Research interests
Our major research interest at the Centre for Neuroendocrinology is in the neuroendocrinology of stress.
Current research focuses on two major areas. First, understanding the complex regulation of the adrenal medullary chromaffin cell, specifically how it regulates its secretory output of catecholamines and peptides in response to multiple stress-activated inputs. Much recent work has been directed at understanding how cytokines interact with this stress response.
Our second major interest concerns the role of another cytokine, prolactin, on the stress axis. Specifically we are interested in how stressors alter prolactin secretion from the pituitary and what role this prolactin then plays in stress adaptation under different physiological states.
- Neuroendocrinology of stress
- Cell biology of the adrenal medulla
- Prolactin and the stress axis
Clinical conditions
Impaired or inappropriate adaptation to stress contributes to many major disease states.
Associate Professor Bunn's researcher profile
List of publications for Stephen Bunn (Google Scholar)
Bunn Laboratory staff & students
Joe Yip, Postdoctoral Fellow
Remodelling of hypothalamic neurons during pregnancy and lactation
Email joe.yip@otago.ac.nz
Will Aye, Masters student
Heterogenous stress responses of adrenal medullary chromaffin cells
Visit Our people for an alphabetical list of all Centre for Neuroendocrinology staff.
Publications
Yip, S. H., Romanò, N., Gustafson, P., Hodson, D. J., Williams, E. J., Kokay, I. C., … Grattan, D. R., & Bunn, S. J. (2019). Elevated prolactin during pregnancy drives a phenotypic switch in mouse hypothalamic dopaminergic neurons. Cell Reports, 26(7), 1787-1799.e5. doi: 10.1016/j.celrep.2019.01.067
Yip, S. H., York, J., Hyland, B., Bunn, S. J., & Grattan, D. R. (2018). Incomplete concordance of dopamine transporter Cre (DATIREScre)-mediated recombination and tyrosine hydroxylase immunoreactivity in the mouse forebrain. Journal of Chemical Neuroanatomy, 90, 40-48. doi: 10.1016/j.jchemneu.2017.12.002
Bunn, S., Yip, S. H., Grattan, D., Hyland, B., Szawka, R., Araujo-Lopes, R., & York, J. (2018). Morphological plasticity of hypothalamic dopaminergic neurons during estrous cycle and lactation. Proceedings of the 9th International Congress of Neuroendocrinology (ICN). (pp. 123-124). Retrieved from http://www.icn2018.org/
Kirk, S. E., Xie, T. Y., Steyn, F. J., Grattan, D. R., & Bunn, S. J. (2017). Restraint stress increases prolactin-mediated phosphorylation of signal transducer and activator of transcription 5 in the hypothalamus and adrenal cortex in the male mouse. Journal of Neuroendocrinology, 29(6), 1-9. doi: 10.1111/jne.12477
Gustafson, P., Bunn, S. J., & Grattan, D. R. (2017). The role of prolactin in the suppression of Crh mRNA expression during pregnancy and lactation in the mouse. Journal of Neuroendocrinology, 29(9), e12511. doi: 10.1111/jne.12511
Edited Book - Research
Powis, D. A., & Bunn, S. J. (Eds.). (1995). Neurotransmitter Release and its Modulation. Cambridge: Cambridge University Press, 10p.
Journal - Research Article
Yip, S. H., Romanò, N., Gustafson, P., Hodson, D. J., Williams, E. J., Kokay, I. C., … Grattan, D. R., & Bunn, S. J. (2019). Elevated prolactin during pregnancy drives a phenotypic switch in mouse hypothalamic dopaminergic neurons. Cell Reports, 26(7), 1787-1799.e5. doi: 10.1016/j.celrep.2019.01.067
Yip, S. H., York, J., Hyland, B., Bunn, S. J., & Grattan, D. R. (2018). Incomplete concordance of dopamine transporter Cre (DATIREScre)-mediated recombination and tyrosine hydroxylase immunoreactivity in the mouse forebrain. Journal of Chemical Neuroanatomy, 90, 40-48. doi: 10.1016/j.jchemneu.2017.12.002
Kirk, S. E., Xie, T. Y., Steyn, F. J., Grattan, D. R., & Bunn, S. J. (2017). Restraint stress increases prolactin-mediated phosphorylation of signal transducer and activator of transcription 5 in the hypothalamus and adrenal cortex in the male mouse. Journal of Neuroendocrinology, 29(6), 1-9. doi: 10.1111/jne.12477
Gustafson, P., Bunn, S. J., & Grattan, D. R. (2017). The role of prolactin in the suppression of Crh mRNA expression during pregnancy and lactation in the mouse. Journal of Neuroendocrinology, 29(9), e12511. doi: 10.1111/jne.12511
Gustafson, P., Kokay, I., Sapsford, T., Bunn, S., & Grattan, D. (2017). Prolactin regulation of the HPA axis is not mediated by a direct action upon CRH neurons: Evidence from the rat and mouse. Brain Structure & Function, 222(7), 3191-3204. doi: 10.1007/s00429-017-1395-1
Jenkins, D. E., Sreenivasan, D., Carman, F., Babru, S., Eiden, L. E., & Bunn, S. J. (2016). Interleukin-6-mediated signaling in adrenal medullary chromaffin cells. Journal of Neurochemistry, 139(6), 1138-1150. doi: 10.1111/jnc.13870
