Dr Julia Horsfield

Julia Horsfiled and the Zebrafish tanksDirector
BSc (Hons), PhD, DipAppSci

Dr Julia Horsfield is a developmental geneticist based in the Department of Pathology at the University of Otago.

Julia leads a research group investigating the role of chromosome structure in animal development and cancer. Her specific research interest lies in cohesin proteins, which are present in all animals and important for cell division and DNA repair. As the name implies, cohesin proteins’ main function is to hold replicated chromosomes together until cells divide. However, they are also involved in gene expression and this is where Julia’s research focus lies, particularly in relation to Cornelia de Lange and Roberts syndromes.

Julia has worked with zebrafish as her model animal for the past 12 years. She moved from the University of Auckland to the University of Otago in 2007, introducing Otago researchers to zebrafish in the process. Her enthusiasm for the zebrafish animal model has attracted several senior researchers and their teams to zebrafish and this has ultimately led to the development of the new state-of-the-art facility.

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Publications

Leask, M., Dowdle, A., Salvesen, H., Topless, R., Fadason, T., Wei, W., … Marsman, J., … Merriman, T. R., & Horsfield, J. A. (2019). Functional urate-associated genetic variants influence expression of lincRNAs LINC01229 and MAFTRR. Frontiers in Genetics, 9, 733. doi: 10.3389/fgene.2018.00733

Kline, A. D., Krantz, I. D., Bando, M., Shirahige, K., Chea, S., Sakata, T., … Horsfield, J. A., … Haaland, R. E. (2019). Cornelia de Lange syndrome, related disorders, and the Cohesin complex: Abstracts from the 8th Biennial Scientific and Educational Symposium 2018 [Conference report]. American Journal of Medical Genetics Part A. Advance online publication. doi: 10.1002/ajmg.a.61108

Meier, M., Grant, J., Dowdle, A. E., Thomas, A., Gerton, J., Collas, P., … Horsfield, J. A. (2019). A zebrafish model for cohesin function in early embryo development. American Journal of Medical Genetics Part A, Advance online publication. doi: 10.1002/ajmg.a.61108

Mehta, S. Y., Morten, B. C., Antony, J., Henderson, L., Lasham, A., Campbell, H., Cunliffe, H., Horsfield, J. A., … Braithwaite, A. W. (2018). Regulation of the interferon-gamma (IFN-γ) pathway by p63 and Δ133p53 isoform in different breast cancer subtypes. Oncotarget, 9(49), 29146-29161. doi: 10.18632/oncotarget.25635

Cukrov, D., Newman, T., Leask, M., Leeke, B., Sarogni, P., Patimo, A., … Horsfield, J., & Musio, A. (2018). Antioxidant treatment ameliorates phenotypic features of SMC1A-mutated Cornelia de Lange syndrome in vitro and in vivo. Human Molecular Genetics. Advance online publication. doi: 10.1093/hmg/ddy203

Journal - Research Article

Leask, M., Dowdle, A., Salvesen, H., Topless, R., Fadason, T., Wei, W., … Marsman, J., … Merriman, T. R., & Horsfield, J. A. (2019). Functional urate-associated genetic variants influence expression of lincRNAs LINC01229 and MAFTRR. Frontiers in Genetics, 9, 733. doi: 10.3389/fgene.2018.00733

Mehta, S. Y., Morten, B. C., Antony, J., Henderson, L., Lasham, A., Campbell, H., Cunliffe, H., Horsfield, J. A., … Braithwaite, A. W. (2018). Regulation of the interferon-gamma (IFN-γ) pathway by p63 and Δ133p53 isoform in different breast cancer subtypes. Oncotarget, 9(49), 29146-29161. doi: 10.18632/oncotarget.25635

