Monday, 14 January 2019
A reminder about our workshop on single-particle cryo-EM and tomography will be held at the University of Otago, 28 Jan-1 Feb 2019.
Cryogenic electron microscopy (cryo-EM) in conjunction with computerised image processing now enables the determination of high-resolution structures of macromolecular assemblies that have resisted X-ray crystallography and other approaches.
The resolution revolution witnessed over the past decade has been fuelled by improved instrumentation, including extremely stable transmission electron microscopes and direct electron detectors with unprecedented sensitivity and resolution, as well as improved algorithms and software technologies that utilize modern heterogeneous computer architectures efficiently. Single-particle analysis can now be used routinely to produce maps of a quality that allows accurate modelling of the atomic structures of proteins. Tomography in conjunction with subtomographic averaging has broken the 1nm resolution barrier.
In this workshop, we will provide in-depth training in image processing of electron micrographs in the areas of single-particle analysis and subtomographic averaging. The training will include a series of lectures covering the principles of image formation, the image processing methods typically applied and the latest technologies. The practical sessions will follow a tutorial for single-particle analysis and one for subtomogram averaging in order to provide the students with a complete overview of the possibilities currently available for structural determination with cryo-EM.
- Steven Ludtke, Baylor College of Medicine, Huston,TX, USA
- Stefan Raunser, Max Planck Institute for Structural Biology, Dortmund, Germany
- Hans Elmlund, Monash University, Melbourne, Australia
- Mihnea Bostina, University of Otago, Otago, New Zealand
- Michael Landsberg, The University of Queensland, Brisbane, Australia
- Dominika Elmlund, Monash University, Melbourne, Australia
Software packages covered in the tutorials:
- EMAN2.2 http://blake.bcm.edu/emanwiki/EMAN2/Eman22Release
- SPHIRE http://sphire.mpg.de/
- SIMPLE3.0 http://simplecryoem.com/
The course is intended for PhD students and researchers that are actively performing cryoEM or would like to start in order to bring their projects to the next level.
Applications should include: a short description of current work, future plans and an explanation of why attendance at the course would be beneficial to the applicant's research projects.
All applicants are encouraged to submit an abstract describing a project they are currently working on. On Day 1 we will hold a mini symposium where selected participants will present their work.