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Otago Medical School staff profiles

Dr Khoon Lim

PositionResearch Fellow
DepartmentDepartment of Orthopaedic Surgery and Musculoskeletal Medicine (UOC)
Research summaryBioengineering; tissue regeneration

Research

Dr Khoon Lim is a biomedical engineer with specialisation in polymer chemistry. His research focus is on using a class of polymers known as hydrogels as tissue engineering matrices for a variety of applications. His research technology platform involves photo-polymerisable hydrogel bioinks for 3D bioprinting of functional tissues and also delivery of bioactive molecules to promote tissue regeneration.
  • Developing new bioinks for 3D Bioprinting of functional tissues
  • Hydrogels for smart delivery of growth factors to promote bone vascularisation
  • Engineering 3D cancer models for high-throughput drug screening
  • Delivery of brain derived growth factor for improved post-stroke recovery
  • Engineering silk as tissue engineering matrices.

Dr Lim is currently an Associate Investigator on the Centre of Research Excellence in Medical Technologies (MedTech CoRE), Affiliate Investigator on the Maurice Wilkins Centre (MWC) for Molecular Diversity and Executive Committee member of the Australasian Society of Biomaterials and Tissue Engineering (ASBTE).

Publications

Lim, K. S., Baptista, M., Moon, S., Woodfield, T. B. F., & Rnjak-Kovacina, J. (2019). Microchannels in development, survival, and vascularisation of tissue analogues for regenerative medicine. Trends in Biotechnology. Advance online publication. doi: 10.1016/j.tibtech.2019.04.004

Lim, K. S., Klotz, B. J., Lindberg, G. C. J., Melchels, F. P. W., Hooper, G. J., Malda, J., … Woodfield, T. B. F. (2019). Visible light cross-linking of gelatin hydrogels offers an enhanced cell microenvironment with improved light penetration depth. Macromolecular Bioscience. Advance online publication. doi: 10.1002/mabi.201900098

Lim, K. S., Levato, R., Costa, P. F., Castilho, M. D., Alcala-Orozco, C. R., van Dorenmalen, K. M. A., … Hooper, G. J., … Woodfield, T. B. F. (2018). Bio-resin for high resolution lithography-based biofabrication of complex cell laden constructs. Biofabrication, 10, 034101. doi: 10.1088/1758-5090/aac00c

Parker, J. D., Lim, K. S., Kieser, D. C., Woodfield, T. B. F., & Hooper, G. J. (2018). Is tranexamic acid toxic to articular cartilage when administered topically? What is the safe dose? Bone & Joint Journal, 100-B(3), 404-412. doi: 10.1302/0301-620X.100B3.BJJ-2017-1135.R1

Bertlein, S., Brown, G., Lim, K. S., Jungst, T., Boeck, T., Blunk, T., … Hooper, G. J., Woodfield, T. B. F., & Groll, J. (2017). Thiol-ene clickable gelatin: A platform bioink for multiple 3D biofabrication technologies. Advanced Materials, 29(44), 1703404. doi: 10.1002/adma.201703404

Chapter in Book - Research

Atienza Roca, P., Cui, X., Hooper, G. J., Woodfield, T. B. F., Lim, K. S., & Khang, G. (2018). Growth factor delivery systems for tissue engineering and regenerative medicine. In H. J. Chun, C. H. Park & I. K. Kwon (Eds.), Advances in experimental medicine and biology: (Vol. 1078) Cutting-edge enabling technologies for regenerative medicine. (pp. 245-269). Singapore: Springer. doi: 10.1007/978-981-13-0950-2_13

Lim, K. S., Martens, P., & Poole-Warren, L. (2018). Biosynthetic hydrogels for cell encapsulation. In J. Li, Y. Osada & J. Cooper-White (Eds.), Functional hydrogels as biomaterials. (pp. 1-29). Berlin, Germany: Springer. doi: 10.1007/978-3-662-57511-6_1

