Allan Mitchell
Electron microscopy uses a beam of accelerated electrons to visualise specimens. This can create high resolution images that reveal the fine structures of cells, microorganisms, molecules, metals, crystals and many other specimens.
Read more about:
Your project
To start a project, please contact a staff member to discuss options, normally this contact will be Allan Mitchell. If appropriate, we will then ask you to register your intention to use the facility so that we have your details.
If you are new to electron microscopy, discussing your plans before you start collecting specimens, especially biological specimens, is strongly advised. The staff will help you with specimen preparation and, where appropriate, train you to use the equipment.
Depending on your needs and the scale of your project, you may choose to use the facility in a fairly independent manner (subject to appropriate training and staff approval), or have the staff do the bulk of your work.
Initial advice and consultation
Contact for initial advice:
Email allan.mitchell@otago.ac.nz
For advice on the latest academic advances contact:
Dr Mihnea Bostina
Director, Electron Microscopy
Email mihnea.bostina@otago.ac.nz
Services and techniques
Our electron microscopy facility accommodates a broad range of techniques.
Electron microscopy staff expertise
Visit Scanning electron microscopy (SEM) techniques to read more about:
- Array Tomography
- Backscattered Electron Imaging
- Cathodoluminescence
- Cryo-SEM
- Electron Backscatter Diffraction Imaging
- Energy-Dispersive X-ray Spectroscopy
- High Resolution SEM
- Secondary Electron Imaging
- Variable Pressure SEM
Visit Transmission electron microscopy (TEM) techniques to read more about:
- Conventional TEM
- Correlative TEM
- Cryo-TEM
- Cryoultramicrotomy
- Electron Tomography
- Freeze-Fracture TEM
- Heating and Cooling TEM
- High Pressure Freezing (HPF)
- Immunocytochemistry
- Metal Shadowing
- Negative Staining
- Scanning Transmission Electron Microscopy (STEM)
- Single Particle Reconstruction
Other services
- Consumables for Electron Microscopy
- Liquid nitrogen supply
Costs
OMNI reserve the right to alter the costs of the service
Costs below are exclusive of LN2 and any other consumables or chemicals used, these will be charged separately.
Item | Unit Measure | Division of Health Science Users | All Other Users |
|---|---|---|---|
| Alto Sputter Coater | Hour | $10.00 | $40.00 |
| Phillips CM100 TEM | Hour | $60.00 | $240.00 |
| Diamond Knife | Per block | $15.00 | $60.00 |
| Helium Leak Tester | Hour | $150.00 | $600.00 |
| JEOL 6700 FEG SEM | Hour | $60.00 | $240.00 |
| JEOL JEM-2200FS Cryo-TEM | Hour | $60.00 | $200.00 |
| Technical Support | Hour | $20.00 | $80.00 |
| Ultramicrotome | Hour | $20.00 | $80.00 |
| Zeiss FEG SEM | Hour | $60.00 | $240.00 |
| AFS1/2 Equipment Charge | Run | $25.00 | $100.00 |
| Critical Point Dryer (CPD) | Run | $20.00 | $80.00 |
| Edwards Coating | Run | $5.00 | $20.00 |
| Embedding Flexible Mould | Run | 3.00 | $12.00 |
| EmiTech Carbon Coating | Run | $5.00 | $20.00 |
| EmiTech Sputter Coater | Run | $25.00 | $100.00 |
| Freeze Fracture | Run | $40.00 | $160.00 |
| High Pressure Freezer (HPF) | Run | $25.00 | $100.00 |
| KF80 | Run | $25.00 | $100.00 |
| Lynx Tissue Processor | Run | $20.00 | $80.00 |
| Pelco Biowave/Microwave Oven | Run | $10.00 | $40.00 |
| Plasma Glow (Any Machine) | Run | $5.00 | $20.00 |
| Q150 Carbon Coating | Run | $5.00 | $20.00 |
| Vitrobot Equipment charge | Run | $25.00 | $100.00 |
Good preparation helps minimise your costs:
- Good planning (knowing what to expect before starting the investigation)
- Knowing your sample (the time taken to find the information you are looking for—often under-estimated)
- The number of samples you prepare at any one time (very important)
- Consider if new questions may emerge as your investigation proceeds
Email allan.mitchell@otago.ac.nz for further advice.
Student Research Award
The OMNI Electron Microscopy Student Research Award is open to applications twice a year. It provides students with up to 15 hours of free microscopy time. Consult with your supervisor and check out your eligibility.
Equipment
Discussing your Electron Microscopy needs with staff is essential prior to registration and training in the use of equipment. Access to booking calendars is given only after users demonstrate competence on the relevant equipment.
Visit Microscope specifications and sample preparation equipment for more information about:
- JEOL 2200FS Cryo-TEM
- JEOL 6700F FE-SEM
- Philips CM100 TEM
- Zeiss Sigma VP FEG SEM
- SEM and TEM sample preparation equipment
For more about our equipment's capabilities visit:
Registration
To begin a new project please read the User Policy and complete a Project Registration form. This includes projects involving transmission electron microscopy (TEM), scanning electron microscopy (SEM), ultramicrotomy sectioning, specimen preparation, or supply of liquid nitrogen.
- Electron Microscopy User Policy
- OMNI Electron Microscopy Project Registration - internal users
- OMNI Electron Microscopy Project Registration - external users
User Group
The User Group consists of members that represent University of Otago departments that use the Electron Microscopy unit and it advises the OMNI Academic Advisory Group on direction and strategy.
Visit About us for information about how our user groups work and how to make contact.
Training and online resources
Next induction session for new users Wednesday 3 October 2018, email allan.mitchell@otago.ac.nz or sharon.lequeux@otago.ac.nz
Technique training resources and support
Email allan.mitchell@otago.ac.nz if you need help or advice.
- Induction sessions for new users please contact:
allan.mitchell@otago.ac.nz, or
sharon.lequeux@otago.ac.nz
- Visit My Scope for advanced instrumentation and techniques (AMMRF website)
Data analysis resources and support
The Electron Microscopy unit has various computer hardware running OS X and Linux for data processing and analysis. These computers are available for registered users. Please discuss your data processing needs with the staff.
Electron microscopy data and archiving provides advice on protecting your data.
