synthetic organotellurium compounds sensitize drug-resistant Candida albicans clinical isolates to fluconazole. Antimicrobial Agents & Chemotherapy, 61(1), e01231-16. doi: 10.1128/aac.01231-16
Conference Contribution - Published proceedings: Abstract
Madani, G., Lamping, E., & Cannon, R. D. (2017). Purification of Cdr1 as a first step to overcome drug resistant Candida infections. New Zealand Dental Journal, 113(4), (pp. 56-57). [Abstract]
Lee, H. J., Lamping, E., & Cannon, R. D. (2017). Extracellular loop amino acid residues essential for Candida albicans Cdr1 multidrug efflux pump function. New Zealand Dental Journal, 113(4), (pp. 56). [Abstract]
Lamping, E., Lee, H. J., & Cannon, R. D. (2017). New structural and functional insights into the type II fungal ATP-binding cassette transporter Candida albicans Cdr1. Proceedings of the Annual Conference of the Genetics Society of Australasia (GSA) with the New Zealand Society for Biochemistry and Molecular Biology (NZSBMB). 746. Retrieved from http://gen2017.w.events4you.currinda.com
Conference Contribution - Verbal presentation and other Conference outputs
Madani, G., Lamping, E., Lee, H. J., & Cannon, R. (2017, September). Candida albicans Cdr1 cysteines are critical for pump function. Verbal presentation at the IADR New Zealand Section Colgate Poster Competition 2017, Adelaide, SA.
2016
Journal - Research Article
Watanasrisin, W., Iwatani, S., Oura, T., Tomita, Y., Ikushima, S., Chindamporn, A., Niimi, M., Niimi, K., Lamping, E., Cannon, R. D., & Kajiwara, S. (2016). Identification and characterization of Candida utilis multidrug efflux transporter CuCdr1p. FEMS Yeast Research, 16, fow042. doi: 10.1093/femsyr/fow042
Panapruksachat, S., Iwatani, S., Oura, T., Vanittanakom, N., Chindamporn, A., Niimi, K., Niimi, M., Lamping, E., Cannon, R. D., & Kajiwara, S. (2016). Identification and functional characterization of Penicillium marneffei pleiotropic drug resistance transporters ABC1 and ABC2. Medical Mycology, 54, 478-491. doi: 10.1093/mmy/myv117
Conference Contribution - Published proceedings: Abstract
Madani, G., Lamping, E., Lee, H. J., & Cannon, R. D. (2016). Role of cysteine residues in Candida albicans Cdr1p efflux pump expression and function. Proceedings of the Sir John Walsh Research Institute Research Symposium. (pp. 12). Retrieved from http://www.otago.ac.nz/sjwri/news/otago621026
van Wichen, A., Lamping, E., & Lee, H. J. (2016). Investigating Candida albicans Cdr1p dimerisation with NanoBRET™ technology. New Zealand Medical Journal, 129(1441), (pp. 108). Retrieved from http://www.nzma.org.nz/journal
Conference Contribution - Verbal presentation and other Conference outputs
Madani, G., Lamping, E., Lee, H. J., & Cannon, R. (2016, November). Contribution of cysteine residues to Candida albicans Cdr1p multidrug efflux pump expression and function. Verbal presentation at the Joint Meeting of the New Zealand Microbiological Society (NZMS) and the New Zealand Society for Biochemistry and Molecular Biology (NZSBMB), Christchurch, New Zealand.
2015
Conference Contribution - Published proceedings: Abstract
Cannon, R. D., Niimi, K., Harding, D. R. K., Holmes, A. R., Niimi, M., Monk, B. C., & Lamping, E. (2015). Understanding and overcoming antifungal drug resistance [Invited speaker]. Proceedings of the Australasian Mycological Society Scientific Meeting held jointly with the Australian Society for Microbiology Scientific Meeting. Retrieved from https://www.australasianmycologicalsociety.com
Lee, J., Lamping, E., Cannon, R., & Lee, H. (2015). Extracellular cysteines of Candida albicans Cdr1p affect its efflux-pump function. Journal of Dental Research, 94(Spec Iss B), 2343753. Retrieved from http://www.iadr.org/
Cannon, R., Lee, H., & Lamping, E. (2015). Overcoming antifungal drug resistance: A new target. Journal of Dental Research, 94(Spec Iss B), 2334952. Retrieved from http://www.iadr.org/
Cannon, R. D., Lee, H. J., & Lamping, E. (2015). Functional analysis of PDR ABC transporters from fungal pathogens of humans and plants. Proceedings of the New Zealand Microbiological Society (NZMS) 60th Conference. (pp. 27). Retrieved from http://www.nzmsconference.org.nz/
2014
Journal - Research Article
Keniya, M. V., Holmes, A. R., Niimi, M., Lamping, E., Gillet, J.-P., Gottesman, M. M., & Cannon, R. D. (2014). Drug resistance is conferred on the model yeast Saccharomyces cerevisiae by expression of full-length melanoma-associated human ATP-binding cassette transporter ABCB5. Molecular Pharmaceutics, 11(10), 3452-3462. doi: 10.1021/mp500230b
Conference Contribution - Verbal presentation and other Conference outputs
Lee, H. J., Lamping, E., & Cannon, R. D. (2014, November). Unravelling the importance of conserved extracellular loop motifs of the archetype fungal multidrug efflux pump Candida albicans Cdr1p. Verbal presentation at the Joint Meeting of the New Zealand Microbiological Society (NZMS) and the New Zealand Society for Biochemistry and Molecular Biology (NZSBMB), Wellington, New Zealand.
