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Otago Medical School staff profiles

Dr Louisa Ashby

PositionResearch Fellow
DepartmentDepartment of Pathology and Biomedical Science (Christchurch)
QualificationsPhD
Research summaryFree radical research

Research

My research interest is focused on the biochemistry of the neutrophil, and in particular the enzymology of myeloperoxidase. This enzyme kills microbes by generating chlorine bleach, but during inflammation it can also damage healthy tissue. We are interested in the oxidation chemistry of myeloperoxidase in its microbicidal activity, and how to effectively inhibit the enzyme from causing oxidative stress in disease.

Publications

Coker, M. S., Forbes, L. V., Plowman-Holmes, M., Murdoch, D. R., Winterbourn, C. C., & Kettle, A. J. (2019). Interactions of staphyloxanthin and enterobactin with myeloperoxidase and reactive chlorine species. Archives of Biochemistry & Biophysics, 30(2), 199-206. doi: 10.1016/j.abb.2018.03.039

Forbes, L. V., & Kettle, A. J. (2018). Myeloperoxidase: Unleashing the power of hydrogen peroxide. In M. C. M. Vissers, M. B. Hampton & A. J. Kettle (Eds.), Hydrogen peroxide metabolism in health and disease. (pp. 281-304). Boca Raton, FL: CRC Press.

Turner, R., Brennan, S. O., Ashby, L. V., Dickerhof, N., Hamzah, M. R., Pearson, J. F., Stamp, L. K., & Kettle, A. J. (2018). Conjugation of urate-derived electrophiles to proteins during normal metabolism and inflammation. Journal of Biological Chemistry. Advance online publication. doi: 10.1074/jbc.RA118.005237

Albrett, A. M., Ashby, L. V., Dickerhof, N., Kettle, A. J., & Winterbourn, C. C. (2018). Heterogeneity of hypochlorous acid production in individual neutrophil phagosomes revealed by a rhodamine-based probe. Journal of Biological Chemistry. Advance online publication. doi: 10.1074/jbc.RA118.004789

Forbes, L. V., & Kettle, A. J. (2018). A multi-substrate assay for finding physiologically effective inhibitors of myeloperoxidase. Analytical Biochemistry, 544, 13-21. doi: 10.1016/j.ab.2017.12.022

Chapter in Book - Research

Forbes, L. V., & Kettle, A. J. (2018). Myeloperoxidase: Unleashing the power of hydrogen peroxide. In M. C. M. Vissers, M. B. Hampton & A. J. Kettle (Eds.), Hydrogen peroxide metabolism in health and disease. (pp. 281-304). Boca Raton, FL: CRC Press.

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Journal - Research Article

Coker, M. S., Forbes, L. V., Plowman-Holmes, M., Murdoch, D. R., Winterbourn, C. C., & Kettle, A. J. (2019). Interactions of staphyloxanthin and enterobactin with myeloperoxidase and reactive chlorine species. Archives of Biochemistry & Biophysics, 30(2), 199-206. doi: 10.1016/j.abb.2018.03.039

Turner, R., Brennan, S. O., Ashby, L. V., Dickerhof, N., Hamzah, M. R., Pearson, J. F., Stamp, L. K., & Kettle, A. J. (2018). Conjugation of urate-derived electrophiles to proteins during normal metabolism and inflammation. Journal of Biological Chemistry. Advance online publication. doi: 10.1074/jbc.RA118.005237

Forbes, L. V., & Kettle, A. J. (2018). A multi-substrate assay for finding physiologically effective inhibitors of myeloperoxidase. Analytical Biochemistry, 544, 13-21. doi: 10.1016/j.ab.2017.12.022

Albrett, A. M., Ashby, L. V., Dickerhof, N., Kettle, A. J., & Winterbourn, C. C. (2018). Heterogeneity of hypochlorous acid production in individual neutrophil phagosomes revealed by a rhodamine-based probe. Journal of Biological Chemistry. Advance online publication. doi: 10.1074/jbc.RA118.004789

Stamp, L. K., Turner, R., Khalilova, I. S., Zhang, M., Drake, J., Forbes, L. V., & Kettle, A. J. (2014). Myeloperoxidase and oxidation of uric acid in gout: Implications for the clinical consequences of hyperuricaemia. Rheumatology, 53(11), 1958-1965. doi: 10.1093/rheumatology/keu218

Kettle, A. J., Albrett, A. M., Chapman, A. L., Dickerhof, N., Forbes, L. V., Khalilova, I., & Turner, R. (2014). Measuring chlorine bleach in biology and medicine. Biochimica et Biophysica Acta: General Subjects, 1840(2), 781-793. doi: 10.1016/j.bbagen.2013.07.004

Forbes, L. V., Sjögren, T., Auchère, F., Jenkins, D. W., Thong, B., Laughton, D., … Turner, R., … Kettle, A. J. (2013). Potent reversible inhibition of myeloperoxidase by aromatic hydromates. Journal of Biological Chemistry, 288(51), 36636-36647. doi: 10.1074/jbc.M113.507756

Forbes, L. V., Furtmüller, P. G., Khalilova, I., Turner, R., Obinger, C., & Kettle, A. J. (2012). Isoniazid as a substrate and inhibitor of myeloperoxidase: Identification of amine adducts and the influence of superoxide dismutase on their formation. Biochemical Pharmacology, 84(7), 949-960. doi: 10.1016/j.bcp.2012.07.020

