2023
Journal - Research Article
Adhikari, P. B., Zhu, S., Liu, X., Huang, C., Xie, L., Wu, X., … Peters, B., Brownfield, L., … Kasahara, R. D. (2023). Discovery of a cis-regulatory element SaeM involved in dynamic regulation of synergid-specific MYB98. Frontiers in Plant Science, 14, 1177058. doi: 10.3389/fpls.2023.1177058
Harris, C., Hall, M., Arrowfield, R., Herridge, R., Eady, C., Macknight, R., & Brownfield, L. (2023). Assessing inbreeding in perennial ryegrass (Lolium perenne) as a step towards F1 hybrid breeding. Plant Breeding. Advance online publication. doi: 10.1111/pbr.13099
Le Lievre, L., Padinjare Chakkatu, S., Varghese, S., Day, R. C., Pilkington, S. M., & Brownfield, L. (2023). RNA-seq analysis of synchronized developing pollen isolated from a single anther. Frontiers in Plant Science, 14, 1121570. doi: 10.3389/fpls.2023.1121570
Yi, J., Kradolfer, D., Brownfield, L., Ma, Y., Piskorz, E., Köhler, C., & Jiang, H. (2023). Meiocyte size is a determining factor for unreduced gamete formation in Arabidopsis thaliana. New Phytologist, 237, 1179-1187. doi: 10.1111/nph.18473
Conference Contribution - Published proceedings: Abstract
Brownfield, L. (2023). Plant reproduction: The key to crop domestication [Keynote]. Proceedings of the Genetics Otago (GO) Annual Symposium & Workshops. Retrieved from https://blogs.otago.ac.nz/go
2022
Journal - Research Article
Herridge, R., McCourt, T., Jacobs, J. M. E., Mace, P., Brownfield, L., & Macknight, R. (2022). Identification of the genes at S and Z reveals the molecular basis and evolution of grass self-incompatibility. Frontiers in Plant Science, 13, 1011299. doi: 10.3389/fpls.2022.1011299
Journal - Research Other
Brownfield, L. (2022). Pollen helps reveal a role for DC1 domain proteins. Plant & Cell Physiology, 63(12), 1761-1763. doi: 10.1093/pcp/pcac148
Tahir, J., Crowhurst, R., Deroles, S., Hilario, E., Deng, C., Schaffer, R., Le Lievre, L., … Brownfield, L. R., … Pilkington, S. M. (2022). First chromosome-scale assembly and deep floral-bud transcriptome of a male kiwifruit [Data report]. Frontiers in Genetics, 13, 852161. doi: 10.3389/fgene.2022.852161
Conference Contribution - Published proceedings: Abstract
Redmond, E. R., & Brownfield, L. R. (2022). Characterizing a non-heading phenotype found in the ryegrass cultivar 'array'. Proceedings of the Genetics Otago (GO) Annual Symposium. Retrieved from https://blogs.otago.ac.nz/go
Harris, C. R., Eady, C. C., MacKnight, R. C., & Brownfield, L. R. (2022). Assessing the impact of self-fertility on ryegrass. Proceedings of the ComBio Conference. (pp. 47). Retrieved from https://combio.org.au/combio2022/
Conference Contribution - Verbal presentation and other Conference outputs
Brownfield, L. (2022, August). Changing the ins and outs of plant breeding. Verbal presentation at the Applied Genetics/Genomics in Breeding Technologies Satellite Meeting: Queenstown Research Week, Queenstown, New Zealand.
Fakoya, A., Herridge, R., Macknight, R., & Brownfield, L. (2022, August). Optimizing long-read amplicon sequencing to survey intra-cultivar genetic variation in flowering genes in perennial ryegrass. Verbal presentation at the Applied Genetics/Genomics in Breeding Technologies Satellite Meeting: Queenstown Research Week, Queenstown, New Zealand.
