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Health Sciences profile

Associate Professor Mihnea Bostina

PositionAssociate Professor and Academic Director, Otago Centre for Electron Microscopy
DepartmentDepartment of Microbiology and Immunology
QualificationsMSc PhD
Research summaryStructural biology, viral pathogenesis and molecular virology

Research

Our research interests are in understanding molecular architectures and relating those structures to their functional mechanisms. To do this, we employ cryo-electron microscopy and electron tomography combined with image analysis techniques.

Publications

Jayawardena, N., McCarthy, C., Wang, I., Waqqar, S., Burga, L. N., Strauss, M., & Bostina, M. (2022). Characterisation of a Seneca Valley virus thermostable mutant. Virology. Advance online publication. doi: 10.1016/j.virol.2022.08.015

Warring, S. L., Malone, L. M., Jayaraman, J., Easingwood, R. A., Rigano, L. A., Frampton, R. A., … Pitman, A. R., Bostina, M., & Fineran, P. C. (2022). A lipopolysaccharide-dependent phage infects a pseudomonad phytopathogen and can evolve to evade phage resistance. Environmental Microbiology. Advance online publication. doi: 10.1111/1462-2920.16106

Harfoot, R., Lawley, B., Hernández, L. C., Kuang, J., Grant, J., Treece, J. M., LeQueux, S., Day, R., Jack, S., Stanton, J.-A. L., Bostina, M., Ussher, J. E., & Quiñones‑Mateu, M. E. (2022). Characterization of the first SARS-CoV-2 isolates from Aotearoa New Zealand as part of a rapid response to the COVID-19 pandemic. Viruses, 14, 366. doi: 10.3390/v14020366

Wang, I., Gupta, S. K., Ems, G., Jayawardena, N., Strauss, M., & Bostina, M. (2022). Cryo-EM structure of a possum enterovirus. Viruses, 14, 318. doi: 10.3390/v14020318

Velamoor, S., Mitchell, A., Bostina, M., & Harland, D. (2021). Processing hair follicles for transmission electron microscopy. Experimental Dermatology. Advance online publication. doi: 10.1111/exd.14439

Burga, L., & Bostina, M. (2021). Oncolytic Viruses. In D. H. Bamford & M. Zuckerman (Eds.), Encyclopedia of virology (Vol.1). (4th ed.) (pp. 658-661). Amsterdam, The Netherlands: Academic Press. doi: 10.1016/B978-0-12-814515-9.00064-3

Chapter in Book - Research

Levy, H., Bostina, M., Filman, D. J., & Hogle, J. M. (2010). Cell entry: A biochemical and structural perspective. In E. Ehrenfeld, E. Domingo & R. P. Roos (Eds.), The Picornaviruses. (pp. 87-104). Washington, DC: ASM Press.

Chapter in Book - Research

Harfoot, R., Lawley, B., Hernández, L. C., Kuang, J., Grant, J., Treece, J. M., LeQueux, S., Day, R., Jack, S., Stanton, J.-A. L., Bostina, M., Ussher, J. E., & Quiñones‑Mateu, M. E. (2022). Characterization of the first SARS-CoV-2 isolates from Aotearoa New Zealand as part of a rapid response to the COVID-19 pandemic. Viruses, 14, 366. doi: 10.3390/v14020366

Journal - Research Article

Jayawardena, N., McCarthy, C., Wang, I., Waqqar, S., Burga, L. N., Strauss, M., & Bostina, M. (2022). Characterisation of a Seneca Valley virus thermostable mutant. Virology. Advance online publication. doi: 10.1016/j.virol.2022.08.015

Journal - Research Article

Wang, I., Gupta, S. K., Ems, G., Jayawardena, N., Strauss, M., & Bostina, M. (2022). Cryo-EM structure of a possum enterovirus. Viruses, 14, 318. doi: 10.3390/v14020318

Journal - Research Article

Warring, S. L., Malone, L. M., Jayaraman, J., Easingwood, R. A., Rigano, L. A., Frampton, R. A., … Pitman, A. R., Bostina, M., & Fineran, P. C. (2022). A lipopolysaccharide-dependent phage infects a pseudomonad phytopathogen and can evolve to evade phage resistance. Environmental Microbiology. Advance online publication. doi: 10.1111/1462-2920.16106

Journal - Research Article

Jayawardena, N., Miles, L. A., Burga, L. N., Rudin, C., Wolf, M., Poirier, J. T., & Bostina, M. (2021). N-linked glycosylation on anthrax toxin receptor 1 is essential for Seneca Valley virus infection. Viruses, 13(5), 769. doi: 10.3390/v13050769

