Research FellowResearch interests
Dr Louisa Ashby's research is focused on the biochemistry of the neutrophil, and in particular the enzymology of myeloperoxidase. This enzyme kills microbes by generating chlorine bleach, but during inflammation it can also damage healthy tissue.
She is also interested in the oxidation chemistry of myeloperoxidase in its microbicidal activity, and how to effectively inhibit the enzyme from causing oxidative stress in disease.
Publications
Königstorfer, A., Ashby, L. V., Bollar, G. E., Billiot, C. E., Gray, M. J., Jakob, U., Hampton, M. B., & Winterbourn, C. C. (2021). Induction of the reactive chlorine-responsive transcription factor RclR in Escherichia coli following ingestion by neutrophils. Pathogens & Disease, 79, ftaa079. doi: 10.1093/femspd/ftaa079
Magon, N. J., Parker, H. A., Ashby, L. V., Springer, R. J., & Hampton, M. B. (2020). Analysis of neutrophil bactericidal activity. In M. T. Quinn & F. R. DeLeo (Eds.), Neutrophil: Methods and protocols: Methods in molecular biology (Vol. 2087). (3rd ed.) (pp. 149-164). New York, NY: Humana Press. doi: 10.1007/978-1-0716-0154-9_12
Ashby, L. V., Springer, R., Hampton, M. B., Kettle, A. J., & Winterbourn, C. C. (2020). Evaluating the bactericidal action of hypochlorous acid in culture media [Short communication]. Free Radical Biology & Medicine. Advance online publication. doi: 10.1016/j.freeradbiomed.2020.07.033
Hsiao, K.-C., Caldwell, I., Woon, S. T., & Ashby, L. (2020). Novel heterozygous dominant activating RAC2 variant in four New Zealand patients with combined immunodeficiency. Journal of Clinical Immunology, 40(Suppl. 1), (pp. S120-S121). doi: 10.1007/s10875-020-00764-z
Forbes, L. V., & Kettle, A. J. (2018). Myeloperoxidase: Unleashing the power of hydrogen peroxide. In M. C. M. Vissers, M. B. Hampton & A. J. Kettle (Eds.), Hydrogen peroxide metabolism in health and disease. (pp. 281-304). Boca Raton, FL: CRC Press.
Chapter in Book - Research
Magon, N. J., Parker, H. A., Ashby, L. V., Springer, R. J., & Hampton, M. B. (2020). Analysis of neutrophil bactericidal activity. In M. T. Quinn & F. R. DeLeo (Eds.), Neutrophil: Methods and protocols: Methods in molecular biology (Vol. 2087). (3rd ed.) (pp. 149-164). New York, NY: Humana Press. doi: 10.1007/978-1-0716-0154-9_12
Forbes, L. V., & Kettle, A. J. (2018). Myeloperoxidase: Unleashing the power of hydrogen peroxide. In M. C. M. Vissers, M. B. Hampton & A. J. Kettle (Eds.), Hydrogen peroxide metabolism in health and disease. (pp. 281-304). Boca Raton, FL: CRC Press.
Journal - Research Article
Königstorfer, A., Ashby, L. V., Bollar, G. E., Billiot, C. E., Gray, M. J., Jakob, U., Hampton, M. B., & Winterbourn, C. C. (2021). Induction of the reactive chlorine-responsive transcription factor RclR in Escherichia coli following ingestion by neutrophils. Pathogens & Disease, 79, ftaa079. doi: 10.1093/femspd/ftaa079
Albrett, A. M., Ashby, L. V., Dickerhof, N., Kettle, A. J., & Winterbourn, C. C. (2018). Heterogeneity of hypochlorous acid production in individual neutrophil phagosomes revealed by a rhodamine-based probe. Journal of Biological Chemistry, 293(40), 15715-15724. doi: 10.1074/jbc.RA118.004789
Coker, M. S., Forbes, L. V., Plowman-Holmes, M., Murdoch, D. R., Winterbourn, C. C., & Kettle, A. J. (2018). Interactions of staphyloxanthin and enterobactin with myeloperoxidase and reactive chlorine species. Archives of Biochemistry & Biophysics, 646, 80-89. doi: 10.1016/j.abb.2018.03.039
Forbes, L. V., & Kettle, A. J. (2018). A multi-substrate assay for finding physiologically effective inhibitors of myeloperoxidase. Analytical Biochemistry, 544, 13-21. doi: 10.1016/j.ab.2017.12.022
Turner, R., Brennan, S. O., Ashby, L. V., Dickerhof, N., Hamzah, M. R., Pearson, J. F., Stamp, L. K., & Kettle, A. J. (2018). Conjugation of urate-derived electrophiles to proteins during normal metabolism and inflammation. Journal of Biological Chemistry, 293(51), 19886-19898. doi: 10.1074/jbc.RA118.005237
