Dr Nina Giles
| Position | Research Fellow |
|---|---|
| Department | Department of Pharmacology and Toxicology |
| Qualifications | BSc(Hons) PhD |
| Research summary | Cancer research and free radicals in biochemistry |
Research
The healthy cell needs to maintain an optimal balance of reactive oxygen species (ROS), however in many diseases states including cancer the ROS increase beyond the capability of the cell to remove them with the antioxidants available.
Our group tries to capitalise on this imbalance by introducing novel drugs which can utilise the excess ROS to destroy cancerous cells, leaving healthy cells untouched.
To achieve this we aim to try and understand a complex set of redox-sensitive pathways and reactions within cancerous cells.
Techniques employed include:
- Synthesis of novel drugs
- Cell cytotoxicity assays
- Fluorescence imaging
- Western blotting
- Nanostring gene expression sequencing
Publications
McNeill, S., Giles, N. M., Preston, D., Jones, P. P., Crowley, J. D., & Giles, G. I. (2020). The quadruply stranded metallo-supramolecular helicate [Pd2 (hextrz)4]4+ acts as a molecular mimic of cytolytic peptides. Chemical Research in Toxicology, 33, 1822-1834. doi: 10.1021/acs.chemrestox.0c00061
Zadehvakili, B., Giles, N. M., Fawcett, J. P., & Giles, G. I. (2016). Data on the catalytic mechanism of thiol peroxidase mimics [Data article]. Data in Brief, 8, 207-210. doi: 10.1016/j.dib.2016.05.037
Xian, S., Parayath, N. N., Nehoff, H., Giles, N. M., & Greish, K. (2015). The use of styrene maleic acid nanomicelles encapsulating the synthetic cannabinoid analog WIN55,212-2 for the treatment of cancer. Anticancer Research, 35(9), 4707-4712.
Aitken, J. B., Shearer, E. L., Giles, N. M., Lai, B., Vogt, B., Reboucas, J. S., … Giles, G. I. (2013). Intracellular targeting and pharmacological activity of the superoxide dismutase mimics MnTE-2-PyP5+ and MnTnHex-2-PyP5+ regulated by their porphyrin ring substituents. Inorganic Chemistry, 52(8), 4121-4123. doi: 10.1021/ic300700g
Giles, N. M., Kumari, S., Stamm, R., Patel, S., & Giles, G. I. (2012). A hydrogen peroxide electrode assay to measure thiol peroxidase activity for organoselenium and organotellurium drugs. Analytical Biochemistry, 429(2), 103-107. doi: 10.1016/j.ab.2012.07.013
McNeill, S., Giles, N. M., Preston, D., Jones, P. P., Crowley, J. D., & Giles, G. I. (2020). The quadruply stranded metallo-supramolecular helicate [Pd2 (hextrz)4]4+ acts as a molecular mimic of cytolytic peptides. Chemical Research in Toxicology, 33, 1822-1834. doi: 10.1021/acs.chemrestox.0c00061
Journal - Research Article
Xian, S., Parayath, N. N., Nehoff, H., Giles, N. M., & Greish, K. (2015). The use of styrene maleic acid nanomicelles encapsulating the synthetic cannabinoid analog WIN55,212-2 for the treatment of cancer. Anticancer Research, 35(9), 4707-4712.
