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Flow cytometry

Michelle Wilson thumbnail
Michelle Wilson

Flow cytometry is used to analyse the physical and chemical characteristics of particles or cells carried in a fluid stream as they pass through at least one laser. Fluorescently-labelled cell components are excited by the laser to emit light at varying wavelengths, the emitted light is measured by detectors.

Flow cytometry is a particularly powerful technique because it allows researchers to rapidly, accurately and simply collect data related to many parameters from a heterogeneous population of cells within a liquid sample.

Katie Young thumbnail
Katie Young

Read more about:

Initial advice and consultation

Contact for initial advice and consultation:
Email michelle.wilson@otago.ac.nz (north campus)
Email katie.young@otago.ac.nz (south campus)

For advice on the latest academic advances contact:
Dr Jo Kirman
Academic Leader, Flow Cytometry
Email jo.kirman@otago.ac.nz

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Services and techniques

  • Multi-colour flow cytometry
  • Cell sorting
  • Analysis workstation
  • Training and support resources

The Flow Cytometry facility is available for studies on:

  • Apoptosis
  • Autophagy
  • Bacteria
  • Cell cycle analysis
  • DNA and RNA content
  • Flow FISH
  • Fluorescent proteins
  • FRET
  • Functional analysis
  • Insects
  • Multi-colour analysis
  • NADPH
  • Necrosis
  • Neurons
  • Oncosis
  • Rare event analysis
  • Signal transduction pathways (phosphorylation)
  • Transcription factors
  • Translocalion
  • Viability
  • Virus infection
  • and much more!

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Equipment

Project discussion with staff is essential prior to registration and training in the use of equipment.

All resources are subject to a minimum booking time of 30 minutes.

For advice on costs please contact michelle.wilson@otago.ac.nz

Flow cytometric analysers

  • BD Fortessa
  • BC Gallios
  • BD FACS CantoII

Sorting (also known as FACS - Fluorescence Assisted Cell Sorting)

  • BD FACSAria assisted sorts
  • BD FACSAria unassisted sorts and analysis (this is only available for trained and approved individuals)

FACS analysis

  • Analysis Computer and Software (FlowJo, Spice)

Technical consultation

Assistance with experimental design or analysis is available with Katie Young (south campus) or Michelle Wilson (north campus).

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Registration

Registration for users

View our video to learn how to register and book our equipment:

Introductory training

A new user online training video (bdbiosciences website) provides some introductory training about flow cytometry and using our facilities.

User Group

The User Group consists of members that represent University of Otago departments that use the Flow Cytometry unit and it advises the OMNI Academic Advisory Group on direction and strategy.

Visit About us for information about how our user groups work and how to make contact.

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Training and online resources

Technique training resources and support

Email michelle.wilson@otago.ac.nz or katie.young@otago.ac.nz if you need help or advice.

We recommend you start with a new user online training video (bdbiosciences website)

Michelle recommends further online resources including:

Data analysis resources and support

Societies

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