Dr Rob Day
| Position | Research Fellow |
|---|---|
| Department | Department of Biochemistry |
| Qualifications | PhD |
| Research summary | Agricultural science, developmental biology, cancer biology, genomics and technology development |
| Memberships | Executive Committee Australasian Genomics Technology Association (AGTA) |
Research
My main research interest is the development and application of new genetic/genomics based technologies in areas of agricultural biology, developmental biology and medicine. I am best known for adapting genomics technologies for use with minute amounts of starting material to discover key imprinted genes controlling seed development in plants.
I currently use the Illumina MiSeq sequencing platform in the Centre for Translational Cancer Research and am developing new methods for sequence-based diagnostics from single cells and circulating tumor DNA. I am eager to promote and facilitate the use of genomics technology in all areas of biology and currently collaborate with several groups. Also, as way of promoting the field I have convened and/or helped organize several genomics meetings and serve on the executive committee of the Australasian Genomic Technologies Association the principal body for the promotion of genomics research in Australasia.
Additional details
Rob is Acting Operations Manager for Otago Genomics Facility.
Publications
Almomani, S. N., Alsaleh, A. A., Weeks, R. J., Chatterjee, A., Day, R. C., Honda, I., … Fukuzawa, R., Slatter, T. L., Hung, N. A., Devenish, C., Morison, I. M., & Macaulay, E. C. (2021). Identification and validation of DNA methylation changes in pre-eclampsia. Placenta, 110, 16-23. doi: 10.1016/j.placenta.2021.05.005
Aitchison, A., Hakkaart, C., Day, R. C., Morrin, H. R., Frizelle, F. A., & Keenan, J. I. (2020). APC mutations are not confined to hotspot regions in early-onset colorectal cancer. Cancers, 12, 3829. doi: 10.3390/cancers12123829
Mayyas, I. M., Weeks, R. J., Day, R. C., Magrath, H. E., O’Connor, K. M., Kardailsky, O., Hore, T. A., Hampton, M. B., & Morison, I. M. (2020). Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation. Epigenetics. Advance online publication. doi: 10.1080/15592294.2020.1861169
Begemann, A., Sticht, H., Begtrup, A., Vitobello, A., Faivre, L., Siddharth, B., … Day, R., … Sadleir, L. G., … Rauch, A. (2020). New insights into the clinical and molecular spectrum of the novel CYFIP2-related neurodevelopmental disorder and impairment of the WRC-mediated actin dynamics. Genetics in Medicine. Advance online publication. doi: 10.1038/s41436-020-01011-x
Zou, D., Day, R., Cocadiz, J. A., Parackal, S., Mitchell, W., Black, M. A., … Jackson, C., & Guilford, P. (2020). Circulating tumor DNA is a sensitive marker for routine monitoring of treatment response in advanced colorectal cancer. Carcinogenesis, 41(11), 1507-1517. doi: 10.1093/carcin/bgaa102
Day, R., & Macknight, R. (2014). Screening for imprinted genes using high-resolution melting analysis of PCR amplicons. In C. Spillane & P. C. McKeown (Eds.), Plant epigentics and epigenomics: Methods and protocols. (pp. 71-83). Humana Press. doi: 10.1007/978-1-62703-773-0_5
Chapter in Book - Research
Macknight, R. C., Herridge, R. P., & Day, R. C. (2012). Using transcriptomics to reveal gene networks of seed development in Arabidopsis. In G. K. Agrawal & R. Rakwal (Eds.), Seed development: OMICS technologies toward Improvement of seed quality and crop yield. (pp. 99-110). Dordrecht, The Netherlands: Springer. doi: 10.1007/978-94-007-4749-4_6
Chapter in Book - Research
Day, R. C. (2010). Laser microdissection of paraffin-embedded plant tissues for transcript profiling. In L. Hennig & C. Köhler (Eds.), Plant developmental biology: Methods and protocols. (pp. 321-346). New York: Spinger. doi: 10.1007/978-1-60761-765-5_22
