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Flow cytometry

Michelle Wilson thumbnail
Michelle Wilson

Flow cytometry is used to analyse the physical and chemical characteristics of particles or cells carried in a fluid stream as they pass through at least one laser. Fluorescently-labelled cell components are excited by the laser to emit light at varying wavelengths, the emitted light is measured by detectors.

Flow cytometry is a particularly powerful technique because it allows researchers to rapidly, accurately and simply collect data related to many parameters from a heterogeneous population of cells within a liquid sample.

Oliver Eltherington thumb
Oliver Eltherington

Read more about:

Initial advice and consultation

Contact for initial advice and consultation:
Email (north campus)
Email (south campus)

For advice on the latest academic advances contact:
Associate Professor Jo Kirman
Academic Leader, Flow Cytometry

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Services and techniques

  • Multi-colour flow cytometry
  • Cell sorting
  • Analysis workstation
  • Training and support resources

The Flow Cytometry facility is available for studies on:

  • Apoptosis
  • Autophagy
  • Bacteria
  • Cell cycle analysis
  • DNA and RNA content
  • Flow FISH
  • Fluorescent proteins
  • FRET
  • Functional analysis
  • Insects
  • Multi-colour analysis
  • Necrosis
  • Neurons
  • Oncosis
  • Rare event analysis
  • Signal transduction pathways (phosphorylation)
  • Transcription factors
  • Translocalion
  • Viability
  • Virus infection
  • and much more!

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Project discussion with staff is essential prior to registration and training in the use of equipment.

All resources are subject to a minimum booking time of 30 minutes.

For advice on costs please contact

Flow cytometric analysers

  • BD Fortessa
  • BC Gallios
  • BD FACS CantoII

Sorting (also known as FACS - Fluorescence Assisted Cell Sorting)

  • BD FACSAria assisted sorts
  • BD FACSAria unassisted sorts and analysis (this is only available for trained and approved individuals)

FACS analysis

  • Analysis Computer and Software (FlowJo, Spice)

Technical consultation

Assistance with experimental design or analysis is available with Katie Young (south campus) or Michelle Wilson (north campus).

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Instrument Costs per hour

OMNI reserve the right to alter the costs of the service

Costs below are exclusive of any other consumables or chemicals used, these will be charged separately.

Division of Health Sciences Users
Per Hour
All Other Users
Per Hour

Flow Cytometry Analysis ASSISTED:
BC Gallios, BD Fortessa, BD Cantoll

$120.00 $480.00

Flow Cytometry Analysis UNASSISTED:
BC Gallios, BD Fortessa, BD Cantoll

$60.00 $240.00

Flow Cytometry Sorting ASSISTED:
FACSAria Fusion

$120.00 $480.00

Flow Cytometry Sorting UNASSISTED:
FACSAria Fusion

$60.00 $480.00

Flow Cytometry Data Analysis:
FCF analysis computer and software

$10.00 $40.00
Technical Consultation $60.00 $240.00

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Registration for users

View our video to learn how to register and book our equipment:

Introductory training

Visit BD Biosciences website for introductory training about flow cytometry.

User Group

The User Group consists of members that represent University of Otago departments that use the Flow Cytometry unit and it advises the OMNI Academic Advisory Group on direction and strategy.

Visit About us for information about how our user groups work and how to make contact.

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Training and online resources

Technique training resources and support

Email or if you need help or advice.

We recommend you start with a new user online training video (bdbiosciences website)

Michelle recommends further online resources including:

Data analysis resources and support


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