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Health Sciences profile

Dr Mihnea Bostina

PositionAcademic Director, Otago Centre for Electron Microscopy
DepartmentDepartment of Microbiology and Immunology
QualificationsMSc PhD
Research summaryStructural biology, viral pathogenesis and molecular virology

Research

Our research interests are in understanding molecular architectures and relating those structures to their functional mechanisms. To do this, we are employing cryo-electron microscopy and electron tomography combined with image analysis techniques.

Publications

Jayawardena, N., Burga, L. N., Easingwood, R. A., Takizawa, K., Wolf, M., & Bostina, M. (2018). Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus. PNAS. Advance online publication. doi: 10.1073/pnas.1810664115

Strauss, M., Jayawardena, N., Sun, E., Easingwood, R. A., Burga, L. N., & Bostina, M. (2018). Cryo-electron microscopy structure of Seneca Valley Virus procapsid. Journal of Virology, 92(6), e01927-17. doi: 10.1128/jvi.01927-17

Miles, L. A., Burga, L. N., Gardner, E. E., Bostina, M., Poirier, J. T., & Rudin, C. M. (2017). Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. Journal of Clinical Investigation, 127(8), 2957-2967. doi: 10.1172/jci93472

Basu, K., Lajoie, D., Aumentado-Armstrong, T., Chen, J., Koning, R. I., Bossy, B., Bostina, M., … Rouiller, I. (2017). Molecular mechanism of DRP1 assembly studied in vitro by cryo-electron microscopy. PLoS ONE, 12(6), e0179397. doi: 10.1371/journal.pone.0179397

Fagerlund, R. D., Wilkinson, M. E., Klykov, O., Barendregt, A., Pearce, F. G., Kieper, S. N., Maxwell, H. W. R., … Krause, K. L., Bostina, M., … Staals, R. H. J., & Fineran, P. C. (2017). Spacer capture and integration by a type I-F Cas1–Cas2-3 CRISPR adaptation complex. PNAS, 114(26), E5122-E5128. doi: 10.1073/pnas.1618421114

Chapter in Book - Research

Levy, H., Bostina, M., Filman, D. J., & Hogle, J. M. (2010). Cell entry: A biochemical and structural perspective. In E. Ehrenfeld, E. Domingo & R. P. Roos (Eds.), The Picornaviruses. (pp. 87-104). Washington, DC: ASM Press.

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Journal - Research Article

Jayawardena, N., Burga, L. N., Easingwood, R. A., Takizawa, K., Wolf, M., & Bostina, M. (2018). Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus. PNAS. Advance online publication. doi: 10.1073/pnas.1810664115

Strauss, M., Jayawardena, N., Sun, E., Easingwood, R. A., Burga, L. N., & Bostina, M. (2018). Cryo-electron microscopy structure of Seneca Valley Virus procapsid. Journal of Virology, 92(6), e01927-17. doi: 10.1128/jvi.01927-17

Miles, L. A., Burga, L. N., Gardner, E. E., Bostina, M., Poirier, J. T., & Rudin, C. M. (2017). Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. Journal of Clinical Investigation, 127(8), 2957-2967. doi: 10.1172/jci93472

Fagerlund, R. D., Wilkinson, M. E., Klykov, O., Barendregt, A., Pearce, F. G., Kieper, S. N., Maxwell, H. W. R., … Krause, K. L., Bostina, M., … Staals, R. H. J., & Fineran, P. C. (2017). Spacer capture and integration by a type I-F Cas1–Cas2-3 CRISPR adaptation complex. PNAS, 114(26), E5122-E5128. doi: 10.1073/pnas.1618421114

Basu, K., Lajoie, D., Aumentado-Armstrong, T., Chen, J., Koning, R. I., Bossy, B., Bostina, M., … Rouiller, I. (2017). Molecular mechanism of DRP1 assembly studied in vitro by cryo-electron microscopy. PLoS ONE, 12(6), e0179397. doi: 10.1371/journal.pone.0179397

