Professor Peter Fineran
Position | Professor |
---|---|
Department | Department of Microbiology and Immunology |
Qualifications | BSc (Hons) PhD |
Research summary | Molecular microbiology and bacteriophages |
Research
Our research goal is to understand the interactions between mobile genetic elements, such as bacteriophages and plasmids, and their bacterial hosts. Our main interest is in phage resistance mechanisms.
The page-bacterium ‘arms race’
Accumulating evidence demonstrates that viruses are the most abundant biological entities on the planet. They outnumber bacteria by approximately 10 to 1 and there are an estimated >1030 tailed bacteriophages globally! The result is an obvious selective pressure for bacteria to acquire resistance to phage attack and for phages to acquire counter defence mechanisms. Indeed, many mechanisms of phage resistance are known, ranging from bacterial cell surface alterations, through restriction and modification, to abortive infection. Our research focuses on:
- Abortive infection and toxin-antitoxin 'innate immune systems'
- CRISPR-Cas 'adaptive immune systems'
- Phages as antimicrobial agents
Publications
McBride, T. M., Cameron, S. C., Fineran, P. C., & Fagerlund, R. D. (2023). The biology and type I/III hybrid nature of type I-D CRISPR-Cas systems. Biochemical Journal, 480(7), 471-488. doi: 10.1042/bcj20220073
Mahler, M., Malone, L. M., van den Berg, D. F., Smith, L. M., Brouns, S. J. J., & Fineran, P. C. (2023). An OmpW-dependent T4-like phage infects Serratia sp. ATCC 39006. Microbial Genomics, 9(3), 000968. doi: 10.1099/mgen.0.000968
Smith, L. M., & Fineran, P. C. (2023). Phage capsid recognition triggers activation of a bacterial toxin-antitoxin defense system [Spotlight]. Molecular Cell, 83, 165-166. doi: 10.1016/j.molcel.2022.12.020
Mahler, M., Costa, A. R., van Beljouw, S. P. B., Fineran, P. C., & Brouns, S. J. J. (2023). Approaches for bacteriophage genome engineering [Review]. Trends in Biotechnology, 41(5), 669-685. doi: 10.1016/j.tibtech.2022.08.008
Mayo-Muñoz, D., Smith, L. M., Garcia-Doval, C., Malone, L. M., Harding, K. R., Jackson, S. A., Hampton, H. G., Fagerlund, R. D., Gumy, L. F., & Fineran, P. C. (2022). Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection. Molecular Cell. Advance online publication. doi: 10.1016/j.molcel.2022.10.028
Lundgren, M., Charpentier, E., & Fineran, P. C. (Eds.). (2015). CRISPR: Methods and protocols: Methods in molecular biology (Vol. 1311). Humana Press, 366p. doi: 10.1007/978-1-4939-2687-9
Edited Book - Research
Bergmann, J. M., Fineran, P. C., Petty, N. K., & Salmond, G. P. C. (2019). Transduction: The transfer of host DNA by bacteriophages. In T. M. Schmidt (Ed.), Encyclopedia of microbiology. (pp. 458-473). Academic Press. doi: 10.1016/B978-0-12-801238-3.11350-9
Chapter in Book - Research
Fagerlund, R. D., Ferguson, T. J., Maxwell, H. W. R., Opel-Reading, H. K., Krause, K. L., & Fineran, P. C. (2019). Reconstitution of CRISPR adaptation in vitro and its detection by PCR. In S. Bailey (Ed.), Methods in enzymology (Vol. 616: CRISPR-Cas enzymes). (pp. 411-433). Cambridge, MA: Academic Press. doi: 10.1016/bs.mie.2018.10.024
Chapter in Book - Research
Biswas, A., Fineran, P. C., & Brown, C. M. (2015). Computational detection of CRISPR/crRNA targets. In M. Lundgren, E. Charpentier & P. C. Fineran (Eds.), CRISPR: Methods in molecular biology (Vol. 1311). (pp. 77-89). New York, NY: Humana Press. doi: 10.1007/978-1-4939-2687-9_5
Chapter in Book - Research
Fineran, P. C., Petty, N. K., & Salmond, G. P. C. (2009). Transduction: Host DNA transfer by bacteriophages. In M. Schaechter (Ed.), Encyclopedia of microbiology (Vol. 3). (3rd ed.) (pp. 666-679). Oxford, UK: Elsevier.