2013
Journal - Research Article
Lamping, E., Niimi, M., & Cannon, R. D. (2013). Small, synthetic, GC-rich mRNA stem-loop modules 5' proximal to the AUG start-codon predictably tune gene expression in yeast. Microbial Cell Factories, 12, 74. doi: 10.1186/1475-2859-12-74
Conference Contribution - Published proceedings: Abstract
Cannon, R., Niimi, K., Lamping, E., Huschmann, F. U., Niimi, M., Keniya, M. V., Tyndall, J. D. A., Holmes, A. R., & Monk, B. C. (2013). Overcoming antifungal drug resistance. Proceedings of the Sir John Walsh Research Institute Research Day. (pp. 6). Retrieved from http://sjwri.otago.ac.nz/research/sjwri/
2012
Journal - Research Article
Niimi, K., Harding, D. R. K., Holmes, A. R., Lamping, E., Niimi, M., Tyndall, J. D. A., Cannon, R. D., & Monk, B. C. (2012). Specific interactions between the Candida albicans ABC transporter Cdr1p ectodomain and a D-octapeptide derivative inhibitor. Molecular Microbiology, 85(4), 747-767. doi: 10.1111/j.1365-2958.2012.08140.x
Conference Contribution - Published proceedings: Abstract
Niimi, M., Tanabe, K., Niimi, K., Lamping, E., Nagi, M., Holmes, A. R., Keniya, M. V., Monk, B. C., & Cannon, R. D. (2012). Milbemycins are broad-spectrum fungal multidrug efflux pump inhibitors that interact directly with the transmembrane domains of Candida albicans Cdr1p and Cdr2p. Mycoses, 55(Suppl. 4), (pp. 130). doi: 10.1111/j.1439-0507.2012.02206.x
Niimi, K., Lamping, E., Niimi, M., & Cannon, R. D. (2012). Dissection of C-albicans ABC transporter Cdr1p: Large intracellular and extracellular loops are critical structural and functional elements. Mycoses, 55(Suppl. 4), (pp. 335). doi: 10.1111/j.1439-0507.2012.02206.x
2011
Journal - Research Article
Holmes, A. R., Keniya, M. V., Ivnitski-Steele, I., Monk, B. C., Lamping, E., Sklar, L. A., & Cannon, R. D. (2011). The monoamine oxidase A inhibitor clorgyline is a broad-spectrum inhibitor of fungal ABC and MFS transporter efflux pump activities which reverses the azole resistance of Candida albicans and Candida glabrata clinical isolates. Antimicrobial Agents & Chemotherapy, 56(3), 1508-1515. doi: 10.1128/aac.05706-11
Tanabe, K., Lamping, E., Nagi, M., Okawada, A., Holmes, A. R., Miyazaki, Y., Cannon, R. D., Monk, B. C., & Niimi, M. (2011). Chimeras of Candida albicans Cdr1p and Cdr2p reveal features of pleiotropic drug resistance transporter structure and function. Molecular Microbiology, 82(2), 416-433. doi: 10.1111/j.1365-2958.2011.07820.x
Conference Contribution - Published proceedings: Abstract
Cannon, R. D., Niimi, K., Lamping, E., Niimi, M., Holmes, A. R., & Monk, B. C. (2011). Understanding the inhibition of fungal drug efflux pumps. Journal of Dental Research, 90(Spec Iss B), 154838. Retrieved from http://www.dentalresearch.org
2010
Chapter in Book - Research
Lamping, E., & Cannon, R. D. (2010). Use of a yeast-based membrane protein expression technology to overexpress drug resistance efflux pumps. In G. J. Seymour, M. P. Cullinan & N. C. K. Heng (Eds.), Oral Biology. (pp. 219-250). New York: Springer. doi: 10.1007/978-1-60761-820-1_15
Journal - Research Article
Niimi, K., Monk, B. C., Hirai, A., Hatakenaka, K., Umeyama, T., Lamping, E., … Cannon, R. D., & Niimi, M. (2010). Clinically significant micafungin resistance in Candida albicans involves modification of a glucan synthase catalytic subunit GSC1 (FKS1) allele followed by loss of heterozygosity. Journal of Antimicrobial Chemotherapy, 65, 842-852. doi: 10.1093/jac/dkq073