Cukrov, D., Newman, T., Leask, M., Leeke, B., Sarogni, P., Patimo, A., … Horsfield, J., & Musio, A. (2018). Antioxidant treatment ameliorates phenotypic features of SMC1A-mutated Cornelia de Lange syndrome in vitro and in vivo. Human Molecular Genetics. Advance online publication. doi: 10.1093/hmg/ddy203

Meier, M., Grant, J., Dowdle, A., Thomas, A., Gerton, J., Collas, P., … Horsfield, J. A. (2018). Cohesin facilitates zygotic genome activation in zebrafish. Development, 145, dev156521. doi: 10.1242/dev.156521

Ketharnathan, S., Leask, M., Boocock, J., Phipps-Green, A. J., Antony, J., O'Sullivan, J. M., Merriman, T. R., & Horsfield, J. A. (2018). A non-coding genetic variant maximally associated with serum urate levels is functionally linked to HNF4A-dependent PDZK1 expression. Human Molecular Genetics, ddy295. Advance online publication. doi: 10.1093/hmg/ddy295

Kaur, G., Li, C. G., Chantry, A., Stayner, C., Horsfield, J., & Eccles, M. R. (2018). SMAD proteins directly suppress PAX2 transcription downstream of transforming growth factor-beta 1 (TGF-β1) signalling in renal cell carcinoma. Oncotarget, 9(42), 26852-26867. doi: 10.18632/oncotarget.25516

Lee, S. L. J., Horsfield, J. A., Black, M. A., Rutherford, K., & Gemmell, N. J. (2018). Identification of sex differences in zebrafish (Danio rerio) brains during early sexual differentiation and masculinisation using 17α-methyltestoterone. Biology of Reproduction, 99(2), 446-460. doi: 10.1093/biolre/iox175

Schierding, W., Antony, J., Karhunen, V., Vääräsmäki, M., Franks, S., Elliott, P., … Horsfield, J. A., … Cutfield, W. S. (2018). GWAS on prolonged gestation (post-term birth): Analysis of successive Finnish birth cohorts. Journal of Medical Genetics, 55(1), 55-63. doi: 10.1136/jmedgenet-2017-104880

Lee, S. L. J., Horsfield, J. A., Black, M. A., Rutherford, K., Fisher, A., & Gemmell, N. J. (2017). Histological and transcriptomic effects of 17α-methyltestosterone on zebrafish gonad development. BMC Genomics, 18, 557. doi: 10.1186/s12864-017-3915-z

McKinlay, P., Keller, C., & Horsfield, J. (2017). It’s written in your DNA: Or is it? Junctures, 2017(18), 81-87.

Marsman, J., Thomas, A., Osato, M., O'Sullivan, J. M., & Horsfield, J. A. (2017). A DNA contact map for the mouse Runx1 gene identifies novel haematopoietic enhancers. Scientific Reports, 7, 13347. doi: 10.1038/s41598-017-13748-8

Stayner, C., Poole, C. A., McGlashan, S. R., Pilanthananond, M., Brauning, R., Markie, D., … Slobbe, L., … Parker, K., Wiles, A., … Leichter, A., Leask, M., … Jennings, M., Horsfield, J. A., Walker, R. J., & Eccles, M. R. (2017). An ovine hepatorenal fibrocystic model of a Meckel-like syndrome associated with dysmorphic primary cilia and TMEM67 mutations. Scientific Reports, 7, 1601. doi: 10.1038/s41598-017-01519-4

McKinlay, P., Pickery, J.-J., & Horsfield, J. (2017). The GENE-ration game. Junctures, 2017(18), 71-80.