Chapter in Book - Research
Mahler, M., Costa, A. R., van Beljouw, S. P. B., Fineran, P. C., & Brouns, S. J. J. (2023). Approaches for bacteriophage genome engineering [Review]. Trends in Biotechnology, 41(5), 669-685. doi: 10.1016/j.tibtech.2022.08.008
Journal - Research Article
Mahler, M., Malone, L. M., van den Berg, D. F., Smith, L. M., Brouns, S. J. J., & Fineran, P. C. (2023). An OmpW-dependent T4-like phage infects Serratia sp. ATCC 39006. Microbial Genomics, 9(3), 000968. doi: 10.1099/mgen.0.000968
Journal - Research Article
Birkholz, N., Jackson, S. A., Fagerlund, R. D., & Fineran, P. C. (2022). A mobile restriction-modification system provides phage defence and resolves an epigenetic conflict with an antagonistic endonuclease. Nucleic Acids Research, 50(6), 3348-3361. doi: 10.1093/nar/gkac147
Journal - Research Article
Kim, G. E., Lee, S. Y., Birkholz, N., Kamata, K., Jeong, J.-H., Kim, Y.-G., Fineran, P. C., & Park, H. H. (2022). Molecular basis of dual anti-CRISPR and auto-regulatory functions of AcrIF24. Nucleic Acids Research, 50(19), 11344-11358. doi: 10.1093/nar/gkac880
Journal - Research Article
Lee, S. Y., Birkholz, N., Fineran, P. C., & Park, H. H. (2022). Molecular basis of anti-CRISPR operon repression by Aca10. Nucleic Acids Research. Advance online publication. doi: 10.1093/nar/gkac656
Journal - Research Article
Malone, L. M., Hampton, H. G., Morgan, X. C., & Fineran, P. C. (2022). Type I CRISPR-Cas provides robust immunity but incomplete attenuation of phage-induced cellular stress. Nucleic Acids Research, 50(1), 160-174. doi: 10.1093/nar/gkab1210
Journal - Research Article
Mayo-Muñoz, D., Smith, L. M., Garcia-Doval, C., Malone, L. M., Harding, K. R., Jackson, S. A., Hampton, H. G., Fagerlund, R. D., Gumy, L. F., & Fineran, P. C. (2022). Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection. Molecular Cell. Advance online publication. doi: 10.1016/j.molcel.2022.10.028
Journal - Research Article
Payne, L. J., Meaden, S., Mestre, M. R., Palmer, C., Toro, N., & Fineran, P. C. (2022). PADLOC: A web server for the identification of antiviral defence systems in microbial genomes. Nucleic Acids Research, gkac400. Advance online publication. doi: 10.1093/nar/gkac400
Journal - Research Article
Pinilla-Redondo, R., Russell, J., Mayo-Muñoz, D., Shah, S. A., Garrett, R. A., Nesme, J., … Fineran, P. C., & Sørensen, S. J. (2022). CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids. Nucleic Acids Research, 50(8), 4315-4328. doi: 10.1093/nar/gkab859
Journal - Research Article
Schwartz, E. A., McBride, T. M., Bravo, J. P. K., Wrapp, D., Fineran, P. C., Fagerlund, R. D., & Taylor, D. W. (2022). Structural rearrangements allow nucleic acid discrimination by type I-D Cascade. Nature Communications, 13, 2829. doi: 10.1038/s41467-022-30402-8
Journal - Research Article
Shehreen, S., Birkholz, N., Fineran, P. C., & Brown, C. M. (2022). Widespread repression of anti-CRISPR production by anti-CRISPR-associated proteins. Nucleic Acids Research. Advance online publication. doi: 10.1093/nar/gkac674
Journal - Research Article
Warring, S. L., Malone, L. M., Jayaraman, J., Easingwood, R. A., Rigano, L. A., Frampton, R. A., … Bostina, M., & Fineran, P. C. (2022). A lipopolysaccharide-dependent phage infects a pseudomonad phytopathogen and can evolve to evade phage resistance. Environmental Microbiology, 24(10), 4834-4852. doi: 10.1111/1462-2920.16106
Journal - Research Article
Payne, L. J., Todeschini, T. C., Wu, Y., Perry, B. J., Ronson, C. W., Fineran, P. C., … Jackson, S. A. (2021). Identification and classification of antiviral defence systems in bacteria and archaea with PADLOC reveals new system types. Nucleic Acids Research, 49(19), 10868-10878. doi: 10.1093/nar/gkab883
Journal - Research Article
Rey Campa, A., Smith, L. M., Hampton, H. G., Sharma, S., Jackson, S. A., Bischler, T., … Fineran, P. C. (2021). The Rsm (Csr) post-transcriptional regulatory pathway coordinately controls multiple CRISPR-Cas immune systems. Nucleic Acids Research, 49(16), 9508-9525. doi: 10.1093/nar/gkab704
