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Fronius Lab

Lab personnel

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Research interests

Research in my lab focuses on the function and regulation of ion channels with particular emphasis on:

  • Impact of mechanical forces (shear stress and pressure)
  • Fluid homeostasis in the lung
  • Auto-/paracrine signalling via non-neuronal acetylcholine

Clinical conditions and keywords

Hypertension, cardiovascular disease, pulmonary oedema, cystic fibrosis, pain/touch sensation, epithelial Na+ channel (ENaC), CFTR, mechanotransduction

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Expertise

Technical expertise of the lab:

  • Electrophysiology (patch-clamp, Ussing chamber, microelectrode recordings)
  • Pressure myography
  • Electron microscopy

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Projects

  • Identifying the mechanism how ENaC senses shear stress
  • Characterising the role of vascular ENaC for blood pressure regulation in health and disease
  • The role of the extracellular matrix for shear stress sensation and ENaC regulation

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Current funding

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Teaching

  • HUBS 191 Human Body Systems 1
  • PHSL 233 Cellular, Gastrointestinal and Renal Physiology
  • PHSL 345 Physiological Aspects of Health and Disease
  • PHSL 472 Neurophysiology
  • 400-level Convener in Physiology

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Selected publications

Barth, D., & Fronius, M. (2019). Shear force modulates the activity of acid-sensing ion channels at low pH or in the presence of non-proton ligands. Scientific Reports, 9, 6781. doi: 10.1038/s41598-019-43097-7

Ashley, Z., Mugloo, S., McDonald, F. J., & Fronius, M. (2018). Epithelial Na+ channel differentially contributes to shear stress-mediated vascular responsiveness in carotid and mesenteric arteries from mice. American Journal of Physiology: Heart & Circulatory Physiology, 314(5), H1022-H1032. doi: 10.1152/ajpheart.00506.2017

Saw, E. L., Kakinuma, Y., Fronius, M., & Katare, R. (2018). The non-neuronal cholinergic system in the heart: A comprehensive review. Journal of Molecular & Cellular Cardiology. Advance online publication. doi: 10.1016/j.yjmcc.2018.10.013

Ashley, Z., Barth, D., Baldin, J. P., & Fronius, M. (2018). N-Glycan and heparan sulphate components of the extracellular matrix are essential for laminar shear stress response of the epithelial sodium channel (ENaC). FASEB Journal, 32(1, Suppl.), (pp. 868.1). doi: 10.1096/fasebj.2018.32.1_supplement.868.1

Knoepp, F., Ashley, Z., & Fronius, M. (2018). Arterial shear stress sensation and blood pressure regulation relies on glycosylated asparagines of the epithelial Na+ channel. Heart, Lung & Circulation, 27(Suppl. 2), (pp. S64-S65). doi: 10.1016/j.hlc.2018.06.047

Saw, E., Rawal, S., Bunton, R., Galvin, I., Davis, P., Fronius, M., & Katare, R. (2018). Non-neuronal cholinergic system in the diabetic heart. Heart, Lung & Circulation, 27(Suppl. 2), (pp. S115). doi: 10.1016/j.hlc.2018.06.161

Fronius, M., Ashley, Z., Baldin, J.-P., Barth, D., & Knöpp, F. (2018, December). The extracellular matrix facilitates mechanical activation of epithelial Na+ channel in response to shear force to regulate blood pressure. Verbal presentation at the 41st Scientific Meeting of the Matrix Biology Society of Australia and New Zealand, Auckland, New Zealand.

Knoepp, F., Bettmer, J., & Fronius, M. (2017). Gadolinium released by the linear gadolinium-based contrast-agent Gd-DTPA decreases the activity of human epithelial Na+ channels (ENaCs). Biochimica et Biophysica Acta: Biomembranes, 1859(5), 1040-1048. doi: 10.1016/j.bbamem.2017.02.019

Barth, D., & Fronius, M. (2017). Glycosylated asparagines are important constituents for the shear force dependent activation of the epithelial sodium channel. New Zealand Medical Journal, 130(1460), (pp. 97). Retrieved from http://www.nzma.org.nz/journal

Richter, K., Mathes, V., Fronius, M., Althaus, M., Hecker, A., Krasteva-Christ, G., … Grau, V. (2017). Phosphocholine, a novel unconventional agonist of nicotinic receptors containing subunit α9. Journal of Neurochemistry, 142(Suppl. 2), (pp. 218). doi: 10.1111/jnc.13925

Saw, E. E. L., Rawal, S., Davis, P., Bunton, D., Galvin, I. F., Fronius, M., & Katare, R. (2017, September). Downregulation of non-neuronal cholinergic system (NNCS) reduces glucose transporter in the diabetic heart. Poster session presented at the Medical Sciences Congress (MedSci), Queenstown, New Zealand.