Newman, T., Jhinku, N., Meier, M., & Horsfield, J. (2016). Dietary intake influences adult fertility and offspring fitness in zebrafish. PLoS ONE, 11(11), e01663394. doi: 10.1371/journal.pone.0166394

Dasgupta, T., Antony, J., Braithwaite, A. W., & Horsfield, J. A. (2016). HDAC8 inhibition blocks SMC3 deacetylation and delays cell cycle progression without affecting cohesin-dependent transcription in MCF7 cancer cells. Journal of Biological Chemistry, 291(24), 12761-12770. doi: 10.1074/jbc.M115.704627

Schierding, W., Antony, J., Cutfield, W. S., Horsfield, J. A., & O'Sullivan, J. M. (2016). Intergenic GWAS SNPs are key components of the spatial and regulatory network for human growth. Human Molecular Genetics, 25(15), 3372-3382. doi: 10.1093/hmg/ddw165

Chatterjee, A., Lagisz, M., Rodger, E. J., Zhen, L., Stockwell, P. A., Duncan, E. J., Horsfield, J. A., … Nakagawa, S. (2016). Sex differences in DNA methylation and expression in zebrafish brain: A test of an extended ‘male sex drive’ hypothesis. Gene, 590, 307-316. doi: 10.1016/j.gene.2016.05.042

Antony, J., Dasgupta, T., Rhodes, J. M., McEwan, M. V., Print, C. G., O'Sullivan, J. M., & Horsfield, J. A. (2015). Cohesin modulates transcription of estrogen-responsive genes. Biochimica et Biophysica Acta: Gene Regulatory Mechanisms, 1849(3), 257-269. doi: 10.1016/j.bbagrm.2014.12.011

Schuster, K., Leeke, B., Meier, M., Wang, Y., Newman, T., Burgess, S., & Horsfield, J. A. (2015). A neural crest origin for cohesinopathy heart defects. Human Molecular Genetics, 24(24), 7005-7016. doi: 10.1093/hmg/ddv402

Newman, T. A. C., Carleton, C. R., Leeke, B., Hampton, M. B., & Horsfield, J. A. (2015). Embryonic oxidative stress results in reproductive impairment for adult zebrafish. Redox Biology, 6, 648-655. doi: 10.1016/j.redox.2015.10.010

Leeke, B., Marsman, J., O'Sullivan, J. M., & Horsfield, J. A. (2014). Cohesin mutations in myeloid malignancies: Underlying mechanisms. Experimental Hematology & Oncology, 3, 13. doi: 10.1186/2162-3619-3-13

O'Sullivan, J. M., Doynova, M. D., Antony, J., Pichlmuller, F., & Horsfield, J. A. (2014). Insights from space: Potential role of diet in the spatial organization of chromosomes. Nutrients, 6(12), 5724-5739. doi: 10.3390/nu6125724

Marsman, J., O'Neill, A. C., Kao, B. R.-Y., Rhodes, J. M., Meier, M., Antony, J., Mönnich, M., & Horsfield, J. A. (2014). Cohesin and CTCF differentially regulate spatiotemporal runx1 expression during zebrafish development. Biochimica et Biophysica Acta: Gene Regulatory Mechanisms, 1839(1), 50-61. doi: 10.1016/j.bbagrm.2013.11.007

Chatterjee, A., Ozaki, Y., Stockwell, P. A., Horsfield, J. A., Morison, I. M., & Nakagawa, S. (2013). Mapping the zebrafish brain methylome using reduced representation bisulfite sequencing. Epigenetics, 8(9), 979-989. doi: 10.4161/epi.25797

Deardorff, M. A., Wilde, J. J., Albrecht, M., Dickinson, E., Tennstedt, S., Braunholz, D., Mönnich, M., … Horsfield, J. A., & Kaiser, F. J. (2012). RAD21 mutations cause a human cohesinopathy. American Journal of Human Genetics, 90(6), 1014-1027. doi: 10.1016/j.ajhg.2012.04.019

Marsman, J., & Horsfield, J. A. (2012). Long distance relationships: Enhancer-promoter communication and dynamic gene transcription. Biochimica et Biophysica Acta: Gene Regulatory Mechanisms, 1819(11-12), 1217-1227. doi: 10.1016/j.bbagrm.2012.10.008

