Annotation and evolution of bacterial ncRNA genes
Abstract: Successful pathogenic bacteria must alter gene expression in response to changing and hostile environments. Bacterial small non- coding RNAs (sRNAs) contribute to adaptability and pathogenicity by forming complex regulatory networks, but many sRNA genes are difficult to identify and study due to low sequence conservation and condition- specific expression. For my PhD I used a homology search pipeline based on profile hidden Markov models to study the conservation and origins of sRNA genes from Salmonella Typhimurium, which shows that horizontal gene transfer is the main driver of sRNA acquisition in Salmonella. This method was then used to select candidate novel sRNAs from the kiwifruit pathogen Psa, which were annotated from multi-condition transcriptome data, and found that an uncharacterised RNA motif is highly prevalent in the Psa genome.
|Date||Tuesday, 17 March 2020|
|Time||12:00pm - 1:00pm|
|Event Category||Health Sciences|
|Location||New Biochemistry Seminar Room G.13|