Contact Details
- Phone
- +64 3 479 3140
- sergio.morales@otago.ac.nz
- Position
- Associate Professor
- Department
- Department of Microbiology and Immunology
- Qualifications
- BSc, PhD
- Research summary
- Environmental microbiology, microbial biotechnology and microbial ecology, bacterial and viral genetics
Research
Our goal is to understand how microbes survive in the environment, and how their presence affects ecosystem processes like primary productivity and nutrient turnover.
By understanding how microorganisms adapt and respond to environmental cues we can harness some of their power, and predict how ecosystems will react to environmental changes.
Publications
Pearman, W. S., Morales, S. E., Vaux, F., Gemmell, N. J., & Fraser, C. I. (2024). Host population crashes disrupt the diversity of associated marine microbiomes. Environmental Microbiology, 26, e16611. doi: 10.1111/1462-2920.16611
Pearman, W. S., Duffy, G. A., Liu, X. P., Gemmell, N. J., Morales, S. E., & Fraser, C. I. (2023). Macroalgal microbiome biogeography is shaped by environmental drivers rather than geographic distance. Annals of Botany. Advance online publication. doi: 10.1093/aob/mcad151
Baltar, F., Martínez-Pérez, C., Amano, C., Vial, M., Robaina-Estévez, S., Reinthaler, T., … Morales, S. E., & González, J. M. (2023). A ubiquitous gammaproteobacterial clade dominates expression of sulfur oxidation genes across the mesopelagic ocean. Nature Microbiology. Advance online publication. doi: 10.1038/s41564-023-01374-2
Lappan, R., Shelley, G., Islam, Z. F., Leung, P. M., Lockwood, S., Nauer, P. A., … Morales, S. E., & Greening, C. (2023). Molecular hydrogen in seawater supports growth of diverse marine bacteria. Nature Microbiology. Advance online publication. doi: 10.1038/s41564-023-01322-0
Highton, M. P., Bakken, L. R., Dörsch, P., Tobias‑Hunefeldt, S., Molstad, L., & Morales, S. E. (2023). Soil water extract and bacteriome determine N2O emission potential in soils. Biology & Fertility of Soils. Advance online publication. doi: 10.1007/s00374-022-01690-5
Morales, S. E., & Holben, W. E. (2011). Empirical testing of 16S PCR primer pairs reveals variance in target specificity and efficacy not suggested by in silico analysis. In F. J. de Bruijn (Ed.), Handbook of molecular microbial ecology I: Metagenomics and complementary approaches. (pp. 135-141). Wiley-Blackwell.
Chapter in Book - Research
Morales, S. E., Cosart, T. F., Johnson, J. V., & Holben, W. E. (2011). Extensive phylogenetic analysis of a soil bacterial community illustrates extreme taxon evenness and the effects of amplicon length, degree of coverage, and DNA fractionation on classification and ecological parameters. In F. J. de Bruijn (Ed.), Handbook of molecular microbial ecology II: Metagenomics in different habitats. (pp. 151-164). Wiley-Blackwell.
