Dr Adele Woolley
| Position | Senior Lecturer |
|---|---|
| Department | Department of Pathology (Dunedin) |
| Qualifications | BPhEd BSc PGDipSci PhD(Otago) |
| Research summary | Molecular mechanisms underlying cancer, with a particular focus on aberrant expression of developmental pathways in cancer progression |
| Teaching |
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| Memberships |
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Research
The main focus of my research is the investigation into the role of the oncoprotein YB-1 in cancer, with a particular focus on breast cancer. As a developmental biologist I am particularly interested in aberrations in developmental pathways underpinning cell cycle regulation in relation to cancer progression. Specific threads of research contributing to these investigations specifically investigate the role of YB-1 in the regulation of:
- phosphatases such as DUSP4
- specific miRNAs (in a tissue dependent manner)
- the p53-binding protein (PACT-1)
- EMT
Techniques used in these investigations include:
- cell culture
- gene knockdown and overexpression
- quantitative RT-PCR
- protein analysisby western blot
- Microscopy; brightfield, immunofluorescent, confocal, correlative
Model systems used are:
- Human cancer cell lines
- Zebrafish (Danio rerio)
I have a number of other research interests including the role of BRCA1 in ovarian cancer and genes regulating invasiveness in melanoma.
Publications
Mehta, S., McKinney, C., Algie, M., Verma, C. S., Kannan, S., Harfoot, R., … Gould, M. L., Parker, K., … Cunliffe, H. E., … Kleffmann, T., Braithwaite, A. W., & Woolley, A. G. (2020). Dephosphorylation of YB-1 is required for nuclear localisation during G2 phase of the cell cycle. Cancers, 12(2), 315. doi: 10.3390/cancers12020315
Roth, I., Campbell, H., Rubio, C., Vennin, C., Wilson, M., Wiles, A., Williams, G., Woolley, A., … Fleming, N., Baird, M., & Braithwaite, A. W. (2016). The Δ133p53 isoform and its mouse analogue Δ122p53 promote invasion and metastasis involving pro-inflammatory molecules interleukin-6 and CCL2. Oncogene, 35, 4981-4989. doi: 10.1038/onc.2016.45
Lasham, A., Mehta, S. Y., Fitzgerald, S. J., Woolley, A. G., Hearn, J. I., Hurley, D. G., Ruza, I., Algie, M., … Braithwaite, A. W., & Print, C. G. (2016). A novel EGR-1 dependent mechanism for YB-1 modulation of paclitaxel response in a triple negative breast cancer cell line. International Journal of Cancer, 139(5), 1157-1170. doi: 10.1002/ijc.30137
Fleming, J. S., Ruza, I., Thompson, B. A., Lee, K.-F., & Woolley, A. G. (2015). Effects of BRCA1 knockdown on CYP19a1/aromatase and steroid receptor expression in ovarian and tubal cells. Journal of Molecular & Genetic Medicine, 9(4), 185. doi: 10.4172/1747-0862.1000185
Lasham, A., Print, C. G., Woolley, A. G., Dunn, S. E., & Braithwaite, A. W. (2013). YB-1: Oncoprotein, prognostic marker and therapeutic target? Biochemical Journal, 449, 11-23. doi: 10.1042/bj20121323
Journal - Research Article
Mehta, S., McKinney, C., Algie, M., Verma, C. S., Kannan, S., Harfoot, R., … Gould, M. L., Parker, K., … Cunliffe, H. E., … Kleffmann, T., Braithwaite, A. W., & Woolley, A. G. (2020). Dephosphorylation of YB-1 is required for nuclear localisation during G2 phase of the cell cycle. Cancers, 12(2), 315. doi: 10.3390/cancers12020315