Hampton, H. G., McNeil, M. B., Paterson, T. J., Ney, B., Williamson, N. R., Easingwood, R. A., Bostina, M., … Fineran, P. C. (2016). CRISPR-Cas gene-editing reveals RsmA and RsmC act through FlhDC to repress the SdhE flavinylation factor and control motility and prodigiosin production in Serratia. Microbiology, 162(6), 1047-1058. doi: 10.1099/mic.0.000283

Frampton, R. A., Lopez Acedo, E., Young, V. L., Chen, D., Tong, B., Taylor, C., Easingwood, R. A., … Kleffmann, T., Bostina, M., & Fineran, P. C. (2015). Genome, proteome and structure of a T7-like bacteriophage of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae. Viruses, 7(7), 3361-3379. doi: 10.3390/v7072776

Schoderboeck, L., Riad, S., Bokor, A. M., Wicky, H. E., Strauss, M., Bostina, M., Oswald, M. J., Empson, R. M., & Hughes, S. M. (2015). Chimeric rabies SADB19-VSVg-pseudotyped lentiviral vectors mediate long-range retrograde transduction from the mouse spinal cord. Gene Therapy, 22, 357-364. doi: 10.1038/gt.2015.3

Kalynych, S., Cherney, M., Bostina, M., Rouiller, I., & Cygler, M. (2015). Quaternary structure of WzzB and WzzE polysaccharide co-polymerases. Protein Science, 24(1), 58-69. doi: 10.1002/pro.2586

Schotte, L., Strauss, M., Thys, B., Halewyck, H., Filman, D. J., Bostina, M., … Rombaut, B. (2014). Mechanism of action and capsid-stabilizing properties of VHHs with an in vitro antipolioviral activity. Journal of Virology, 88(8), 4403-4413. doi: 10.1128/JVI.03402-13

Sverzhinsky, A., Fabre, L., Cottreau, A. L., Biot-Pelletier, D. M. P., Khalil, S., Bostina, M., … Coulton, J. W. (2014). Coordinated rearrangements between cytoplasmic and periplasmic domains of the membrane protein complex ExbB-ExbD of Escherichia coli. Structure, 22(5), 791-797. doi: 10.1016/j.str.2014.02.010

Strauss, M., Levy, H. C., Bostina, M., Filman, D. J., & Hogle, J. M. (2013). RNA transfer from poliovirus 135S particles across membranes is mediated by long umbilical connectors. Journal of Virology, 87(7), 3903-3914. doi: 10.1128/jvi.03209-12

Grishin, A. M., Ajamian, E., Tao, L., Bostina, M., Zhang, L., Trempe, J.-F., … Cygler, M. (2013). Family of phenylacetyl-CoA monooxygenases differs in subunit organization from other monooxygenases. Journal of Structural Biology, 184(2), 147-154. doi: 10.1016/j.jsb.2013.09.012

Abedi, S., Karimi, B., Kazemi, F., Bostina, M., & Vali, H. (2013). Amorphous TiO2 coated into periodic mesoporous organosilicate channels as a new binary photocatalyst for regeneration of carbonyl compounds from oximes under sunlight irradiation. Organic & Biomolecular Chemistry, 11(3), 416-419. doi: 10.1039/c2ob26907d

Kulczyk, A. W., Akabayov, B., Lee, S.-J., Bostina, M., Berkowitz, S. A., & Richardson, C. C. (2012). An interaction between DNA polymerase and helicase is essential for the high processivity of the bacteriophage T7 replisome. Journal of Biological Chemistry, 287(46), 39050-39060. doi: 10.1074/jbc.M112.410647

Karimi, B., Behzadnia, H., Bostina, M., & Vali, H. (2012). A nano-fibrillated mesoporous carbon as an effective support for palladium nanoparticles in the aerobic oxidation of alcohols "on pure water". Chemistry: A European Journal, 18(28), 8634-8640. doi: 10.1002/chem.201200373

