Associate Professor Mihnea Bostina
Position | Associate Professor and Academic Director, Otago Centre for Electron Microscopy |
---|---|
Department | Department of Microbiology and Immunology |
Qualifications | MSc PhD |
Research summary | Structural biology, viral pathogenesis and molecular virology |
Research
Our research interests are in understanding molecular architectures and relating those structures to their functional mechanisms. To do this, we employ cryo-electron microscopy and electron tomography combined with image analysis techniques.Publications
Harfoot, R., Lawley, B., Hernández, L. C., Kuang, J., Grant, J., Treece, J. M., LeQueux, S., Day, R., Jack, S., Stanton, J.-A. L., Bostina, M., Ussher, J. E., & Quiñones‑Mateu, M. E. (2022). Characterization of the first SARS-CoV-2 isolates from Aotearoa New Zealand as part of a rapid response to the COVID-19 pandemic. Viruses, 14, 366. doi: 10.3390/v14020366
Wang, I., Gupta, S. K., Ems, G., Jayawardena, N., Strauss, M., & Bostina, M. (2022). Cryo-EM structure of a possum enterovirus. Viruses, 318. Advance online publication. doi: 10.3390/v14020318
Velamoor, S., Mitchell, A., Bostina, M., & Harland, D. (2021). Processing hair follicles for transmission electron microscopy. Experimental Dermatology. Advance online publication. doi: 10.1111/exd.14439
Jayawardena, N., Miles, L. A., Burga, L. N., Rudin, C., Wolf, M., Poirier, J. T., & Bostina, M. (2021). N-linked glycosylation on anthrax toxin receptor 1 is essential for Seneca Valley virus infection. Viruses, 13(5), 769. doi: 10.3390/v13050769
Burga, L., & Bostina, M. (2021). Oncolytic Viruses. In D. H. Bamford & M. Zuckerman (Eds.), Encyclopedia of virology (Vol.1). (4th ed.) (pp. 658-661). Amsterdam, The Netherlands: Academic Press. doi: 10.1016/B978-0-12-814515-9.00064-3
Burga, L., & Bostina, M. (2021). Oncolytic Viruses. In D. H. Bamford & M. Zuckerman (Eds.), Encyclopedia of virology (Vol.1). (4th ed.) (pp. 658-661). Amsterdam, The Netherlands: Academic Press. doi: 10.1016/B978-0-12-814515-9.00064-3
Chapter in Book - Research
Levy, H., Bostina, M., Filman, D. J., & Hogle, J. M. (2010). Cell entry: A biochemical and structural perspective. In E. Ehrenfeld, E. Domingo & R. P. Roos (Eds.), The Picornaviruses. (pp. 87-104). Washington, DC: ASM Press.
Chapter in Book - Research
Harfoot, R., Lawley, B., Hernández, L. C., Kuang, J., Grant, J., Treece, J. M., LeQueux, S., Day, R., Jack, S., Stanton, J.-A. L., Bostina, M., Ussher, J. E., & Quiñones‑Mateu, M. E. (2022). Characterization of the first SARS-CoV-2 isolates from Aotearoa New Zealand as part of a rapid response to the COVID-19 pandemic. Viruses, 14, 366. doi: 10.3390/v14020366
Journal - Research Article
Wang, I., Gupta, S. K., Ems, G., Jayawardena, N., Strauss, M., & Bostina, M. (2022). Cryo-EM structure of a possum enterovirus. Viruses, 318. Advance online publication. doi: 10.3390/v14020318
Journal - Research Article
Jayawardena, N., Miles, L. A., Burga, L. N., Rudin, C., Wolf, M., Poirier, J. T., & Bostina, M. (2021). N-linked glycosylation on anthrax toxin receptor 1 is essential for Seneca Valley virus infection. Viruses, 13(5), 769. doi: 10.3390/v13050769
Journal - Research Article
Velamoor, S., Mitchell, A., Humbel, B. M., Kim, W., Pushparajan, C., Visnovsky, G., Burga, L. N., & Bostina, M. (2020). Visualizing nudivirus assembly and egress. mBio, 11(4), e01333-20. doi: 10.1128/mBio.01333-20
Journal - Research Article
Velamoor, S., Richena, M., Mitchell, A., Lequeux, S., Bostina, M., & Harland, D. (2020). High-pressure freezing followed by freeze substitution of a complex and variable density miniorgan: The wool follicle. Journal of Microscopy, 278(1), 18-28. doi: 10.1111/jmi.12875