Woodfield, T., Lim, K., Morouço, P., Levato, R., Malda, J., & Melchels, F. (2017). Biofabrication in tissue engineering. In P. Ducheyne, D. W. Grainger, K. E. Healy, D. W. Hutmacher & C. J. Kirkpatrick (Eds.), Comprehensive biomaterials II: (Vol. 5) Tissue Engineering and regenerative medicine: Fundamentals. (2nd ed.) (pp. 236-266). Amsterdam, The Netherlands: Elsevier. doi: 10.1016/B978-0-12-803581-8.10221-8

Ramaswamy, Y., Lim, K. S., Zreiqat, H., & Lu, Z. (2016). Stem cells for bone regeneration: Role of trophic factors. In A. Zorzi (Ed.), Advanced techniques in bone regeneration. (pp. 357-374). Rijeka, Croatia: Intech. doi: 10.5772/63357

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Journal - Research Article

Lim, K. S., Baptista, M., Moon, S., Woodfield, T. B. F., & Rnjak-Kovacina, J. (2019). Microchannels in development, survival, and vascularisation of tissue analogues for regenerative medicine. Trends in Biotechnology. Advance online publication. doi: 10.1016/j.tibtech.2019.04.004

Cidonio, G., Alcala-Orozco, C. R., Lim, K. S., Glinka, M., Mutreja, I., Kim, Y.-H., … Woodfield, T. B. F., & Oreffo, R. O. C. (2019). Osteogenic and angiogenic tissue formation in high fidelity nanocomposite Laponite-gelatin bioinks. Biofabrication. Advance online publication. doi: 10.1088/1758-5090/ab19fd

Mutreja, I., Warring, S. L., Lim, K. S., Swadi, T., Clinch, K., Mason, J. M., … Chambers, S. T., … Gerth, M. L., … Woodfield, T. B. F. (2019). Biofilm inhibition via delivery of novel methylthioadenosine nucleosidase inhibitors from PVA-tyramine hydrogels while supporting mesenchymal stromal cell viability. ACS Biomaterials Science & Engineering, 5(2), 748-758. doi: 10.1021/acsbiomaterials.8b01141

Soltani, S., Nogaro, M. C., Rougelot, C., Newell, N., Lim, K., & Kieser, D. C. (2019). Spontaneous spinal epidural haematomas in children. European Spine Journal. Advance online publication. doi: 10.1007/s00586-019-05975-6

Lim, K. S., Klotz, B. J., Lindberg, G. C. J., Melchels, F. P. W., Hooper, G. J., Malda, J., … Woodfield, T. B. F. (2019). Visible light cross-linking of gelatin hydrogels offers an enhanced cell microenvironment with improved light penetration depth. Macromolecular Bioscience. Advance online publication. doi: 10.1002/mabi.201900098

Lindberg, G. C. J., Longoni, A., Lim, K. S., Rosenberg, A. J., Hooper, G. J., Gawlitta, D., & Woodfield, T. B. F. (2019). Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications. Acta Biomaterialia, 85, 117-130. doi: 10.1016/j.actbio.2018.12.022

Mekhileri, N. V., Lim, K. S., Brown, G. C. J., Mutreja, I., Schon, B. S., Hooper, G. J., & Woodfield, T. B. F. (2018). Automated 3D bioassembly of micro-tissues for biofabrication of hybrid tissue engineered constructs. Biofabrication, 10(2). doi: 10.1088/1758-5090/aa9ef1

Parrish, J., Lim, K. S., Baer, K., Hooper, G. J., & Woodfield, T. B. F. (2018). A 96-well microplate bioreactor platform supporting individual dual perfusion and high-throughput assessment of simple or biofabricated 3D tissue models. Lab on a Chip. Advance online publication. doi: 10.1039/c8lc00485d

Klotz, B. J., Lim, K. S., Chang, Y. X., Soliman, B. G., Pennings, I., Melchels, F. P. W., Woodfield, T. B. F., … Gawlitta, D. (2018). Engineers of a complex bone tissue model with endothelialised channels and capillary-like networks. European Cells & Materials, 35, 335-349. doi: 10.22203/eCM.v035a23