Chapter in Book - Research
Pearman, W. S., Morales, S. E., Vaux, F., Gemmell, N. J., & Fraser, C. I. (2024). Host population crashes disrupt the diversity of associated marine microbiomes. Environmental Microbiology, 26, e16611. doi: 10.1111/1462-2920.16611
Journal - Research Article
Baltar, F., Martínez-Pérez, C., Amano, C., Vial, M., Robaina-Estévez, S., Reinthaler, T., … Morales, S. E., & González, J. M. (2023). A ubiquitous gammaproteobacterial clade dominates expression of sulfur oxidation genes across the mesopelagic ocean. Nature Microbiology. Advance online publication. doi: 10.1038/s41564-023-01374-2
Journal - Research Article
Décima, M., Stukel, M. R., Nodder, S. D., Gutiérrez Rodríguez, A., Selph, K. E., dos Santos, A. L., … Deans, F., Morales, S. E., … Pinkerton, M. (2023). Salp blooms drive strong increases in passive carbon export in the Southern Ocean. Nature Communications, 14, 425. doi: 10.1038/s41467-022-35204-6
Journal - Research Article
Highton, M. P., Bakken, L. R., Dörsch, P., Tobias‑Hunefeldt, S., Molstad, L., & Morales, S. E. (2023). Soil water extract and bacteriome determine N2O emission potential in soils. Biology & Fertility of Soils. Advance online publication. doi: 10.1007/s00374-022-01690-5
Journal - Research Article
Lappan, R., Shelley, G., Islam, Z. F., Leung, P. M., Lockwood, S., Nauer, P. A., … Morales, S. E., & Greening, C. (2023). Molecular hydrogen in seawater supports growth of diverse marine bacteria. Nature Microbiology. Advance online publication. doi: 10.1038/s41564-023-01322-0
Journal - Research Article
Pearman, W. S., Duffy, G. A., Liu, X. P., Gemmell, N. J., Morales, S. E., & Fraser, C. I. (2023). Macroalgal microbiome biogeography is shaped by environmental drivers rather than geographic distance. Annals of Botany. Advance online publication. doi: 10.1093/aob/mcad151
Journal - Research Article
Highton, M. P., Bakken, L. R., Dörsch, P., Molstad, L., & Morales, S. E. (2022). Nitrite accumulation and impairment of N2O reduction explains contrasting soil denitrification phenotypes. Soil Biology & Biochemistry, 166, 108529. doi: 10.1016/j.soilbio.2021.108529
Journal - Research Article
Lockwood, S., Greening, C., Baltar, F., & Morales, S. E. (2022). Global and seasonal variation of marine phosphonate metabolism. ISME Journal, 16, 2198-2212. doi: 10.1038/s41396-022-01266-z
Journal - Research Article
Martínez-Pérez, C., Greening, C., Bay, S. K., Lappan, R. J., Zhao, Z., De Corte, D., Hulbe, C., Ohneiser, C., … Thomson, B., … Morales, S. E., & Baltar, F. (2022). Phylogenetically and functionally diverse microorganisms reside under the Ross Ice Shelf. Nature Communications, 13, 117. doi: 10.1038/s41467-021-27769-5
Journal - Research Article
Wang, X., Reilly, K., Heathcott, R., Biswas, A., Johnson, L. J., Teasdale, S., … Morales, S. E. (2022). Soil nitrogen treatment alters microbiome networks across farm niches. Frontiers in Microbiology, 12, 786156. doi: 10.3389/fmicb.2021.786156
Journal - Research Article
Espinel-Velasco, N., Tobias-Hünefeldt, S. P., Karelitz, S. E., Hoffmann, L. J., Morales, S. E., & Lamare, M. D. (2021). Reduced seawater pH alters marine biofilms with impacts for marine polychaete larval settlement. Marine Environmental Research, 167, 105291. doi: 10.1016/j.marenvres.2021.105291
Journal - Research Article
Ganasamurthy, S., Rex, D., Samad, M. S., Richards, K. G., Lanigan, G. J., Grelet, G.-Ä., … Morales, S. E. (2021). Competition and community succession link N transformation and greenhouse gas emissions in urine patches. Science of the Total Environment, 779, 146318. doi: 10.1016/j.scitotenv.2021.146318
Journal - Research Article
Samad, M. S., Ganasamurthy, S., Highton, M. P., Bakken, L. R., Clough, T. J., de Klein, C. A. M., … Morales, S. E. (2021). Urea treatment decouples intrinsic pH control over N2O emissions in soils. Soil Biology & Biochemistry, 163, 108461. doi: 10.1016/j.soilbio.2021.108461