Roth, I., Campbell, H., Rubio, C., Vennin, C., Wilson, M., Wiles, A., Williams, G., Woolley, A., … Fleming, N., Baird, M., & Braithwaite, A. W. (2016). The Δ133p53 isoform and its mouse analogue Δ122p53 promote invasion and metastasis involving pro-inflammatory molecules interleukin-6 and CCL2. Oncogene, 35, 4981-4989. doi: 10.1038/onc.2016.45
Lasham, A., Mehta, S. Y., Fitzgerald, S. J., Woolley, A. G., Hearn, J. I., Hurley, D. G., Ruza, I., Algie, M., … Braithwaite, A. W., & Print, C. G. (2016). A novel EGR-1 dependent mechanism for YB-1 modulation of paclitaxel response in a triple negative breast cancer cell line. International Journal of Cancer, 139(5), 1157-1170. doi: 10.1002/ijc.30137
Fleming, J. S., Ruza, I., Thompson, B. A., Lee, K.-F., & Woolley, A. G. (2015). Effects of BRCA1 knockdown on CYP19a1/aromatase and steroid receptor expression in ovarian and tubal cells. Journal of Molecular & Genetic Medicine, 9(4), 185. doi: 10.4172/1747-0862.1000185
Lasham, A., Print, C. G., Woolley, A. G., Dunn, S. E., & Braithwaite, A. W. (2013). YB-1: Oncoprotein, prognostic marker and therapeutic target? Biochemical Journal, 449, 11-23. doi: 10.1042/bj20121323
Awasthi, A., Woolley, A. G., Lecomte, F. J., Hung, N., Baguley, B. C., Wilbanks, S. M., Jeffs, A. R., & Tyndall, J. D. A. (2013). Variable expression of GLIPR1 correlates with invasive potential in melanoma cells. Frontiers in Oncology, 3, 225. doi: 10.3389/fonc.2013.00225
Lasham, A., Samuel, W., Cao, H., Patel, R., Mehta, R., Stern, J. L., … Woolley, A. G., … Black, M. A., Shelling, A. N., Print, C. G., & Braithwaite, A. W. (2012). YB-1, the E2F Pathway, and regulation of tumor cell growth. Journal of the National Cancer Institute, 104(2), 133-146. doi: 10.1093/jnci/djr512
Woolley, A. G., Algie, M., Samuel, W., Harfoot, R., Wiles, A., Hung, N. A., … Royds, J. A., Perez, D., Yoon, H.-S., … Braithwaite, A. W. (2011). Prognostic association of YB-1 expression in breast cancers: A matter of antibody. PLoS ONE, 6(6), e20603. doi: 10.1371/journal.pone.0020603
Sheard, P. W., Bewick, G. S., Woolley, A. G., Shaw, J., Fisher, L., Fong, S. W., & Duxson, M. J. (2010). Investigation of neuromuscular abnormalities in neurotrophin-3-deficient mice. European Journal of Neuroscience, 31(1), 29-41. doi: 10.1111/j.1460-9568.2009.07032.x
Cohen, S. B., Ma, W., Valova, V. A., Algie, M., Harfoot, R., Woolley, A. G., … Braithwaite, A. W. (2010). Genotoxic stress-induced nuclear localization of oncoprotein YB-1 in the absence of proteolytic processing. Oncogene, 29, 403-410. doi: 10.1038/onc.2009.321
Jeffs, A. R., Glover, A. C., Slobbe, L. J., Wang, L., He, S., Hazlett, J. A., Awasthi, A., Woolley, A. G., … Print, C. G., … Eccles, M. R. (2009). A gene expression signature of invasive potential in metastatic melanoma cells. PLoS ONE, 4(12), e8461. doi: 10.1371/journal.pone.0008461
Woolley, A. G., Tait, K. J., Hurren, B. J., Fisher, L., Sheard, P. W., & Duxson, M. J. (2008). Developmental loss of NT-3 in vivo results in reduced levels of myelin-specific proteins, a reduced extent of myelination and increased apoptosis of Schwann cells. GLIA, 56(3), 306-317. doi: 10.1002/glia.20614
Woolley, A. G., Sheard, P. W., & Duxson, M. J. (2005). Neurotrophin-3 null mutant mice display a postnatal motor neuropathy. European Journal of Neuroscience, 21, 2100-2110.
Woolley, A., Sheard, P. W., Dodds, K. G., & Duxson, M. J. (1999). Alpha motoneurons are present in normal numbers but with reduced soma size in neurotrophin-3 knockout mice. Neuroscience Letters, 272, 107-110.