Journal - Research Article
Sitter, T. L., Vaughan, A. L., Schoof, M., Jackson, S. A., Glare, T. R., Cox, M. P., Fineran, P. C., Gardner, P. P., & Hurst, M. R. H. (2021). Evolution of virulence in a novel family of transmissible mega-plasmids. Environmental Microbiology, 23(9), 5289-5304. doi: 10.1111/1462-2920.15595
Journal - Research Article
Smith, L. M., Jackson, S. A., Malone, L. M., Ussher, J. E., Gardner, P. P., & Fineran, P. C. (2021). The Rcs stress response inversely controls surface and CRISPR–Cas adaptive immunity to discriminate plasmids and phages. Nature Microbiology, 6, 162-172. doi: 10.1038/s41564-020-00822-7
Journal - Research Article
Usher, B., Birkholz, N., Beck, I. N., Fagerlund, R. D., Jackson, S. A., Fineran, P. C., & Blower, T. R. (2021). Crystal structure of the anti-CRISPR repressor Aca2. Journal of Structural Biology, 213, 107752. doi: 10.1016/j.jsb.2021.107752
Journal - Research Article
Beck, I. N., Usher, B., Hampton, H. G., Fineran, P. C., & Blower, T. R. (2020). Antitoxin autoregulation of M. tuberculosis toxin-antitoxin expression through negative cooperativity arising from multiple inverted repeat sequences. Biochemical Journal, 477, 2401-2419. doi: 10.1042/bcj20200368
Journal - Research Article
Cai, Y., Usher, B., Gutierrez, C., Tolcan, A., Mansour, M., Fineran, P. C., … Blower, T. R. (2020). A nucleotidyltransferase toxin inhibits growth of Mycobacterium tuberculosis through inactivation of tRNA acceptor stems. Science Advances, 6(31), eabb6651. doi: 10.1126/sciadv.abb6651
Journal - Research Article
Hampton, H. G., Smith, L. M., Ferguson, S., Meaden, S., Jackson, S. A., & Fineran, P. C. (2020). Functional genomics reveals the toxin-antitoxin repertoire and AbiE activity in Serratia. Microbial Genomics, 6, 1-15. doi: 10.1099/mgen.0.000458
Journal - Research Article
Jayaraman, J., Jones, W. T., Harvey, D., Hemara, L. M., McCann, H. C., Yoon, M., Warring, S. L., Fineran, P. C., … Templeton, M. D. (2020). Variation at the common polysaccharide antigen locus drives lipopolysaccharide diversity within the Pseudomonas syringae species complex. Environmental Microbiology, 22(12), 5356-5372. doi: 10.1111/1462-2920.15250
Journal - Research Article
Malone, L. M., Warring, S. L., Jackson, S. A., Warnecke, C., Gardner, P. P., Gumy, L. F., & Fineran, P. C. (2020). A jumbo phage that forms a nucleus-like structure evades CRISPR-Cas DNA targeting but is vulnerable to type III RNA-based immunity. Nature Microbiology, 5, 48-55. doi: 10.1038/s41564-019-0612-5
Journal - Research Article
McBride, T. M., Schwartz, E. A., Kumar, A., Taylor, D. W., Fineran, P. C., & Fagerlund, R. D. (2020). Diverse CRISPR-Cas complexes require independent translation of small and large subunits from a single gene. Molecular Cell, 80, 971-979. doi: 10.1016/j.molcel.2020.11.003
Journal - Research Article
Pinilla-Redondo, R., Shehreen, S., Marino, N. D., Fagerlund, R. D., Brown, C. M., Sørensen, S. J., Fineran, P. C., & Bondy-Denomy, J. (2020). Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements. Nature Communications, 11(1), 5652. doi: 10.1038/s41467-020-19415-3
Journal - Research Article
Rollie, C., Chevallereau, A., Watson, B. N. J., Chyou, T.-y., Fradet, O., McLeod, I., Fineran, P. C., Brown, C. M., … Westra, E. R. (2020). Targeting of temperate phages drives loss of type I CRISPR-Cas systems. Nature, 578, 149-153. doi: 10.1038/s41586-020-1936-2
Journal - Research Article
Birkholz, N., Fagerlund, R. D., Smith, L. M., Jackson, S. A., & Fineran, P. C. (2019). The autoregulator Aca2 mediates anti-CRISPR repression. Nucleic Acids Research, 47(18), 9658-9665. doi: 10.1093/nar/gkz721
Journal - Research Article
Edwards, R. A., Vega, A. A., Norman, H. M., Ohaeri, M., Levi, K., Dinsdale, E. A., … Fineran, P. C., … Dutilh, B. E. (2019). Global phylogeography and ancient evolution of the widespread human gut virus crAssphage. Nature Microbiology, 4, 1727-1736. doi: 10.1038/s41564-019-0494-6