Mugloo, S., Ashley, Z., Leader, C., Bahn, A., Sammut, I. A., Walker, R., McDonald, F. J., & Fronius, M. (2017, September). Hypertension is associated with increased ENaC expression in the arteries of Cyp1a1-Ren2 rats. Verbal presentation at the Medical Sciences Congress (MedSci), Queenstown, New Zealand.

Bussey, C. T., Erickson, J. R., Fronius, M., & Ashley, Z. (2017, September). The role of vascular ENaC in impaired flow-mediated responses of carotid arteries in diabetes. Verbal presentation at the Medical Sciences Congress (MedSci), Queenstown, New Zealand.

Knoepp, F., Ashley, Z., Barth, D., Kazantseva, M., Szczesniak, P. P., Clauss, W. G., … Fronius, M. (2017). Mechanical activation of epithelial Na+ channel relies on an interdependent activity of the extracellular matrix and extracellular N-glycans of αENaC. bioRxiv. doi: 10.1101/102756

Richter, K., Mathes, V., Fronius, M., Althaus, M., Hecker, A., Krasteva-Christ, G., … Grau, V. (2016). Phosphocholine: An agonist of metabotropic but not of ionotropic functions of α9-containing nicotinic acetylcholine receptors. Scientific Reports, 6, 28660. doi: 10.1038/srep28660

Ashley, Z., Leader, C., Sammut, I., McDonald, F., Walker, R., & Fronius, M. (2016). The flow detection role of the vascular epithelial sodium channel (ENaC) in an experimental model of hypertension: The novel target for spironolactone therapy? Journal of Hypertension, 34(Suppl. 2), (pp. e103). [Abstract]

Knoepp, F., Ashley, Z., Barth, D., & Fronius, M. (2016). Shear force sensing of the epithelial Na+ channel (ENaC) involves glycosylated asparagines and the extracellular matrix. FEBS Journal, 283(Suppl.1 ), (pp. 50). doi: 10.1111/febs.13805

Barth, D., Knoepp, F., & Fronius, M. (2016). Glycosylated asparagines are important constituents for the shear force dependent activation of the epithelial sodium channel (ENaC). FASEB Journal, 30(Suppl. 1), 1223.7. doi: 10.1096/fj.1530-6860

Ashley, Z., & Fronius, M. (2016). Epithelial Na+ channel (ENAC) differentially contributes to shear stress-mediated regulation of the vascular tone in murine carotid and mesenteric arteries. Acta Physiologica, 217(Suppl. 708), (pp. 46). doi: 10.1111/apha.12712

Baldin, J.-P., & Fronius, M. (2016, August). Shear force activation of the epithelial sodium channel (ENaC): Role of the β and γ subunits. Poster session presented at the Medical Sciences Congress (MedSci), Nelson, New Zealand.

Mugloo, S., Leader, C., Sammut, I. A., Walker, R., McDonald, F. J., & Fronius, M. (2016, August). Characterising ENaC expression in vasculature. Poster session presented at the Medical Sciences Congress (MedSci), Nelson, New Zealand.

Bussey, C., Mugloo, S., Erickson, J., Fronius, M., & Ashley, Z. (2016, December). β-blockers rescue flow-mediated responses of carotid arteries in diabetes through a reduction in vascular ENaC activity? Poster session presented at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Baldin, J.-P., & Fronius, M. (2016, December). Role of the β and γ subunit of the epithelial sodium channel (ENaC) in shear force sensation. Verbal presentation at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Jennings, M., Ashley, Z., Leader, C., McDonald, F., Sammut, I., Walker, R., & Fronius, M. (2016, December). The ultrastructure of mesenteric arteries in a hypertensive rat model. Verbal presentation at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Ashley, Z. A., Knöpp, F., Barth, D., & Fronius, M. (2016, August-September). Epithelial Na+ channel and the endothelial glycocalyx mediate shear stress responsiveness in arteries. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