Journal - Research Article

Velamoor, S., Mitchell, A., Humbel, B. M., Kim, W., Pushparajan, C., Visnovsky, G., Burga, L. N., & Bostina, M. (2020). Visualizing nudivirus assembly and egress. mBio, 11(4), e01333-20. doi: 10.1128/mBio.01333-20

Journal - Research Article

Velamoor, S., Richena, M., Mitchell, A., Lequeux, S., Bostina, M., & Harland, D. (2020). High-pressure freezing followed by freeze substitution of a complex and variable density miniorgan: The wool follicle. Journal of Microscopy, 278(1), 18-28. doi: 10.1111/jmi.12875

Journal - Research Article

Harland, D. P., Novotná, V., Richena, M., Bostina, M., Velamoor, S., & McKinnon, A. J. (2019). Hair-structure mystery solved by datamining two decades of electron tomograms. Microscopy & Microanalysis, 25(Suppl. 2), 1348-1349. doi: 10.1017/S1431927619007475

Journal - Research Article

Harland, D. P., Novotna, V., Richena, M., Velamoor, S., Bostina, M., & McKinnon, A. J. (2019). Helical twist direction in the macrofibrils of keratin fibres is left handed. Journal of Structural Biology, 206, 345-348. doi: 10.1016/j.jsb.2019.04.007

Journal - Research Article

Kim, S. H., Mercer, A., Mitchell, A., de Miranda, J. R., Ward, V., Mondet, F., & Bostina, M. (2019). Viral infections alter antennal epithelium ultrastructure in honey bees. Journal of Invertebrate Pathology, 168, 107252. doi: 10.1016/j.jip.2019.107252

Journal - Research Article

McCarthy, C., Jayawardena, N., Burga, L. N., & Bostina, M. (2019). Developing picornaviruses for cancer therapy. Cancers, 11(5), 685. doi: 10.3390/cancers11050685

Journal - Research Article

Watson, B. N. J., Easingwood, R. A., Tong, B., Wolf, M., Salmond, G. P. C., Staals, R. H. J., Bostina, M., & Fineran, P. C. (2019). Different genetic and morphological outcomes for phages targeted by single or multiple CRISPR-Cas spacers. Philosophical Transactions of the Royal Society B, 374(1772), 20180090. doi: 10.1098/rstb.2018.0090

Journal - Research Article

Jayawardena, N., Burga, L. N., Easingwood, R. A., Takizawa, K., Wolf, M., & Bostina, M. (2018). Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus. PNAS, 115(46), E10934-E10940. doi: 10.1073/pnas.1810664115

Journal - Research Article

Strauss, M., Jayawardena, N., Sun, E., Easingwood, R. A., Burga, L. N., & Bostina, M. (2018). Cryo-electron microscopy structure of Seneca Valley Virus procapsid. Journal of Virology, 92(6), e01927-17. doi: 10.1128/jvi.01927-17

Journal - Research Article

Basu, K., Lajoie, D., Aumentado-Armstrong, T., Chen, J., Koning, R. I., Bossy, B., Bostina, M., … Rouiller, I. (2017). Molecular mechanism of DRP1 assembly studied in vitro by cryo-electron microscopy. PLoS ONE, 12(6), e0179397. doi: 10.1371/journal.pone.0179397

Journal - Research Article

Fagerlund, R. D., Wilkinson, M. E., Klykov, O., Barendregt, A., Pearce, F. G., Kieper, S. N., Maxwell, H. W. R., … Krause, K. L., Bostina, M., … Staals, R. H. J., & Fineran, P. C. (2017). Spacer capture and integration by a type I-F Cas1–Cas2-3 CRISPR adaptation complex. PNAS, 114(26), E5122-E5128. doi: 10.1073/pnas.1618421114

Journal - Research Article

Miles, L. A., Burga, L. N., Gardner, E. E., Bostina, M., Poirier, J. T., & Rudin, C. M. (2017). Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. Journal of Clinical Investigation, 127(8), 2957-2967. doi: 10.1172/jci93472

Journal - Research Article

Hampton, H. G., McNeil, M. B., Paterson, T. J., Ney, B., Williamson, N. R., Easingwood, R. A., Bostina, M., … Fineran, P. C. (2016). CRISPR-Cas gene-editing reveals RsmA and RsmC act through FlhDC to repress the SdhE flavinylation factor and control motility and prodigiosin production in Serratia. Microbiology, 162(6), 1047-1058. doi: 10.1099/mic.0.000283

