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Professor Mark Hampton

Mark HamptonPrincipal Investigator

PhD (Otago) MSc(Hons) (Canterbury)

Email mark.hampton@otago.ac.nz
Tel +64 3 378 6225

Mark Hampton is a professor at the Department of Pathology and Biomedical Science on the University of Otago, Christchurch campus, and director of the Centre for Free Radical Research.

He completed his undergraduate and PhD studies in New Zealand before taking up postdoctoral positions at the Karolinksa Institute in Sweden and Harvard in the USA. He subsequently returned to the University of Otago where he was promoted to a professorial role in 2013.

Research interests

Oxidants (also called reactive oxygen species or free radicals) are continuously generated in our body, and we rely on sophisticated antioxidant systems to prevent them from causing damage. However, oxidants also have beneficial roles. They can act as signalling molecules that regulate a number of life and death pathways in cells, and they are generated by the immune system to fight microbes. Prof Hampton's research is focused on how understanding how cells sense and respond to oxidants, and how these pathways might be modulated to prevent and treat human disease.

In the early stages of Professor Hampton's career he investigated the role that neutrophil oxidants play in killing phagocytosed microbes. His interests then broadened to the impact of these oxidants on neighbouring cells during inflammation, and the controlled generation of oxidants by other cells during normal metabolism.

Professor Hampton's current projects include investigations of the ability of oxidants generated by the innate immune system to regulate epigenetic and cell death pathways; how the disruption of redox homeostasis can promote the killing of cancer cells and pathogenic microbes; and how oxidative stress is associated with the biological processes underlying human ageing.

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Publications

Smyth, L. C. D., Murray, H. C., Hill, M., van Leeuwen, E., Highet, B., Magon, N. J., … Mathiesen, S. N., Mockett, B., … Clarkson, A. N., … Abraham, W. C., Hughes, S. M., … Kettle, A. J., … Hampton, M. B. (2022). Neutrophil-vascular interactions drive myeloperoxidase accumulation in the brain in Alzheimer's disease. Acta Neuropathologicca Communications, 10, 38. doi: 10.1186/s40478-022-01347-2

van Leeuwen, E., Hampton, M. B., & Smyth, L. C. D. (2022). Hypothiocyanous acid disrupts the barrier function of brain endothelial cells. Antioxidants, 11, 608. doi: 10.3390/antiox11040608

Shearer, H. L., Kaldor, C. D., Hua, H., Kettle, A. J., Parker, H. A., & Hampton, M. B. (2022). Resistance of Streptococcus pneumoniae to hypothiocyanous acid generated by host peroxidases. Infection & Immunity. Advance online publication. doi: 10.1128/iai.00530-21

Shearer, H. L., Paton, J. C., Hampton, M. B., & Dickerhof, N. (2022). Glutathione utilization protects Streptococcus pneumoniae against lactoperoxidase-derived hypothiocyanous acid. Free Radical Biology & Medicine, 179, 24-33. doi: 10.1016/j.freeradbiomed.2021.12.261

Shearer, H. L., Hampton, M. B., & Dickerhof, N. (2022). Bactericidal activity of the oxidants derived from mammilian heme peroxidases. In C. L. Hawkins & W. M. Nauseef (Eds.), Mammalian heme peroxidases: Diverse roles in health and disease. (pp. 171-188). Boca Raton, FL: CRC Press. doi: 10.1201/9781003212287

Vissers, M. C. M., Hampton, M. B., & Kettle, A. J. (Eds.). (2018). Hydrogen peroxide metabolism in health and disease. Boca Raton, FL: CRC Press, 456p.

Edited Book - Research

Shearer, H. L., Hampton, M. B., & Dickerhof, N. (2022). Bactericidal activity of the oxidants derived from mammilian heme peroxidases. In C. L. Hawkins & W. M. Nauseef (Eds.), Mammalian heme peroxidases: Diverse roles in health and disease. (pp. 171-188). Boca Raton, FL: CRC Press. doi: 10.1201/9781003212287

Chapter in Book - Research

Magon, N. J., Parker, H. A., Ashby, L. V., Springer, R. J., & Hampton, M. B. (2020). Analysis of neutrophil bactericidal activity. In M. T. Quinn & F. R. DeLeo (Eds.), Neutrophil: Methods and protocols: Methods in molecular biology (Vol. 2087). (3rd ed.) (pp. 149-164). New York, NY: Humana Press. doi: 10.1007/978-1-0716-0154-9_12

Chapter in Book - Research

Hampton, M. B. (2018). Hydrogen peroxide-dependent redox signaling: Basic concepts and unanswered questions. In M. C. M. Vissers, M. B. Hampton & A. J. Kettle (Eds.), Hydrogen peroxide metabolism in health and disease. (pp. 353-364). Boca Raton, FL: CRC Press.