Stamp, L. K., Turner, R., Khalilova, I. S., Zhang, M., Drake, J., Forbes, L. V., & Kettle, A. J. (2014). Myeloperoxidase and oxidation of uric acid in gout: Implications for the clinical consequences of hyperuricaemia. Rheumatology, 53(11), 1958-1965. doi: 10.1093/rheumatology/keu218
Kettle, A. J., Albrett, A. M., Chapman, A. L., Dickerhof, N., Forbes, L. V., Khalilova, I., & Turner, R. (2014). Measuring chlorine bleach in biology and medicine. Biochimica et Biophysica Acta: General Subjects, 1840(2), 781-793. doi: 10.1016/j.bbagen.2013.07.004
Forbes, L. V., Sjögren, T., Auchère, F., Jenkins, D. W., Thong, B., Laughton, D., … Turner, R., … Kettle, A. J. (2013). Potent reversible inhibition of myeloperoxidase by aromatic hydromates. Journal of Biological Chemistry, 288(51), 36636-36647. doi: 10.1074/jbc.M113.507756
Forbes, L. V., Furtmüller, P. G., Khalilova, I., Turner, R., Obinger, C., & Kettle, A. J. (2012). Isoniazid as a substrate and inhibitor of myeloperoxidase: Identification of amine adducts and the influence of superoxide dismutase on their formation. Biochemical Pharmacology, 84(7), 949-960. doi: 10.1016/j.bcp.2012.07.020
Tidén, A.-K., Sjögren, T., Svensson, M., Bernlind, A., Senthilmohan, R., Auchère, F., … Forbes, L. V., Magon, N. J., Paton, L. N., Jameson, G. N. L., … Kettle, A. J. (2011). 2-Thioxanthines are mechanism-based inactivators of myeloperoxidase that block oxidative stress during inflammation. Journal of Biological Chemistry, 286(43), 37578-37589. doi: 10.1074/jbc.M111.266981
Briggs, C., Kunka, S., Pennaneach, C., Forbes, L., & Machin, S. J. (2003). Performance evaluation of a new compact hematology analyzer, the Sysmex pocH-100i. Laboratory Hematology, 9(4), 225-233.
Forbes, L. V., Gale, R. E., Pizzey, A., Pouwels, K., Nathwani, A., & Linch, D. C. (2002). An activating mutation in the transmembrane domain of the granulocyte colony-stimulating factor receptor in patients with acute myeloid leukemia. Oncogene, 21(39), 5981-5989. doi: 10.1038/sj.onc.1205767
Forbes, L. V., Truong, O., Wientjes, F. B., Moss, S. J., & Segal, A. W. (1999). The major phosphorylation site of the NADPH oxidase component p67phox is Thr233. Biochemical Journal, 338(1), 99-105. doi: 10.1042/0264-6021:3380099
Reeves, E. P., Dekker, L. V., Forbes, L. V., Wientjes, F. B., Grogan, A., Pappin, D. J. C., & Segal, A. W. (1999). Direct interaction between p47phox and protein kinase C: Evidence for targeting of protein kinase C by p47phox in neutrophils. Biochemical Journal, 344(3), 859-866. doi: 10.1042/0264-6021:3440859
Leino, L., Forbes, L., Segal, A., & Cockcroft, S. (1999). Reconstitution of GTPγS-induced NADPH oxidase activity in streptolysin-O-permeabilized neutrophils by specific cytosol fractions. Biochemical & Biophysical Research Communications, 265(1), 29-37. doi: 10.1006/bbrc.1999.1631
Forbes, L. V., Moss, S. J., & Segal, A. W. (1999). Phosphorylation of p67phox in the neutrophil occurs in the cytosol and is independent of p47phox. FEBS Letters, 449(2-3), 225-229. doi: 10.1016/S0014-5793(99)00444-5
Abbott, W. G. H., Tan, P. L. J., Geursen, A., Fraser, J. D., Forbes, L., Simpson, I. J., … Skinner, M. A. (1998). TCRBV14S1 and rheumatoid arthritis revisited: Abnormalities in the percentage of transcribed TCRBV14S1 family genes in PBMC from rheumatoid arthritis patients. Scandinavian Journal of Rheumatology, 27, 68-75.
Willis, J. A., Scott, R. S., Brown, L. J., Forbes, L. V., Schmidli, R. S., Zimmet, P. Z., … Rowley, M. J. (1996). Islet cell antibodies and antibodies against glutamic acid decarboxylase in newly diagnosed adult-onset diabetes mellitus. Diabetes Research & Clinical Practice, 33(2), 89-97. doi: 10.1016/0168-8227(96)01281-8
Forbes, L. V., Scott, R. S., Brown, L. J., & Darlow, B. A. (1995). Immunogenetic, clinical and demographic characterization of childhood type 1 diabetes in New Zealand. Diabetes Care, 18(11), 1428-1433.
Rudert, F., Visser, E., Forbes, L., Lindridge, E., Wang, L., & Watson, J. (1995). Identification of a silencer, enhancer, and basal promoter region in the human CD95 (Fas/APO-1) gene. DNA & Cell Biology, 14(11), 931-937.