Lamping, E., Baret, P. V., Holmes, A. R., Monk, B. C., Goffeau, A., & Cannon, R. D. (2010). Fungal PDR transporters: Phylogeny, topology, motifs and function. Fungal Genetics & Biology, 47(2), 127-142. doi: 10.1016/j.fgb.2009.10.007
Conference Contribution - Published proceedings: Abstract
Cannon, R., Niimi, K., Woods, M., Hirai, A., Umeyama, T., Hatakenaka, K., … Lamping, E., Niimi, M., & Monk, B. (2010). Echinocandin resistance in Candida species. Journal of Dental Research, 89(Spec Iss C). Retrieved from http://www.dentalresearch.org
Conference Contribution - Poster Presentation (not in published proceedings)
Holmes, A. R., Niimi, M., Lamping, E., Keniya, M. V., Niimi, K., Monk, B. C., & Cannon, R. D. (2010, February-March). Enhancing functional heterologous expression of human ABCB1 in Saccharomyces cerevisiae. Poster session presented at the 3rd FEBS Special Meeting: ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases, Innsbruck, Austria.
Conference Contribution - Verbal presentation and other Conference outputs
Cannon, R., Hayama, K., Lamping, E., Niimi, K., Keniya, M., Niimi, M., Holmes, A., … Monk, B. (2010, September). Drug discovery using fungal ABC transporters expressed in Saccharomyces cerevisiae. Verbal presentation at the 28th Small Meeting on Yeast Transport and Energetics (SMYTE), New Delhi, India.
2009
Journal - Research Article
Ivnitski-Steele, I., Holmes, A. R., Lamping, E., Monk, B. C., Cannon, R. D., & Sklar, L. A. (2009). Identification of Nile red as a fluorescent substrate of the Candida albicans ATP-binding cassette transporters Cdr1p and Cdr2p and the major facilitator superfamily transporter Mdr1p. Analytical Biochemistry, 394(1), 87-91. doi: 10.1016/j.ab.2009.07.001
Cannon, R. D., Lamping, E., Holmes, A. R., Niimi, K., Baret, P. V., Keniya, M. V., … Niimi, M., … Monk, B. C. (2009). Efflux-mediated antifungal drug resistance. Clinical Microbiology Reviews, 22(2), 291-321. doi: 10.1128/cmr.00051-08
Lamping, E., Ranchod, A., Nakamura, K., Tyndall, J. D. A., Niimi, K., Holmes, A. R., Niimi, M., & Cannon, R. D. (2009). Abc1p is a multidrug efflux transporter that tips the balance in favor of innate azole resistance in Candida krusei. Antimicrobial Agents & Chemotherapy, 53(2), 354-369. doi: 10.1128/AAC.01095-08
Conference Contribution - Published proceedings: Abstract
Cannon, R. D., Lamping, E., Niimi, K., Holmes, A. R., Monk, B. C., & Niimi, M. (2009). Functional expression of human ABCB1 in Saccharomyces cerevisiae. Proceedings of the ComBio2009 Conference. [CD-ROM] [Abstract]
Lamping, E., Baret, P. V., Holmes, A. R., Monk, B. C., Goffeau, A., & Cannon, R. D. (2009). Phylogenetic clustering and new motifs of fungal PDR ABC transporters. Proceedings of the ComBio2009 Conference. [CD-ROM] [Abstract]
Conference Contribution - Verbal presentation and other Conference outputs
Cannon, R. D., Lamping, E., Keniya, M. V., Niimi, K., Tanabe, K., Niimi, M., Monk, B. C., & Holmes, A. R. (2009, May). Azole resistance in Candida species. Verbal presentation at the 17th Congress of the International Society for Human and Animal Mycology (ISHAM): Medical Mycology in the 21st Century: Scientific Base and Anticipated Challenges, Tokyo, Japan.