Journal - Research Article
Tobias-Hünefeldt, S. P., Wenley, J., Baltar, F., & Morales, S. E. (2021). Ecological drivers switch from bottom-up to top-down during model microbial community successions. ISME Journal, 15, 1085-1097. doi: 10.1038/s41396-020-00833-6
Journal - Research Article
Tobias-Hünefeldt, S. P., Wing, S. R., Baltar, F., & Morales, S. E. (2021). Changes in microbial community phylogeny and metabolic activity along the water column uncouple at near sediment aphotic layers in fjords. Scientific Reports, 11, 19303. doi: 10.1038/s41598-021-98519-2
Journal - Research Article
van der Weerden, T. J., Rutherford, A. J., de Klein, C. A. M., Ganasamurthy, S., & Morales, S. E. (2021). Elevating soil pH does not reduce N2O emissions from urine deposited onto pastoral soils. New Zealand Journal of Agricultural Research. doi: 10.1080/00288233.2021.1935280
Journal - Research Article
Wenley, J., Currie, K., Lockwood, S., Thomson, B., Baltar, F., & Morales, S. E. (2021). Seasonal prokaryotic community linkages between surface and deep ocean water. Frontiers in Marine Science, 8, 659641. doi: 10.3389/fmars.2021.659641
Journal - Research Article
Bagnaro, A., Baltar, F., Brownstein, G., Lee, W. G., Morales, S. E., Pritchard, D. W., & Hepburn, C. D. (2020). Reducing the arbitrary: Fuzzy detection of microbial ecotones and ecosystems: Focus on the pelagic environment. Environmental Microbiome, 15, 16. doi: 10.1186/s40793-020-00363-w
Journal - Research Article
Highton, M. P., Bakken, L. R., Dörsch, P., Wakelin, S., de Klein, C. A. M., Molstad, L., & Morales, S. E. (2020). Soil N2O emission potential falls along a denitrification phenotype gradient linked to differences in microbiome, rainfall and carbon availability. Soil Biology & Biochemistry, 150, 108004. doi: 10.1016/j.soilbio.2020.108004
Journal - Research Article
Nelson, K. S., Baltar, F., Lamare, M. D., & Morales, S. E. (2020). Ocean acidification affects microbial community and invertebrate settlement on biofilms. Scientific Reports, 10, 3274. doi: 10.1038/s41598-020-60023-4
Journal - Research Article
Gardner, P. P., Watson, R. J., Morgan, X. C., Draper, J. L., Finn, R. D., Morales, S. E., & Stott, M. B. (2019). Identifying accurate metagenome and amplicon software via a meta-analysis of sequence to taxonomy benchmarking studies. PeerJ, 7, e6160 . doi: 10.7717/peerj.6160
Journal - Research Article
Greening, C., Geier, R., Wang, C., Woods, L. C., Morales, S. E., McDonald, M. J., Rushton-Green, R., Morgan, X. C., … Cook, G. M., & Mackie, R. I. (2019). Diverse hydrogen production and consumption pathways influence methane production in ruminants. ISME Journal, 13, 2617-2632. doi: 10.1038/s41396-019-0464-2
Journal - Research Article
Morales, S. E., Biswas, A., Herndl, G. J., & Baltar, F. (2019). Global structuring of phylogenetic and functional diversity of pelagic fungi by depth and temperature. Frontiers in Marine Science, 6, 131. doi: 10.3389/fmars.2019.00131
Journal - Research Article
Rex, D., Clough, T. J., Richards, K. G., Condron, L. M., de Klein, C. A. M., Morales, S. E., & Lanigan, G. J. (2019). Impact of nitrogen compounds on fungal and bacterial contributions to codenitrification in a pasture soil. Scientific Reports, 9, 13371. doi: 10.1038/s41598-019-49989-y
Journal - Research Article
Tobias-Hünefeldt, S. P., Wing, S. R., Espinel-Velasco, N., Baltar, F., & Morales, S. E. (2019). Depth and location influence prokaryotic and eukaryotic microbial community structure in New Zealand fjords. Science of the Total Environment, 693, 133507. doi: 10.1016/j.scitotenv.2019.07.313
Journal - Research Article