2021
Journal - Research Article
Herridge, R., Samarth, Brownfield, L., & Macknight, R. (2021). Identification and characterization of perennial ryegrass (Lolium perenne) vernalization genes. Frontiers in Plant Science, 12, 640324. doi: 10.3389/fpls.2021.640324
Rossig, C., Le Lievre, L., Pilkington, S. M., & Brownfield, L. (2021). A simple and rapid method for imaging male meiotic cells in anthers of model and non-model plant species [Method paper]. Plant Reproduction, 34, 37-46. doi: 10.1007/s00497-021-00404-5
Journal - Research Other
Brownfield, L. (2021). Plant breeding: Revealing the secrets of cytoplasmic male sterility in wheat [Dispatch]. Current Biology, 31(11), R724-R726. doi: 10.1016/j.cub.2021.04.026
Conference Contribution - Published proceedings: Abstract
Herridge, R., Samarth, Fakoya, A., Lee, R., Macknight, R., & Brownfield, L. (2021). Non-heading ryegrass. Proceedings of the 16th Congress of the Federation of Asian & Oceanic Biochemists & Molecular Biologists (FAOBMB). (pp. 249). Retrieved from https://www.faobmb2021.org
Le Lievre, L., Pilkington, S., & Brownfield, L. (2021). Understanding the cause of pollen abortion in female kiwifruit. Proceedings of the 16th Congress of the Federation of Asian & Oceanic Biochemists & Molecular Biologists (FAOBMB). (pp. 247). Retrieved from https://www.faobmb2021.org
Harris, C., Eady, C., Macknight, R., & Brownfield, L. (2021). Assessing the impact of self-fertility in ryegrass. Proceedings of the Genetics Otago (GO) Annual Symposium. Retrieved from https://blogs.otago.ac.nz/go
McCourt, T., Herridge, R., Mace, P., & Brownfield, L. (2021). The role of DUF247 in ryegrass self-incompatibility. Proceedings of the Genetics Otago (GO) Annual Symposium. Retrieved from https://blogs.otago.ac.nz/go
2020
Journal - Research Article
Herridge, R. P., Macknight, R. C., & Brownfield, L. R. (2020). Prospects for F1 hybrid production in ryegrass. New Zealand Journal of Agricultural Research, 63(3), 405-415. doi: 10.1080/00288233.2018.1559867
2019
Chapter in Book - Research
Cridge, A. G., Dearden, P. K., & Brownfield, L. R. (2019). The developmental hourglass in the evolution of embryogenesis. In L. N. de la Rosa & G. Müller (Eds.), Evolutionary developmental biology: A reference guide. Cham, Switzerland: Springer. doi: 10.1007/978-3-319-33038-9
2018
Conference Contribution - Poster Presentation (not in published proceedings)
Herridge, R., Richardson, K., Bryan, G., Brownfield, L. R., & MacKnight, R. C. (2018, August). Variation at S- and Z-genes in ryegrass. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.
Le Lievre, L. G., Pilkington, S. M., & Brownfield, L. R. (2018, August). Understanding pollen abortion in female kiwifruit. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.
Padinjare Chakkatu, S., Day, R., Harrop, T., & Brownfield, L. (2018, August). Low-input transcriptomics of developing pollen isolated from a single anther. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.
Conference Contribution - Verbal presentation and other Conference outputs
Peters, B., Zhorab, S., Casey, J., Twell, D., & Brownfield, L. (2018, August). Male germ-line specification in flowering plants. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.
