Profile

Contact Details

Email: jithendra.ratnayake@otago.ac.nz

University Links

Department

Department of Oral Sciences

Qualifications

BEng(Hons) (Sheffield) PhD (Otago)

Research summary

Biomaterials and regenerative medicine

Teaching

Oral biology, biomaterials and bioengineering

Memberships

European Society of Biomaterials (ESB)
Australasian Society for Biomaterials and Tissue Engineering (ASBTE)
International Association of Dental Research (IADR)
Materials Research Society (MRS)

Research

My main area of research is focused on developing novel biomaterials from waste resources for biomedical applications.

I became interested in adding value to the waste products from New Zealand’s annual $NZ6 billion red meat export industry. I have significant expertise in characterising biomaterials using various analytical techniques and evaluating biocompatibility using in-vitro and in-vivo techniques.

My secondary research interests lie in developing medical devices, dental materials, restorative dentistry and dental education.

Current research strategies are focused on:

Developing novel bone grafts from bovine and ovine bone for bone regeneration applications
Developing bio-composite materials for biomedical applications (wound dressing, cleft lip palate repair, and orthopaedic applications)
Development and testing of a bovine-derived nanohydroxyapatite toothpaste for caries management
Development of a hybrid composite from natural Montmorillonite clay and waste bovine bone for dental restoration applications
Synthesis of A Triple-functionalized Multilayer on Porous CFR-PEEK Implants

Additional details

I serve as an adjunct Senior Lecturer at the Kothelawala Defence University (KDU), Sri Lanka. I have commercial collaborations with Colgate Palmolive Ltd, ANZCO foods and Intuitive 8 Healthcare LLC (India) and Universities in the UK, Australia, Saudi Arabia, China, Sri Lanka and the USA.

Before moving to New Zealand, I served in the British and Sri Lankan military. Outside of work, you can find me on the cricket field or walking my two dogs.

Publications

Ratnayake, J., Gould, M., Ramesh, N., Mucalo, M., & Dias, G. (2024). A porous fluoride-substituted bovine-derived hydroxyapatite scaffold constructed for applications in Bone Tissue Regeneration. Materials, 17, 1107. doi: 10.3390/ma17051107 Journal - Research Article

Choudhury, M., Brunton, P., Schwass, D., Pletzer, D., Ratnayake, J., Dias, G., & Tompkins, G. (2024). Effectiveness of gold nanoparticles in prevention and treatment of oral mucositis in animal models: A systematic review. Systematic Reviews, 13(1), 39. doi: 10.1186/s13643-023-02425-9 Journal - Research Other

Jum'ah, A., Kothari, S., Gray, A. R., Ratnayake, J., Leov, F., Lyons, K., & Brunton, P. A. (2024). A randomized clinical trial investigating the effect of three vital tooth bleaching protocols on oral health-related quality of life. Saudi Dental Journal, 36(1), 77-83. doi: 10.1016/j.sdentj.2023.09.010 Journal - Research Article

Khurshid, Z., Adanir, N., Ratnayake, J., Dias, G., & Cooper, P. R. (2023). Demineralized dentin matrix for bone regeneration in dentistry: A critical update. Saudi Dental Journal. Advance online publication. doi: 10.1016/j.sdentj.2023.11.028 Journal - Research Other

Lin, J., Bennani, V., Aarts, J. M., Brunton, P., & Ratnayake, J. (2023). Factors influencing success rate of ceramic veneers on endodontically treated anterior teeth: A systematic review. Journal of Prosthetic Dentistry. Advance online publication. doi: 10.1016/j.prosdent.2023.10.031 Journal - Research Other

2024

Journal - Research Article

Journal - Research Other

2023

Chapter in Book - Research

Ratnayake, J., Camilleri, J., Haththotuwa, T. N., & Huang, J. (2023). In vitro biological testing of dental materials. In G. J. Seymour, M. P. Cullinan, N. C. K. Heng & P. R. Cooper (Eds.), Oral biology: Molecular techniques and applications: Methods in molecular biology (Vol. 2588). (3rd ed.) (pp. 505-524). New York, NY: Springer. doi: 10.1007/978-1-0716-2780-8_31