Grishin, A. M., Ajamian, E., Zhang, L., Rouiller, I., Bostina, M., & Cygler, M. (2012). Protein-protein interactions in the β-oxidation part of the phenylacetate utilization pathway: Crystal structure of the PaaF-PaaG hydratase-isomerase complex. Journal of Biological Chemistry, 287(45), 37986-37996. doi: 10.1074/jbc.M112.388231

Bostina, M., Levy, H., Filman, D. J., & Hogle, J. M. (2011). Poliovirus RNA is released from the capsid near a twofold symmetry axis. Journal of Virology, 85(2), 776-783. doi: 10.1128/jvi.00531-10

Gopalakrishnan, G., Yam, P. T., Madwar, C., Bostina, M., Rouiller, I., Colman, D. R., & Lennox, R. B. (2011). Label-free visualization of ultrastructural features of artificial synapses via cryo-EM. ACS Chemical Neuroscience, 2(12), 700-704. doi: 10.1021/cn200094j

Levy, H. C., Bostina, M., Filman, D. J., & Hogle, J. M. (2010). Catching a virus in the act of RNA release: A novel poliovirus uncoating intermediate characterized by cryo-electron microscopy. Journal of Virology, 84(9), 4426-4441. doi: 10.1128/jvi.02393-09

Burga, L. N., Tung, N. M., Troyan, S. L., Bostina, M., Konstantinopoulos, P. A., Fountzilas, H., … Wulf, G. M. (2009). Altered proliferation and differentiation properties of primary mammary epithelial cells from BRCA1 mutation carriers. Cancer Research, 69(4), 1273-1278. doi: 10.1158/0008-5472.can-08-2954

Bostina, M., Bubeck, D., Schwartz, C., Nicastro, D., Filman, D. J., & Hogle, J. M. (2007). Single particle cryoelectron tomography characterization of the structure and structural variability of poliovirus-receptor-membrane complex at 30 Å resolution. Journal of Structural Biology, 160(2), 200-210. doi: 10.1016/j.jsb.2007.08.009

Peng, G., Bostina, M., Radermacher, M., Rais, I., Karas, M., & Michel, H. (2006). Biochemical and electron microscopic characterization of the F1F0 ATP synthase from the hyperthermophilic eubacterium Aquifex aeolicus. FEBS Letters, 580(25), 5934-5940. doi: 10.1016/j.febslet.2006.09.062

Bostina, M., Mohsin, B., Kühlbrandt, W., & Collinson, I. (2005). Atomic model of the E. coli membrane-bound protein translocation complex SecYEG. Journal of Molecular Biology, 352(5), 1035-1043. doi: 10.1016/j.jmb.2005.08.005

Raunser, S., Hasse, W., Bostina, M., Parcej, D. N., & Kühlbrandt, W. (2005). High-yield expression, reconstitution and structure of the recombinant, fully functional glutamate transporter GLT-1 from Rattus norvegicus. Journal of Molecular Biology, 351(3), 598-613. doi: 10.1016/j.jmb.2005.06.036

Abdrakhmanova, A., Zickermann, V., Bostina, M., Radermacher, M., Schägger, H., Kerscher, S., & Brandt, U. (2004). Subunit composition of mitochondrial complex I from the yeast Yarrowia lipolytica. Biochimica et Biophysica Acta: Bioenergetics, 1658(1-2), 148-156. doi: 10.1016/j.bbabio.2004.04.019

Peng, G., Fritzsch, G., Zickermann, V., Schägger, H., Mentele, R., Lottspeich, F., Bostina, M., … Michel, H. (2003). Isolation, characterization and electron microscopic single particle analysis of the NADH:Ubiquinone oxidoreductase (complex I) from the hyperthermophilic eubacterium Aquifex aeolicus. Biochemistry, 42(10), 3032-3039. doi: 10.1021/bi026876v

Zickermann, V., Bostina, M., Hunte, C., Ruiz, T., Radermacher, M., & Brandt, U. (2003). Functional implications from an unexpected position of the 49-kDa subunit of NADH:Ubiquinone oxidoreductase. Journal of Biological Chemistry, 278(31), 29072-29078. doi: 10.1074/jbc.M302713200

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