2008
Journal - Research Article
Holmes, A. R., Lin, Y.-H., Niimi, K., Lamping, E., Keniya, M., Niimi, M., … Monk, B. C., & Cannon, R. D. (2008). ABC transporter Cdr1p contributes more than Cdr2p does to fluconazole efflux in fluconazole-resistant Candida albicans clinical isolates. Antimicrobial Agents & Chemotherapy, 52(11), 3851-3862. doi: 10.1128/AAC.00463-08
Conference Contribution - Published proceedings: Abstract
Raju, S., Holmes, A., Lamping, E., Monk, B., & Cannon, R. (2008). Functional analysis of phosphorylation sites in Candida albicans Cdr1p. Journal of Dental Research, 87(Spec Iss B), 3023. Retrieved from http://www.dentalresearch.org
Niimi, M., Tanabe, K., Niimi, K., Lamping, E., Takano, Y., Holmes, A. R., Monk, B. C., Cannon, R. D., & Uehara, Y. (2008). Characterization of drug efflux pump inhibitors that chemosensitize fungal ABC transporters. Proceedings of the 9th American Society for Microbiology Conference on Candida and Candidiasis. (pp. 50). Washington, DC: ASM. [Abstract]
Holmes, A. R., Raju, S., Uwamahoro, N., Niimi, K., Lamping, E., Keniya, M. V., Monk, B. C., & Cannon, R. D. (2008). Phosphorylation site S312 is important for Candida albicans Cdr1p function. Proceedings of the New Zealand Microbiological Society Microbiology Conference. (pp. 18-19). NZMS. Retrieved from http://www.nzmsconference08.org.nz/files/Oral-Abstracts-NZMS-2008.pdf
Kajiwara, S., Honda, T., Oura, T., Lamping, E., Keniya, M. V., Niimi, K., Niimi, M., Holmes, A. R., Monk, B. C., & Cannon, R. D. (2008). Alteration of Saccharomyces cerevisiae membrane composition for improved heterologous expression of human transporter ABCB1. Proceedings of the New Zealand Microbiological Society Microbiology Conference. (pp. 21). NZMS. Retrieved from http://www.nzmsconference08.org.nz/files/Poster-Abstracts-NZMS-2008.pdf
Conference Contribution - Poster Presentation (not in published proceedings)
Lamping, E., Nakamura, K., Tanabe, K., Niimi, K., Holmes, A. R., Takano, Y., … Cannon, R. D., & Niimi, M. (2008, September). Cryptococcus neoformans AFR2 and MDR1 are ABC-type multidrug efflux transporters able to pump azoles and many other xenobiotics. Poster session presented at the 7th International Conference on Cryptococcus & Cryptococcosis, Nagasaki, Japan.
Conference Contribution - Verbal presentation and other Conference outputs
Lamping, E., Niimi, K., Monk, B. C., Holmes, A. R., Niimi, M., & Cannon, R. D. (2008, March-April). Yeast: A promising host for the study of eukaryotic membrane proteins. Plenary presentation at the 2nd International Symposium on Advanced Biological Engineering and Science, Beijing, China.
2007
Journal - Research Article
Lamping, E., Monk, B. C., Niimi, K., Holmes, A. R., Tsao, S., Tanabe, K., Niimi, M., … Cannon, R. D. (2007). Characterization of three classes of membrane proteins involved in fungal azole resistance by functional hyperexpression in Saccharomyces cerevisiae. Eukaryotic Cell, 6(7), 1150-1165.
Tanabe, K., Lamping, E., Adachi, K., Takano, Y., Kawabata, K., Shizuri, Y., Niimi, M., & Uehara, Y. (2007). Inhibition of fungal ABC transporters by unnarmicin A and unnarmicin C, novel cyclic peptides from marine bacterium. Biochemical & Biophysical Research Communications, 364, 990-995.
Cannon, R. D., Lamping, E., Holmes, A. R., Niimi, K., Tanabe, K., & Monk, B. C. (2007). Candida albicans drug resistance: Another way to cope with stress. Microbiology, 153, 3211-3217.