Journal - Research Article
Fineran, P. C. (2019). CRISPR-Cas impedes archaeal mating. Nature Microbiology, 4(1), 2-3. doi: 10.1038/s41564-018-0326-0
Journal - Research Article
Greisch, J.-F., Tamara, S., Scheltema, R. A., Maxwell, H. W. R., Fagerlund, R. D., Fineran, P. C., … Heck, A. J. R. (2019). Expanding the mass range for UVPD-based native top-down mass spectrometry. Chemical Science, 10, 7163-7171. doi: 10.1039/c9sc01857c
Journal - Research Article
Hampton, H. G., Patterson, A. G., Chang, J. T., Taylor, C., & Fineran, P. C. (2019). GalK limits type I-F CRISPR-Cas expression in a CRP-dependent manner. FEMS Microbiology Letters, 366, fnz137. doi: 10.1093/femsle/fnz137
Journal - Research Article
Jackson, S. A., Birkholz, N., Malone, L. M., & Fineran, P. C. (2019). Imprecise spacer acquisition generates CRISPR-Cas immune diversity through primed adaptation. Cell Host & Microbe, 25, 250-260. doi: 10.1016/j.chom.2018.12.014
Journal - Research Article
Nicholson, T. J., Jackson, S. A., Croft, B. I., Staals, R. H. J., Fineran, P. C., & Brown, C. M. (2019). Bioinformatic evidence of widespread priming in type I and II CRISPR-Cas systems. RNA Biology, 16(4), 566-576. doi: 10.1080/15476286.2018.1509662
Journal - Research Article
Shehreen, S., Chyou, T.-y., Fineran, P. C., & Brown, C. M. (2019). Genome-wide correlation analysis suggests different roles of CRISPR-Cas systems in the acquisition of antibiotic resistance genes in diverse species. Philosophical Transactions of the Royal Society B, 374(1772), 20180384. doi: 10.1098/rstb.2018.0384
Journal - Research Article
Watson, B. N. J., Easingwood, R. A., Tong, B., Wolf, M., Salmond, G. P. C., Staals, R. H. J., Bostina, M., & Fineran, P. C. (2019). Different genetic and morphological outcomes for phages targeted by single or multiple CRISPR-Cas spacers. Philosophical Transactions of the Royal Society B, 374(1772), 20180090. doi: 10.1098/rstb.2018.0090
Journal - Research Article
Watson, B. N. J., Vercoe, R. B., Salmond, G. P. C., Westra, E. R., Staals, R. H. J., & Fineran, P. C. (2019). Type I–F CRISPR-Cas resistance against virulent phages results in abortive infection and provides population-level immunity. Nature Communications, 10, 5526. doi: 10.1038/s41467-019-13445-2
Journal - Research Article
Dy, R. L., Rigano, L. A., & Fineran, P. C. (2018). Phage-based biocontrol strategies and their application in agriculture and aquaculture. Biochemical Society Transactions, 46(4), BST20180178, 1605-1613. doi: 10.1042/bst20180178
Journal - Research Article
Hampton, H. G., Jackson, S. A., Fagerlund, R. D., Vogel, A. I. M., Dy, R. L., Blower, T. R., & Fineran, P. C. (2018). AbiEi binds cooperatively to the Type IV abiE toxin-antitoxin operator via a positively-charged surface and causes DNA bending and negative autoregulation. Journal of Molecular Biology, 430(8), 1141-1156. doi: 10.1016/j.jmb.2018.02.022
Journal - Research Article
Watson, B. N. J., Staals, R. H. J., & Fineran, P. C. (2018). CRISPR-Cas-mediated phage resistance enhances horizontal gene transfer by transduction. mBio, 9(1), e02406-17. doi: 10.1128/mBio.02406-17
Journal - Research Article
Blower, T. R., Chai, R., Przybilski, R., Chindhy, S., Fang, X., Kidman, S. E., … Fineran, P. C., & Salmond, G. P. C. (2017). Evolution of Pectobacterium bacteriophage ΦM1 to escape two bifunctional type III toxin-antitoxin and abortive infection systems through mutations in a single viral gene. Applied & Environmental Microbiology, 83(8), e03229-16. doi: 10.1128/aem.03229-16
Journal - Research Article
Fagerlund, R. D., Wilkinson, M. E., Klykov, O., Barendregt, A., Pearce, F. G., Kieper, S. N., Maxwell, H. W. R., … Krause, K. L., Bostina, M., … Staals, R. H. J., & Fineran, P. C. (2017). Spacer capture and integration by a type I-F Cas1–Cas2-3 CRISPR adaptation complex. PNAS, 114(26), E5122-E5128. doi: 10.1073/pnas.1618421114