2017
Chapter in Book - Research
Twell, D., & Brownfield, L. (2017). Analysis of fluorescent reporter activity in the male germline during pollen development by confocal microscopy. In A. Schmidt (Ed.), Plant germline development: Methods and protocols: Methods in molecular biology (Vol. 1669). (pp. 67-75). Springer. doi: 10.1007/978-1-4939-7286-9_6
Peters, B., Aidley, J., Cadzow, M., Twell, D., & Brownfield, L. (2017). Identification of cis-regulatory modules that function in the male germline of flowering plants. In A. Schmidt (Ed.), Plant germline development: Methods and protocols: Methods in molecular biology (Vol. 1669). (pp. 275-293). Springer. doi: 10.1007/978-1-4939-7286-9_22
Journal - Research Article
Cabout, S., Leask, M. P., Varghese, S., Yi, J., Peters, B., Conze, L. L., … Brownfield, L. (2017). The meiotic regulator JASON utilizes alternative translation initiation sites to produce differentially localized forms. Journal of Experimental Botany, 68(15), 4205-4217. doi: 10.1093/jxb/erx222
Peters, B., Casey, J., Aidley, J., Zohrab, S., Borg, M., Twell, D., & Brownfield, L. (2017). A conserved cis-regulatory module determines germline fate through activation of the transcription factor DUO1 promoter. Plant Physiology, 173, 280-293. doi: 10.1104/pp.16.01192
2016
Chapter in Book - Research
Brownfield, L., & Twell, D. (2016). Plant Reproduction. In eLS. Chichester, UK: John Wiley & Sons. doi: 10.1002/9780470015902.a0002046.pub2
Journal - Research Article
Cridge, A. G., Dearden, P. K., & Brownfield, L. R. (2016). Convergent occurrence of the developmental hourglass in plant and animal embryogenesis? Annals of Botany, 117(5), 833-843. doi: 10.1093/aob/mcw024
Conference Contribution - Poster Presentation (not in published proceedings)
Peters, B. A., Casey, J., Zohrab, S., & Brownfield, L. (2016, August-September). Identification of promoter elements that determine male germline fate through activation of DUO1 in eudicots. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.
2015
Journal - Research Article
Brownfield, L., Yi, J., Jiang, H., Minina, E. A., Twell, D., & Köhler, C. (2015). Organelles maintain spindle position in plant meiosis. Nature Communications, 6, 6492. doi: 10.1038/ncomms7492
2014
Conference Contribution - Verbal presentation and other Conference outputs
Brownfield, L., Li, J., Twell, D., & Köhler, C. (2014, July). JASON maintains cytoplasmic organization during male meiosis. Verbal presentation at the 23rd International Congress on Sexual Plant Reproduction (ICSPR), Porto, Portugal.
Brownfield, L. (2014, August). DUO1: A key to unlock male germ-line development in flowering plants. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.
Brownfield, L. (2014, August). Organelles maintain spindle position in plants meiosis. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.
2011
Journal - Research Article
Brownfield, L., & Köhler, C. (2011). Unreduced gamete formation in plants: Mechanisms and prospects. Journal of Experimental Botany, 62(5), 1659-1668. doi: 10.1093/jxb/erq371
Borg, M., Brownfield, L., Khatab, H., Sidorova, A., Lingaya, M., & Twell, D. (2011). The R2R3 MYB transcription factor DUO1 activates a male germline-specific regulon essential for sperm cell differentiation in Arabidopsis. Plant Cell, 23(2), 534-549. doi: 10.1105/tpc.110.081059
2010
Conference Contribution - Poster Presentation (not in published proceedings)
Köhler, C., Erilova, A., Brownfield, L., & Exner, V. (2010, August). Postzygotic reproductive isolation mechanisms acting in the endosperm. Poster session presented at the XXI International Congress on Sexual Plant Reproduction (ICSPR), Bristol, UK.
Borg, M., Brownfield, L., Hafidh, S., Sidorova, A., & Twell, D. (2010, August). Analysis of the DUO1 regulatory network controlling male germline development. Poster session presented at the XXI International Congress on Sexual Plant Reproduction (ICSPR), Bristol, UK.
Kradolfer, D., Brownfield, L., & Köhler, C. (2010, August). Elucidating mechanisms of unreduced male gamete formation. Poster session presented at the XXI International Congress on Sexual Plant Reproduction (ICSPR), Bristol, UK.
Brownfield, L. (2010, August). Mechanisms of unreduced gamete formation during male meiosis. Poster session presented at the XXI International Congress on Sexual Plant Reproduction (ICSPR), Bristol, UK.
Conference Contribution - Verbal presentation and other Conference outputs
Brownfield, L. (2010, February). Exploring unreduced male gamete formation for polyploid production in plant breeding. Verbal presentation at the Green Plant Breeding Technologies International Conference, Vienna, Austria.
Intellectual Property
Koehler, C., Brownfield, L., & Erilova, A. (2010). Polyploid plants. (WO 2010/149322 A1).