Journal - Research Article

Ajay Sharma, L., Ramesh, N., Sharma, A., Ratnayake, J. T. B., Love, R. M., Alavi, S. E., Wilson, M. J., & Dias, G. J. (2023). In vitro effects of wool-derived keratin on human dental pulp-derived stem cells for endodontic applications. British Journal of Oral & Maxillofacial Surgery. Advance online publication. doi: 10.1016/j.bjoms.2023.08.240

Khurshid, Z., Alfarhan, M. F. A., Bayan, Y., Mazher, J., Adanir, N., Dias, G. J., Cooper, P. R., & Ratnayake, J. (2023). Development, physicochemical characterization and in-vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair. PeerJ. Advance online publication. doi: 10.7717/peerj.15711

Khashashneh, M., Ratnayake, J., Choi, J. J. E., Mei, L., Lyons, K., & Brunton, P. (2023). The effect of 10% carbamide peroxide dental bleaching on the physical properties of Invisalign aligners: An in vitro study. Materials, 16, 4125. doi: 10.3390/ma16114125

Gould, M. L., Ratnayake, J. T. B., Ramesh, N., Powlay, T. J., Curnow, O. J., Staiger, M. P., & Dias, G. J. (2023). In vivo biocompatibility of non-derivatized cellulose regenerated using ionic liquids. Journal of Polymers & the Environment, 31, 1335-1350. doi: 10.1007/s10924-022-02640-w

Ahmed, H., Ratnayake, J., Cathro, P., & Chandler, N. (2023). The effect of an additional application of sealer prior to backfilling in the Continuous Wave of Condensation technique. Australian Endodontic Journal, 49, 344-350. doi: 10.1111/aej.12658

Journal - Research Other

Dohan, Z., Friedlander, L. T., Cooper, P. R., Li, K.-C., Ratnayake, J. T., & Mei, M. L. (2023). In vitro models used in the formation of root caries lesions: A review of the literature. Dentistry Journal, 11, 269. doi: 10.3390/dj11120269

Yang, R., Zhao, R., Chaudry, F., Wang, T., Brunton, P., Khurshid, Z., & Ratnayake, J. (2023). Modern sedative agents and techniques used in dentistry for patients with special needs: A review. Journal of Taibah University Medical Sciences. Advance online publication. doi: 10.1016/j.jtumed.2023.10.004

Talesara, V., Bennani, V., Aarts, J., Ratnayake, J., Khurshid, Z., & Brunton, P. (2023). Accuracy of digitally coded healing abutments: A systematic review. Saudi Dental Journal. Advance online publication. doi: 10.1016/j.sdentj.2023.08.006

Cathro, P., Smith, M., Ratnayake, J., & Heithersay, G. (2023). Micro-CT evaluation of spontaneous apexification of an immature tooth following trauma. Case Reports in Dentistry, 2023, 3779225. doi: 10.1155/2023/3779225

Imran, E., Cooper, P. R., Ratnayake, J., Ekambaram, M., & Mei, M. L. (2023). Potential beneficial effects of hydroxyapatite nanoparticles on caries lesions in vitro: A review of the literature. Dentistry Journal, 11, 40. doi: 10.3390/dj11020040

2022

Journal - Research Article

Khurshid, Z., Alfarhan, M. F., Mazher, J., Bayan, Y., Cooper, P. R., Dias, G. J., … Ratnayake, J. (2022). Extraction of hydroxyapatite from camel bone for bone tissue engineering application. Molecules, 27(22), 7946. doi: 10.3390/molecules27227946