Journal - Research Article
Harland, D. P., Novotná, V., Richena, M., Bostina, M., Velamoor, S., & McKinnon, A. J. (2019). Hair-structure mystery solved by datamining two decades of electron tomograms. Microscopy & Microanalysis, 25(Suppl. 2), 1348-1349. doi: 10.1017/S1431927619007475
Journal - Research Article
Harland, D. P., Novotna, V., Richena, M., Velamoor, S., Bostina, M., & McKinnon, A. J. (2019). Helical twist direction in the macrofibrils of keratin fibres is left handed. Journal of Structural Biology, 206, 345-348. doi: 10.1016/j.jsb.2019.04.007
Journal - Research Article
Kim, S. H., Mercer, A., Mitchell, A., de Miranda, J. R., Ward, V., Mondet, F., & Bostina, M. (2019). Viral infections alter antennal epithelium ultrastructure in honey bees. Journal of Invertebrate Pathology, 168, 107252. doi: 10.1016/j.jip.2019.107252
Journal - Research Article
McCarthy, C., Jayawardena, N., Burga, L. N., & Bostina, M. (2019). Developing picornaviruses for cancer therapy. Cancers, 11(5), 685. doi: 10.3390/cancers11050685
Journal - Research Article
Watson, B. N. J., Easingwood, R. A., Tong, B., Wolf, M., Salmond, G. P. C., Staals, R. H. J., Bostina, M., & Fineran, P. C. (2019). Different genetic and morphological outcomes for phages targeted by single or multiple CRISPR-Cas spacers. Philosophical Transactions of the Royal Society B, 374(1772), 20180090. doi: 10.1098/rstb.2018.0090
Journal - Research Article
Jayawardena, N., Burga, L. N., Easingwood, R. A., Takizawa, K., Wolf, M., & Bostina, M. (2018). Structural basis for anthrax toxin receptor 1 recognition by Seneca Valley Virus. PNAS, 115(46), E10934-E10940. doi: 10.1073/pnas.1810664115
Journal - Research Article
Strauss, M., Jayawardena, N., Sun, E., Easingwood, R. A., Burga, L. N., & Bostina, M. (2018). Cryo-electron microscopy structure of Seneca Valley Virus procapsid. Journal of Virology, 92(6), e01927-17. doi: 10.1128/jvi.01927-17
Journal - Research Article
Basu, K., Lajoie, D., Aumentado-Armstrong, T., Chen, J., Koning, R. I., Bossy, B., Bostina, M., … Rouiller, I. (2017). Molecular mechanism of DRP1 assembly studied in vitro by cryo-electron microscopy. PLoS ONE, 12(6), e0179397. doi: 10.1371/journal.pone.0179397
Journal - Research Article
Fagerlund, R. D., Wilkinson, M. E., Klykov, O., Barendregt, A., Pearce, F. G., Kieper, S. N., Maxwell, H. W. R., … Krause, K. L., Bostina, M., … Staals, R. H. J., & Fineran, P. C. (2017). Spacer capture and integration by a type I-F Cas1–Cas2-3 CRISPR adaptation complex. PNAS, 114(26), E5122-E5128. doi: 10.1073/pnas.1618421114
Journal - Research Article
Miles, L. A., Burga, L. N., Gardner, E. E., Bostina, M., Poirier, J. T., & Rudin, C. M. (2017). Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. Journal of Clinical Investigation, 127(8), 2957-2967. doi: 10.1172/jci93472
Journal - Research Article
Hampton, H. G., McNeil, M. B., Paterson, T. J., Ney, B., Williamson, N. R., Easingwood, R. A., Bostina, M., … Fineran, P. C. (2016). CRISPR-Cas gene-editing reveals RsmA and RsmC act through FlhDC to repress the SdhE flavinylation factor and control motility and prodigiosin production in Serratia. Microbiology, 162(6), 1047-1058. doi: 10.1099/mic.0.000283
Journal - Research Article
Frampton, R. A., Lopez Acedo, E., Young, V. L., Chen, D., Tong, B., Taylor, C., Easingwood, R. A., … Kleffmann, T., Bostina, M., & Fineran, P. C. (2015). Genome, proteome and structure of a T7-like bacteriophage of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae. Viruses, 7(7), 3361-3379. doi: 10.3390/v7072776
Journal - Research Article
