Accessibility Skip to Global Navigation Skip to Local Navigation Skip to Content Skip to Search Skip to Site Map Menu

Department of Pharmacology and Toxicology staff profiles

Dr Greg Giles

PositionSenior Lecturer
DepartmentDepartment of Pharmacology and Toxicology
QualificationsBSc(Hons) PhD
Research summaryBiological reduction and oxidation

Research

Medicinal Chemistry, Drug Design, Free Radical Biology, Nitric Oxide, Antioxidants, Cancer, Stroke, Heart Disease, Mental Dementia.

Research: The Giles Group is investigating biological reduction and oxidation (REDOX) reactions, a wide-ranging field that encompasses energy metabolism, signal transduction pathways, enzyme function and the chemical defence mechanism of the body's immune system. Each cell in the body has to sustain a reducing environment for normal physiological function. This is achieved by maintaining an antioxidant defence network which utilises enzymes, vitamins and cellular reductants. If this defence is breached then oxidative damage can occur throughout the cell, resulting in impaired function and ultimately cell death. This event, termed oxidative stress, is implicated in the pathologies of many diseases including cancer, aging, diabetes, cardiovascular dysfunction and dementia. We are actively involved in drug discovery projects which look at new ways to protect cells from oxidative damage and so provide urgently needed therapies for clinical use.

Publications

Alimoradi, H., Barzegar-Fallah, A., Sammut, I. A., Griesh, K., & Giles, G. I. (2019). Encapsulation of tDodSNO generates a photoactivated nitric oxide releasing nanoparticle for localized control of vasodilation and vascular hyperpermeability. Free Radical Biology & Medicine, 130, 297-305. doi: 10.1016/j.freeradbiomed.2018.10.433

van Hilst, Q. V. C., Vasdev, R. A. S., Preston, D., Findlay, J. A., Scottwell, S. Ø., Giles, G. I., Brooks, H. J. L., & Crowley, J. D. (2019). Synthesis, characterisation and antimicrobial studies of some 2,6-bis(1,2,3-triazol-4-yl)pyridine ruthenium(II) “click” complexes. Asian Journal of Organic Chemistry. Advance online publication. doi: 10.1002/ajoc.201900088

Alimoradi, H., Greish, K., Gamble, A. B., & Giles, G. I. (2019). Controlled delivery of nitric oxide for cancer therapy. Pharmaceutical Nanotechnology, 7(4), 279-303. doi: 10.2174/2211738507666190429111306

Vasdev, R. A. S., Gaudin, L. F., Preston, D., Jogy, J. P., Giles, G. I., & Crowley, J. D. (2018). Anticancer activity and cisplatin binding ability of bis-quinoline and bis-isoquinoline derived Pd2L44+ metallosupramolecular cages. Frontiers in Chemistry, 6, 563. doi: 10.3389/fchem.2018.00563

Alimoradi, H., Barzegar-Fallah, A., Sammut, I. A., Griesh, K., & Giles, G. I. (2018). Data characterizing the biophysical and nitric oxide release properties of the tDodSNO - Styrene maleic anhydride nanoparticle SMA-tDodSNO [Data article]. Data in Brief. Advance online publication. doi: 10.1016/j.dib.2018.10.149

Chapter in Book - Research

Alimoradi, H., Matikonda, S. S., Gamble, A. B., Giles, G. I., & Greish, K. (2017). Redox activated polymeric nanoparticles in tumor therapy. In E. Andronescu & A. M. Grumezescu (Eds.), Nanostructures for drug delivery. (pp. 327-354). Amsterdam, Netherlands: Elsevier. doi: 10.1016/B978-0-323-46143-6.00010-5

Giles, G. I. (2009). Redox-controlled transcription factors and gene expression. In C. Jacob & P. G. Winyard (Eds.), Redox signaling and regulation in biology and medicine. (pp. 245-270). Weinheim, Germany: Wiley-VCH.

Teng, X., Isbell, T. S., Crawford, J. H., Bosworth, C. A., Giles, G. I., Koenitzer, J. R., … Patel, R. P. (2008). Novel method for measuring S-nitrosothiols using hydrogen sulfide. In Methods in enzymology (Volo. 441: Nitric oxide, part G oxidative and nitrasative stress in redox regulation of cell signaling). (pp. 161-172). San Diego, CA: Academic Press. doi: 10.1016/s0076-6879(08)01209-3

^ Top of page

Journal - Research Article

Alimoradi, H., Barzegar-Fallah, A., Sammut, I. A., Griesh, K., & Giles, G. I. (2019). Encapsulation of tDodSNO generates a photoactivated nitric oxide releasing nanoparticle for localized control of vasodilation and vascular hyperpermeability. Free Radical Biology & Medicine, 130, 297-305. doi: 10.1016/j.freeradbiomed.2018.10.433

van Hilst, Q. V. C., Vasdev, R. A. S., Preston, D., Findlay, J. A., Scottwell, S. Ø., Giles, G. I., Brooks, H. J. L., & Crowley, J. D. (2019). Synthesis, characterisation and antimicrobial studies of some 2,6-bis(1,2,3-triazol-4-yl)pyridine ruthenium(II) “click” complexes. Asian Journal of Organic Chemistry. Advance online publication. doi: 10.1002/ajoc.201900088

