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Department of Pharmacology and Toxicology staff profiles

Dr Nina Giles

PositionResearch Fellow
DepartmentDepartment of Pharmacology and Toxicology
QualificationsBSc(Hons) PhD
Research summaryCancer research and free radicals in biochemistry

Research

The healthy cell needs to maintain an optimal balance of reactive oxygen species (ROS), however in many diseases states including cancer the ROS increase beyond the capability of the cell to remove them with the antioxidants available.

Our group tries to capitalise on this imbalance by introducing novel drugs which can utilise the excess ROS to destroy cancerous cells, leaving healthy cells untouched.

To achieve this we aim to try and understand a complex set of redox-sensitive pathways and reactions within cancerous cells.

Techniques employed include:

  • Synthesis of novel drugs
  • Cell cytotoxicity assays
  • Fluorescence imaging
  • Western blotting
  • Nanostring gene expression sequencing

Publications

Zadehvakili, B., Giles, N. M., Fawcett, J. P., & Giles, G. I. (2016). Data on the catalytic mechanism of thiol peroxidase mimics. Data in Brief, 8, 207-210. doi: 10.1016/j.dib.2016.05.037

Xian, S., Parayath, N. N., Nehoff, H., Giles, N. M., & Greish, K. (2015). The use of styrene maleic acid nanomicelles encapsulating the synthetic cannabinoid analog WIN55,212-2 for the treatment of cancer. Anticancer Research, 35(9), 4707-4712.

Aitken, J. B., Shearer, E. L., Giles, N. M., Lai, B., Vogt, B., Reboucas, J. S., … Giles, G. I. (2013). Intracellular targeting and pharmacological activity of the superoxide dismutase mimics MnTE-2-PyP5+ and MnTnHex-2-PyP5+ regulated by their porphyrin ring substituents. Inorganic Chemistry, 52(8), 4121-4123. doi: 10.1021/ic300700g

Giles, N. M., Kumari, S., Stamm, R., Patel, S., & Giles, G. I. (2012). A hydrogen peroxide electrode assay to measure thiol peroxidase activity for organoselenium and organotellurium drugs. Analytical Biochemistry, 429(2), 103-107. doi: 10.1016/j.ab.2012.07.013

Giles, N. M., Kumari, S., Gang, B. P., Yuen, C. W. W., Billaud, E. M. F., & Giles, G. I. (2012). The molecular design of S-nitrosothiols as photodynamic agents for controlled nitric oxide release. Chemical Biology & Drug Design, 80(3), 471-478. doi: 10.1111/j.1747-0285.2012.01420.x

Journal - Research Article

Zadehvakili, B., Giles, N. M., Fawcett, J. P., & Giles, G. I. (2016). Data on the catalytic mechanism of thiol peroxidase mimics. Data in Brief, 8, 207-210. doi: 10.1016/j.dib.2016.05.037

Xian, S., Parayath, N. N., Nehoff, H., Giles, N. M., & Greish, K. (2015). The use of styrene maleic acid nanomicelles encapsulating the synthetic cannabinoid analog WIN55,212-2 for the treatment of cancer. Anticancer Research, 35(9), 4707-4712.

Aitken, J. B., Shearer, E. L., Giles, N. M., Lai, B., Vogt, B., Reboucas, J. S., … Giles, G. I. (2013). Intracellular targeting and pharmacological activity of the superoxide dismutase mimics MnTE-2-PyP5+ and MnTnHex-2-PyP5+ regulated by their porphyrin ring substituents. Inorganic Chemistry, 52(8), 4121-4123. doi: 10.1021/ic300700g

Giles, N. M., Kumari, S., Stamm, R., Patel, S., & Giles, G. I. (2012). A hydrogen peroxide electrode assay to measure thiol peroxidase activity for organoselenium and organotellurium drugs. Analytical Biochemistry, 429(2), 103-107. doi: 10.1016/j.ab.2012.07.013