2009
Chapter in Book - Research
Brownfield, L., Doblin, M. A., Fincher, G. B., & Bacic, A. (2009). Biochemical and molecular properties of biosynthetic enzymes for (1,3)-ß-glucans in embryophytes, chlorophytes and rhodophytes. In A. Bacic, G. B. Fincher & B. A. Stone (Eds.), Chemistry, biochemistry, and biology of (1→3)-β-glucans and related polysaccharides. (pp. 283-326). Burlington, MA: Academic Press.
Journal - Research Article
Schoft, V. K., Chumak, N., Mosiolek, M., Slusarz, L., Komnenovic, V., Brownfield, L., … Tamaru, H. (2009). Induction of RNA-directed DNA methylation upon decondensation of constitutive heterochromatin. EMBO Reports, 10(9), 1015-1021. doi: 10.1038/embor.2009.152
Erilova, A., Brownfield, L., Exner, V., Rosa, M., Twell, D., Mittelsten Scheid, O., … Köhler, C. (2009). Imprinting of the Polycomb group gene MEDEA serves as a ploidy sensor in Arabidopsis. PLoS Genetics, 5(9), e1000663. doi: 10.1371/journal.pgen.1000663
Brownfield, L., Hafidh, S., Durbarry, A., Khatab, H., Sidorova, A., Doerner, P., & Twell, D. (2009). Arabidopsis DUO POLLEN3 is a key regulator of male germline development and embryogenesis. Plant Cell, 21(7), 1940-1956. doi: 10.1105/tpc.109.066373
Brownfield, L., Hafidh, S., Borg, M., Sidorova, A., Mori, T., & Twell, D. (2009). A plant germline-specific integrator of sperm specification and cell cycle progression. PLoS Genetics, 5(3), e1000430. doi: 10.1371/journal.pgen.1000430
Borg, M., Brownfield, L., & Twell, D. (2009). Male gametophyte development: A molecular perspective. Journal of Experimental Botany, 60(5), 1465-1478. doi: 10.1093/jxb/ern355
Journal - Research Other
Brownfield, L., & Twell, D. (2009). A dynamic DUO of regulatory proteins coordinates gamete specification and germ cell mitosis in the angiosperm male germline [Article addendum]. Plant Signaling & Behavior, 4(12), 1159-1162.
2008
Journal - Research Article
Töller, A., Brownfield, L., Neu, C., Twell, D., & Schulze-Lefert, P. (2008). Dual function of Arabidopsis glucan synthase-like genes GSL8 and GSL10 in male gametophyte development and plant growth. Plant Journal, 54(5), 911-923. doi: 10.1111/j.1365-313X.2008.03462.x
Kim, H. J., Oh, S. A., Brownfield, L., Hong, S. H., Ryu, H., Hwang, I., … Nam, H. G. (2008). Control of plant germline proliferation by SCFFBL17 degradation of cell cycle inhibitors. Nature, 455(7216), 1134-1137. doi: 10.1038/nature07289
Brownfield, L., Wilson, S., Newbigin, E., Bacic, A., & Read, S. (2008). Molecular control of the glucan synthase-like protein NaGSL1 and callose synthesis during growth of Nicotiana alata pollen tubes. Biochemical Journal, 414(1), 43-52. doi: 10.1042/bj20080693
2007
Journal - Research Article
Brownfield, L., Ford, K., Doblin, M. S., Newbigin, E., Read, S., & Bacic, A. (2007). Proteomic and biochemical evidence links the callose synthase in Nicotiana alata pollen tubes to the product of the NaGSL1 gene. Plant Journal, 52(1), 147-156. doi: 10.1111/j.1365-313X.2007.03219.x
Conference Contribution - Verbal presentation and other Conference outputs
Brownfield, L. (2007, June). Regulatory proteins, DUO1 and DUO3, link cell cycle progression and cell specification in male germ line development. Verbal presentation at the 18th International Conference on Arabidopsis Research, Beijing, China.
2006
Journal - Research Article
Farrokhi, N., Burton, R. A., Brownfield, L., Hrmova, M., Wilson, S. M., Bacic, A., & Fincher, G. B. (2006). Plant cell wall biosynthesis: Genetic, biochemical and functional genomics approaches to the identification of key genes. Plant Biotechnology Journal, 4(2), 145-167. doi: 10.1111/j.1467-7652.2005.00169.x