Rudert, F., Roos, M., Forbes, L., & Watson, J. (1994). Apoptosis in L929 cells expressing a CD40/Fas chimeric receptor: Dissociation of stimulatory from inhibitory death signalling functions. Biochemical & Biophysical Research Communications, 204(3), 1102-1110. doi: 10.1006/bbrc.1994.2576
Forbes, L. V., Brown, L. J., & Scott, R. S. (1993). HLA-DQβ typing and non-Asp57 alleles in IDDM and nondiabetic subjects in New Zealand. Diabetes Care, 16(8), 1179-1183.
Schmidli, R. S., Hagan, C., Scott, R. S., Livesey, J., & Forbes, L. V. (1993). Plasma proinsulin in recently diagnosed type 2 diabetes mellitus. Diabetes Research & Clinical Practice, 20(2), 133-138. doi: 10.1016/0168-8227(93)90007-R
Scott, R. S., Brown, L. J., Darlow, B. A., Forbes, L. V., & Moore, M. P. (1992). Temporal variation in incidence of IDDM in Canterbury, New Zealand. Diabetes Care, 15(7), 895-899. doi: 10.2337/diacare.15.7.895
Journal - Research Other
Ashby, L. V., Springer, R., Hampton, M. B., Kettle, A. J., & Winterbourn, C. C. (2020). Evaluating the bactericidal action of hypochlorous acid in culture media [Short communication]. Free Radical Biology & Medicine. Advance online publication. doi: 10.1016/j.freeradbiomed.2020.07.033
Carr, A., Pullar, J., Forbes, L., & Rosengrave, P. (2014). Working mothers are disadvantaged by limited funding for health research. New Zealand Medical Journal, 127(1398). Retrieved from http://www.nzma.org.nz/journal
Conference Contribution - Published proceedings: Abstract
Hsiao, K.-C., Caldwell, I., Woon, S. T., & Ashby, L. (2020). Novel heterozygous dominant activating RAC2 variant in four New Zealand patients with combined immunodeficiency. Journal of Clinical Immunology, 40(Suppl. 1), (pp. S120-S121). doi: 10.1007/s10875-020-00764-z
Albrett, A. M., Ashby, L. V., Dickerhof, N., Hampton, M. B., Winterbourn, C. C., & Kettle, A. J. (2018). Detection of hypochlorous acid in neutrophil phagosomes with the probe R19S: Chlorinated product formation and real-time fluorescence. Proceedings of the New Zealand Microbiological Society (NZMS) New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) Joint Annual Conference: Microbes & Molecules. (pp. 112-113). Retrieved from https://www.nzmsconference.org.nz
Kettle, A., Albrett, A., Forbes, L., Parker, H., Turner, R., Pattemore, P., & Winterbourn, C. (2014). Bacterial killing and oxidant production by neutrophils from patients with cystic fibrosis. Free Radical Biology & Medicine, 76(Suppl. 1), (pp. S163). doi: 10.1016/j.freeradbiomed.2014.10.047
Forbes, L. V., Sjögren, T., Auchère, F., Thong, B., Eriksson, H., Unitt, J. F., & Kettle, A. (2013). Potent reversible inhibition of myeloperoxidase by aromatic hydroxamates. Free Radical Biology & Medicine, 65(Suppl. 2), (pp. S127). doi: 10.1016/j.freeradbiomed.2013.10.712
Conference Contribution - Poster Presentation (not in published proceedings)
Springer, R., Ashby, L., Kettle, A., Winterbourn, C., & Hampton, M. (2018, September). Staphylococcus Aureus survival inside the neutrophil phagosome. Poster session presented at the Otago Spotlight Symposium on Infectious Disease, Wellington, New Zealand.
Forbes, L. V., Parker, H., Turner, R., Albrett, A., & Kettle, A. J. (2014, December). Myeloperoxidase and protein oxidation in the neutrophil phagosome. Poster session presented at the Society for Free Radical Research Australasia 22nd Annual Scientific Meeting, Melbourne, Australia.
Forbes, L. V., & Kettle, A. J. (2010, November-December). Hydrazides as inhibitors of myeloperoxidase. Poster session presented at the Society for Free Radical Research (SFRR) Australasia 19th Annual Conference, Akaroa, New Zealand.
Conference Contribution - Verbal presentation and other Conference outputs
Forbes, L. V., Konigstorfer, A., Gray, M. J., Jakob, U., Kettle, A. J., Hampton, M. B., & Winterbourn, C. C. (2017, July). Bacterial resistance to neutrophil-derived oxidants. Verbal presentation at the New Zealand Branch of the Australasian Society for Immunology (NZ ASI) Annual Scientific Meeting, Christchurch, New Zealand.
Kettle, A., Albrett, A., Forbes, L., Parker, H., Turner, R., Pattemore, P., & Winterbourn, C. (2014, November). Bacterial killing and oxidant production by neutrophils from patients with cystic fibrosis. Verbal presentation at the Society for Free Radical Biology and Medicine (SFRBM) 21st Annual Meeting, Seattle, WA.
Forbes, L. V., Sjögren, T., Auchère, F., Jenkins, D. W., Thong, B., Laughton, D., … Kettle, A. J. (2013, August). Potent reversible inhibition of myeloperoxidase by aromatic hydroxamates. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.
