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Piet Laboratory

Research interests

Current research in the lab at the Centre for Neuroendocrinology focuses on understanding the neurophysiological mechanisms involved in regulating the activity of the gonadotropin releasing hormone (GnRH) neurons and, ultimately, fertility in all mammals.

We are particularly interested in the afferent control of GnRH neurons by kisspeptin neurons in the preoptic area and in their regulation by circulating gonadal steroid hormones and by the circadian clock.

Clinical conditions

  • Infertility

Dr Richard Piet's researcher profile

List of publications for Richard Piet (Google Scholar)

Piet Laboratory staff & students

Bradley Jamieson_webBradley Jamieson, PhD Student
Role of suprachiasmatic vasopressin neurons in the control of RP3V kisspeptin neurons
Email jambr243@student.otago.ac.nz

Simone Thomas_webSimone Thomas, Research assistant
Projections of suprachiasmatic nucleus neurons to the preoptic area
Email simone.thomas@otago.ac.nz

Yeri Rim, Honours student
Neuromedin S neurons and GnRH neurons

Visit Our people for an alphabetical list of all Centre for Neuroendocrinology staff.

Publications

Piet, R., Kalil, B., McLennan, T., Porteous, R., Czieselsky, K., & Herbison, A. E. (2018). Dominant neuropeptide co-transmission in kisspeptin-GABA regulation of GnRH neuron firing driving ovulation. Journal of Neuroscience, 0658-18, 0658-18. doi: 10.1523/jneurosci.0658-18.2018

Clarkson, J., Han, S. Y., Piet, R., McLennan, T., Kane, G. M., Ng, J., Porteous, R. W., Kim, J. S., … Iremonger, K. J., & Herbison, A. E. (2017). Definition of the hypothalamic GnRH pulse generator in mice. PNAS, 114(47), E10216-E10223. doi: 10.1073/pnas.1713897114

Piet, R., Dunckley, H., Lee, K., & Herbison, A. E. (2016). Vasoactive intestinal peptide excites GnRH neurons in male and female mice. Endocrinology, 157(9), 3621-3630. doi: 10.1210/en.2016-1399

Piet, R., Fraissenon, A., Boehm, U., & Herbison, A. E. (2015). Estrogen permits vasopressin signaling in preoptic kisspeptin neurons in the female mouse. Journal of Neuroscience, 35(17), 6881-6892. doi: 10.1523/jneurosci.4587-14.2015

Piet, R., Boehm, U., & Herbison, A. E. (2013). Estrous cycle plasticity in the hyperpolarization-activated current Ih is mediated by circulating 17β-estradiol in preoptic area kisspeptin neurons. Journal of Neuroscience, 33(26), 10828-10839. doi: 10.1523/JNEUROSCI.1021-13.2013

Chapter in Book - Research

Piet, R., & Herbison, A. E. (2018). Electrophysiology of rodent GnRH neurons. In A. E. Herbison & T. M. Plant (Eds.), The GnRHS neuron and its control. (pp. 177-202). Hoboken, NJ: Wiley Blackwell. doi: 10.1002/9781119233275

Oliet, S. H. R., Panatier, A., Piet, R., Mothet, J.-P., Poulain, D. A., & Theodosis, D. T. (2008). Neuron-glia interactions in the rat supraoptic nucleus. In I. D. Neumann & R. Landgraf (Eds.), Advances in vasopressin and oxytocin: From genes to behaviour to disease: Progress in brain research (Vol. 170). (pp. 109-117). Elsevier. doi: 10.1016/S0079-6123(08)00410-X

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Journal - Research Article

Schafer, D., Kane, G., Colledge, W. H., Piet, R., & Herbison, A. E. (2018). Sex- and sub region-dependent modulation of arcuate kisspeptin neurons by vasopressin and vasoactive intestinal peptide. Journal of Neuroendocrinology. Advance online publication. doi: 10.1111/jne.12660

Han, S. Y., Clarkson, J., Piet, R., & Herbison, A. E. (2018). Optical approaches for interrogating neural circuits controlling hormone secretion [Review]. Endocrinology. Advance online publication. doi: 10.1210/en.2018-00594

Hellier, V., Brock, O., Candlish, M., Desroziers, E., Aoki, M., Mayer, C., Piet, R., Herbison, A., … Bakker, J. (2018). Female sexual behavior in mice is controlled by kisspeptin neurons. Nature Communications, 9, 400. doi: 10.1038/s41467-017-02797-2

Piet, R., Kalil, B., McLennan, T., Porteous, R., Czieselsky, K., & Herbison, A. E. (2018). Dominant neuropeptide co-transmission in kisspeptin-GABA regulation of GnRH neuron firing driving ovulation. Journal of Neuroscience, 0658-18, 0658-18. doi: 10.1523/jneurosci.0658-18.2018

Clarkson, J., Han, S. Y., Piet, R., McLennan, T., Kane, G. M., Ng, J., Porteous, R. W., Kim, J. S., … Iremonger, K. J., & Herbison, A. E. (2017). Definition of the hypothalamic GnRH pulse generator in mice. PNAS, 114(47), E10216-E10223. doi: 10.1073/pnas.1713897114

Manfredi-Lozano, M., Roa, J., Ruiz-Pino, F., Piet, R., García-Galiano, D., Pineda, R., … Herbison, A. E., … Tena-Sempere, M. (2016). Defining a novel leptin-melanocortin-kisspeptin pathway involved in the metabolic control of puberty. Molecular Metabolism, 5(10), 844-857. doi: 10.1016/j.molmet.2016.08.003