Journal - Research Article
Jackson, S. A., McKenzie, R. E., Fagerlund, R. D., Kieper, S. N., Fineran, P. C., & Brouns, S. J. J. (2017). CRISPR-Cas: Adapting to change. Science, 356(6333), eaal5056. doi: 10.1126/science.aal5056
Journal - Research Article
Mesarich, C. H., Rees-George, J., Gardner, P. P., Ghomi, F. A., Gerth, M. L., Andersen, M. T., … Fineran, P. C., & Templeton, M. D. (2017). Transposon insertion libraries for the characterization of mutants from the kiwifruit pathogen Pseudomonas syringae pv. actinidiae. PLoS ONE, 12(3), e0172790. doi: 10.1371/journal.pone.0172790
Journal - Research Article
Silas, S., Lucas-Elio, P., Jackson, S. A., Aroca-Crevillén, A., Hansen, L. L., Fineran, P. C., … Sánchez-Amat, A. (2017). Type III CRISPR-Cas systems can provide redundancy to counteract viral escape from type I systems. eLIFE, 6, e27601. doi: 10.7554/eLife.27601
Journal - Research Article
Biswas, A., Staals, R. H. J., Morales, S. E., Fineran, P. C., & Brown, C. M. (2016). CRISPRDetect: A flexible algorithm to define CRISPR arrays. BMC Genomics, 17, 356. doi: 10.1186/s12864-016-2627-0
Journal - Research Article
Hampton, H. G., McNeil, M. B., Paterson, T. J., Ney, B., Williamson, N. R., Easingwood, R. A., Bostina, M., … Fineran, P. C. (2016). CRISPR-Cas gene-editing reveals RsmA and RsmC act through FlhDC to repress the SdhE flavinylation factor and control motility and prodigiosin production in Serratia. Microbiology, 162(6), 1047-1058. doi: 10.1099/mic.0.000283
Journal - Research Article
Panda, P., Vanga, B. R., Lu, A., Fiers, M., Fineran, P. C., Butler, R., … Ronson, C. W., & Pitman, A. R. (2016). Pectobacterium atrosepticum and Pectobacterium carotovorum harbor distinct, independently acquired integrative and conjugative elements encoding coronafacic acid that enhance virulence on potato stems. Frontiers in Microbiology, 7, 397. doi: 10.3389/fmicb.2016.00397
Journal - Research Article
Patterson, A. G., Jackson, S. A., Taylor, C., Evans, G. B., Salmond, G. P. C., Przybilski, R., Staals, R. H. J., & Fineran, P. C. (2016). Quorum sensing controls adaptive immunity through the regulation of multiple CRISPR-Cas systems. Molecular Cell, 64(6), 1102-1108. doi: 10.1016/j.molcel.2016.11.012
Journal - Research Article
Pawluk, A., Staals, R. H. J., Taylor, C., Watson, B. N. J., Saha, S., Fineran, P. C., … Davidson, A. R. (2016). Inactivation of CRISPR-Cas systems by anti-CRISPR proteins in diverse bacterial species. Nature Microbiology, 1, 16085. doi: 10.1038/nmicrobiol.2016.85
Journal - Research Article
Staals, R. H. J., Jackson, S. A., Biswas, A., Brouns, S. J. J., Brown, C. M., & Fineran, P. C. (2016). Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system. Nature Communications, 7, 12853. doi: 10.1038/ncomms12853
Journal - Research Article
Wilkinson, M. E., Nakatani, Y., Staals, R. H. J., Kieper, S. N., Opel-Reading, H. K., McKenzie, R. E., Fineran, P. C., & Krause, K. L. (2016). Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system. Biochemical Journal, 473, 1063-1072. doi: 10.1042/bcj20160078
Journal - Research Article
Fagerlund, R. D., Staals, R. H. J., & Fineran, P. C. (2015). The Cpf1 CRISPR-Cas protein expands genome-editing tools. Genome Biology, 16, 251. doi: 10.1186/s13059-015-0824-9
Journal - Research Article
Frampton, R. A., Lopez Acedo, E., Young, V. L., Chen, D., Tong, B., Taylor, C., Easingwood, R. A., … Kleffmann, T., Bostina, M., & Fineran, P. C. (2015). Genome, proteome and structure of a T7-like bacteriophage of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae. Viruses, 7(7), 3361-3379. doi: 10.3390/v7072776
Journal - Research Article
Holguín Moreno, A. V., Rangel, G., Clavijo, V., Prada, C., Mantilla, M., Gomez, M. C., … Fineran, P. C., … Vives, M. J. (2015). Phage ΦPan70, a putative temperate phage, controls Pseudomonas aeruginosa in planktonic, biofilm and burn mouse model assays. Viruses, 7(8), 4602-4623. doi: 10.3390/v7082835
Journal - Research Article