Working Paper; Discussion Paper; Technical Report

Knoepp, F., Ashley, Z., Barth, D., Kazantseva, M., Szczesniak, P. P., Clauss, W. G., … Fronius, M. (2017). Mechanical activation of epithelial Na+ channel relies on an interdependent activity of the extracellular matrix and extracellular N-glycans of αENaC. bioRxiv. doi: 10.1101/102756

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Chapter in Book - Research

Fronius, M. (2012). Mechanical forces impair alveolar ion transport processes: A putative mechanism contributing to the formation of pulmonary edema. In E. M. Irusen (Ed.), Lung diseases: Selected state of the art reviews. (pp. 561-578). Rijeka, Croatia: InTech.

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Journal - Research Article

Barth, D., & Fronius, M. (2019). Shear force modulates the activity of acid-sensing ion channels at low pH or in the presence of non-proton ligands. Scientific Reports, 9, 6781. doi: 10.1038/s41598-019-43097-7

Ashley, Z., Mugloo, S., McDonald, F. J., & Fronius, M. (2018). Epithelial Na+ channel differentially contributes to shear stress-mediated vascular responsiveness in carotid and mesenteric arteries from mice. American Journal of Physiology: Heart & Circulatory Physiology, 314(5), H1022-H1032. doi: 10.1152/ajpheart.00506.2017

Saw, E. L., Kakinuma, Y., Fronius, M., & Katare, R. (2018). The non-neuronal cholinergic system in the heart: A comprehensive review. Journal of Molecular & Cellular Cardiology. Advance online publication. doi: 10.1016/j.yjmcc.2018.10.013

Knoepp, F., Bettmer, J., & Fronius, M. (2017). Gadolinium released by the linear gadolinium-based contrast-agent Gd-DTPA decreases the activity of human epithelial Na+ channels (ENaCs). Biochimica et Biophysica Acta: Biomembranes, 1859(5), 1040-1048. doi: 10.1016/j.bbamem.2017.02.019

Richter, K., Mathes, V., Fronius, M., Althaus, M., Hecker, A., Krasteva-Christ, G., … Grau, V. (2016). Phosphocholine: An agonist of metabotropic but not of ionotropic functions of α9-containing nicotinic acetylcholine receptors. Scientific Reports, 6, 28660. doi: 10.1038/srep28660

Hecker, A., Küllmar, M., Wilker, S., Richter, K., Zakrzewicz, A., Atanasova, S., … Fronius, M., … Grau, V. (2015). Phosphocholine-modified macromolecules and canonical nicotinic agonists inhibit ATP-induced IL-1β release. Journal of Immunology, 195, 2325-2334. doi: 10.4049/jimmunol.1400974

Vitzthum, C., Clauss, W. G., & Fronius, M. (2015). Mechanosensitive activation of CFTR by increased cell volume and hydrostatic pressure but not shear stress. Biochemica et Biophysica Acta: Biomembranes, 1848(11), 2942-2951. doi: 10.1016/j.bbamem.2015.09.009

Dittrich, N. P., Kummer, W., Clauss, W. G., & Fronius, M. (2015). Luminal acetylcholine does not affect the activity of the CFTR in tracheal epithelia of pigs. International Immunopharmacology, 29(1), 166-172. doi: 10.1016/j.intimp.2015.08.010

Richter, K., Kiefer, K. P., Grzesik, B. A., Clauss, W. G., & Fronius, M. (2014). Hydrostatic pressure activates ATP-sensitive K+ channels in lung epithelium by ATP release through pannexin and connexin hemichannels. FASEB Journal, 28(1), 45-55. doi: 10.1096/fj.13-229252

Schönberger, M., Althaus, M., Fronius, M., Clauss, W., & Trauner, D. (2014). Controlling epithelial sodium channels with light using photoswitchable amilorides. Nature Chemistry, 6(8), 712-719. doi: 10.1038/nchem.2004

Ashry, O., Schnecko, A., Clauss, W. G., & Fronius, M. (2014). Evidence for expression and function of angiotensin II receptor type 1 in pulmonary epithelial cells. Respiratory Physiology & Neurobiology, 195(1), 37-40. doi: 10.1016/j.resp.2014.02.001

Deckmann, K., Filipski, K., Krasteva-Christ, G., Fronius, M., Althaus, M., Rafiq, A., … Kummer, W. (2014). Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes. PNAS, 111(22), 8287-8292. doi: 10.1073/pnas.1402436111