Baltar, F., Gutiérrez-Rodriguez, A., Meyer, M., Skudelny, I., Sanders, S., Thomson, B., … Morales, S. E. (2018). Specific effect of trace metals on marine heterotrophic microbial activity and diversity: Key role of iron and zinc and hydrocarbon-degrading bacteria. Frontiers in Microbiology, 9, 3190. doi: 10.3389/fmicb.2018.03190
Journal - Research Article
Kaminsky, R., & Morales, S. E. (2018). Conditionally rare taxa contribute but do not account for changes in soil prokaryotic community structure. Frontiers in Microbiology, 9, 809. doi: 10.3389/fmicb.2018.00809
Journal - Research Article
Lear, G., Dickie, I., Banks, J., Boyer, S., Buckley, H. L., Buckley, T. R., … Morales, S. E., Orlovich, D. A., … Holdaway, R. (2018). Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples. New Zealand Journal of Ecology, 42(1), 10. doi: 10.20417/nzjecol.42.9
Journal - Research Article
Morales, S. E., Meyer, M., Currie, K., & Baltar, F. (2018). Are oceanic fronts ecotones? Seasonal changes along the subtropical front show fronts as bacterioplankton transition zones but not diversity hotspots. Environmental Microbiology Reports, 10(2), 184-189. doi: 10.1111/1758-2229.12618
Journal - Research Article
Morales, S. E., Trouche, B., Wakelin, S., Banabas, M., & Nelson, P. N. (2018). Shifts in prokaryotic communities under forest and grassland within a tropical mosaic landscape. Applied Soil Ecology, 125, 156-161. doi: 10.1016/j.apsoil.2017.11.028
Journal - Research Article
Peura, S., Buck, M., Aalto, S. L., Morales, S. E., Nykänen, H., & Eller, A. (2018). Novel Autotrophic Organisms Contribute Significantly to the Internal Carbon Cycling Potential of a Boreal Lake. mBio, 9(4), 00916-18. doi: 10.1128/mBio.00916-18
Journal - Research Article
Rex, D., Clough, T. J., Richards, K. G., de Klein, C., Morales, S. E., Samad, M. S., … Lanigan, G. J. (2018). Fungal and bacterial contributions to codenitrification emissions of N2O and N2 following urea deposition to soil. Nutrient Cycling in Agroecosystems, 110(1), 135-149. doi: 10.1007/s10705-017-9901-7
Journal - Research Article
Clough, T. J., Lanigan, G. J., de Klein, C. A. M., Samad, M. S., Morales, S. E., Rex, D., … Richards, K. G. (2017). Influence of soil moisture on codenitrification fluxes from a urea-affected pasture soil. Scientific Reports, 7, 2185. doi: 10.1038/s41598-017-02278-y
Journal - Research Article
Kaminsky, R., Trouche, B., & Morales, S. E. (2017). Soil classification predicts differences in prokaryotic communities across a range of geographically distant soils once pH is accounted for. Scientific Reports, 7, 45369. doi: 10.1038/srep45369
Journal - Research Article
Ney, B., Ahmed, F. H., Carere, C. R., Biswas, A., Warden, A. C., Morales, S. E., … Greening, C. (2017). The methanogenic redox cofactor F420 is widely synthesized by aerobic soil bacteria. ISME Journal, 11, 125-137. doi: 10.1038/ismej.2016.100
Journal - Research Article
Samad, M. S., Johns, C., Richards, K. G., Lanigan, G. J., de Klein, C. A. M., Clough, T. J., & Morales, S. E. (2017). Response to nitrogen addition reveals metabolic and ecological strategies of soil bacteria. Molecular Ecology, 26(20), 5500-5514. doi: 10.1111/mec.14275
Journal - Research Article
Baltar, F., Currie, K., Stuck, E., Roosa, S., & Morales, S. E. (2016). Oceanic fronts: Transition zones for bacterioplankton community composition. Environmental Microbiology Reports, 8(1), 132-138. doi: 10.1111/1758-2229.12362
Journal - Research Article
Biswas, A., Staals, R. H. J., Morales, S. E., Fineran, P. C., & Brown, C. M. (2016). CRISPRDetect: A flexible algorithm to define CRISPR arrays. BMC Genomics, 17, 356. doi: 10.1186/s12864-016-2627-0
Journal - Research Article
Greening, C., Biswas, A., Carere, C. R., Jackson, C. J., Taylor, M. C., Stott, M. B., Cook, G. M., & Morales, S. E. (2016). Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival. ISME Journal, 10, 761-777. doi: 10.1038/ismej.2015.153
Journal - Research Article