Journal - Research Article

Frampton, R. A., Lopez Acedo, E., Young, V. L., Chen, D., Tong, B., Taylor, C., Easingwood, R. A., … Kleffmann, T., Bostina, M., & Fineran, P. C. (2015). Genome, proteome and structure of a T7-like bacteriophage of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae. Viruses, 7(7), 3361-3379. doi: 10.3390/v7072776

Journal - Research Article

Kalynych, S., Cherney, M., Bostina, M., Rouiller, I., & Cygler, M. (2015). Quaternary structure of WzzB and WzzE polysaccharide co-polymerases. Protein Science, 24(1), 58-69. doi: 10.1002/pro.2586

Journal - Research Article

Schoderboeck, L., Riad, S., Bokor, A. M., Wicky, H. E., Strauss, M., Bostina, M., Oswald, M. J., Empson, R. M., & Hughes, S. M. (2015). Chimeric rabies SADB19-VSVg-pseudotyped lentiviral vectors mediate long-range retrograde transduction from the mouse spinal cord. Gene Therapy, 22, 357-364. doi: 10.1038/gt.2015.3

Journal - Research Article

Schotte, L., Strauss, M., Thys, B., Halewyck, H., Filman, D. J., Bostina, M., … Rombaut, B. (2014). Mechanism of action and capsid-stabilizing properties of VHHs with an in vitro antipolioviral activity. Journal of Virology, 88(8), 4403-4413. doi: 10.1128/JVI.03402-13

Journal - Research Article

Sverzhinsky, A., Fabre, L., Cottreau, A. L., Biot-Pelletier, D. M. P., Khalil, S., Bostina, M., … Coulton, J. W. (2014). Coordinated rearrangements between cytoplasmic and periplasmic domains of the membrane protein complex ExbB-ExbD of Escherichia coli. Structure, 22(5), 791-797. doi: 10.1016/j.str.2014.02.010

Journal - Research Article

Abedi, S., Karimi, B., Kazemi, F., Bostina, M., & Vali, H. (2013). Amorphous TiO2 coated into periodic mesoporous organosilicate channels as a new binary photocatalyst for regeneration of carbonyl compounds from oximes under sunlight irradiation. Organic & Biomolecular Chemistry, 11(3), 416-419. doi: 10.1039/c2ob26907d

Journal - Research Article

Grishin, A. M., Ajamian, E., Tao, L., Bostina, M., Zhang, L., Trempe, J.-F., … Cygler, M. (2013). Family of phenylacetyl-CoA monooxygenases differs in subunit organization from other monooxygenases. Journal of Structural Biology, 184(2), 147-154. doi: 10.1016/j.jsb.2013.09.012

Journal - Research Article

Strauss, M., Levy, H. C., Bostina, M., Filman, D. J., & Hogle, J. M. (2013). RNA transfer from poliovirus 135S particles across membranes is mediated by long umbilical connectors. Journal of Virology, 87(7), 3903-3914. doi: 10.1128/jvi.03209-12

Journal - Research Article

Grishin, A. M., Ajamian, E., Zhang, L., Rouiller, I., Bostina, M., & Cygler, M. (2012). Protein-protein interactions in the β-oxidation part of the phenylacetate utilization pathway: Crystal structure of the PaaF-PaaG hydratase-isomerase complex. Journal of Biological Chemistry, 287(45), 37986-37996. doi: 10.1074/jbc.M112.388231

Journal - Research Article

Karimi, B., Behzadnia, H., Bostina, M., & Vali, H. (2012). A nano-fibrillated mesoporous carbon as an effective support for palladium nanoparticles in the aerobic oxidation of alcohols "on pure water". Chemistry: A European Journal, 18(28), 8634-8640. doi: 10.1002/chem.201200373

Journal - Research Article

Kulczyk, A. W., Akabayov, B., Lee, S.-J., Bostina, M., Berkowitz, S. A., & Richardson, C. C. (2012). An interaction between DNA polymerase and helicase is essential for the high processivity of the bacteriophage T7 replisome. Journal of Biological Chemistry, 287(46), 39050-39060. doi: 10.1074/jbc.M112.410647

Journal - Research Article

Bostina, M., Levy, H., Filman, D. J., & Hogle, J. M. (2011). Poliovirus RNA is released from the capsid near a twofold symmetry axis. Journal of Virology, 85(2), 776-783. doi: 10.1128/jvi.00531-10

Journal - Research Article

Gopalakrishnan, G., Yam, P. T., Madwar, C., Bostina, M., Rouiller, I., Colman, D. R., & Lennox, R. B. (2011). Label-free visualization of ultrastructural features of artificial synapses via cryo-EM. ACS Chemical Neuroscience, 2(12), 700-704. doi: 10.1021/cn200094j