Kalynych, S., Cherney, M., Bostina, M., Rouiller, I., & Cygler, M. (2015). Quaternary structure of WzzB and WzzE polysaccharide co-polymerases. Protein Science, 24(1), 58-69. doi: 10.1002/pro.2586
Journal - Research Article
Schoderboeck, L., Riad, S., Bokor, A. M., Wicky, H. E., Strauss, M., Bostina, M., Oswald, M. J., Empson, R. M., & Hughes, S. M. (2015). Chimeric rabies SADB19-VSVg-pseudotyped lentiviral vectors mediate long-range retrograde transduction from the mouse spinal cord. Gene Therapy, 22, 357-364. doi: 10.1038/gt.2015.3
Journal - Research Article
Schotte, L., Strauss, M., Thys, B., Halewyck, H., Filman, D. J., Bostina, M., … Rombaut, B. (2014). Mechanism of action and capsid-stabilizing properties of VHHs with an in vitro antipolioviral activity. Journal of Virology, 88(8), 4403-4413. doi: 10.1128/JVI.03402-13
Journal - Research Article
Sverzhinsky, A., Fabre, L., Cottreau, A. L., Biot-Pelletier, D. M. P., Khalil, S., Bostina, M., … Coulton, J. W. (2014). Coordinated rearrangements between cytoplasmic and periplasmic domains of the membrane protein complex ExbB-ExbD of Escherichia coli. Structure, 22(5), 791-797. doi: 10.1016/j.str.2014.02.010
Journal - Research Article
Abedi, S., Karimi, B., Kazemi, F., Bostina, M., & Vali, H. (2013). Amorphous TiO2 coated into periodic mesoporous organosilicate channels as a new binary photocatalyst for regeneration of carbonyl compounds from oximes under sunlight irradiation. Organic & Biomolecular Chemistry, 11(3), 416-419. doi: 10.1039/c2ob26907d
Journal - Research Article
Grishin, A. M., Ajamian, E., Tao, L., Bostina, M., Zhang, L., Trempe, J.-F., … Cygler, M. (2013). Family of phenylacetyl-CoA monooxygenases differs in subunit organization from other monooxygenases. Journal of Structural Biology, 184(2), 147-154. doi: 10.1016/j.jsb.2013.09.012
Journal - Research Article
Strauss, M., Levy, H. C., Bostina, M., Filman, D. J., & Hogle, J. M. (2013). RNA transfer from poliovirus 135S particles across membranes is mediated by long umbilical connectors. Journal of Virology, 87(7), 3903-3914. doi: 10.1128/jvi.03209-12
Journal - Research Article
Grishin, A. M., Ajamian, E., Zhang, L., Rouiller, I., Bostina, M., & Cygler, M. (2012). Protein-protein interactions in the β-oxidation part of the phenylacetate utilization pathway: Crystal structure of the PaaF-PaaG hydratase-isomerase complex. Journal of Biological Chemistry, 287(45), 37986-37996. doi: 10.1074/jbc.M112.388231
Journal - Research Article
Karimi, B., Behzadnia, H., Bostina, M., & Vali, H. (2012). A nano-fibrillated mesoporous carbon as an effective support for palladium nanoparticles in the aerobic oxidation of alcohols "on pure water". Chemistry: A European Journal, 18(28), 8634-8640. doi: 10.1002/chem.201200373
Journal - Research Article
Kulczyk, A. W., Akabayov, B., Lee, S.-J., Bostina, M., Berkowitz, S. A., & Richardson, C. C. (2012). An interaction between DNA polymerase and helicase is essential for the high processivity of the bacteriophage T7 replisome. Journal of Biological Chemistry, 287(46), 39050-39060. doi: 10.1074/jbc.M112.410647
Journal - Research Article
Bostina, M., Levy, H., Filman, D. J., & Hogle, J. M. (2011). Poliovirus RNA is released from the capsid near a twofold symmetry axis. Journal of Virology, 85(2), 776-783. doi: 10.1128/jvi.00531-10
Journal - Research Article
Gopalakrishnan, G., Yam, P. T., Madwar, C., Bostina, M., Rouiller, I., Colman, D. R., & Lennox, R. B. (2011). Label-free visualization of ultrastructural features of artificial synapses via cryo-EM. ACS Chemical Neuroscience, 2(12), 700-704. doi: 10.1021/cn200094j
Journal - Research Article