Conference Contribution - Published proceedings: Abstract
Matejka, K. M., Tompkins, G. R., Lamping, E., & Monk, B. C. (2007). Characteristics of a variant phenotype of Moraxella catarrhalis. Proceedings of the Faculty of Dentistry 3M Research Day. Retrieved from http://dentistry.otago.ac.nz/research/rd2007/presentations.html
Lamping, E., Nakamura, K., Ranchod, A., Holmes, A. R., Niimi, K., Niimi, M., & Cannon, R. D. (2007). Mechanisms of innate azole resistance in Candida krusei. Proceedings of the Faculty of Dentistry 3M Research Day. Retrieved from http://dentistry.otago.ac.nz/research/rd2007/presentations.html
Cannon, R. D., Niimi, K., Holmes, A. R., Lamping, E., Keniya, M., Niimi, M., & Monk, B. C. (2007). Overcoming efflux pump-mediated drug resistance in Candida albicans. Journal of Dental Research, 86(Spec Iss A), 0247. Retrieved from http://www.dentalresearch.org
Niimi, K., Monk, B. C., Lamping, E., Holmes, A. R., Niimi, M., Maki, K., & Cannon, R. D. (2007). Echinocandin resistance mechanisms in Candida species. Proceedings of the Faculty of Dentistry 3M Research Day. Retrieved from http://dentistry.otago.ac.nz/research/rd2007/presentations.html
Holmes, A. R., Lin, Y.-H., Niimi, K., Lamping, E., Monk, B. C., Keniya, M., & Cannon, R. D. (2007). Differential expression and activities of drug efflux proteins Cdr1p and Cdr2p in azole resistant Candida albicans isolates. Proceedings of the Faculty of Dentistry 3M Research Day. Retrieved from http://dentistry.otago.ac.nz/research/rd2007/presentations.html
Raju, S., Holmes, A. R., Lamping, E., Monk, B. C., & Cannon, R. D. (2007). Functional analysis of phosphorylation sites in Candida albicans Cdr1p. Journal of Dental Research, 86(Spec Iss B). Retrieved from http://www.dentalresearch.org
Conference Contribution - Poster Presentation (not in published proceedings)
Holmes, A., Lin, Y.-H., Niimi, K., Lamping, E., Monk, B., Keniya, M., & Cannon, R. (2007, May). Differential expression and activities of drug efflux proteins Cdr1p and Cdr2p in azole resistant Candida albicans isolates. Poster session presented at the Second Federation of European Biochemical Societies Advanced Lecture Course, La Colle sur Loup, France.
Tanabe, K., Tomiyama, S., Lamping, E., Takano, Y., Uehara, Y., & Niimi, M. (2007, May). Novel mutations in a Saccharomyces cerevisiae ABC protein, Pdr5p, result in insensitivity to the efflux pump inhibitor FK506. Poster session presented at the Second Federation of European Biochemical Societies Advanced Lecture Course, La Colle sur Loup, France.
Conference Contribution - Verbal presentation and other Conference outputs
Cannon, R. D., Holmes, A. R., Lamping, E., Keniya, M. V., Niimi, K., & Monk, B. C. (2007, November). Overcoming Candida albicans drug resistance. Verbal presentation at the Joint New Zealand Microbiology Society and New Zealand Society for Biochemistry and Molecular Biology Annual Conference, Wellington, New Zealand.
2006
Journal - Research Article
Niimi, K., Maki, K., Ikeda, F., Holmes, A. R., Lamping, E., Niimi, M., Monk, B. C., & Cannon, R. D. (2006). Overexpression of Candida albicans CDR1, CDR2, or MDR1 does not produce significant changes in echinocandin susceptibility. Antimicrobial Agents & Chemotherapy, 50(4), 1148-1155.
Holmes, A. R., Tsao, S., Ong, S.-W., Lamping, E., Niimi, K., Monk, B. C., Niimi, M., … Cannon, R. D. (2006). Heterozygosity and functional allelic variation in the Candida albicans efflux pump genes CDR1 and CDR2. Molecular Microbiology, 62(1), 170-186.
Journal - Research Other
Holmes, A. R., Tsao, S., Lamping, E., Niimi, K., Monk, B. C., Tanabe, K., Niimi, M., & Cannon, R. D. (2006). Amino acid residues affecting drug pump function in Candida albicans: C. albicans drug pump function [Review]. Nippon Ishinkin Gakkai Zasshi, 47(4), 275-281.
Conference Contribution - Published proceedings: Abstract
Lamping, E., Nakamura, K., Ranchod, A., Holmes, A. R., Niimi, K., Niimi, M., & Cannon, R. D. (2006). Investigating the innate resistance of Candida krusei to azole antifungal drugs. Japanese Journal of Medical Mycology. 47(Suppl. 1), (pp. 67). [Abstract]
Niimi, K., Harding, D. R. K., Holmes, A. R., Lamping, E., Niimi, M., Cannon, R. D., & Monk, B. C. (2006). Identifying novel aspects of ABC transporter structure and function using surface-active chemosensitizers. Japanese Journal of Medical Mycology. 47(Suppl. 1), (pp. 67). [Abstract]
Conference Contribution - Poster Presentation (not in published proceedings)
Monk, B., Niimi, K., Lamping, E., Holmes, A., Cannon, R., Harding, D., & Niimi, M. (2006, March). Novel aspects of ABC transporter structure and function identified using a surface-active chemosensitiser. Poster session presented at the Federation of European Biochemical Societies Special Meeting, Innsbruck, Austria.