Journal - Research Article
Aitken, J. B., Shearer, E. L., Giles, N. M., Lai, B., Vogt, B., Reboucas, J. S., … Giles, G. I. (2013). Intracellular targeting and pharmacological activity of the superoxide dismutase mimics MnTE-2-PyP5+ and MnTnHex-2-PyP5+ regulated by their porphyrin ring substituents. Inorganic Chemistry, 52(8), 4121-4123. doi: 10.1021/ic300700g
Journal - Research Article
Giles, N. M., Kumari, S., Gang, B. P., Yuen, C. W. W., Billaud, E. M. F., & Giles, G. I. (2012). The molecular design of S-nitrosothiols as photodynamic agents for controlled nitric oxide release. Chemical Biology & Drug Design, 80(3), 471-478. doi: 10.1111/j.1747-0285.2012.01420.x
Journal - Research Article
Giles, N. M., Kumari, S., Stamm, R., Patel, S., & Giles, G. I. (2012). A hydrogen peroxide electrode assay to measure thiol peroxidase activity for organoselenium and organotellurium drugs. Analytical Biochemistry, 429(2), 103-107. doi: 10.1016/j.ab.2012.07.013
Journal - Research Article
Oh, J. Y., Giles, N., Landar, A., & Darley-Usmar, V. (2008). Accumulation of 15-deoxy-Δ12,14-prostaglandin J2 adduct formation with Keap1 over time: Effects on potency for intracellular antioxidant defense induction. Biochemical Journal, 411(2), 297-306. doi: 10.1042/bj20071189
Journal - Research Article
Landar, A., Giles, N., Zmijewski, J. W., Watanabe, N., Oh, J.-Y., & Darley-Usmar, V. M. (2006). Modification of lipids by reactive oxygen and nitrogen species: The oxy-nitroxy-lipidome and its role in redox cell signaling. Future Lipidology, 1(2), 203-211. doi: 10.2217/17460875.1.2.203
Journal - Research Article
Landar, A., Oh, J.-Y., Giles, N. M., Isom, A., Kirk, M., Barnes, S., & Darley-Usmar, V. M. (2006). A sensitive method for the quantitative measurement of protein thiol modification in response to oxidative stress. Free Radical Biology & Medicine, 40(3), 459-468. doi: 10.1016/j.freeradbiomed.2005.08.046
Journal - Research Article
Fry, F. H., Holme, A. L., Giles, N. M., Giles, G. I., Collins, C., Holt, K., … Jacob, C. (2005). Multifunctional redox catalysts as selective enhancers of oxidative stress. Organic & Biomolecular Chemistry, 3(14), 2579-2587. doi: 10.1039/b502197a
Journal - Research Article
Beers, S. A., Buckland, A. G., Giles, N., Gelb, M. H., & Wilton, D. C. (2003). Effect of tryptophan insertions on the properties of the human group IIA phospholipase A2: Mutagenesis produces an enzyme with characteristics similar to those of the human group V phospholipase A2. Biochemistry, 42, 7326-7338. doi: 10.1021/bi0343222
Journal - Research Article
Giles, N. M., Giles, G. I., & Jacob, C. (2003). Multiple roles of cysteine in biocatalysis. Biochemical & Biophysical Research Communications, (1), 1-4. doi: 10.1016/S0006-291X(02)02770-5
Journal - Research Article
Giles, N. M., Giles, G. I., Holley, J. E., Gutowski, N. J., & Jacob, C. (2003). Targeting oxidative stress-related diseases: Organochalcogen catalysts as redox sensitizers. Biochemical Pharmacology, 66(10), 2021-2028. doi: 10.1016/S0006-2952(03)00544-6
Journal - Research Article
Giles, N. M., Gutowski, N. J., Giles, G. I., & Jacob, C. (2003). Redox catalysts as sensitisers towards oxidative stress. FEBS Letters, 535, 179-182. doi: 10.1016/S0014-5793(02)03890-5
Journal - Research Article
Giles, N. M., Watts, A. B., Giles, G. I., Fry, F. H., Littlechild, J. A., & Jacob, C. (2003). Metal and redox modulation of cysteine protein function. Chemistry & Biology, 10(8), 677-693. doi: 10.1016/S1074-5521(03)00174-1
Journal - Research Article
Jacob, C., Giles, G. I., Giles, N. M., & Sies, H. (2003). Sulfur and selenium: The role of oxidation state in protein structure and function. Angewandte Chemie International Edition, 42(39), 4742-4758. doi: 10.1002/anie.200300573
Journal - Research Article
Giles, G. I., Tasker, K. M., Collins, C., Giles, N. M., O'Rourke, E., & Jacob, C. (2002). Reactive sulphur species: An in vitro investigation of the oxidation properties of disulphide S-oxides. Biochemical Journal, 364, 579-585.
Journal - Research Article
Zadehvakili, B., Giles, N. M., Fawcett, J. P., & Giles, G. I. (2016). Data on the catalytic mechanism of thiol peroxidase mimics [Data article]. Data in Brief, 8, 207-210. doi: 10.1016/j.dib.2016.05.037
Journal - Research Other
Giles, G. I., Giles, N. M., Collins, C. A., Holt, K., Fry, F. H., Lowden, P. A. S., … Jacob, C. (2003). Electrochemical, in vitro and cell culture analysis of integrated redox catalysts: Implications for cancer therapy. Chemical Communications, 2030-2031. doi: 10.1039/b302877a
Journal - Research Other