Levy, H. C., Bostina, M., Filman, D. J., & Hogle, J. M. (2010). Catching a virus in the act of RNA release: A novel poliovirus uncoating intermediate characterized by cryo-electron microscopy. Journal of Virology, 84(9), 4426-4441. doi: 10.1128/jvi.02393-09
Journal - Research Article
Burga, L. N., Tung, N. M., Troyan, S. L., Bostina, M., Konstantinopoulos, P. A., Fountzilas, H., … Wulf, G. M. (2009). Altered proliferation and differentiation properties of primary mammary epithelial cells from BRCA1 mutation carriers. Cancer Research, 69(4), 1273-1278. doi: 10.1158/0008-5472.can-08-2954
Journal - Research Article
Bostina, M., Bubeck, D., Schwartz, C., Nicastro, D., Filman, D. J., & Hogle, J. M. (2007). Single particle cryoelectron tomography characterization of the structure and structural variability of poliovirus-receptor-membrane complex at 30 Å resolution. Journal of Structural Biology, 160(2), 200-210. doi: 10.1016/j.jsb.2007.08.009
Journal - Research Article
Peng, G., Bostina, M., Radermacher, M., Rais, I., Karas, M., & Michel, H. (2006). Biochemical and electron microscopic characterization of the F1F0 ATP synthase from the hyperthermophilic eubacterium Aquifex aeolicus. FEBS Letters, 580(25), 5934-5940. doi: 10.1016/j.febslet.2006.09.062
Journal - Research Article
Bostina, M., Mohsin, B., Kühlbrandt, W., & Collinson, I. (2005). Atomic model of the E. coli membrane-bound protein translocation complex SecYEG. Journal of Molecular Biology, 352(5), 1035-1043. doi: 10.1016/j.jmb.2005.08.005
Journal - Research Article
Raunser, S., Hasse, W., Bostina, M., Parcej, D. N., & Kühlbrandt, W. (2005). High-yield expression, reconstitution and structure of the recombinant, fully functional glutamate transporter GLT-1 from Rattus norvegicus. Journal of Molecular Biology, 351(3), 598-613. doi: 10.1016/j.jmb.2005.06.036
Journal - Research Article
Abdrakhmanova, A., Zickermann, V., Bostina, M., Radermacher, M., Schägger, H., Kerscher, S., & Brandt, U. (2004). Subunit composition of mitochondrial complex I from the yeast Yarrowia lipolytica. Biochimica et Biophysica Acta: Bioenergetics, 1658(1-2), 148-156. doi: 10.1016/j.bbabio.2004.04.019
Journal - Research Article
Peng, G., Fritzsch, G., Zickermann, V., Schägger, H., Mentele, R., Lottspeich, F., Bostina, M., … Michel, H. (2003). Isolation, characterization and electron microscopic single particle analysis of the NADH:Ubiquinone oxidoreductase (complex I) from the hyperthermophilic eubacterium Aquifex aeolicus. Biochemistry, 42(10), 3032-3039. doi: 10.1021/bi026876v
Journal - Research Article
Zickermann, V., Bostina, M., Hunte, C., Ruiz, T., Radermacher, M., & Brandt, U. (2003). Functional implications from an unexpected position of the 49-kDa subunit of NADH:Ubiquinone oxidoreductase. Journal of Biological Chemistry, 278(31), 29072-29078. doi: 10.1074/jbc.M302713200
Journal - Research Article
Velamoor, S., Mitchell, A., Bostina, M., & Harland, D. (2021). Processing hair follicles for transmission electron microscopy. Experimental Dermatology. Advance online publication. doi: 10.1111/exd.14439
Journal - Research Other
Jayawardena, N., Poirier, J. T., Burga, L. N., & Bostina, M. (2020). Virus-receptor interactions and virus neutralization: Insights for oncolytic virus development. Oncolytic Virotherapy, 9, 1-15. doi: 10.2147/ov.S186337
Journal - Research Other
Bostina, M. (2019). Monoclonal antibodies point to Achilles' heel in picornavirus capsid. PLoS Biology, 17(4), e3000232. doi: 10.1371/journal.pbio.3000232
Journal - Research Other
Jayawardena, N., Burga, L. N., Poirier, J. T., & Bostina, M. (2019). Virus-receptor interactions: Structural insights for oncolytic virus development. Oncolytic Virotherapy, 8, 39-56. doi: 10.2147/ov.S218494
Journal - Research Other