Hollenhorst, M. I., Lips, K. S., Wolff, M., Wess, J., Gerbig, S., Takats, Z., … Fronius, M. (2012). Luminal cholinergic signalling in airway lining fluid: A novel mechanism for activating chloride secretion via Ca 2+-dependent Cl- and K+ channels. British Journal of Pharmacology, 166(4), 1388-1402. doi: 10.1111/j.1476-5381.2012.01883.x

Wesch, D., Althaus, M., Miranda, P., Cruz-Muros, I., Fronius, M., González-Hernández, T., … Giraldez, T. (2012). Differential N termini in epithelial Na+ channel δ-subunit isoforms modulate channel trafficking to the membrane. American Journal of Physiology: Cell Physiology, 302(6), C868-879. doi: 10.1152/ajpcell.00255.2011

Althaus, M., Urness, K. D., Clauss, W. G., Baines, D. L., & Fronius, M. (2012). The gasotransmitter hydrogen sulphide decreases Na+ transport across pulmonary epithelial cells. British Journal of Pharmacology, 166(6), 1946-1963. doi: 10.1111/j.1476-5381.2012.01909.x

Hollenhorst, M. I., Lips, K. S., Weitz, A., Krasteva, G., Kummer, W., & Fronius, M. (2012). Evidence for functional atypical nicotinic receptors that activate K+-dependent Cl- secretion in mouse tracheal epithelium. American Journal of Respiratory Cell & Molecular Biology, 46(1), 106-114. doi: 10.1165/rcmb.2011-0171OC

Krumm, P., Giraldez, T., Alvarez de la Rosa, D., Clauss, W. G., Fronius, M., & Althaus, M. (2012). Thiol-reactive compounds from garlic inhibit the epithelial sodium channel (ENaC). Bioorganic & Medicinal Chemistry, 20(13), 3979-3984. doi: 10.1016/j.bmc.2012.05.021

Althaus, M., Pichl, A., Clauss, W. G., Seeger, W., Fronius, M., & Morty, R. E. (2011). Nitric oxide inhibits highly selective sodium channels and the Na+/K+-ATPase in H441 cells. American Journal of Respiratory Cell & Molecular Biology, 44(1), 53-65. doi: 10.1165/2009-0335OC

Kessler, S., Clauss, W. G., Günther, A., Kummer, W., & Fronius, M. (2011). Expression and functional characterization of P2X receptors in mouse alveolar macrophages. Pflügers Archiv: European Journal of Physiology, 462(3), 419-430. doi: 10.1007/s00424-011-0980-z

Berger, J., Richter, K., Clauss, W. G., & Fronius, M. (2011). Evidence for basolateral Cl- channels as modulators of apical Cl- secretion in pulmonary epithelia of Xenopus laevis. American Journal of Physiology: Regulatory, Integrative & Comparative Physiology, 300(3), R616-R623. doi: 10.1152/ajpregu.00464.2010

Althaus, M., Clauss, W. G., & Fronius, M. (2011). Amiloride-sensitive sodium channels and pulmonary edema. Pulmonary Medicine, 2011, 830320. doi: 10.1155/2011/830320

Hollenhorst, M. I., Richter, K., & Fronius, M. (2011). Ion transport by pulmonary epithelia. Journal of Biomedicine & Biotechnology, 2011, 174306. doi: 10.1155/2011/174306

Kessler, S., Clauss, W. G., & Fronius, M. (2011). Laminar shear stress modulates the activity of heterologously expressed P2X4 receptors. Biochimica et Biophysica Acta: Biomembranes, 1808(10), 2488-2495. doi: 10.1016/j.bbamem.2011.07.010

Mikulski, Z., Hartmann, P., Jositsch, G., Zasłona, Z., Lips, K. S., Pfeil, U., … Fronius, M., & Kummer, W. (2010). Nicotinic receptors on rat alveolar macrophages dampen ATP-induced increase in cytosolic calcium concentration. Respiratory Research, 11, 133. doi: 10.1186/1465-9921-11-133

Berger, J., Hardt, M., Clauss, W. G., & Fronius, M. (2010). Basolateral Cl- uptake mechanisms in Xenopus laevis lung epithelium. American Journal of Physiology: Regulatory, Integrative & Comparative Physiology, 299(1), R92-R100. doi: 10.1152/ajpregu.00749.2009