Chapter in Book - Research

Parker, H. A., Magon, N. J., Green, J. N., Hampton, M. B., & Winterbourn, C. C. (2014). Analysis of neutrophil bactericidal activity. In M. T. Quinn & F. R. DeLeo (Eds.), Neutrophil methods and protocols: Methods in Molecular Biology (Vol. 1124). (2nd ed.) (pp. 291-306). New York, NY: Springer. doi: 10.1007/978-1-62703-845-4_19

Chapter in Book - Research

Cox, A. G., Winterbourn, C. C., & Hampton, M. B. (2010). Measuring the redox state of cellular peroxiredoxins by immunoblotting. In E. Cadenas & L. Packer (Eds.), Methods in enzymology: Thiol redox transitions in cell signaling: Part B: Cellular localization and signaling (Vol. 474). (pp. 51-66). San Diego, CA: Academic Press. doi: 10.1016/S0076-6879(10)74004-0

Chapter in Book - Research

Requejo, R., Chouchani, E. T., Hurd, T. R., Menger, K. E., Hampton, M. B., & Murphy, M. P. (2010). Measuring mitochondrial protein thiol redox state. In E. Cadenas & L. Packer (Eds.), Methods in enzymology: Thiol redox transitions in cell signaling: Part B: Cellular localization and signaling (Vol. 474). (pp. 123-147). San Diego, CA: Academic Press. doi: 10.1016/S0076-6879(10)74004-0

Chapter in Book - Research

Green, J. N., Winterbourn, C. C., & Hampton, M. B. (2007). Analysis of neutrophil bactericidal activity. In M. T. Quinn, F. R. DeLeo & G. M. Bokoch (Eds.), Neutrophil methods and protocols. (pp. 319-332). Totowa, USA: Humana Press.

Chapter in Book - Research

Hampton, M. B., Baty, J. W., & Winterbourn, C. C. (2006). Use of a proteomic technique to identify oxidant-sensitive thiol proteins in cultured cells. In I. Dalle-Donne, A. Scaloni & D. A. Butterfield (Eds.), Redox Proteomics: From protein modifications to cellular dysfunction and diseases. (pp. 253-265). Hoboken, NJ: John Wiley & Sons.

Chapter in Book - Research

Winterbourn, C. C., van Dalen, C. J., Hampton, M. B., & Kettle, A. J. (2000). Reactions of myeloperoxidase and production of hypochlorous acid in neutrophil phagosomes. In P. E. Petrides & W. M. Nauseef (Eds.), The Peroxidase Multigene Family of Enzymes. (pp. 58-67). Berlin: Springer.

Chapter in Book - Research

Shearer, H. L., Kaldor, C. D., Hua, H., Kettle, A. J., Parker, H. A., & Hampton, M. B. (2022). Resistance of Streptococcus pneumoniae to hypothiocyanous acid generated by host peroxidases. Infection & Immunity. Advance online publication. doi: 10.1128/iai.00530-21

Journal - Research Article

Shearer, H. L., Paton, J. C., Hampton, M. B., & Dickerhof, N. (2022). Glutathione utilization protects Streptococcus pneumoniae against lactoperoxidase-derived hypothiocyanous acid. Free Radical Biology & Medicine, 179, 24-33. doi: 10.1016/j.freeradbiomed.2021.12.261

Journal - Research Article

Smyth, L. C. D., Murray, H. C., Hill, M., van Leeuwen, E., Highet, B., Magon, N. J., … Mathiesen, S. N., Mockett, B., … Clarkson, A. N., … Abraham, W. C., Hughes, S. M., … Kettle, A. J., … Hampton, M. B. (2022). Neutrophil-vascular interactions drive myeloperoxidase accumulation in the brain in Alzheimer's disease. Acta Neuropathologicca Communications, 10, 38. doi: 10.1186/s40478-022-01347-2

Journal - Research Article

van Leeuwen, E., Hampton, M. B., & Smyth, L. C. D. (2022). Hypothiocyanous acid disrupts the barrier function of brain endothelial cells. Antioxidants, 11, 608. doi: 10.3390/antiox11040608