Patterson, A. G., Chang, J. T., Taylor, C., & Fineran, P. C. (2015). Regulation of the Type I-F CRISPR-Cas system by CRP-cAMP and GalM controls spacer acquisition and interference. Nucleic Acids Research, 43(12), 6038-6048. doi: 10.1093/nar/gkv517
Journal - Research Article
Salmond, G. P. C., & Fineran, P. C. (2015). A century of the phage: Past, present and future. Nature Reviews Microbiology, 777-786. doi: 10.1038/nrmicro3564
Journal - Research Article
Templeton, M. D., Warren, B. A., Anderson, M. T., Rikkerink, E. H. A., & Fineran, P. C. (2015). Complete DNA sequence of Pseudomonas syringae pv. actinidiae, the causal agent of kiwifruit canker disease. Genome Announcements, 3(5), e01054 -e01055 . doi: 10.1128/genomeA.01054-15
Journal - Research Article
Biswas, A., Fineran, P. C., & Brown, C. M. (2014). Accurate computational prediction of the transcribed strand of CRISPR noncoding RNAs. Bioinformatics, 30(13), 1805-1813. doi: 10.1093/bioinformatics/btu114
Journal - Research Article
Dy, R. L., Przybilski, R., Semeijn, K., Salmond, G. P. C., & Fineran, P. C. (2014). A widespread bacteriophage abortive infection system functions through a Type IV toxin-antitoxin mechanism. Nucleic Acids Research, 42(7), 4590-4605. doi: 10.1093/nar/gkt1419
Journal - Research Article
Dy, R. L., Richter, C., Salmond, G. P. C., & Fineran, P. C. (2014). Remarkable mechanisms in microbes to resist phage infections. Annual Review of Virology, 1, 307-331. doi: 10.1146/annurev-virology-031413-085500
Journal - Research Article
Fineran, P. C., & Dy, R. L. (2014). Gene regulation by engineered CRISPR-Cas systems. Current Opinion in Microbiology, 18, 83-89. doi: 10.1016/j.mib.2014.02.007
Journal - Research Article
Fineran, P. C., Gerritzen, M. J. H., Suárez-Diez, M., Künne, T., Boekhorst, J., van Hijum, S. A. F. T., Staals, R. H. J., & Brouns, S. J. J. (2014). Degenerate target sites mediate rapid primed CRISPR adaptation. PNAS, 111(16), E1629-E1638. doi: 10.1073/pnas.1400071111
Journal - Research Article
Frampton, R. A., Taylor, C., Holguín Moreno, A. V., Visnovsky, S. B., Petty, N. K., Pitman, A. R., & Fineran, P. C. (2014). Identification of bacteriophages for the biocontrol of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae. Applied & Environmental Microbiology, 80(7), 2216-2228. doi: 10.1128/aem.00062-14
Journal - Research Article
McNeil, M. B., Hampton, H. G., Hards, K. J., Watson, B. N. J., Cook, G. M., & Fineran, P. C. (2014). The succinate dehydrogenase assembly factor, SdhE, is required for the flavinylation and activation of fumarate reductase in bacteria. FEBS Letters, 588(3), 414-421. doi: 10.1016/j.febslet.2013.12.019
Journal - Research Article
Richter, C., Dy, R. L., McKenzie, R. E., Watson, B. N. J., Taylor, C., Chang, J. T., McNeil, M. B., Staals, R. H. J., & Fineran, P. C. (2014). Priming in the Type I-F CRISPR-Cas system triggers strand-independent spacer acquisition, bi-directionally from the primed protospacer. Nucleic Acids Research, 42(13), 8516-8526. doi: 10.1093/nar/gku527
Journal - Research Article
Biswas, A., Gagnon, J. N., Brouns, S. J. J., Fineran, P. C., & Brown, C. M. (2013). CRISPRTarget: Bioinformatic prediction and analysis of crRNA targets. RNA Biology, 10(5), 817-827. doi: 10.4161/rna.24046
Journal - Research Article
Cook, G. M., Robson, J. R., Frampton, R. A., McKenzie, J., Przybilski, R., Fineran, P. C., & Arcus, V. L. (2013). Ribonucleases in bacterial toxin–antitoxin systems. Biochimica et Biophysica Acta: Gene Regulatory Mechanisms, 1829, 523-531. doi: 10.1016/j.bbagrm.2013.02.007
Journal - Research Article
Dy, R. L., Pitman, A. R., & Fineran, P. C. (2013). Chromosomal targeting by CRISPR-Cas systems can contribute to genome plasticity in bacteria. Mobile Genetic Elements, 3(5), e26831. doi: 10.4161/mge.26831
Journal - Research Article