Fronius, M., Bogdan, R., Althaus, M., Morty, R. E., & Clauss, W. G. (2010). Epithelial Na+ channels derived from human lung are activated by shear force. Respiratory Physiology & Neurobiology, 170(1), 113-119. doi: 10.1016/j.resp.2009.11.004

Althaus, M., Fronius, M., Buchäckert, Y., Vadász, I., Clauss, W. G., Seeger, W., … Morty, R. E. (2009). Carbon monoxide rapidly impairs alveolar fluid clearance by inhibiting epithelial sodium channels. American Journal of Respiratory Cell & Molecular Biology, 41(6), 639-650. doi: 10.1165/rcmb.2008-0458OC

Fronius, M., & Clauss, W. G. (2008). Mechano-sensitivity of ENaC: May the (shear) force be with you. Pflügers Archiv: European Journal of Physiology, 455(5), 775-785. doi: 10.1007/s00424-007-0332-1

Bogdan, R., Veith, C., Clauss, W., & Fronius, M. (2008). Impact of mechanical stress on ion transport in native lung epithelium (Xenopus laevis): Short-term activation of Na+, Cl- and K+ channels. Pflügers Archiv: European Journal of Physiology, 456(6), 1109-1120. doi: 10.1007/s00424-008-0486-5

Althaus, M., Bogdan, R., Clauss, W. G., & Fronius, M. (2007). Mechano-sensitivity of epithelial sodium channels (ENaCs): Laminar shear stress increases ion channel open probability. FASEB Journal, 21(10), 2389-2399. doi: 10.1096/fj.06-7694com

Sommer, D., Bogdan, R., Berger, J., Peters, D. M., Morty, R. E., Clauss, W. G., & Fronius, M. (2007). CFTR-dependent Cl- secretion in Xenopus laevis lung epithelium. Respiratory Physiology & Neurobiology, 158(1), 97-106. doi: 10.1016/j.resp.2007.03.016

Schnizler, M. K., Bogdan, R., Bennert, A., Bury, N. R., Fronius, M., & Clauss, W. (2007). Short-term exposure to waterborne free silver has acute effects on membrane current of Xenopus oocytes. Biochimica et Biophysica Acta: Biomembranes, 1768(2), 317-323. doi: 10.1016/j.bbamem.2006.09.007

Krumm, S., Goebel-Lauth, S. G., Fronius, M., & Clauss, W. (2005). Transport of sodium and chloride across earthworm skin in vitro. Journal of Comparative Physiology B, 175, 601-608. doi: 10.1007/s00360-005-0023-1

Fronius, M., Berk, A., Clauss, W., & Schnizler, M. (2004). Ion transport across Xenopus alveolar epithelium is regulated by extracellular ATP, UTP and adenosine. Respiratory Physiology & Neurobiology, 139, 133-144. doi: 10.1016/j.resp.2003.09.007

Berk, A., Fronius, M., Clauss, W., & Schnizler, M. (2004). Prostaglandin E2 induces upregulation of Na+ transport across Xenopus lung epithelium. Journal of Comparative Physiology B, 174, 83-89. doi: 10.1007/s00360-003-0391-3

Fronius, M., Clauss, W., & Schnizler, M. (2003). Stimulation of transepithelial Na+ current by extracellular Gd3+ in Xenopus laevis alveolar epithelium. Journal of Membrane Biology, 195(1), 43-51. doi: 10.1007/s00232-003-2043-7

Fronius, M., Rehn, M., Eckstein-Ludwig, U., & Clauss, W. (2001). Inhibitory non-genomic effects of progesterone on Na+ absorption in epithelial cells from Xenopus kidney (A6). Journal of Comparative Physiology B, 171, 377-386. doi: 10.1007/s003600100186

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Journal - Research Other

Fronius, M. (2013). Treatment of pulmonary edema by ENaC activators/stimulators. Current Molecular Pharmacology, 6(1), 13-27. doi: 10.2174/1874467211306010003

Fronius, M., Clauss, W. G., & Althaus, M. (2012). Why do we have to move fluid to be able to breathe? Frontiers in Physiology, 3, 146. doi: 10.3389/fphys.2012.00146

Hollenhorst, M. I., Clauss, W., & Fronius, M. (2012). Acetylcholin als non-neuronales Signalmolekül [The non-neuronal cholinergic system]. Biologie in unserer Zeit, 42(6), 390-395. doi: 10.1002/biuz.201210495