Piet, R., Dunckley, H., Lee, K., & Herbison, A. E. (2016). Vasoactive intestinal peptide excites GnRH neurons in male and female mice. Endocrinology, 157(9), 3621-3630. doi: 10.1210/en.2016-1399

Piet, R., Fraissenon, A., Boehm, U., & Herbison, A. E. (2015). Estrogen permits vasopressin signaling in preoptic kisspeptin neurons in the female mouse. Journal of Neuroscience, 35(17), 6881-6892. doi: 10.1523/jneurosci.4587-14.2015

Piet, R., de Croft, S., Liu, X., & Herbison, A. E. (2015). Electrical properties of kisspeptin neurons and their regulation of GnRH neurons. Frontiers in Neuroendocrinology, 35, 15-27. doi: 10.1016/j.yfrne.2014.05.006

Piet, R., Boehm, U., & Herbison, A. E. (2013). Estrous cycle plasticity in the hyperpolarization-activated current Ih is mediated by circulating 17β-estradiol in preoptic area kisspeptin neurons. Journal of Neuroscience, 33(26), 10828-10839. doi: 10.1523/JNEUROSCI.1021-13.2013

Constantin, S., Piet, R., Iremonger, K., Yeo, S. H., Clarkson, J., Porteous, R., & Herbison, A. E. (2012). GnRH neuron firing and response to GABA in vitro depend on acute brain slice thickness and orientation. Endocrinology, 153(8), 3758-3769. doi: 10.1210/en.2012-1126

Brown, R. S. E., Piet, R., Herbison, A. E., & Grattan, D. R. (2012). Differential actions of prolactin on electrical activity and intracellular signal transduction in hypothalamic neurons. Endocrinology, 153(5), 2375-2384. doi: 10.1210/en.2011-2005

de Croft, S., Piet, R., Mayer, C., Mai, O., Boehm, U., & Herbison, A. E. (2012). Spontaneous kisspeptin neuron firing in the adult mouse reveals marked sex and brain region differences but no support for a direct role in negative feedback. Endocrinology, 153(11), 5384-5393. doi: 10.1210/en.2012-1616

Piet, R., Garenne, A., Farrugia, F., Le Masson, G., Marsicano, G., Chavis, P., & Manzoni, O. J. (2011). State-dependent, bidirectional modulation of neural network activity by endocannabinoids. Journal of Neuroscience, 31(46), 16591-16596. doi: 10.1523/jneurosci.4297-11.2011

Piet, R., & Jahr, C. E. (2007). Glutamatergic and purinergic receptor-mediated calcium transients in Bergmann glial cells. Journal of Neuroscience, 27(15), 4027-4035. doi: 10.1523/JNEUROSCI.0462-07.2007

Oliet, S. H. R., Baimoukhametova, D. V., Piet, R., & Bains, J. S. (2007). Retrograde regulation of GABA transmission by the tonic release of oxytocin and endocannabinoids governs postsynaptic firing. Journal of Neuroscience, 27(6), 1325-1333. doi: 10.1523/JNEUROSCI.2676-06.2007

Theodosis, D. T., Koksma, J.-J., Trailin, A., Langle, S. L., Piet, R., Lodder, J. C., … Brussaard, A. B. (2006). Oxytocin and estrogen promote rapid formation of functional GABA synapses in the adult supraoptic nucleus. Molecular & Cellular Neuroscience, 31, 785-794. doi: 10.1016/j.mcn.2006.01.006

Oliet, S. H. R., Piet, R., Poulain, D. A., & Theodosis, D. T. (2004). Glial modulation of synaptic transmission: Insights from the supraoptic nucleus of the hypothalamus. GLIA, 47, 258-267. doi: 10.1002/glia.20032

Piet, R., Poulain, D. A., & Oliet, S. H. R. (2004). Contribution of astrocytes to synaptic transmission in the rat supraoptic nucleus. Neurochemistry International, 45, 251-257. doi: 10.1016/j.neuint.2003.07.005

Theodosis, D. T., Piet, R., Poulain, D. A., & Oliet, S. H. R. (2004). Neuronal, glial and synaptic remodeling in the adult hypothalamus: Functional consequences and role of cell surface and extracellular matrix adhesion molecules. Neurochemistry International, 45, 491-501. doi: 10.1016/j.neuint.2003.11.003

Piet, R., Vargová, L., Syková, E., Poulain, D. A., & Oliet, S. H. R. (2004). Physiological contribution of the astrocytic environment of neurons to intersynaptic crosstalk. PNAS, 101(7), 2151-2155. doi: 10.1073/pnas.0308408100

Piet, R., Bonhomme, R., Theodosis, D. T., Poulain, D. A., & Oliet, S. H. R. (2003). Modulation of GABAergic transmission by endogenous glutamate in the rat supraoptic nucleus. European Journal of Neuroscience, 17, 1777-1785. doi: 10.1046/j.1460-9568.2003.02611.x

Azdad, K., Piet, R., Poulain, D. A., & Oliet, S. H. R. (2003). Dopamine D4 receptor-mediated presynaptic inhibition of GABAergic transmission in the rat supraoptic nucleus. Journal of Neurophysiology, 90, 559-565. doi: 10.1152/jn.00226.2003

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