Niimi, K., Harding, D., Lamping, E., Holmes, A., Niimi, M., Cannon, R., & Monk, B. (2006, June). Identification and characterization of a drug efflux pump inhibitor specific to Candida albicans ABC transporter Cdr1p. Poster session presented at the 16th Congress of the International Society for Human and Animal Mycology, Paris, France.
Niimi, K., Maki, K., Ikeda, F., Holmes, A., Lamping, E., Niimi, M., Monk, B., & Cannon, R. (2006, June). Over-expression of cdr1, cdr2 or mdr1 in Candida albicans does not cause significant changes in candin susceptibility. Poster session presented at the 16th Congress of the International Society for Human and Animal Mycology, Paris, France.
Conference Contribution - Verbal presentation and other Conference outputs
Lamping, E., & Miyajima, T. (2006, November). Investigating the innate resistance of Candida krusei to azole antifungal drugs. Verbal presentation at the 3rd Molecular Fungal Biology Conference, Chiba, Japan.
Lamping, E., Niimi, K., Holmes, A. R., Monk, B., Tanabe, K., Niimi, M., & Cannon, R. D. (2006, November). Studying drug resistance mechanisms of pathogenic fungi. Verbal presentation at the 3rd Molecular Fungal Biology Conference, Chiba, Japan.
Cannon, R. D., Lamping, E., Holmes, A. R., Niimi, K., Niimi, M., & Monk, B. C. (2006, August). Overcoming the efflux-mediated drug resistance of human fungal pathogens. Verbal presentation at the 8th International Mycological Congress, Cairns, Australia.
2005
Journal - Research Article
Lamping, E., Tanabe, K., Niimi, M., Uehara, Y., Monk, B. C., & Cannon, R. D. (2005). Characterization of the Saccharomyces cerevisiae sec6-41 mutation and tools to create S. cerevisiae strains containing the sec6-4 allele. Gene, 361, 57-66.
Niimi, M., Tanabe, K., Wada, S.-I., Yamazaki, A., Uehara, Y., Niimi, K., Lamping, E., Holmes, A. R., Monk, B. C., & Cannon, R. D. (2005). ABC transporters of pathogenic fungi: Recent advances in functional analyses. Nippon Ishinkin Gakkai Zasshi, 46(4), 249-260.
Wada, S.-I., Tanabe, K., Yamazaki, A., Niimi, M., Uehara, Y., Niimi, K., Lamping, E., Cannon, R. D., & Monk, B. C. (2005). Phosphorylation of Candida glabrata ATP-binding cassette transporter Cdr1p regulates drug efflux activity and ATPase stability. Journal of Biological Chemistry, 280(1), 94-103.
Niimi, M., Wada, S.-I., Tanabe, K., Kaneko, A., Takano, Y., Umeyama, T., … Lamping, E., Niimi, K., Tsao, S., Holmes, A. R., Monk, B. C., & Cannon, R. D. (2005). Functional analysis of fungal drug efflux transporters by heterologous expression in Saccharomyces cerevisiae. Japanese Journal of Infectious Diseases, 58, 1-7.
Conference Contribution - Poster Presentation (not in published proceedings)
Niimi, K., Maki, K., Ikeda, F., Holmes, A. R., Lamping, E., Niimi, M., Monk, B. C., & Cannon, R. D. (2005, October). Micafungin is not an efflux pump substrate. Poster session presented at the 49th Annual Meeting of the Japanese Society for Medical Mycology, Chiba, Japan.
Lamping, E., Holmes, A. R., Niimi, K., Monk, B. C., Nakamura, K., Niimi, M., & Cannon, R. D. (2005, November). A system for heterologous over-expression of functional membrane proteins in Saccharomyces cerevisiae. Poster session presented at the Proteomics Satellite Meeting, Dunedin, New Zealand.
Niimi, K., Holmes, A. R., Lamping, E., Maki, K., Ikeda, F., Niimi, M., Monk, B. C., & Cannon, R. D. (2005, November). Are candins substrates of fungal drug efflux pumps? Poster session presented at the Joint Meeting of the New Zealand Microbiological Society and the New Zealand Society for Biochemistry and Molecular Biology, Dunedin, New Zealand.
Lamping, E., Nakamura, K., Ranchod, A., Holmes, A., Niimi, K., Monk, B., … Niimi, M., & Cannon, R. (2005, May). Genetic tools for the functional hyper-expression of fungal membrane proteins in Saccharomyces cerevisiae. Poster session presented at the Federation of European Biochemical Societies Advanced Lecture Course: Human Fungal Pathogens, La Colle-sur-Loup, France.