Highton, M. P., Roosa, S., Crawshaw, J., Schallenberg, M., & Morales, S. E. (2016). Physical factors correlate to microbial community structure and nitrogen cycling gene abundance in a nitrate fed eutrophic lagoon. Frontiers in Microbiology, 7, 1691. doi: 10.3389/fmicb.2016.01691
Journal - Research Article
Samad, M. S., Bakken, L. R., Nadeem, S., Clough, T. J., de Klein, C. A. M., Richards, K. G., … Morales, S. E. (2016). High-resolution denitrification kinetics in pasture soils link N2O emissions to pH, and denitrification to C mineralization. PLoS ONE, 11(3), e0151713. doi: 10.1371/journal.pone.0151713
Journal - Research Article
Samad, M. S., Biswas, A., Bakken, L. R., Clough, T. J., de Klein, C. A. M., Richards, K. G., … Morales, S. E. (2016). Phylogenetic and functional potential links pH and N2O emissions in pasture soils. Scientific Reports, 6, 35990. doi: 10.1038/srep35990
Journal - Research Article
Wolf, P. G., Biswas, A., Morales, S. E., Greening, C., & Gaskins, H. R. (2016). H2 metabolism is widespread and diverse among human colonic microbes. Gut Microbes, 7(3), 235-245. doi: 10.1080/19490976.2016.1182288
Journal - Research Article
Baltar, F., Stuck, E., Morales, S., & Currie, K. (2015). Bacterioplankton carbon cycling along the Subtropical Frontal Zone off New Zealand. Progress in Oceanography, 135, 168-175. doi: 10.1016/j.pocean.2015.05.019
Journal - Research Article
Greening, C., Carere, C. R., Rushton-Green, R., Harold, L. K., Hards, K., Taylor, M. C., Morales, S. E., … Cook, G. M. (2015). Persistence of the dominant soil phylum Acidobacteria by trace gas scavenging. PNAS, 112(33), 10497-10502. doi: 10.1073/pnas.1508385112
Journal - Research Article
Greening, C., Constant, P., Hards, K., Morales, S. E., Oakeshott, J. G., Russell, R. J., … Cook, G. M. (2015). Atmospheric hydrogen scavenging: From enzymes to ecosystems. Applied & Environmental Microbiology, 81(4), 1190-1199. doi: 10.1128/aem.03364-14
Journal - Research Article
Morales, S. E., Jha, N., & Saggar, S. (2015). Biogeography and biophysicochemical traits link N2O emissions, N2O emission potential and microbial communities across New Zealand pasture soils. Soil Biology & Biochemistry, 82, 87-98. doi: 10.1016/j.soilbio.2014.12.018
Journal - Research Article
Morales, S. E., Jha, N., & Saggar, S. (2015). Impact of urine and the application of the nitrification inhibitor DCD on microbial communities in dairy-grazed pasture soils. Soil Biology & Biochemistry, 88(0), 344-353. doi: 10.1016/j.soilbio.2015.06.009
Journal - Research Article
Morales, S. E., & Holben, W. E. (2014). Simulated geologic carbon storage leak reduces bacterial richness and alters bacterial community composition in surface soil. Soil Biology & Biochemistry, 76, 286-296. doi: 10.1016/j.soilbio.2014.05.018
Journal - Research Article
Morales, S. E., & Holben, W. E. (2013). Functional response of a near-surface soil microbial community to a simulated underground CO2 storage leak. PLoS ONE, 8(11), e81742. doi: 10.1371/journal.pone.0081742
Journal - Research Article
Gibbons, S. M., Feris, K., McGuirl, M. A., Morales, S. E., Hynninen, A., Ramsey, P. W., & Gannon, J. E. (2011). Use of microcalorimetry to determine the costs and benefits to Pseudomonas putida strain KT2440 of harboring cadmium efflux genes. Applied & Environmental Microbiology, 77(1), 108-113. doi: 10.1128/AEM.01187-10
Journal - Research Article
Morales, S. E., Cosart, T., & Holben, W. E. (2010). Bacterial gene abundances as indicators of greenhouse gas emission in soils. ISME Journal, 4, 799-808. doi: 10.1038/ismej.2010.8
Journal - Research Article
Mouser, P. J., Rizzo, D. M., Druschel, G. K., Morales, S. E., Hayden, N., O'Grady, P., & Stevens, L. (2010). Enhanced detection of groundwater contamination from a leaking waste disposal site by microbial community profiles. Water Resources Research, 46, W12506. doi: 10.1029/2010WR009459