Journal - Research Article

Das, A. B., Seddon, A. R., O'Connor, K. M., & Hampton, M. B. (2021). Regulation of the epigenetic landscape by immune cell oxidants. Free Radical Biology & Medicine. Advance online publication. doi: 10.1016/j.freeradbiomed.2020.12.453

Journal - Research Article

Königstorfer, A., Ashby, L. V., Bollar, G. E., Billiot, C. E., Gray, M. J., Jakob, U., Hampton, M. B., & Winterbourn, C. C. (2021). Induction of the reactive chlorine-responsive transcription factor RclR in Escherichia coli following ingestion by neutrophils. Pathogens & Disease, 79, ftaa079. doi: 10.1093/femspd/ftaa079

Journal - Research Article

Mayyas, I. M., Weeks, R. J., Day, R. C., Magrath, H. E., O’Connor, K. M., Kardailsky, O., Hore, T. A., Hampton, M. B., & Morison, I. M. (2021). Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation. Epigenetics, 16(11), 1251-1259. doi: 10.1080/15592294.2020.1861169

Journal - Research Article

Parker, H. A., Dickerhof, N., Forrester, L., Ryburn, H., Smyth, L., Messens, J., Aung, H. L., Cook, G. M., Kettle, A. J., & Hampton, M. B. (2021). Mycobacterium smegmatis resists the bactericidal activity of hypochlorous acid produced in neutrophil phagosomes. Journal of Immunology, 206, 1901-1912. doi: 10.4049/jimmunol.2001084

Journal - Research Article

Parker, H. A., Jones, H. M., Kaldor, C. D., Hampton, M. B., & Winterbourn, C. C. (2021). Neutrophil NET formation with microbial stimuli requires late stage NADPH oxidase activity. Antioxidants, 10, 1791. doi: 10.3390/antiox10111791

Journal - Research Article

Pearson, A. G., Pullar, J. M., Cook, J., Spencer, E. S., Vissers, M. C. M., Carr, A. C., & Hampton, M. B. (2021). Peroxiredoxin 2 oxidation reveals hydrogen peroxide generation within erythrocytes during high-dose vitamin C administration. Redox Biology, 43, 101980. doi: 10.1016/j.redox.2021.101980

Journal - Research Article

Schindler, L., Smyth, L. C. D., Bernhagen, J., Hampton, M. B., & Dickerhof, N. (2021). Macrophage migration inhibitory factor (MIF) enhances hypochlorous acid production in phagocytic neutrophils. Redox Biology, 41, 101946. doi: 10.1016/j.redox.2021.101946

Journal - Research Article

Schindler, L., Zwissler, L., Krammer, C., Hendgen-Cotta, U., Rassaf, T., Hampton, M. B., Dickerhof, N., & Bernhagen, J. (2021). Macrophage migration inhibitory factor inhibits neutrophil apoptosis by inducing cytokine release from mononuclear cells. Journal of Leukocyte Biology. Advance online publication. doi: 10.1002/JLB.3A0420-242RRR

Journal - Research Article

Seddon, A. R., Liau, Y., Pace, P. E., Miller, A. L., Das, A. B., Kennedy, M. A., Hampton, M. B., & Stevens, A. J. (2021). Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells. Epigenetics & Chromatin, 14(1), 17. doi: 10.1186/s13072-021-00388-6

Journal - Research Article

Smith-Díaz, C. C., Magon, N. J., McKenzie, J. L., Hampton, M. B., Vissers, M. C. M., & Das, A. B. (2021). Ascorbate inhibits proliferation and promotes myeloid differentiation in TP53-mutant leukemia. Frontiers in Oncology, 11, 709543. doi: 10.3389/fonc.2021.709543

Journal - Research Article

Meng, Y., Sheen, C. R., Magon, N. J., Hampton, M. B., & Dobson, R. C. J. (2020). Structure-function analyses of alkylhydroperoxidase D from Streptococcus pneumoniae reveal an unusual three-cysteine active site architecture. Journal of Biological Chemistry, 295(10), 2984-2999. doi: 10.1074/jbc.RA119.012226

Journal - Research Article

O'Connor, K. M., Das, A. B., Winterbourn, C. C., & Hampton, M. B. (2020). Inhibition of DNA methylation in proliferating human lymphoma cells by immune cell oxidants. Journal of Biological Chemistry, 295(23), 7839-7848. doi: 10.1074/jbc.RA120.013092