Conference Contribution - Verbal presentation and other Conference outputs
Monk, B. C., Niimi, K., Lamping, E., Holmes, A. R., Harding, D. R. K., Niimi, M., & Cannon, R. D. (2005, November). Identifying novel aspects of ABC transporter structure and function using surface-active chemosensitisers. Verbal presentation at the Joint Meeting of the New Zealand Microbiological Society and the New Zealand Society for Biochemistry and Molecular Biology, Dunedin, New Zealand.
Holmes, A. R., Tsao, S., Niimi, K., Lamping, E., Niimi, M., . . ., Monk, B. C., & Cannon, R. D. (2005, November). Allelic variation in fungal drug pumps. Verbal presentation at the Joint Meeting of the New Zealand Microbiological Society and the New Zealand Society for Biochemistry and Molecular Biology, Dunedin, New Zealand.
Monk, B. C., Niimi, K., Lamping, E., Holmes, A. R., Harding, D. R. K., Niimi, M., & Cannon, R. D. (2005, November). A Saccharomyces cerevisiae system for the heterologous hyper-expression of functional membrane proteins. Verbal presentation at the Proteomics Satellite Meeting, Dunedin, New Zealand.
Lamping, E., Nakamura, K., Holmes, A. R., Niimi, K., Monk, B. C., Niimi, M., & Cannon, R. D. (2005, November). A novel, versatile and highly efficient heterologous membrane protein over-expression system. Verbal presentation at the Joint Meeting of the New Zealand Microbiological Society and the New Zealand Society for Biochemistry and Molecular Biology, Dunedin, New Zealand.
Cannon, R. D., Tsao, S., Niimi, K., Ong, S.-W., Lamping, E., Monk, B. C., & Holmes, A. R. (2005, September). Efflux pump-mediated drug resistance in oral fungal pathogens. Verbal presentation at the 45th Annual Meeting of the Australian/New Zealand Division of the International Association for Dental Research, Queenstown, New Zealand.
2004
Conference Contribution - Poster Presentation (not in published proceedings)
Tanabe, K., Wada, S., Yamazaki, A., Niimi, K., Lamping, E., Cannon, R., Monk, B., … Niimi, M. (2004, December). Phosphorylation of Candida glabrata ATP-binding casselle transporter Cdr1p regulates drug-efflux activity and ATPase stability. Poster session presented at the 27th Annual Meeting of the Molecular Biology Society of Japan, Kobe, Japan.
Tanabe, K., Wada, S., Yamazaki, A., Niimi, M., Uehara, Y., Niimi, K., Lamping, E., Cannon, R. D., & Monk, B. C. (2004, September). Phosphorylation of Candida glabrata ABC transporter Cdr1p regulates drug efflux and ATPase activities. Poster session presented at the 27th Annual Meeting of the Japanese Society for Molecular Microbiology: Yeast Genetics Forum, Shimane, Japan.
Lamping, E., Monk, B. C., Niimi, M., & Cannon, R. D. (2004, September). Genetic tools for functional hyper-expression of membrane proteins in Saccharomyces cerevisiae. Poster session presented at the 27th Annual Meeting of the Japanese Society for Molecular Microbiology: Yeast Genetics Forum, Shimane, Japan.
Cannon, R. D., Tsao, S., Ong, S.-W., Niimi, K., Lamping, E., Niimi, M., Monk, B. C., & Holmes, A. R. (2004, March). Allelic variation effecting drug pump function in Candida albicans. Poster session presented at the 7th American Society for Microbiology Conference on Candida and candidiasis, Austin, Texas.
Conference Contribution - Verbal presentation and other Conference outputs
Lamping, E., Cannon, R. D., Niimi, K., Tsao, S., Holmes, A. R., Monk, B. C., & ... (2004, August). Drug resistance mechanisms in fungal pathogens-special emphasis on Candida albicans and Cryptococcus neoformans [Invited]. Verbal presentation at the Nippon Dental University, Niigata, Japan.
Holmes, A. R., Tsao, S., Niimi, K., Lamping, E., Niimi, M., Kaneko, A., Monk, B. C., & Cannon, R. D. (2004, November). Amino acid residues critical for drug pump function in Candida albicans. Verbal presentation at the New Zealand Microbiological Society Conference, Palmerston North, New Zealand.
Ranchod, A., Cannon, R. D., & Lamping, E. (2004, September). Efflux pump-mediated drug resistance in Candida krusei. Verbal presentation at the 44th Annual Meeting of the International Association for Dental Research, Australian and New Zealand Division, Nadi, Fiji.