Journal - Research Article
Morales, S. E., & Holben, W. E. (2009). Empirical testing of 16S rRNA gene PCR primer pairs reveals variance in target specificity and efficacy not suggested by in silico analysis. Applied & Environmental Microbiology, 75(9), 2677-2683. doi: 10.1128/AEM.02166-08
Journal - Research Article
Morales, S. E., Cosart, T. F., Johnson, J. V., & Holben, W. E. (2009). Extensive phylogenetic analysis of a soil bacterial community illustrates extreme taxon evenness and the effects of amplicon length, degree of coverage, and DNA fractionation on classification and ecological parameters. Applied & Environmental Microbiology, 75(3), 668-675. doi: 10.1128/AEM.01757-08
Journal - Research Article
Gotelli, N. J., Mouser, P. J., Hudman, S. P., Morales, S. E., Ross, D. S., & Ellison, A. M. (2008). Geographic variation in nutrient avalibility, stoichiometry, and metal concentrations of plants and pore-water in ombrotrophic bogs in New England, USA. Wetlands, 28(3), 827-840. doi: 10.1672/07-165.1
Journal - Research Article
Morales, S. E., Cosart, T., Johnson, J. V., & Holben, W. E. (2008). Supplemental programs for enhanced recovery of data from the DOTUR application. Journal of Microbiological Methods, 75(3), 572-575. doi: 10.1016/j.mimet.2008.07.017
Journal - Research Article
Morales, S. E., & Lewis, T. A. (2006). Transcriptional regulation of the pdt gene cluster of Pseudomonas stutzeri KC involves an AraC/XylS family transcriptional activator (PdtC) and the cognate siderophore pyridine-2,6-Bis(thiocarboxylic acid). Applied & Environmental Microbiology, 72(11), 6994-7002. doi: 10.1128/AEM.01518-06
Journal - Research Article
Morales, S. E., Mouser, P. J., Ward, N., Hudman, S. P., Gotelli, N. J., Ross, D. S., & Lewis, T. A. (2006). Comparison of bacterial communities in New England Sphagnum bogs using terminal restriction fragment length polymorphism (T-RFLP). Microbial Ecology, 52(1), 34-44. doi: 10.1007/s00248-005-0264-2
Journal - Research Article
Meaden, S., Biswas, A., Arkhipova, K., Morales, S. E., Dutilh, B. E., . . ., & Fineran, P. C. (2022). High viral abundance and low diversity are associated with increased CRISPR-Cas prevalence across microbial ecosystems. Current Biology, 32, 220-227. doi: 10.1016/j.cub.2021.10.038
Journal - Research Other
Morales, S. E., Hulbe, C., Martínez-Pérez, C., & Baltar, F. (2022). In the dark, freezing ocean under Antarctica's largest ice shelf, we discovered a thriving microbial jungle. The Conversation. Retrieved from https://theconversation.com/in-the-dark-freezing-ocean-under-antarcticas-largest-ice-shelf-we-discovered-a-thriving-microbial-jungle-175735
Journal - Research Other
Hassanzadeh, B., Thomson, B., Deans, F., Wenley, J., Lockwood, S., Currie, K., Morales, S. E., … Gómez-Consarnau, L. (2021). Microbial rhodopsins are increasingly favoured over chlorophyll in High Nutrient Low Chlorophyll waters. Environmental Microbiology Reports, 13(3), 401-406. doi: 10.1111/1758-2229.12948
Journal - Research Other
Kaminsky, R. A., Wakelin, S. A., Highton, M. P., Samad, M. S., & Morales, S. E. (2021). Resolving broad patterns of prokaryotic community structure in New Zealand pasture soils. New Zealand Journal of Agricultural Research, 64(2), 143-161. doi: 10.1080/00288233.2019.1678491
Journal - Research Other
Dickie, I. A., Boyer, S., Buckley, H., Duncan, R. P., Gardner, P., Hogg, I. D., … Morales, S. E., … Weaver, L. (2018). Towards robust and repeatable sampling methods in eDNA based studies. Molecular Ecology Resources, 18(5), 940-952. doi: 10.1111/1755-0998.12907
Journal - Research Other
Morales, S. E., & Holben, W. E. (2011). Linking bacterial identities and ecosystem processes: Can 'omic' analyses be more than the sum of their parts? FEMS Microbiology Ecology, 75(1), 2-16. doi: 10.1111/j.1574-6941.2010.00938.x
Journal - Research Other