Giles, N. M., Kumari, S., Gang, B. P., Yuen, C. W. W., Billaud, E. M. F., & Giles, G. I. (2012). The molecular design of S-nitrosothiols as photodynamic agents for controlled nitric oxide release. Chemical Biology & Drug Design, 80(3), 471-478. doi: 10.1111/j.1747-0285.2012.01420.x

Oh, J. Y., Giles, N., Landar, A., & Darley-Usmar, V. (2008). Accumulation of 15-deoxy-Δ12,14-prostaglandin J2 adduct formation with Keap1 over time: Effects on potency for intracellular antioxidant defense induction. Biochemical Journal, 411(2), 297-306. doi: 10.1042/bj20071189

Landar, A., Oh, J.-Y., Giles, N. M., Isom, A., Kirk, M., Barnes, S., & Darley-Usmar, V. M. (2006). A sensitive method for the quantitative measurement of protein thiol modification in response to oxidative stress. Free Radical Biology & Medicine, 40(3), 459-468. doi: 10.1016/j.freeradbiomed.2005.08.046

Landar, A., Giles, N., Zmijewski, J. W., Watanabe, N., Oh, J.-Y., & Darley-Usmar, V. M. (2006). Modification of lipids by reactive oxygen and nitrogen species: The oxy-nitroxy-lipidome and its role in redox cell signaling. Future Lipidology, 1(2), 203-211. doi: 10.2217/17460875.1.2.203

Fry, F. H., Holme, A. L., Giles, N. M., Giles, G. I., Collins, C., Holt, K., … Jacob, C. (2005). Multifunctional redox catalysts as selective enhancers of oxidative stress. Organic & Biomolecular Chemistry, 3(14), 2579-2587. doi: 10.1039/b502197a

Beers, S. A., Buckland, A. G., Giles, N., Gelb, M. H., & Wilton, D. C. (2003). Effect of tryptophan insertions on the properties of the human group IIA phospholipase A2: Mutagenesis produces an enzyme with characteristics similar to those of the human group V phospholipase A2. Biochemistry, 42, 7326-7338. doi: 10.1021/bi0343222

Giles, N. M., Giles, G. I., Holley, J. E., Gutowski, N. J., & Jacob, C. (2003). Targeting oxidative stress-related diseases: Organochalcogen catalysts as redox sensitizers. Biochemical Pharmacology, 66(10), 2021-2028. doi: 10.1016/S0006-2952(03)00544-6

Giles, N. M., Watts, A. B., Giles, G. I., Fry, F. H., Littlechild, J. A., & Jacob, C. (2003). Metal and redox modulation of cysteine protein function. Chemistry & Biology, 10(8), 677-693. doi: 10.1016/S1074-5521(03)00174-1

Giles, N. M., Gutowski, N. J., Giles, G. I., & Jacob, C. (2003). Redox catalysts as sensitisers towards oxidative stress. FEBS Letters, 535, 179-182. doi: 10.1016/S0014-5793(02)03890-5

Jacob, C., Giles, G. I., Giles, N. M., & Sies, H. (2003). Sulfur and selenium: The role of oxidation state in protein structure and function. Angewandte Chemie International Edition, 42(39), 4742-4758. doi: 10.1002/anie.200300573

Giles, N. M., Giles, G. I., & Jacob, C. (2003). Multiple roles of cysteine in biocatalysis. Biochemical & Biophysical Research Communications, (1), 1-4. doi: 10.1016/S0006-291X(02)02770-5

Giles, G. I., Tasker, K. M., Collins, C., Giles, N. M., O'Rourke, E., & Jacob, C. (2002). Reactive sulphur species: An in vitro investigation of the oxidation properties of disulphide S-oxides. Biochemical Journal, 364, 579-585.

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Journal - Research Other

Giles, G. I., Giles, N. M., Collins, C. A., Holt, K., Fry, F. H., Lowden, P. A. S., … Jacob, C. (2003). Electrochemical, in vitro and cell culture analysis of integrated redox catalysts: Implications for cancer therapy. Chemical Communications, 2030-2031. doi: 10.1039/b302877a

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