Fineran, P. C., Iglesias Cans, M. C., Ramsay, J. P., Wilf, N. M., Cossyleon, D., McNeil, M. B., … Stanton, J.-A. L., … Salmond, G. P. C. (2013). Draft genome sequence of Serratia sp. strain ATCC 39006, a model bacterium for analysis of the biosynthesis and regulation of prodigiosin, a carbapenem, and gas vesicles. Genome Announcements, 1(6), e01039-13. doi: 10.1128/genomeA.01039-13
Journal - Research Article
McNeil, M. B., & Fineran, P. C. (2013). Prokaryotic assembly factors for the attachment of flavin to complex II. Biochimica et Biophysica Acta: Bioenergetics, 1827, 637-647. doi: 10.1016/j.bbabio.2012.09.003
Journal - Research Article
McNeil, M. B., & Fineran, P. C. (2013). The conserved RGxxE motif of the bacterial FAD assembly factor SdhE is required for succinate dehydrogenase flavinylation and activity. Biochemistry, 52, 7628-7640. doi: 10.1021/bi401006a
Journal - Research Article
McNeil, M. B., Iglesias Cans, M. C., Clulow, J. S., & Fineran, P. C. (2013). YgfX (CptA) is a multimeric membrane protein that interacts with the succinate dehydrogenase assembly factor SdhE (YgfY). Microbiology, 159, 1352-1365. doi: 10.1099/mic.0.068510-0
Journal - Research Article
Richter, C., & Fineran, P. C. (2013). The subtype I-F CRISPR-Cas system influences pathogenicity island retention in Pectobacterium atrosepticum via crRNA generation and Csy complex formation. Biochemical Society Transactions, 41(6), 1468-1474. doi: 10.1042/bst20130151
Journal - Research Article
Sangal, V., Fineran, P. C., & Hoskisson, P. A. (2013). Novel configurations of type I and II CRISPR-Cas systems in Corynebacterium diphtheriae. Microbiology, 159(Pt 10), 2118-2126. doi: 10.1099/mic.0.070235-0
Journal - Research Article
Short, F. L., Pei, X. Y., Blower, T. R., Ong, S.-L., Fineran, P. C., Luisi, B. F., & Salmond, G. P. C. (2013). Selectivity and self-assembly in the control of a bacterial toxin by an antitoxic noncoding RNA pseudoknot. PNAS, 110(3), 820-821. doi: 10.1073/pnas.1216039110
Journal - Research Article
Vercoe, R. B., Chang, J. T., Dy, R. L., Taylor, C., Gristwood, T., Clulow, J. S., Richter, C., Przybilski, R., … Fineran, P. C. (2013). Cytotoxic chromosomal targeting by CRISPR/Cas systems can reshape bacterial genomes and expel or remodel pathogenicity islands. PLoS Genetics, 9(4), e1003454. doi: 10.1371/journal.pgen.1003454
Journal - Research Article
Westra, E. R., Staals, R. H. J., Gort, G., Høgh, S., Neumann, S., de la Cruz, F., Fineran, P. C., & Brouns, S. J. J. (2013). CRISPR-Cas systems preferentially target the leading regions of MOBF conjugative plasmids. RNA Biology, 10(5), 749-761. doi: 10.4161/rna.24202
Journal - Research Article
Blower, T. R., Evans, T. J., Przybilski, R., Fineran, P. C., & Salmond, G. P. C. (2012). Viral evasion of a bacterial suicide system by RNA–based molecular mimicry enables infectious altruism. PLoS Genetics, 8(10), e1003023. doi: 10.1371/journal.pgen.1003023
Journal - Research Article
Blower, T. R., Short, F. L., Fineran, P. C., & Salmond, G. P. C. (2012). Viral molecular mimicry circumvents abortive infection and suppresses bacterial suicide to make hosts permissive for replication. Bacteriophage, 2(4), 234-238. doi: 10.4161/bact.23830
Journal - Research Article
Blower, T. R., Short, F. L., Rao, F., Mizuguchi, K., Pei, X. Y., Fineran, P. C., … Salmond, G. P. C. (2012). Identification and classification of bacterial Type III toxin-antitoxin systems encoded in chromosomal and plasmid genomes. Nucleic Acids Research, 40(13), 6158-6173. doi: 10.1093/nar/gks231
Journal - Research Article
Fineran, P. C., & Charpentier, E. (2012). Memory of viral infections by CRISPR-Cas adaptive immune systems: Acquisition of new information. Virology, 434, 202-209. doi: 10.1016/j.virol.2012.10.003
Journal - Research Article
Frampton, R. A., Pitman, A. R., & Fineran, P. C. (2012). Advances in bacteriophage-mediated control of plant pathogens. International Journal of Microbiology, 2012, 326452. doi: 10.1155/2012/326452
Journal - Research Article