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Conference Contribution - Published proceedings: Full paper

Hollenhorst, M. I., Lips, K. S., Kummer, W., & Fronius, M. (2012). Nicotine-induced activation of soluble adenylyl cyclase participates in ion transport regulation in mouse tracheal epithelium. Life Sciences, 91(21-22), (pp. 1009-1012). doi: 10.1016/j.lfs.2012.06.027

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Conference Contribution - Published proceedings: Abstract

Ashley, Z., Barth, D., Baldin, J. P., & Fronius, M. (2018). N-Glycan and heparan sulphate components of the extracellular matrix are essential for laminar shear stress response of the epithelial sodium channel (ENaC). FASEB Journal, 32(1, Suppl.), (pp. 868.1). doi: 10.1096/fasebj.2018.32.1_supplement.868.1

Saw, E., Rawal, S., Bunton, R., Galvin, I., Davis, P., Fronius, M., & Katare, R. (2018). Non-neuronal cholinergic system in the diabetic heart. Heart, Lung & Circulation, 27(Suppl. 2), (pp. S115). doi: 10.1016/j.hlc.2018.06.161

Knoepp, F., Ashley, Z., & Fronius, M. (2018). Arterial shear stress sensation and blood pressure regulation relies on glycosylated asparagines of the epithelial Na+ channel. Heart, Lung & Circulation, 27(Suppl. 2), (pp. S64-S65). doi: 10.1016/j.hlc.2018.06.047

Barth, D., & Fronius, M. (2017). Glycosylated asparagines are important constituents for the shear force dependent activation of the epithelial sodium channel. New Zealand Medical Journal, 130(1460), (pp. 97). Retrieved from http://www.nzma.org.nz/journal

Richter, K., Mathes, V., Fronius, M., Althaus, M., Hecker, A., Krasteva-Christ, G., … Grau, V. (2017). Phosphocholine, a novel unconventional agonist of nicotinic receptors containing subunit α9. Journal of Neurochemistry, 142(Suppl. 2), (pp. 218). doi: 10.1111/jnc.13925

Ashley, Z., Leader, C., Sammut, I., McDonald, F., Walker, R., & Fronius, M. (2016). The flow detection role of the vascular epithelial sodium channel (ENaC) in an experimental model of hypertension: The novel target for spironolactone therapy? Journal of Hypertension, 34(Suppl. 2), (pp. e103). [Abstract]

Ashley, Z., & Fronius, M. (2016). Epithelial Na+ channel (ENAC) differentially contributes to shear stress-mediated regulation of the vascular tone in murine carotid and mesenteric arteries. Acta Physiologica, 217(Suppl. 708), (pp. 46). doi: 10.1111/apha.12712

Barth, D., Knoepp, F., & Fronius, M. (2016). Glycosylated asparagines are important constituents for the shear force dependent activation of the epithelial sodium channel (ENaC). FASEB Journal, 30(Suppl. 1), 1223.7. doi: 10.1096/fj.1530-6860

Knoepp, F., Ashley, Z., Barth, D., & Fronius, M. (2016). Shear force sensing of the epithelial Na+ channel (ENaC) involves glycosylated asparagines and the extracellular matrix. FEBS Journal, 283(Suppl.1 ), (pp. 50). doi: 10.1111/febs.13805

Knoepp, F., Szczesniak, P., Althaus, M., & Fronius, M. (2015). Glycosylated extracellular asparagines of the α-subunit provide a connection to the extracellular matrix and facilitate the shear force-dependent activation of the human epithelial Na+-channel (ENaC). FASEB Journal, 29(1, Suppl.), 845.1. Retrieved from http://www.fasebj.org/content/29/1_Supplement/845.1.abstract

Knoepp, F., Clauss, W., & Fronius, M. (2014). Gd3+ and Gd3+-containing contrast agents affect human ENaC activity in Xenopus ocytes. FASEB Journal, 28(1, Suppl.), 1181.14. [Abstract]

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Conference Contribution - Poster Presentation (not in published proceedings)

Saw, E. E. L., Rawal, S., Davis, P., Bunton, D., Galvin, I. F., Fronius, M., & Katare, R. (2017, September). Downregulation of non-neuronal cholinergic system (NNCS) reduces glucose transporter in the diabetic heart. Poster session presented at the Medical Sciences Congress (MedSci), Queenstown, New Zealand.