Lamping, E., Nakamura, K., Monk, B. C., Holmes, A. R., Niimi, K., Tsao, S., Niimi, M., & Cannon, R. D. (2004, September). A membrane protein hyper-expression system and its application in the search for new antifungal drugs. Verbal presentation at the 48th Annual Meeting of the Japanese Society for Medical Mycology, Yokohama, Japan.
2003
Conference Contribution - Published proceedings: Abstract
Niimi, K., Lamping, E., Holmes, A. R., Monk, B. C., Cannon, R. D., & Niimi, M. (2003). Drug resistance mechanisms in pathogenic fungi. Journal of Japanese Society for Clinical Microbiology, 13(4), (pp. 51). [Abstract]
Ong, S.-W., Tsao, S., Holmes, A. R., Lamping, E., & Cannon, R. D. (2003). Allelic variation of the Candida albicans drug efflux pump Cdr1p. Journal of Dental Research, 82(Spec Iss C), (pp. C108). [Abstract]
Hull, C. H., Holmes, A. R., Niimi, K., Lamping, E., Monk, B. C., & Cannon, R. D. (2003). Chemosensitisation of multidrug resistant yeast to fluconazole. Journal of Dental Research, 82, (pp. C-78). [Abstract]
Conference Contribution - Poster Presentation (not in published proceedings)
Tsao, S., Holmes, A., Ong, S.-W., Lamping, E., Monk, B., Niimi, K., & Cannon, R. (2003, September). Allelic variations in a Candida albicans drug pump. Poster session presented at the MicroNZ: A Combined Annual Scientific Meeting & Exhibition of the Australian and New Zealand Societies for Microbiology, Auckland, New Zealand.
Monk, B. C., Niimi, K., Lamping, E., Holmes, A. R., Cannon, R. D., Harding, D. R. K., … Niimi, M., & Uehara, Y. (2003, July). Functional hyper-expression of membrane proteins in yeast and discovery of chemosensitizers of fungal multidrug efflux. Poster session presented at the XXIst International Conference on Yeast Genetics and Molecular Biology, Gothenburg, Sweden.
Conference Contribution - Verbal presentation and other Conference outputs
Monk, B. C., Niimi, K., Lamping, E., Holmes, A. R., Cannon, R. D., Harding, D. R. K., … Niimi, M., & Uehara, Y. (2003, September). Functional hyper-expression of membrane proteins in yeast and discovery of chemosensitizers of fungal multidrug efflux. Verbal presentation at the EuroConference on Host-Pathogen Interactions: Human fungal pathogens, Giens, France.
Monk, B. C., Niimi, K., Lamping, E., Holmes, A. R., Cannon, R. D., Harding, D. R. K., … Niimi, M., & Uehara, Y. (2003, January). Functional hyper-expression of membrane proteins and discovery of chemosensitizers of fungal multidrug efflux. Verbal presentation at the Symposium on 'ABC Proteins' and the 5th Conference on 'ABC Proteins and Ion Channels': From gene to disease, Kyoto, Japan.
Monk, B. C., Niimi, K., Lamping, E., Holmes, A. R., Cannon, R. D., Harding, D. R. K., … Niimi, M., & Uehara, Y. (2003, August). Functional hyper-expression of membrane proteins in yeast and discovery of chemosensitizers of fungal multidrug efflux. Verbal presentation at the 21st Small Meeting on Yeast Transport and Energetics, Bonn, Germany.
2002
Conference Contribution - Poster Presentation (not in published proceedings)
Hull, C., Homes, A. R., Niimi, K., Lamping, E., Monk, B. C., & Cannon, R. D. (2002, September). Chemosensitisation of multidrug resistant yeast top Fluconazole. Poster session presented at the 42nd Annual Scientific Meeting of the International Association for Dental Research (ANZ Division), Sydney, Australia.
Homes, A. R., Niimi, K., Lamping, E., Monk, B. C., & Cannon, R. D. (2002, November). Do SNP's affect multidrug resistance in the fungal pathogen Candida albicans? Poster session presented at the New Zealand Microbiological Society Microbes and Molecules Conference, Christchurch, New Zealand.
Intellectual Property
Monk, B. C., Cannon, R. D., Nakamura, K., Niimi, M., Niimi, K., Harding, D. R. K., Holmes, A. R., Lamping, E., … Decottignies, A. (2002). Membrane protein expression system and its application (International patent). International patent #PCT/NZ02/00163 (filed 24 August 2002). International Patent Office.
Monk, B. C., Nakamura, K., Cannon, R. D., Niimi, M., Niimi, K., Harding, D. R. K., Holmes, A. R., Lamping, E., … Decottignies, A. (2002). Membrane protein expression system and its application (NZ patent). New Zealand provisional patent #513755 (filed 24 August 2001). Intellectual Property Office of New Zealand.