McNeil, M. B., Clulow, J. S., Wilf, N. M., Salmond, G. P. C., & Fineran, P. C. (2012). SdhE is a conserved protein required for the flavinylation of succinate dehydrogenase in bacteria. Journal of Biological Chemistry, 287(22), 18418-18428. doi: 10.1074/jbc.M111.293803
Journal - Research Article
Richter, C., Chang, J. T., & Fineran, P. C. (2012). Function and regulation of clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR associated (Cas) systems. Viruses, 4(10), 2291-2311. doi: 10.3390/v4102291
Journal - Research Article
Richter, C., Gristwood, T., Clulow, J. S., & Fineran, P. C. (2012). In vivo protein interactions and complex formation in the Pectobacterium atrosepticum subtype I-F CRISPR/Cas system. PLoS ONE, 7(12), e49549. doi: 10.1371/journal.pone.0049549
Journal - Research Article
Blower, T. R., Pei, X. Y., Short, F. L., Fineran, P. C., Humphreys, D. P., Luisi, B. F., & Salmond, G. P. C. (2011). A processed noncoding RNA regulates an altruistic bacterial antiviral system. Nature Structural & Molecular Biology, 18(2), 185-191. doi: 10.1038/nsmb.1981
Journal - Research Article
Gristwood, T., McNeil, M. B., Clulow, J. S., Salmond, G. P. C., & Fineran, P. C. (2011). PigS and PigP regulate prodigiosin biosynthesis in Serratia via differential control of divergent operons, which include predicted transporters of sulfur-containing molecules. Journal of Bacteriology, 193(5), 1076-1085. doi: 10.1128/JB.00352-10
Journal - Research Article
Przybilski, R., Richter, C., Gristwood, T., Clulow, J. S., Vercoe, R. B., & Fineran, P. C. (2011). Csy4 is responsible for CRISPR RNA processing in Pectobacterium atrosepticum. RNA Biology, 8(3), 517-528. doi: 10.4161/rna.8.3.15190
Journal - Research Article
Blower, T. R., Fineran, P. C., Johnson, M. J., Toth, I. K., Humphreys, D. P., & Salmond, G. P. C. (2009). Mutagenesis and functional characterization of the RNA and protein components of the toxIN abortive infection and toxin-antitoxin locus of Erwinia. Journal of Bacteriology, 191(19), 6029-6039. doi: 10.1128/JB.00720-09
Journal - Research Article
Fineran, P. C., Blower, T. R., Foulds, I. J., Humphreys, D. P., Lilley, K. S., & Salmond, G. P. C. (2009). The phage abortive infection system, toxIN, functions as a protein-RNA toxin-antitoxin pair. PNAS, 106(3), 894-899. doi: 10.1073/pnas.0808832106
Journal - Research Article
Gristwood, T., Fineran, P. C., Everson, L., Williamson, N. R., & Salmond, G. P. (2009). The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate. BMC Microbiology, 9, 112. doi: 10.1186/1471-2180-9-112
Journal - Research Article
Gristwood, T., Fineran, P. C., Everson, L., & Salmond, G. P. C. (2008). PigZ, a TetR/AcrR family repressor, modulates secondary metabolism via the expression of a putative four-component resistance-nodulation-cell-division efflux pump, ZrpADBC, in Serratia sp. ATCC 39006. Molecular Microbiology, 69(2), 418-435. doi: 10.1111/j.1365-2958.2008.06291.x
Journal - Research Article
Williamson, N. R., Fineran, P. C., Ogawa, W., Woodley, L. R., & Salmond, G. P. C. (2008). Integrated regulation involving quorum sensing, a two-component system, a GGDEF/EAL domain protein and a post-transcriptional regulator controls swarming and RhIA-dependent surfactant biosynthesis in Serratia. Environmental Microbiology, 10(5), 1202-1217. doi: 10.1111/j.1462-2920.2007.01536.x
Journal - Research Article
Fineran, P. C., Williamson, N. R., Lilley, K. S., & Salmond, G. P. C. (2007). Virulence and prodigiosin antibiotic biosynthesis in Serratia are regulated pleiotropically by the GGDEF/EAL domain protein, PigX. Journal of Bacteriology, 189(21), 7653-7662.
Journal - Research Article
Petty, N. K., Evans, T. J., Fineran, P. C., & Salmond, G. P. C. (2007). Biotechnological exploitation of bacteriophage research. Trends in Biotechnology, 25(1), 7-15.
Journal - Research Article
Williamson, N. R., Fineran, P. C., Gristwood, T., Chawrai, S. R., Leeper, F. J., & Salmond, G. P. C. (2007). Anticancer and immunosuppressive properties of bacterial prodiginines. Future Microbiology, 2(6), 605-618.
Journal - Research Article