Cai, M., Ratnayake, J., Cathro, P., Gould, M., & Ali, A. (2022). Investigation of a novel injectable chitosan oligosaccharide-bovine hydroxyapatite hybrid dental biocomposite for the purposes of conservative pulp therapy. Nanomaterials, 12, 3925. doi: 10.3390/nano12213925

Mansoor, A., Khurshid, Z., Mansoor, E., Khan, M. T., Ratnayake, J., & Jamal, A. (2022). Effect of currently available nanoparticle synthesis routes on their biocompatibility with fibroblast cell lines. Molecules, 27, 6972. doi: 10.3390/molecules27206972

Ratnayake, J., Veerasamy, A., Ahmed, H., Coburn, D., Loch, C., Gray, A. R., Lyons, K. M., Heng, N. C. K., Cannon, R. D., … Brunton, P. A. (2022). Clinical and microbiological evaluation of a chlorhexidine-modified glass ionomer cement (GIC-CHX) restoration placed using the atraumatic restorative treatment (ART) technique. Materials, 15, 5044. doi: 10.3390/ma15145044

Khurshid, Z., Asiri, F. Y. I., Najeeb, S., & Ratnayake, J. (2022). The impact of autologous platelet concentrates on the periapical tissues and root development of replanted teeth: A systematic review. Materials, 15, 2776. doi: 10.3390/ma15082776

Yong, D., Choi, J. J. E., Cathro, P., Cooper, P. R., Dias, G., Huang, J., & Ratnayake, J. (2022). Development and analysis of a hydroxyapatite supplemented calcium silicate cement for endodontic treatment. Materials, 15(3), 1176. doi: 10.3390/ma15031176

Kumar Yadalam, P., Madapusi Balaji, T., Varadarajan, S., Alzahrani, K. J., Al-Ghamdi, M. S., Ali Baeshen, H., … Ratnayake, J., & Patil, S. (2022). Assessing the therapeutic potential of angomelatine, ramelteon, and melatonin against SARS-Cov-2. Saudi Journal of Biological Sciences, 29, 3140-3150. doi: 10.1016/j.sjbs.2022.01.049

Ratnayake, J., Guan, G., Polonowita, A., Gray, A. R., Loch, C., Li, K. C., Waddell, J. N., Lyons, K., & Brunton, P. A. (2022). Measuring changes in jaw opening forces to assess the degree of improvement in patients with temporomandibular disorders. Applied Sciences, 12(3), 1224. doi: 10.3390/app12031224

Olson, H., Ratnayake, J., Senthilkumar, A., Quaranta, A., & Meldrum, A. (2022). Working characteristics for practicing dental hygienists in two countries in different hemispheres. International Journal of Dental Hygiene, 20, 209-218. doi: 10.1111/idh.12538

Journal - Research Other

Khashashneh, M., Ratnayake, J., Choi, J. J. E., Mei, L., Lyons, K., & Brunton, P. A. (2022). The effectiveness of dental bleaching during orthodontic treatment with clear aligners: A systematic review. Applied Sciences, 12(21), 11274. doi: 10.3390/app122111274

Arora, S., Cooper, P. R., Ratnayake, J. T., Friedlander, L. T., Rizwan, S. B., Seo, B., & Hussaini, H. M. (2022). A critical review of in vitro research methodologies used to study mineralization in human dental pulp cell cultures. International Endodontic Journal, 55(Suppl. 1), 3-13. doi: 10.1111/iej.13684

Journal - Professional & Other Non-Research Articles

Ratnayake, J. (2022). [Review of the book Understanding Platelet-Rich Fibrin]. New Zealand Dental Journal, 118(3), 115. [Book Review].

Ratnayake, J. (2022). [Review of the book Dermal fillers for dental professionals]. New Zealand Dental Journal, 118(2), 76. [Book Review].