Baldin, J.-P., & Fronius, M. (2016, August). Shear force activation of the epithelial sodium channel (ENaC): Role of the β and γ subunits. Poster session presented at the Medical Sciences Congress (MedSci), Nelson, New Zealand.

Bussey, C., Mugloo, S., Erickson, J., Fronius, M., & Ashley, Z. (2016, December). β-blockers rescue flow-mediated responses of carotid arteries in diabetes through a reduction in vascular ENaC activity? Poster session presented at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Mugloo, S., Leader, C., Sammut, I. A., Walker, R., McDonald, F. J., & Fronius, M. (2016, August). Characterising ENaC expression in vasculature. Poster session presented at the Medical Sciences Congress (MedSci), Nelson, New Zealand.

Ashley, Z., & Fronius, M. (2015, August-September). The vascular responses to modulation of flow, but not pressure, are dependent on vascular ENaC function in carotid, femoral and mesenteric arteries from mice. Poster session presented at the Medical Sciences Congress (MedSci), Queenstown, New Zealand.

Barth, D., Knoepp, F., & Fronius, M. (2015, August-September). Mechanotransduction of ENaC and the extracellular matrix. Poster session presented at the Medical Sciences Congress (MedSci), Queenstown, New Zealand.

Fronius, M., Knoepp, F., Bednarz, M., & Althaus, M. (2013, August). The δ1 and δ2 ENaC subunits form mechanosensitive channels when coexpressed with β and γ subunits. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

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Conference Contribution - Verbal presentation and other Conference outputs

Fronius, M., Ashley, Z., Baldin, J.-P., Barth, D., & Knöpp, F. (2018, December). The extracellular matrix facilitates mechanical activation of epithelial Na+ channel in response to shear force to regulate blood pressure. Verbal presentation at the 41st Scientific Meeting of the Matrix Biology Society of Australia and New Zealand, Auckland, New Zealand.

Mugloo, S., Ashley, Z., Leader, C., Bahn, A., Sammut, I. A., Walker, R., McDonald, F. J., & Fronius, M. (2017, September). Hypertension is associated with increased ENaC expression in the arteries of Cyp1a1-Ren2 rats. Verbal presentation at the Medical Sciences Congress (MedSci), Queenstown, New Zealand.

Bussey, C. T., Erickson, J. R., Fronius, M., & Ashley, Z. (2017, September). The role of vascular ENaC in impaired flow-mediated responses of carotid arteries in diabetes. Verbal presentation at the Medical Sciences Congress (MedSci), Queenstown, New Zealand.

Baldin, J.-P., & Fronius, M. (2016, December). Role of the β and γ subunit of the epithelial sodium channel (ENaC) in shear force sensation. Verbal presentation at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Knöpp, F., Ashley, Z., Barth, D., Baldin, J.-P., & Fronius, M. (2016, December). Epithelial Na+ channel and the extracellular matrix: An intimate relationship that facilitates shear force responsiveness. Verbal presentation at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Ashley, Z., Leader, C., Sammut, I., McDonald, F., Walker, R., & Fronius, M. (2016, December). Role of ENaC in the dysfunction of resistance arteries in hypertension with/without the confounding factor of myocardial infarction. Verbal presentation at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Mugloo, S., Ashley, Z., Leader, C., Bahn, A., Sammut, I., Walker, R., McDonald, F., & Fronius, M. (2016, December). Epithelial Sodium Channel (ENaC): An emerging regulator of vascular physiology. Verbal presentation at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Barth, D., & Fronius, M. (2016, December). The epithelial sodium channel is activated by shear force via glycosylated asparagines and the extracellular matrix. Verbal presentation at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Jennings, M., Ashley, Z., Leader, C., McDonald, F., Sammut, I., Walker, R., & Fronius, M. (2016, December). The ultrastructure of mesenteric arteries in a hypertensive rat model. Verbal presentation at the Joint International Meeting of the Renal Scientists of the Australia and New Zealand Society of Nephrology and the Kidney in Health and Disease Network: Translational Medicine: Innovations in Renal Research, Blenheim, New Zealand.

Ashley, Z. A., Knöpp, F., Barth, D., & Fronius, M. (2016, August-September). Epithelial Na+ channel and the endothelial glycocalyx mediate shear stress responsiveness in arteries. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

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