Romanò, N., Yip, S. H., Hodson, D. J., Guillou, A., Parnaudeau, S., Kirk, S., … Bunn, S. J., Grattan, D. R., … Martin, A. O. (2013). Plasticity of hypothalamic dopamine neurons during lactation results in dissociation of electrical activity and release. Journal of Neuroscience, 33(10), 4424-4433. doi: 10.1523/jneurosci.4415-12.2013
Samal, B., Ait-Ali, D., Bunn, S., Mustafa, T., & Eiden, L. E. (2013). Discrete signal transduction pathway utilization by a neuropeptide (PACAP) and a cytokine (TNF-alpha) first messenger in chromaffin cells, inferred from coupled transcriptome-promoter analysis of regulated gene cohorts. Peptides, 45, 48-60. doi: 10.1016/j.peptides.2013.03.020
Bunn, S. J., Ait-Ali, D., & Eiden, L. E. (2012). Immune-neuroendocrine integration at the adrenal gland: Cytokine control of the adrenomedullary transcriptome. Journal of Molecular Neuroscience, 48(2), 413-419. doi: 10.1007/s12031-012-9745-1
Yip, S. H., Eguchi, R., Grattan, D. R., & Bunn, S. J. (2012). Prolactin signalling in the mouse hypothalamus is primarily mediated by signal transducer and activator of transcription factor 5b but not 5a. Journal of Neuroendocrinology, 24(12), 1484-1491. doi: 10.1111/j.1365-2826.2012.02357.x
Knowles, P. J., Douglas, S. A., & Bunn, S. J. (2011). Nicotinic stimulation of catecholamine synthesis and tyrosine hydroxylase phosphorylation in cervine adrenal medullary chromaffin cells. Journal of Neuroendocrinology, 23(3), 224-231. doi: 10.1111/j.1365-2826.2010.02100.x
Douglas, S. A., Sreenivasan, D., Carman, F. H., & Bunn, S. J. (2010). Cytokine interactions with adrenal medullary chromaffin cells. Cellular & Molecular Neurobiology, 30(8), 1467-1475. doi: 10.1007/s10571-010-9593-x
Douglas, S. A., & Bunn, S. J. (2009). Interferon-α signalling in bovine adrenal chromaffin cells: Involvement of signal-transducer and activator of transcription 1 and 2, extracellular signal-regulated protein kinases 1/2 and serine 31 phosphorylation of tyrosine hydroxylase. Journal of Neuroendocrinology, 21(3), 200-207. doi: 10.1111/j.1365-2826.2009.01821.x
Douglas, S. A., Stevenson, K. E., Knowles, P. J., & Bunn, S. J. (2008). Characterization of catecholamine release from deer adrenal medullary chromaffin cells. Neuroscience Letters, 445(1), 126-129. doi: 10.1016/j.neulet.2008.08.060
Grattan, D. R., Steyn, F. J., Kokay, I. C., Anderson, G. M., & Bunn, S. J. (2008). Pregnancy-induced adaptation in the neuroendocrine control of prolactin secretion. Journal of Neuroendocrinology, 20(4), 497-507. doi: 10.1111/j.1365-2826.2008.01661.x
Anderson, G. M., Beijer, P., Bang, A. S., Fenwick, M. A., Bunn, S. J., & Grattan, D. R. (2006). Suppression of prolactin-induced signal transducer and activator of transcription 5b signaling and induction of suppressors of cytokine signaling messenger ribonucleic acid in the hypothalamic arcuate nucleus of the rat during late pregnancy and lactation. Endocrinology, 147(10), 4996-5005.
O'Sullivan, K. M., & Bunn, S. J. (2006). Phospholipase C isozymes are differentially distributed in the rat adrenal medulla. Neuroscience Letters, 396, 212-216. doi: 10.1016/j.neulet.2005.11.033
Ma, F. Y., Grattan, D. R., Goffin, V., & Bunn, S. J. (2005). Prolacting-regulated tyrosine hydroxylase activity and messenger ribonucleic acid expression in mediobasal hypothalamic cultures: The differential role of specific protein kinases. Endocrinology, 146(1), 93-102.
Ma, F. Y., Anderson, G. M., Gunn, T. D., Goffin, V., Grattan, D. R., & Bunn, S. J. (2005). Prolactin specifically activates signal transducer and activator of transcription 5b in neuroendocrine dopaminergic neurons. Endocrinology, 146(12), 5112-5119.
Ma, F. Y., Grattan, D. R., Bobrovskaya, L., Dunkley, P. R., & Bunn, S. J. (2004). Angiotensin II regulates tyrosine hydroxylase activity and mRNA expression in rat mediobasal hypothalamic cultures: The role of specific protein kinases. Journal of Neurochemistry.
Tompkins, M., Gai, W. P., Douglas, S., & Bunn, S. J. (2003). α-Synuclein expression localizes to the Golgi apparatus in bovine adrenal medullary chromaffin cells. Brain Research, 984, 233-236.
O'Connell, G., Douglas, S., & Bunn, S. J. (2003). The involvement of specific phospholipase C isozymes in catecholamine release from digitonin permeabilized bovine adrenal medullary chromaffin cells. Neuroscience Letters, 342, 1-4.
Grattan, D. R., Xu, J., McLachlan, M. J., Kokay, I. C., Bunn, S. J., Hovey, R. C., & Davey, H. W. (2001). Feedback regulation of PRL secretion is mediated by the transcription factor, signal transducer, and activator of transcription 5b. Endocrinology, 142(9), 3935-3940.
Grattan, D. R., Pi, X. J., Andrews, Z. B., Augustine, R. A., Kokay, I. C., Summerfield, M. R., Todd, B., & Bunn, S. J. (2001). Prolactin receptors in the brain during pregnancy and lactation: Implications for behaviour. Hormones & Behavior, 40, 115-124.