Lim, K. S., Levato, R., Costa, P. F., Castilho, M. D., Alcala-Orozco, C. R., van Dorenmalen, K. M. A., … Hooper, G. J., … Woodfield, T. B. F. (2018). Bio-resin for high resolution lithography-based biofabrication of complex cell laden constructs. Biofabrication, 10, 034101. doi: 10.1088/1758-5090/aac00c

Parker, J. D., Lim, K. S., Kieser, D. C., Woodfield, T. B. F., & Hooper, G. J. (2018). Is tranexamic acid toxic to articular cartilage when administered topically? What is the safe dose? Bone & Joint Journal, 100-B(3), 404-412. doi: 10.1302/0301-620X.100B3.BJJ-2017-1135.R1

Bertlein, S., Brown, G., Lim, K. S., Jungst, T., Boeck, T., Blunk, T., … Hooper, G. J., Woodfield, T. B. F., & Groll, J. (2017). Thiol-ene clickable gelatin: A platform bioink for multiple 3D biofabrication technologies. Advanced Materials, 29(44), 1703404. doi: 10.1002/adma.201703404

Brown, G. C. J., Lim, K. S., Farrugia, B. L., Hooper, G. J., & Woodfield, T. B. F. (2017). Covalent incorporation of heparin improves chondrogenesis in photocurable gelatin-methacryloyl hydrogels. Macromolecular Bioscience, 17(12), 1700158. doi: 10.1002/mabi.201700158

Roberts, J. J., Naudiyal, P., Lim, K. S., Poole-Warren, L. A., & Martens, P. J. (2016). A comparative study of enzyme initiators for crosslinking phenol-functionalized hydrogels for cell encapsulation. Biomaterials Research, 20, 30. doi: 10.1186/s40824-016-0077-z

Lim, K. S., Schon, B. S., Mekhileri, N. V., Brown, G. C. J., Chia, C. M., Prabakar, S., Hooper, G. J., & Woodfield, T. B. F. (2016). New visible-light photoinitiating system for improved print fidelity in gelatin-based bioinks. ACS Biomaterials Science & Engineering, 2, 1752-1762. doi: 10.1021/acsbiomaterials.6b00149

Lim, K. S., Roberts, J. J., Alves, M.-H., Poole-Warren, L. A., & Martens, P. J. (2015). Understanding and tailoring the degradation of PVA-tyramine hydrogels. Journal of Applied Polymer Science, 132(26), 42142. doi: 10.1002/app.42142

Lim, K. S., Ramaswamy, Y., Roberts, J. J., Alves, M.-H., Poole-Warren, L. A., & Martens, P. J. (2015). Promoting cell survival and proliferation in degradable poly(vinyl alcohol)-tyramine hydrogels. Macromolecular Bioscience, 15(10), 1423-1432. doi: 10.1002/mabi.201500121

Cheong, G. L. M., Lim, K. S., Jakubowicz, A., Martens, P. J., Poole-Warren, L. A., & Green, R. A. (2014). Conductive hydrogels with tailored bioactivity for implantable electrode coatings. Acta Biomaterialia, 10(3), 1216-1226. doi: 10.1016/j.actbio.2013.12.032

Peterson, M. B., Le-Masurier, S. P., Lim, K., Hook, J. M., Martens, P., & Granville, A. M. (2014). Incorporation of 5-hydroxyindazole into the self-polymerization of dopamine for novel polymer synthesis. Macromolecular Rapid Communications, 35(3), 291-297. doi: 10.1002/marc.201300746

Lim, K. S., Alves, M. H., Poole-Warren, L. A., & Martens, P. J. (2013). Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels. Biomaterials, 34(29), 7097-7105. doi: 10.1016/j.biomaterials.2013.06.005

Lim, K. S., Kundu, J., Reeves, A., Poole-Warren, L. A., Kundu, S. C., & Martens, P. J. (2012). The influence of silkworm species on cellular interactions with novel PVA/silk sericin hydrogels. Macromolecular Bioscience, 12(3), 322-332. doi: 10.1002/mabi.201100292

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Journal - Research Other

Lim, K. S., & Woodfield, T. B. F. (2015). Silk hydrogels for tissue engineering. Scripta-Ingenia, December(5), 3-7.

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