Journal - Research Article

Pearson, A. G., Zawari, M., Pearson, J. F., & Hampton, M. B. (2020). Quantifying mitochondrial respiration in human lymphocytes and monocytes challenged with hydrogen peroxide. Free Radical Research, 54(4), 271-279. doi: 10.1080/10715762.2020.1753722

Journal - Research Article

Van Leeuwen, E., Hampton, M. B., & Smyth, L. C. D. (2020). Redox signalling and regulation of the blood-brain barrier. International Journal of Biochemistry & Cell Biology, 125, 105794. doi: 10.1016/j.biocel.2020.105794

Journal - Research Article

de Souza, L. F., Pearson, A. G., Pace, P. E., Dafre, A. L., Hampton, M. B., Meotti, F. C., & Winterbourn, C. C. (2019). Peroxiredoxin expression and redox status in neutrophils and HL-60 cells. Free Radical Biology & Medicine, 135, 227-234. doi: 10.1016/j.freeradbiomed.2019.03.007

Journal - Research Article

Dickerhof, N., Isles, V., Pattemore, P., Hampton, M. B., & Kettle, A. J. (2019). Exposure of Pseudomonas aeruginosa to bactericidal hypochlorous acid during neutrophil phagocytosis is compromised in cystic fibrosis. Journal of Biological Chemistry, 294(36), 13502-13514. doi: 10.1074/jbc.RA119.009934

Journal - Research Article

Talla, U., Bozonet, S. M., Parker, H. A., Hampton, M. B., & Vissers, M. C. M. (2019). Prolonged exposure to hypoxia induces an autophagy-like cell survival program in human neutrophils. Journal of Leukocyte Biology, 106, 1367-1379. doi: 10.1002/jlb.4a0319-079rr

Journal - Research Article

Hampton, M. B., Vick, K. A., Skoko, J. J., & Neumann, C. A. (2018). Peroxiredoxin involvement in the initiation and progression of human cancer. Antioxidants & Redox Signaling, 28(7), 591-608. doi: 10.1089/ars.2017.7422

Journal - Research Article

Pace, P. E., Peskin, A. V., Konigstorfer, A., Jasoni, C. L., Winterbourn, C. C., & Hampton, M. B. (2018). Peroxiredoxin interaction with the cytoskeletal-regulatory protein CRMP2: Investigation of a putative redox relay. Free Radical Biology & Medicine, 129, 383-393. doi: 10.1016/j.freeradbiomed.2018.10.407

Journal - Research Article

Richards, S. J. G., Frizelle, F. A., Geddes, J. A., Eglinton, T. W., & Hampton, M. B. (2018). Frailty in surgical patients. International Journal of Colorectal Disease, 33(12), 1657-1666. doi: 10.1007/s00384-018-3163-y

Journal - Research Article

Yewdall, N. A., Peskin, A. V., Hampton, M. B., Goldstone, D. C., Pearce, F. G., & Gerrard, J. A. (2018). Quaternary structure influences the peroxidase activity of peroxiredoxin 3. Biochemical & Biophysical Research Communications, 497(2), 558-563. doi: 10.1016/j.bbrc.2018.02.093

Journal - Research Article

Dagnell, M., Pace, P. E., Cheng, Q., Frijhoff, J., Östman, A., Arnér, E. S. J., Hampton, M. B., & Winterbourn, C. C. (2017). Thioredoxin reductase 1 and NADPH directly protect protein tyrosine phosphatase 1B from inactivation during H2O2 exposure. Journal of Biological Chemistry, 292(35), 14371-14380. doi: 10.1074/jbc.M117.793745

Journal - Research Article

Schindler, L., Dickerhof, N., Hampton, M. B., & Bernhagen, J. (2017). Post-translational regulation of macrophage migration inhibitory factor: Basis for functional fine-tuning. Redox Biology, 15, 135-142. doi: 10.1016/j.redox.2017.11.028

Journal - Research Article

Bayer, S. B., Low, F. M., Hampton, M. B., & Winterbourn, C. C. (2016). Interactions between peroxiredoxin 2, hemichrome and the erythrocyte membrane. Free Radical Research, 50(12), 1329-1339. doi: 10.1080/10715762.2016.1241995

Journal - Research Article

Cuddihy, S. L., Drake, S., Harwood, T., Selwood, A. I., McNabb, P. S., & Hampton, M. B. (2016). The marine cytotoxin portimine is a potent and selective inducer of apoptosis. Apoptosis, 21(12), 1447-1452. doi: 10.1007/s10495-016-1302-x