Tidén, A.-K., Sjögren, T., Svensson, M., Bernlind, A., Senthilmohan, R., Auchère, F., … Forbes, L. V., Magon, N. J., Paton, L. N., Jameson, G. N. L., … Kettle, A. J. (2011). 2-Thioxanthines are mechanism-based inactivators of myeloperoxidase that block oxidative stress during inflammation. Journal of Biological Chemistry, 286(43), 37578-37589. doi: 10.1074/jbc.M111.266981

Briggs, C., Kunka, S., Pennaneach, C., Forbes, L., & Machin, S. J. (2003). Performance evaluation of a new compact hematology analyzer, the Sysmex pocH-100i. Laboratory Hematology, 9(4), 225-233.

Forbes, L. V., Gale, R. E., Pizzey, A., Pouwels, K., Nathwani, A., & Linch, D. C. (2002). An activating mutation in the transmembrane domain of the granulocyte colony-stimulating factor receptor in patients with acute myeloid leukemia. Oncogene, 21(39), 5981-5989. doi: 10.1038/sj.onc.1205767

Reeves, E. P., Dekker, L. V., Forbes, L. V., Wientjes, F. B., Grogan, A., Pappin, D. J. C., & Segal, A. W. (1999). Direct interaction between p47phox and protein kinase C: Evidence for targeting of protein kinase C by p47phox in neutrophils. Biochemical Journal, 344(3), 859-866. doi: 10.1042/0264-6021:3440859

Forbes, L. V., Truong, O., Wientjes, F. B., Moss, S. J., & Segal, A. W. (1999). The major phosphorylation site of the NADPH oxidase component p67phox is Thr233. Biochemical Journal, 338(1), 99-105. doi: 10.1042/0264-6021:3380099

Leino, L., Forbes, L., Segal, A., & Cockcroft, S. (1999). Reconstitution of GTPγS-induced NADPH oxidase activity in streptolysin-O-permeabilized neutrophils by specific cytosol fractions. Biochemical & Biophysical Research Communications, 265(1), 29-37. doi: 10.1006/bbrc.1999.1631

Forbes, L. V., Moss, S. J., & Segal, A. W. (1999). Phosphorylation of p67phox in the neutrophil occurs in the cytosol and is independent of p47phox. FEBS Letters, 449(2-3), 225-229. doi: 10.1016/S0014-5793(99)00444-5

Abbott, W. G. H., Tan, P. L. J., Geursen, A., Fraser, J. D., Forbes, L., Simpson, I. J., … Skinner, M. A. (1998). TCRBV14S1 and rheumatoid arthritis revisited: Abnormalities in the percentage of transcribed TCRBV14S1 family genes in PBMC from rheumatoid arthritis patients. Scandinavian Journal of Rheumatology, 27, 68-75.

Willis, J. A., Scott, R. S., Brown, L. J., Forbes, L. V., Schmidli, R. S., Zimmet, P. Z., … Rowley, M. J. (1996). Islet cell antibodies and antibodies against glutamic acid decarboxylase in newly diagnosed adult-onset diabetes mellitus. Diabetes Research & Clinical Practice, 33(2), 89-97. doi: 10.1016/0168-8227(96)01281-8

Forbes, L. V., Scott, R. S., Brown, L. J., & Darlow, B. A. (1995). Immunogenetic, clinical and demographic characterization of childhood type 1 diabetes in New Zealand. Diabetes Care, 18(11), 1428-1433.

Rudert, F., Visser, E., Forbes, L., Lindridge, E., Wang, L., & Watson, J. (1995). Identification of a silencer, enhancer, and basal promoter region in the human CD95 (Fas/APO-1) gene. DNA & Cell Biology, 14(11), 931-937.

Rudert, F., Roos, M., Forbes, L., & Watson, J. (1994). Apoptosis in L929 cells expressing a CD40/Fas chimeric receptor: Dissociation of stimulatory from inhibitory death signalling functions. Biochemical & Biophysical Research Communications, 204(3), 1102-1110. doi: 10.1006/bbrc.1994.2576

Forbes, L. V., Brown, L. J., & Scott, R. S. (1993). HLA-DQβ typing and non-Asp57 alleles in IDDM and nondiabetic subjects in New Zealand. Diabetes Care, 16(8), 1179-1183.

Schmidli, R. S., Hagan, C., Scott, R. S., Livesey, J., & Forbes, L. V. (1993). Plasma proinsulin in recently diagnosed type 2 diabetes mellitus. Diabetes Research & Clinical Practice, 20(2), 133-138. doi: 10.1016/0168-8227(93)90007-R

Scott, R. S., Brown, L. J., Darlow, B. A., Forbes, L. V., & Moore, M. P. (1992). Temporal variation in incidence of IDDM in Canterbury, New Zealand. Diabetes Care, 15(7), 895-899. doi: 10.2337/diacare.15.7.895

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Journal - Research Other

Carr, A., Pullar, J., Forbes, L., & Rosengrave, P. (2014). Working mothers are disadvantaged by limited funding for health research. New Zealand Medical Journal, 127(1398). Retrieved from http://www.nzma.org.nz/journal

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