Flow cytometry is used to analyse the physical and chemical characteristics of particles or cells carried in a fluid stream as they pass through at least one laser. Fluorescently-labelled cell components are excited by the laser to emit light at varying wavelengths, the emitted light is measured by detectors.
Flow cytometry is a particularly powerful technique because it allows researchers to rapidly, accurately and simply collect data related to many parameters from a heterogeneous population of cells within a liquid sample.
Read more about:
- Multi-colour flow cytometry
- Cell sorting
- Analysis workstation
- Training and support resources
The Flow Cytometry facility is available for studies on:
- Cell cycle analysis
- DNA and RNA content
- Flow FISH
- Fluorescent proteins
- Functional analysis
- Multi-colour analysis
- Rare event analysis
- Signal transduction pathways (phosphorylation)
- Transcription factors
- Virus infection
- and much more!
Project discussion with staff is essential prior to registration and training in the use of equipment.
All resources are subject to a minimum booking time of 30 minutes.
For advice on costs please contact firstname.lastname@example.org
Flow cytometric analysers
- BD Fortessa
- BC Gallios
- BD FACS CantoII
Sorting (also known as FACS - Fluorescence Assisted Cell Sorting)
- BD FACSAria assisted sorts
- BD FACSAria unassisted sorts and analysis (this is only available for trained and approved individuals)
- Analysis Computer and Software (FlowJo, Spice)
Assistance with experimental design or analysis is available with Katie Young (south campus) or Michelle Wilson (north campus).
View our video to learn how to register and book our equipment:
Visit BD Biosciences website for introductory training about flow cytometry.
The User Group consists of members that represent University of Otago departments that use the Flow Cytometry unit and it advises the OMNI Academic Advisory Group on direction and strategy.
Visit About us for information about how our user groups work and how to make contact.
Technique training resources and support
We recommend you start with a new user online training video (bdbiosciences website)
Michelle recommends further online resources including:
- BC Gallios laser / detector arrays (PDF 26 KB)
- BD FACSAria laser / detector arrays (PDF 430 KB))
- BD FACSCanto II laser / detector arrays (PDF 36 KB)
- BD Fortessa laser / detector arrays (PDF 45 KB)
- Current protocols in cytometry (Wiley website)
- Design your own panels with Fluorofinder (Fluorofinder website)
- Email email@example.com or phone +64 21 608 414 (BD Life Sciences Applications Specialist) for troubleshooting and assistance with multicolor panel design
- Email firstname.lastname@example.org for troubleshooting and multicolour panel design
- Email email@example.com (FlowJoanna) for a quote for assistance with experimental design, analysis and troubleshooting
- Expert Cytometry (ExCyte website) has webinars on a variety of topics
- Flow cytometry controls, instrument setup, and the determination of positivity (Pub Med website)
- Optimising a multicolour experiment (PDF 46 KB)
- Questions to ask when you are critiquing flow cytometry data (PDF 67 KB)
- Subscribe to Purdue flow cytometry group list to search for answers in their archive or to ask questions
- Webinar series on multiparameter flow cytometry (bdbiosciences website)
Data analysis resources and support
- Cytobank website for cloud-based data organising, analysis and visualisation
- FlowJo website for online tutorials. Help can be sought from:
- Information standards for experimental reporting (PDF of ISAC recommendation 78 KB)