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Biochemistry seminar: Oluwatobi Eboda, PhD candidate

Characterization of a novel hiPSC-derived neuronal model of CLN12 Batten disease

Batten disease encompasses a heterogenous group of 13 genetically distinct neurodegenerative lysosomal storage disorders. The group shares many clinical phenotypes including progressive intellectual and motility disability, myoclonic epilepsy, blindness, and cerebral atrophy before eventual patient death. My aim was to establish a novel model of CLN12 Batten disease in human neurons for the assessment of pathological hallmarks. I generated a novel line of iPSC derived CLN12 deficient neuronal cultures to provide accurate modeling of human cell biology. Knockdown of CLN12 coincided with increased α-synuclein deposition, inhibited lysosomal trafficking, changes in total neurite length, increased lysosomal cathepsin activity, decreased mitochondrial membrane potential and health and loss of dendritic synapse density. Collectively, these findings establish a human iPSC model of CLN12 deficiency as a viable tool for further research.


Zoom link for Oluwatobi Eboda's Biochemistry seminar

Zoom password: bioc

Date Tuesday, 21 March 2023
Time 12:00pm - 1:00pm
Audience Undergraduate students,Postgraduate students,Staff,Invite only
Event Category Health Sciences
Event Type Seminar
Online and in-person
LocationBiochemistry Seminar Room G.13 (BIG13), Dunedin and online via Zoom
Contact NameDepartment of Biochemistry

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