Horsfield, J. A., Print, C. G., & Mönnich, M. (2012). Diverse developmental disorders from The One Ring: Distinct molecular pathways underlie the cohesinopathies. Frontiers in Genetics, 3, 171. doi: 10.3389/fgene.2012.00171

McEwan, M. V., Eccles, M. R., & Horsfield, J. A. (2012). Cohesin is required for activation of MYC by estradiol. PLoS ONE, 7(11), e49160. doi: 10.1371/journal.pone.0049160

Mönnich, M., Kuriger, Z., Print, C. G., & Horsfield, J. A. (2011). A zebrafish model of Roberts syndrome reveals that Esco2 depletion interferes with development by disrupting the cell cycle. PLoS ONE, 6(5), e20051. doi: 10.1371/journal.pone.0020051

Rhodes, J. M., McEwan, M., & Horsfield, J. A. (2011). Gene regulation by cohesin in cancer: Is the ring an unexpected party to proliferation? Molecular Cancer Research, 9(12), 1587-1607. doi: 10.1158/1541-7786.mcr-11-0382

Rhodes, J. M., Bentley, F. K., Print, C. G., Dorsett, D., Misulovin, Z., Dickinson, E. J., … Horsfield, J. A. (2010). Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved. Developmental Biology, 344, 637-649. doi: 10.1016/j.ydbio.2010.05.493

Mönnich, M., Banks, S., Eccles, M., Dickinson, E., & Horsfield, J. (2009). Expression of cohesin and condensin genes during zebrafish development supports a non-proliferative role for cohesin. Gene Expression Patterns, 9(8), 586-594. doi: 10.1016/j.gep.2009.08.004

Horsfield, J. A., Anagnostou, S. H., Hu, J. K.-H., Cho, K. H. Y., Geisler, R., Lieschke, G., … Crosier, P. S. (2007). Cohesin-dependent regulation of Runx genes. Development, 134, 2639-2649. doi: 10.1242/dev.002485

Brumby, A., Secombe, J., Horsfield, J., Coombe, M., Amin, N., Coates, D., … Richardson, H. (2004). A genetic screen for dominant modifiers of a cyclin E hypomorphic mutation identifies novel regulators of S-phase entry in drosophilia. Genetics, 168(1), 227-251. doi: 10.1534/genetics.104.026617

Kalev-Zylinska, M. L., Horsfield, J. A., Flores, M. V., Postlethwait, J. H., Chau, J. Y., Cattin, P. M., … Crosier, K. E. (2003). Runx3 is required for hematopoietic development in zebrafish. Developmental Dynamics, 228(3), 323-336. doi: 10.1002/dvdy.10388

Crosier, P. S., Kalev-Zylinska, M. L., Hall, C. J., Flores, M. V. C., Horsfield, J. A., & Crosier, K. E. (2002). Pathways in blood and vessel development revealed through zebrafish genetics. International Journal of Developmental Biology, 46, 493-502.

Horsfield, J., Ramachandran, A., Reuter, K., Lavallie, E., Collins-Racie, L., Crosier, K., & Crosier, P. (2002). Cadherin-17 is required to maintain pronephric duct integrity during zebrafish development. Mechanisms of Development, 115, 15-26. doi: 10.1016/S0925-4773(02)00094-1

Brumby, A. M., Zraly, C. B., Horsfield, J. A., Secombe, J., Saint, R., Dingwall, A. K., & Richardson, H. (2002). Drosophila cyclin E interacts with components of the Brahma complex. EMBO Journal, 21(13), 3377-3389. doi: 10.1093/emboj/cdf334

Irvine, J., Horsfield, J. A., McKinney, C. Z., & Tate, W. P. (1998). A novel strategy to interfere with human immunodeficiency virus type 1 propagation. New Zealand Medical Journal, 111, 222-224.