Vasdev, R. A. S., Gaudin, L. F., Preston, D., Jogy, J. P., Giles, G. I., & Crowley, J. D. (2018). Anticancer activity and cisplatin binding ability of bis-quinoline and bis-isoquinoline derived Pd2L44+ metallosupramolecular cages. Frontiers in Chemistry, 6, 563. doi: 10.3389/fchem.2018.00563

Giles, G. I., Nasim, M. J., Ali, W., & Jacob, C. (2017). The Reactive Sulfur Species concept: 15 Years On. Antioxidants, 6(2), 38. doi: 10.3390/antiox6020038

Zadehvakili, B., McNeill, S. M., Fawcett, J. P., & Giles, G. I. (2016). The design of redox active thiol peroxidase mimics: Dihydrolipoic acid recognition correlates with cytotoxicity and prooxidant action. Biochemical Pharmacology, 104, 19-28. doi: 10.1016/j.bcp.2016.01.012

Alimoradi, H., Matikonda, S. S., Gamble, A. B., Giles, G. I., & Greish, K. (2016). Hypoxia responsive drug delivery systems in tumor therapy. Current Pharmaceutical Design, 22(19), 2808-2820.

McKay, A. P., Lo, W. K. C., Preston, D., Giles, G. I., Crowley, J. D., Barnsley, J. E., Gordon, K. C., & McMorran, D. A. (2016). Palladium(II) and platinum(II) complexes of ((2-pyridyl)pyrazol-1-ylmethyl)benzoic acids: Synthesis, solid state characterisation and biological cytotoxicity. Inorganica Chimica Acta, 446, 41-53. doi: 10.1016/j.ica.2016.02.038

Preston, D., McNeill, S. M., Lewis, J. E. M., Giles, G. I., & Crowley, J. D. (2016). Enhanced kinetic stability of [Pd2L4] 4+ cages through ligand substitution. Dalton Transactions, 45, 8050-8060. doi: 10.1039/c6dt00133e

Zadehvakili, B., Giles, N. M., Fawcett, J. P., & Giles, G. I. (2016). Data on the catalytic mechanism of thiol peroxidase mimics. Data in Brief, 8, 207-210. doi: 10.1016/j.dib.2016.05.037

McNeill, S. M., Preston, D., Lewis, J. E. M., Robert, A., Knerr-Rupp, K., Graham, D. O., Wright, J. R., Giles, G. I., & Crowley, J. D. (2015). Biologically active [Pd2L4]4+ quadruply-stranded helicates: Stability and cytotoxicity. Dalton Transactions, 44, 11129-11136. doi: 10.1039/c5dt01259g

Lo, W. K. C., Huff, G. S., Preston, D., McMorran, D. A., Giles, G. I., Gordon, K. C., & Crowley, J. D. (2015). A dinuclear platinum(II) N4Py complex: An unexpected coordination mode for N4Py. Inorganic Chemistry, 54(14), 6671-6673. doi: 10.1021/acs.inorgchem.5b01032

Kumari, S., Sammut, I. A., & Giles, G. I. (2014). The design of nitric oxide donor drugs: S-nitrosothiol tDodSNO is a superior photoactivated donor in comparison to GSNO and SNAP. European Journal of Pharmacology, 737, 168-176. doi: 10.1016/j.ejphar.2014.05.012

Aitken, J. B., Shearer, E. L., Giles, N. M., Lai, B., Vogt, B., Reboucas, J. S., … Giles, G. I. (2013). Intracellular targeting and pharmacological activity of the superoxide dismutase mimics MnTE-2-PyP5+ and MnTnHex-2-PyP5+ regulated by their porphyrin ring substituents. Inorganic Chemistry, 52(8), 4121-4123. doi: 10.1021/ic300700g

Ang, A. D., Konigstorfer, A., Giles, G. I., & Bhatia, M. (2012). Measuring free tissue sulfide. Advances in Biological Chemistry, 2(4), 360-365. doi: 10.4236/abc.2012.24044

Giles, N. M., Kumari, S., Stamm, R., Patel, S., & Giles, G. I. (2012). A hydrogen peroxide electrode assay to measure thiol peroxidase activity for organoselenium and organotellurium drugs. Analytical Biochemistry, 429(2), 103-107. doi: 10.1016/j.ab.2012.07.013

Giles, N. M., Kumari, S., Gang, B. P., Yuen, C. W. W., Billaud, E. M. F., & Giles, G. I. (2012). The molecular design of S-nitrosothiols as photodynamic agents for controlled nitric oxide release. Chemical Biology & Drug Design, 80(3), 471-478. doi: 10.1111/j.1747-0285.2012.01420.x

Zadehvakili, B., Fawcett, J. P., & Giles, G. I. (2012). Synthesis, structure activity relationship studies and antioxidant activity evaluation of some organochalcogen catalysts as novel GPx mimics. Free Radical Biology & Medicine, 53(Suppl. 1), S99. doi: 10.1016/j.freeradbiomed.2012.08.206