Conference Contribution - Published proceedings: Abstract

Choudhury, M., Tompkins, G., Brunton, P., Dias, G., Pletzer, D., & Ratnayake, J. (2022). Gold nanoparticles against oropharyngeal pathogens in cancer therapy. New Zealand Dental Journal, 118(4), (pp. 168). [Abstract]

2021

Chapter in Book - Research

Ramesh, N., Ratnayake, J. T. B., & Dias, G. J. (2021). Calcium-based ceramic biomaterials. In C. Wen (Ed.), Structural biomaterials: Properties, characteristics, and selection. (pp. 333-394). Duxford, UK: Woodhead Publishing. doi: 10.1016/B978-0-12-818831-6.00011-2

Journal - Research Article

Ngah, N. A., Dias, G. J., Tong, D. C., Mohd Noor, S. N. F., Ratnayake, J., Cooper, P. R., & Hussaini, H. M. (2021). Lyophilised platelet-rich fibrin: Physical and biological characterisation. Molecules, 26(23), 7131. doi: 10.3390/molecules26237131

Brunton, P. A., Ratnayake, J., Bodansky, H. J., Mei, L., Veerasamy, A., & Hall, R. (2021). An intraoral device for weight loss: Initial clinical findings. British Dental Journal. Advance online publication. doi: 10.1038/s41415-021-3081-1

Aghoghovwia, B. E., Okpe, O., McIntyre, E. A., Taniyohwo, E. M., & Ratnayake, J. T. (2021). A brief review on erythropoietin and mesenchymal stem cell therapies for paediatric neurological disorders. Current Stem Cell Reports, 7, 95-107. doi: 10.1007/s40778-021-00189-3

Journal - Research Other

Chen, L., Al-Bayatee, S., Khurshid, Z., Shavandi, A., Brunton, P., & Ratnayake, J. (2021). Hydroxyapatite in oral care products: A review. Materials, 14, 4865. doi: 10.3390/ma14174865

Zhao, R., Yang, R., Cooper, P. R., Khurshid, Z., Shavandi, A., & Ratnayake, J. (2021). Bone grafts and substitutes in dentistry: A review of current trends and developments. Molecules, 26(10), 3007. doi: 10.3390/molecules26103007

Oliveira, É. R., Nie, L., Podstawczyk, D., Allahbakhsh, A., Ratnayake, J., Brasil, D. L., & Shavandi, A. (2021). Advances in growth factor delivery for bone tissue engineering. International Journal of Molecular Sciences, 22, 903. doi: 10.3390/ijms22020903

Ngah, N. A., Ratnayake, J., Cooper, P. R., Dias, G. J., Tong, D. C., Mohd Noor, S. N. F., & Hussaini, H. M. (2021). Potential of lyophilized platelet concentrates for craniofacial tissue regenerative therapies. Molecules, 26, 517. doi: 10.3390/molecules26030517

Journal - Professional & Other Non-Research Articles

Ratnayake, J. (2021). [Review of the book The tongue]. New Zealand Dental Journal, 117(3), 158. [Book Review].

2020

Journal - Research Article

Ratnayake, J., Guan, G., Polonowita, A., Li, K. C., Gray, A., Waddell, J. N., Loch, C., & Brunton, P. A. (2020). Can the measurement of jaw-opening forces assist in the diagnosis of temporomandibular disorders? Journal of Oral & Facial Pain & Headache, 34(3), 199-205. doi: 10.11607/ofph.2587

Sarfaraz, S., Shabbir, J., Mudasser, M. A., Khurshid, Z., Al-Quraini, A. A. A., Abbasi, M. S., Ratnayake, J., & Zafar, M. S. (2020). Knowledge and attitude of dental practitioners related to disinfection during the COVID-19 pandemic. Healthcare, 8(3), 232. doi: 10.3390/healthcare8030232

Huang, J., Ratnayake, J., Ramesh, N., & Dias, G. J. (2020). Development and characterization of a biocomposite material from chitosan and New Zealand-sourced bovine-derived hydroxyapatite for bone regeneration. ACS Omega, 5, 16537-16546. doi: 10.1021/acsomega.0c01168