Journal - Research Article

Poynton, R. A., Peskin, A. V., Haynes, A. C., Lowther, W. T., Hampton, M. B., & Winterbourn, C. C. (2016). Kinetic analysis of structural influences on the susceptibility of peroxiredoxins 2 and 3 to hyperoxidation. Biochemical Journal, 473, 411-421. doi: 10.1042/bj20150572

Journal - Research Article

Yewdall, N. A., Venugopal, H., Desfosses, A., Abrishami, V., Yosaatmadja, Y., Hampton, M. B., … Radjainia, M. (2016). Structures of human peroxiredoxin 3 suggest self-chaperoning assembly that maintains catalytic state. Structure, 24(7), 1120-1129. doi: 10.1016/j.str.2016.04.013

Journal - Research Article

Balasubramanain, D., Deng, A. X., Doudney, K., Hampton, M. B., & Kennedy, M. A. (2015). Valproic acid exposure leads to upregulation and increased promoter histone acetylation of sepiapterin reductase in a serotonergic cell line. Neuropharmacology, 99, 79-88. doi: 10.1016/j.neuropharm.2015.06.018

Journal - Research Article

Bayer, S. B., Hampton, M. B., & Winterbourn, C. C. (2015). Accumulation of oxidized peroxiredoxin 2 in red blood cells and its prevention. Transfusion, 55(8), 1909-1918. doi: 10.1111/trf.13039

Journal - Research Article

Dickerhof, N., Schindler, L., Bernhagen, J., Kettle, A. J., & Hampton, M. B. (2015). Macrophage migration inhibitory factor (MIF) Is rendered enzymatically inactive by myeloperoxidase-derived oxidants but retains its immunomodulatory function. Free Radical Biology & Medicine, 89, 498-511. doi: 10.1016/j.freeradbiomed.2015.09.009

Journal - Research Article

Jodczyk, S., Pearson, J. F., Aitchison, A., Miller, A. L., Hampton, M. B., & Kennedy, M. A. (2015). Telomere length measurement on the Roche LightCycler 480 platform. Genetic Testing & Molecular Biomarkers, 19(2), 63-68. doi: 10.1089/gtmb.2014.0208

Journal - Research Article

Magon, N. J., Turner, R., Gearry, R. B., Hampton, M. B., Sly, P. D., & Kettle, A. J. (2015). Oxidation of calprotectin by hypochlorous acid prevents chelation of essential metal ions and allows bacterial growth: Relevance to infections in cystic fibrosis. Free Radical Biology & Medicine, 86, 133-144. doi: 10.1016/j.freeradbiomed.2015.05.022

Journal - Research Article

Newman, T. A. C., Carleton, C. R., Leeke, B., Hampton, M. B., & Horsfield, J. A. (2015). Embryonic oxidative stress results in reproductive impairment for adult zebrafish. Redox Biology, 6, 648-655. doi: 10.1016/j.redox.2015.10.010

Journal - Research Article

Radjainia, M., Venugopal, H., Desfosses, A., Phillips, A. M., Yewdall, N. A., Hampton, M. B., … Mitra, A. K. (2015). Cryo-electron microscopy structure of human peroxiredoxin-3 filament reveals the assembly of a putative chaperone. Structure, 23(5), 912-920. doi: 10.1016/j.str.2015.03.019

Journal - Research Article

Spencer, E. S., Dale, E. J., Gommans, A. L., Rutledge, M. T., Vo, C. T., Nakatani, Y., Gamble, A. B., Smith, R. A. J., Wilbanks, S. M., Hampton, M. B., & Tyndall, J. D. A. (2015). Multiple binding modes of isothiocyanates that inhibit macrophage migration inhibitory factor. European Journal of Medicinal Chemistry, 93, 501-510. doi: 10.1016/j.ejmech.2015.02.012

Journal - Research Article

Wilkie-Grantham, R. P., Magon, N. J., Harwood, D. T., Kettle, A. J., Vissers, M. C., Winterbourn, C. C., & Hampton, M. B. (2015). Myeloperoxidase-dependent lipid peroxidation promotes the oxidative modification of cytosolic proteins in phagocytic neutrophils. Journal of Biological Chemistry, 290(15), 9896-9905. doi: 10.1074/jbc.M114.613422

Journal - Research Article

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