Chapter in Book - Research
Almomani, S. N., Alsaleh, A. A., Weeks, R. J., Chatterjee, A., Day, R. C., Honda, I., … Fukuzawa, R., Slatter, T. L., Hung, N. A., Devenish, C., Morison, I. M., & Macaulay, E. C. (2021). Identification and validation of DNA methylation changes in pre-eclampsia. Placenta, 110, 16-23. doi: 10.1016/j.placenta.2021.05.005
Journal - Research Article
Aitchison, A., Hakkaart, C., Day, R. C., Morrin, H. R., Frizelle, F. A., & Keenan, J. I. (2020). APC mutations are not confined to hotspot regions in early-onset colorectal cancer. Cancers, 12, 3829. doi: 10.3390/cancers12123829
Journal - Research Article
Begemann, A., Sticht, H., Begtrup, A., Vitobello, A., Faivre, L., Siddharth, B., … Day, R., … Sadleir, L. G., … Rauch, A. (2020). New insights into the clinical and molecular spectrum of the novel CYFIP2-related neurodevelopmental disorder and impairment of the WRC-mediated actin dynamics. Genetics in Medicine. Advance online publication. doi: 10.1038/s41436-020-01011-x
Journal - Research Article
Marsman, J., Gimenez, G., Day, R. C., Horsfield, J. A., & Jones, G. T. (2020). A non-coding genetic variant associated with abdominal aortic aneurysm alters ERG gene regulation. Human Molecular Genetics, 29(4), 554-565. doi: 10.1093/hmg/ddz256
Journal - Research Article
Mayyas, I. M., Weeks, R. J., Day, R. C., Magrath, H. E., O’Connor, K. M., Kardailsky, O., Hore, T. A., Hampton, M. B., & Morison, I. M. (2020). Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation. Epigenetics. Advance online publication. doi: 10.1080/15592294.2020.1861169
Journal - Research Article
Zou, D., Day, R., Cocadiz, J. A., Parackal, S., Mitchell, W., Black, M. A., … Jackson, C., & Guilford, P. (2020). Circulating tumor DNA is a sensitive marker for routine monitoring of treatment response in advanced colorectal cancer. Carcinogenesis, 41(11), 1507-1517. doi: 10.1093/carcin/bgaa102
Journal - Research Article
Hakkaart, C., Ellison-Loschmann, L., Day, R., Sporle, A., Koea, J., Harawira, P., … Guilford, P. (2019). Germline CDH1 mutations are a significant contributor to the high frequency of early-onset diffuse gastric cancer cases in New Zealand Māori. Familial Cancer, 18(1), 83-90. doi: 10.1007/s10689-018-0080-8
Journal - Research Article
Ortega-Recalde, O., Day, R. C., Gemmell, N. J., & Hore, T. A. (2019). Zebrafish preserve global germline DNA methylation while sex-linked rDNA is amplified and demethylated during feminisation. Nature Communications, 10, 3053. doi: 10.1038/s41467-019-10894-7
Journal - Research Article
Parackal, S., Zou, D., Day, R., Black, M., & Guilford, P. (2019). Comparison of Roche Cell-Free DNA collection Tubes® to Streck Cell-Free DNA BCT®s for sample stability using healthy volunteers. Practical Laboratory Medicine, 16, e00125. doi: 10.1016/j.plabm.2019.e00125
Journal - Research Article
Clare, A. J., Day, R. C., Empson, R. M., & Hughes, S. M. (2018). Transcriptome profiling of layer 5 intratelencephalic projection neurons from the mature mouse motor cortex. Frontiers in Molecular Neuroscience, 11, 410. doi: 10.3389/fnmol.2018.00410
Journal - Research Article
Schmid, J., Day, R., Zhang, N., Dupont, P.-Y., Cox, M. P., Schardl, C. L., … Zhou, Y. (2017). Host tissue environment directs activities of an Epichloë endophyte, while it induces systemic hormone and defense responses in its native perennial ryegrass host. Molecular Plant-Microbe Interactions, 30(2), 138-149. doi: 10.1094/mpmi-10-16-0215-r
Journal - Research Article
Tantirigama, M. L. S., Oswald, M. J., Clare, A. J., Wicky, H. E., Day, R. C., Hughes, S. M., & Empson, R. M. (2016). Fezf2 expression in layer 5 projection neurons of mature mouse motor cortex. Journal of Comparative Neurology, 524(4), 829-845. doi: 10.1002/cne.23875