Ramesh, N., Ratnayake, J. T. B., Moratti, S. C., & Dias, G. J. (2020). Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration. International Journal of Biological Macromolecules, 160, 1009-1020. doi: 10.1016/j.ijbiomac.2020.05.269

Ratnayake, J. T., Ross, E. D., Dias, G. J., Shanafelt, K. M., Taylor, S. S., Gould, M. L., Guan, G., & Cathro, P. R. (2020). Preparation, characterisation and in-vitro biocompatibility study of a bone graft developed from waste bovine teeth for bone regeneration. Materials Today Communications, 22, 100732. doi: 10.1016/j.mtcomm.2019.100732

Journal - Research Other

Feroz, S., Muhammad, N., Ratnayake, J., & Dias, G. (2020). Keratin-based materials for biomedical applications. Bioactive Materials, 5(3), 496-509. doi: 10.1016/j.bioactmat.2020.04.007

2019

Journal - Research Article

Lu, E. M.-C., Ratnayake, J., & Rich, A. M. (2019). Assessment of proliferating cell nuclear antigen (PCNA) expression at the invading front of oral squamous cell carcinoma. BMC Oral Health, 19, 233. doi: 10.1186/s12903-019-0928-9

Smith, A., Scott, I., Ratnayake, J., Newsham-West, K., & Cathro, P. (2019). An intervention study: Does a cognitive reappraisal technique reduce the perceived stress in fourth-year dental students in New Zealand? International Journal of Dentistry, 2019, 5864591. doi: 10.1155/2019/5864591

Loch, C., Ratnayake, J., Veerasamy, A., Cathro, P., Lee, R., & Brunton, P. A. (2019). Direct restorations, endodontics, and bleaching: Materials and techniques used by general dentists of New Zealand. International Journal of Dentistry, 2019, 6327171. doi: 10.1155/2019/6327171

Brunton, P. A., Ratnayake, J., Loch, C., Veerasamy, A., Cathro, P., & Lee, R. (2019). Indirect restorations and fixed prosthodontics: Materials and techniques used by general dentists of New Zealand. International Journal of Dentistry, 2019, 5210162. doi: 10.1155/2019/5210162

Race, J., Zilm, P., Ratnayake, J., Fitzsimmons, T., Marchant, C., & Cathro, P. (2019). Efficacy of laser and ultrasonic-activated irrigation on eradicating a mixed-species biofilm in human mesial roots. Australian Endodontic Journal, 45, 317-324. doi: 10.1111/aej.12334

Conference Contribution - Published proceedings: Abstract

Ratnayake, J., Guan, S., Polonowita, A., Li, K. C., Waddell, N., Loch, C., Gray, A., & Brunton, P. (2019). Can the measurement of jaw-opening forces assist in diagnosing temporomandibular-disorders? Journal of Dental Research, 98(A), 0179. Retrieved from https://iadr.abstractarchives.com

Ratnayake, J., Ross, E., Dias, G., Guan, S., & Cathro, P. (2019). Development and characterisation of a novel bone-graft from bovine teeth. Journal of Dental Research, 98(A), 0490. Retrieved from https://iadr.abstractarchives.com

Cathro, P., Ratnayake, J., Dias, G., Ross, E., Shanafelt, K., Taylor, S., & Guan, S. (2019). Biocompatibility and bioactivity of a novel bone graft material developed from the dentine of waste bovine teeth. International Endodontic Journal, 52(Suppl. 1), (pp. 12). doi: 10.1111/iej.13172

Ramesh, N., Ratnayake, J., Moratti, S. C., & Dias, G. J. (2019). Effect of chitosan infiltration on the mechanical properties of a porous xenograft material derived from New Zealand sourced bovine cancellous bone. Proceedings of the European Society for Biomaterials (ESB) 30th Annual Conference and the German Society for Biomaterials (DGBM) 26th Annual Conference. PS2-12-441. Retrieved from https://www.esb2019.org/