Aitken, J. B., Lay, P. A., Duong, T. T. H., Aran, R., Witting, P. K., Harris, H. H., … Giles, G. I. (2012). Synchrotron radiation induced X-ray emission studies of the antioxidant mechanism of the organoselenium drug ebselen. Journal of Biological Inorganic Chemistry, 17(4), 589-598. doi: 10.1007/s00775-012-0879-y

Stadler, N., Heeneman, S., Vöö, S., Stanley, N., Giles, G. I., Gang, B. P., … Davies, M. J. (2012). Reduced metal ion concentrations in atherosclerotic plaques from subjects with Type 2 diabetes mellitus. Atherosclerosis, 222(2), 512-518. doi: 10.1016/j.atherosclerosis.2012.03.015

Singh, A., Guidry, L., Narasimhulu, K. V., Mai, D., Trombley, J., Redding, K. E., Giles, G. I., … Steyn, A. J. C. (2007). Mycobacterium tuberculosis WhiB3 responds to O2 and nitric oxide via its [4Fe-4S] cluster and is essential for nutrient starvation survival. PNAS, 104(28), 11562-11567. doi: 10.1073/pnas.0700490104

Giles, G. I. (2006). The redox regulation of thiol dependent signaling pathways in cancer. Current Pharmaceutical Design, 12, 4427-4443.

Kelley, E. E., Hock, T., Khoo, N. K. H., Richardson, G. R., Johnson, K. K., Powell, P. C., Giles, G. I., … Tarpey, M. M. (2006). Moderate hypoxia induces xanthine oxidoreductase activity in arterial endothelial cells. Free Radical Biology & Medicine, 40(6), 952-959. doi: 10.1016/j.freeradbiomed.2005.11.008

Griguer, C. E., Oliva, C. R., Kelley, E. E., Giles, G. I., Lancaster, Jr, J. R., & Gillespie, G. Y. (2006). Xanthine oxidase-dependent regulation of hypoxia-inducible factor in cancer cells. Cancer Research, 66(4), 2257-2263. doi: 10.1158/0008-5472.CAN-05-3364

Fry, F. H., Holme, A. L., Giles, N. M., Giles, G. I., Collins, C., Holt, K., … Jacob, C. (2005). Multifunctional redox catalysts as selective enhancers of oxidative stress. Organic & Biomolecular Chemistry, 3(14), 2579-2587. doi: 10.1039/b502197a

Giles, N. M., Giles, G. I., & Jacob, C. (2003). Multiple roles of cysteine in biocatalysis. Biochemical & Biophysical Research Communications, (1), 1-4. doi: 10.1016/S0006-291X(02)02770-5

Giles, N. M., Gutowski, N. J., Giles, G. I., & Jacob, C. (2003). Redox catalysts as sensitisers towards oxidative stress. FEBS Letters, 535, 179-182. doi: 10.1016/S0014-5793(02)03890-5

Jacob, C., Giles, G. I., Giles, N. M., & Sies, H. (2003). Sulfur and selenium: The role of oxidation state in protein structure and function. Angewandte Chemie International Edition, 42(39), 4742-4758. doi: 10.1002/anie.200300573

Giles, N. M., Giles, G. I., Holley, J. E., Gutowski, N. J., & Jacob, C. (2003). Targeting oxidative stress-related diseases: Organochalcogen catalysts as redox sensitizers. Biochemical Pharmacology, 66(10), 2021-2028. doi: 10.1016/S0006-2952(03)00544-6

Giles, N. M., Watts, A. B., Giles, G. I., Fry, F. H., Littlechild, J. A., & Jacob, C. (2003). Metal and redox modulation of cysteine protein function. Chemistry & Biology, 10(8), 677-693. doi: 10.1016/S1074-5521(03)00174-1

Giles, G. I., Tasker, K. M., Collins, C., Giles, N. M., O'Rourke, E., & Jacob, C. (2002). Reactive sulphur species: An in vitro investigation of the oxidation properties of disulphide S-oxides. Biochemical Journal, 364, 579-585.

^ Top of page

Journal - Research Other

Alimoradi, H., Greish, K., Gamble, A. B., & Giles, G. I. (2019). Controlled delivery of nitric oxide for cancer therapy. Pharmaceutical Nanotechnology, 7(4), 279-303. doi: 10.2174/2211738507666190429111306

Alimoradi, H., Barzegar-Fallah, A., Sammut, I. A., Griesh, K., & Giles, G. I. (2018). Data characterizing the biophysical and nitric oxide release properties of the tDodSNO - Styrene maleic anhydride nanoparticle SMA-tDodSNO [Data article]. Data in Brief. Advance online publication. doi: 10.1016/j.dib.2018.10.149

Giles, G. I., Giles, N. M., Collins, C. A., Holt, K., Fry, F. H., Lowden, P. A. S., … Jacob, C. (2003). Electrochemical, in vitro and cell culture analysis of integrated redox catalysts: Implications for cancer therapy. Chemical Communications, 2030-2031. doi: 10.1039/b302877a

More publications...