Tate, W. P., Poole, E. S., Horsfield, J. A., Mannering, S. A., Brown, C. M., Moffat, J. G., Dalphin, M. E., … Major, L. L., & Wilson, D. N. (1995). Translational termination efficiency in both bacteria and mammals is regulated by the base following the stop codon. Biochemistry & Cell Biology, 73, 1095-1103. doi: 10.1139/o95-118

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Journal - Research Other

Chatterjee, A., Stockwell, P. A., Horsfield, J. A., Morison, I. M., & Nakagawa, S. (2014). Base-resolution DNA methylation landscape of zebrafish brain and liver [Data in brief]. Genomics Data, 2, 342-344. doi: 10.1016/j.gdata.2014.10.008

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Journal - Professional & Other Non-Research Articles

Kline, A. D., Krantz, I. D., Bando, M., Shirahige, K., Chea, S., Sakata, T., … Horsfield, J. A., … Haaland, R. E. (2019). Cornelia de Lange syndrome, related disorders, and the Cohesin complex: Abstracts from the 8th Biennial Scientific and Educational Symposium 2018 [Conference report]. American Journal of Medical Genetics Part A. Advance online publication. doi: 10.1002/ajmg.a.61108

Kline, A. D., Calof, A. L., Schaaf, C. A., Krantz, I. D., Jyonouchi, S., Yokomori, K., … Horsfield, J., … Dorsett, D. (2014). Cornelia de Lange syndrome: Further delineation of phenotype, cohesin biology and educational focus, 5th Biennial Scientific and Educational Symposium abstracts [Conference report]. American Journal of Medical Genetics Part A, 164(6), 1384-1393. doi: 10.1002/ajmg.a.36417

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Conference Contribution - Published proceedings: Abstract

Meier, M., Grant, J., Dowdle, A. E., Thomas, A., Gerton, J., Collas, P., … Horsfield, J. A. (2019). A zebrafish model for cohesin function in early embryo development. American Journal of Medical Genetics Part A, Advance online publication. doi: 10.1002/ajmg.a.61108

Leask, M., Merriman, T. R., Dowdle, A., Salvesen, H., Topless, R., Fadason, T., Wei, W., … Horsfield, J. (2018). Non-coding urate-associated variants function in a conserved lincrna regulatory domain that alters MAF transcription. Arthritis & Rheumatology, 70(9, Suppl.), 2238. doi: 10.1002/art.40700

Schuster, K., Leeke, B., Meier, M., Wang, Y., Newman, T., Burgess, S., & Horsfield, J. A. (2017). Neural crest origin for cohesinopathy heart defects. American Journal of Medical Genetics Part A, 173(5), (pp. 1178). doi: 10.1002/ajmg.a.38161

Chin, A., Antony, J., & Horsfield, J. (2017). Identifying synthetic lethal drugs for cohesin mutant AML. Proceedings of the 38th Annual Lorne Genome Conference. Retrieved from http://genome-2017.m.asnevents.com.au/

Dowdle, A., Gerton, J., O'Sullivan, J., & Horsfield, J. (2017). Coming of age: The role of nuclear structure in zygotic genome activation. Proceedings of the Annual Conference of the Genetics Society of Australasia (GSA) with the New Zealand Society for Biochemistry and Molecular Biology (NZSBMB). 540. Retrieved from http://gen2017.w.events4you.currinda.com

Merriman, T. R., Ketharnathan, S., Boocock, J., Phipps-Green, A., Antony, J., Leask, M., … Horsfield, J. (2017). A non-coding genetic variant maximally associated with serum urate levels is functionally linked to HNF4A-dependent PDZK1 expression. Arthritis & Rheumatology, 69(Suppl. 10), 2897. doi: 10.1002/art.40321

Cucco, F., Kline, A. D., Krantz, I. D., Horsfield, J., & Musio, A. (2017). Effects of anti-oxidant treatment on genome stability and in vitro growth of Cornelia de Lange syndrome cell lines. American Journal of Medical Genetics Part A, 173A(5), (pp. 1183-1184). doi: 10.1002/ajmg.a.38161

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