2018

Journal - Research Article

Stenhouse, M., Zilm, P., Ratnayake, J., & Cathro, P. (2018). Investigation of the effect of rapid and slow external pH increases on Enterococcus faecalis biofilm grown on dentine. Australian Dental Journal, 63(2), 224-230. doi: 10.1111/adj.12582

Lee, R. J., Ratnayake, J., Veerasamy, A., Loch, C., Cathro, P., & Brunton, P. A. (2018). Demographics, practising arrangements, and standards: Survey among New Zealand dentists. International Journal of Dentistry, 2018, 7675917. doi: 10.1155/2018/7675917

Wang, S., Cathro, P., Heithersay, G., Briggs, N., Ratnayake, J., & Zilm, P. (2018). A colorimetric evaluation of the effect of bacterial contamination on teeth stained with blood in vitro: Evaluation of the efficacy of two different bleaching regimes. Australian Dental Journal, 63(2), 253-260. doi: 10.1111/adj.12604

Plutzer, B., Zilm, P., Ratnayake, J., & Cathro, P. (2018). Comparative efficacy of endodontic medicaments & sodium hypochlorite against Enterococcus faecalis biofilms. Australian Dental Journal, 63(2), 208-216. doi: 10.1111/adj.12580

Conference Contribution - Published proceedings: Abstract

Race, J., Zilm, P., Ratnayake, J., & Cathro, P. (2018). Laser and ultrasonic activated irrigation on eradicating a mixed-species biofilm. Journal of Dental Research, 97(Spec. Iss. B), 1322. Retrieved from http://www.iadr.org

Ahmed, H., Ratnayake, J., Loch, C., Lyons, K., & Brunton, P. (2018). Clinical evaluation of modified-GIC restorations: Six month results. Journal of Dental Research, 97(Spec. Iss. B), 1334. Retrieved from http://www.iadr.org

Ratnayake, J., Gould, M., & Dias, G. (2018). Ionic substituted bovine-derived hydroxyapatite bone grafts for dental surgery applications. New Zealand Dental Journal, 114(4), (pp. 183). [Abstract]

Conference Contribution - Verbal presentation and other Conference outputs

Ratnayake, J. (2018, April). Development of a novel bone graft material from New Zealand sourced bovine bone. Verbal presentation at the University of Otago Centre for Bioengineering & Nanomedicine Symposium: Biomaterials and Regenerative Medicine, Dunedin, New Zealand.

2017

Journal - Research Article

Ratnayake, J. T. B., Mucalo, M., & Dias, G. J. (2017). Substituted hydroxyapatites for bone regeneration: A review of current trends. Journal of Biomedical Materials Research Part B, 105(5), 1285-1299. doi: 10.1002/jbm.b.33651

Ratnayake, J. T. B., Gould, M. L., Shavandi, A., Mucalo, M., & Dias, G. J. (2017). Development and characterization of a xenograft material from New Zealand sourced bovine cancellous bone. Journal of Biomedical Materials Research Part B, 105(5), 1054-1062. doi: 10.1002/jbm.b.33644

Conference Contribution - Published proceedings: Abstract

Ratnayake, J., Mucalo, M., & Dias, G. (2017). Development of a xenograft from bovine cancellous bone. Proceedings of the University of Otago Student Research Symposium: Te Wānaka Rakahau: Ākoka. (pp. 74-75). Retrieved from http://www.otago.ac.nz/graduate-research/scholarships/otago643219.html

2015

Journal - Research Article

Shavandi, A., Bekhit, A. E.-D. A., Ali, A., Sun, Z., & Ratnayake, J. T. (2015). Microwave-assisted synthesis of high purity β-tricalcium phosphate crystalline powder from the waste of Green mussel shells (Perna canaliculus). Powder Technology, 273, 33-39. doi: 10.1016/j.powtec.2014.12.029