Journal - Research Article

Levy, H. C., Bostina, M., Filman, D. J., & Hogle, J. M. (2010). Catching a virus in the act of RNA release: A novel poliovirus uncoating intermediate characterized by cryo-electron microscopy. Journal of Virology, 84(9), 4426-4441. doi: 10.1128/jvi.02393-09

Journal - Research Article

Burga, L. N., Tung, N. M., Troyan, S. L., Bostina, M., Konstantinopoulos, P. A., Fountzilas, H., … Wulf, G. M. (2009). Altered proliferation and differentiation properties of primary mammary epithelial cells from BRCA1 mutation carriers. Cancer Research, 69(4), 1273-1278. doi: 10.1158/0008-5472.can-08-2954

Journal - Research Article

Bostina, M., Bubeck, D., Schwartz, C., Nicastro, D., Filman, D. J., & Hogle, J. M. (2007). Single particle cryoelectron tomography characterization of the structure and structural variability of poliovirus-receptor-membrane complex at 30 Å resolution. Journal of Structural Biology, 160(2), 200-210. doi: 10.1016/j.jsb.2007.08.009

Journal - Research Article

Peng, G., Bostina, M., Radermacher, M., Rais, I., Karas, M., & Michel, H. (2006). Biochemical and electron microscopic characterization of the F1F0 ATP synthase from the hyperthermophilic eubacterium Aquifex aeolicus. FEBS Letters, 580(25), 5934-5940. doi: 10.1016/j.febslet.2006.09.062

Journal - Research Article

Bostina, M., Mohsin, B., Kühlbrandt, W., & Collinson, I. (2005). Atomic model of the E. coli membrane-bound protein translocation complex SecYEG. Journal of Molecular Biology, 352(5), 1035-1043. doi: 10.1016/j.jmb.2005.08.005

Journal - Research Article

Raunser, S., Hasse, W., Bostina, M., Parcej, D. N., & Kühlbrandt, W. (2005). High-yield expression, reconstitution and structure of the recombinant, fully functional glutamate transporter GLT-1 from Rattus norvegicus. Journal of Molecular Biology, 351(3), 598-613. doi: 10.1016/j.jmb.2005.06.036

Journal - Research Article

Abdrakhmanova, A., Zickermann, V., Bostina, M., Radermacher, M., Schägger, H., Kerscher, S., & Brandt, U. (2004). Subunit composition of mitochondrial complex I from the yeast Yarrowia lipolytica. Biochimica et Biophysica Acta: Bioenergetics, 1658(1-2), 148-156. doi: 10.1016/j.bbabio.2004.04.019

Journal - Research Article

Peng, G., Fritzsch, G., Zickermann, V., Schägger, H., Mentele, R., Lottspeich, F., Bostina, M., … Michel, H. (2003). Isolation, characterization and electron microscopic single particle analysis of the NADH:Ubiquinone oxidoreductase (complex I) from the hyperthermophilic eubacterium Aquifex aeolicus. Biochemistry, 42(10), 3032-3039. doi: 10.1021/bi026876v

Journal - Research Article

Zickermann, V., Bostina, M., Hunte, C., Ruiz, T., Radermacher, M., & Brandt, U. (2003). Functional implications from an unexpected position of the 49-kDa subunit of NADH:Ubiquinone oxidoreductase. Journal of Biological Chemistry, 278(31), 29072-29078. doi: 10.1074/jbc.M302713200

Journal - Research Article

Velamoor, S., Mitchell, A., Bostina, M., & Harland, D. (2021). Processing hair follicles for transmission electron microscopy. Experimental Dermatology. Advance online publication. doi: 10.1111/exd.14439

Journal - Research Other

Jayawardena, N., Poirier, J. T., Burga, L. N., & Bostina, M. (2020). Virus-receptor interactions and virus neutralization: Insights for oncolytic virus development. Oncolytic Virotherapy, 9, 1-15. doi: 10.2147/ov.S186337

Journal - Research Other

Bostina, M. (2019). Monoclonal antibodies point to Achilles' heel in picornavirus capsid. PLoS Biology, 17(4), e3000232. doi: 10.1371/journal.pbio.3000232

Journal - Research Other

Jayawardena, N., Burga, L. N., Poirier, J. T., & Bostina, M. (2019). Virus-receptor interactions: Structural insights for oncolytic virus development. Oncolytic Virotherapy, 8, 39-56. doi: 10.2147/ov.S218494

Journal - Research Other

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