Journal - Research Article
Dupont, P.-Y., Eaton, C. J., Wargent, J. J., Fechtner, S., Solomon, P., Schmid, J., Day, R. C., … Cox, M. P. (2015). Fungal endophyte infection of ryegrass reprograms host metabolism and alters development. New Phytologist, 208(4), 1227-1240. doi: 10.1111/nph.13614
Journal - Research Article
Zhang, H., Luo, M., Day, R. C., Talbot, M. J., Ivanova, A., Ashton, A. R., … Macknight, R. C., … Koltunow, A. M. (2015). Developmentally regulated HEART STOPPER, a mitochondrially targeted L18 ribosomal protein gene, is required for cell division, differentiation, and seed development in Arabidopsis. Journal of Experimental Botany, 66(19), 5867-5880. doi: 10.1093/jxb/erv296
Journal - Research Article
Herridge, R. P., Day, R. C., & Macknight, R. C. (2014). The role of the MCM2-7 helicase complex during Arabidopsis seed development. Plant Molecular Biology, 86(1-2), 69-84. doi: 10.1007/s11103-014-0213-x
Journal - Research Article
Butler, M. I., Stockwell, P. A., Black, M. A., Day, R. C., Lamont, I. L., & Poulter, R. T. M. (2013). Pseudomonas syringae pv. actinidiae from recent outbreaks of kiwifruit bacterial canker belong to different clones that originated in China. PLoS ONE, 8(2), e57464. doi: 10.1371/journal.pone.0057464
Journal - Research Article
Jones, T. E. M., Day, R. C., & Beck, C. W. (2013). Attenuation of bone morphogenetic protein signaling during amphibian limb development results in the generation of stage-specific defects. Journal of Anatomy, 223(5), 474-488. doi: 10.1111/joa.12098
Journal - Research Article
Day, R. C., & Beck, C. W. (2011). Transdifferentiation from cornea to lens in Xenopus laevis depends on BMP signalling and involves upregulation of Wnt signalling. BMC Developmental Biology, 11, 54. doi: 10.1186/1471-213x-11-54
Journal - Research Article
Herridge, R. P., Day, R. C., Baldwin, S., & Macknight, R. C. (2011). Rapid analysis of seed size in Arabidopsis for mutant and QTL discovery. Plant Methods, 7. doi: 10.1186/1746-4811-7-3
Journal - Research Article
Fenby, N., Pu, H., Pennell, R., Praekelt, U., Day, R., & Scott, R. (2010). An uncoupling screen for autonomous embryo mutants in Arabidopsis thaliana. Sexual Plant Reproduction, 23, 255-264. doi: 10.1007/s00497-010-0142-z
Journal - Research Article
Day, R. C., Mueller, S., & Macknight, R. C. (2009). Identification of cytoskeleton-associated genes expressed during arabidopsis syncytial endosperm development. Plant Signaling & Behavior, 4(9), 883-886.
Journal - Research Article
Day, R. C., Herridge, R. P., Ambrose, B. A., & Macknight, R. C. (2008). Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signaling, and gene expression regulation. Plant Physiology, 148(4), 1964-1984. doi: 10.1104/pp.108.128108
Journal - Research Article
Pearl, E. J., Barker, D., Day, R. C., & Beck, C. W. (2008). Identification of genes associated with regenerative success of Xenopus laevis hindlimbs. BMC Developmental Biology, 8, 66. doi: 10.1186/1471-213X-8-66
Journal - Research Article
Day, R. C., McNoe, L. A., & MacKnight, R. C. (2007). Transcript analysis of laser microdissected plant cells. Physiologia Plantarum, 129(2), 267-282.
Journal - Research Article
Day, R. C., McNoe, L., & Macknight, R. C. (2007). Evaluation of global RNA amplification and its use for high-throughput transcript analysis of laser-microdissected endosperm. International Journal of Plant Genomics, 2007, 61028.
Journal - Research Article
Antony, J., Gimenez, G., Taylor, T., Khatoon, U., Day, R., Morison, I. M., & Horsfield, J. A. (2020). BET inhibition prevents aberrant RUNX1 and ERG transcription in STAG2 mutant leukaemia cells. Journal of Molecular Cell Biology, 12(5), 397-399. doi: 10.1093/jmcb/mjz114
Journal - Research Other
