Contact Details
- Phone
- +64 3 479 4929
- debbie.hay@otago.ac.nz
- Position
- Professor
- Department
- Department of Pharmacology and Toxicology
- Qualifications
- BSc(Hons) PhD
Research
After 18 years at the University of Auckland, Debbie moved to the University of Otago in 2020. Research in the Hay laboratory focuses on G protein-coupled receptors (GPCRs). These proteins are the cellular targets of natural hormones and are some of the most important drug targets. Her research aims to contribute to the development of medicines to treat migraine, among other conditions. Debbie is passionate about raising the profile of migraine in New Zealand and improving the lives of patients who have this condition.
Work in the lab spans a range of areas with local and international collaborators in academia and industry, including: pharmacology, medicinal chemistry, peptide chemistry, structural biology, molecular biology, immunohistochemistry and culture of cell lines and primary cells. A particular focus of the lab is class B GPCRs and their interactions with accessory proteins known as “receptor activity-modifying proteins”.
Debbie is a well-cited researcher (Web of Science, Highly Cited), who was elected Fellow of the British Pharmacological Society in 2018 (FBPhS). Other honours include the Gavin and Ann Kellaway Medical Research Fellowship for senior medical researchers (2018), an RSNZ James Cook Research Fellowship (2017), the prestigious 2015 Tregear Award (Australian Peptide Association), the 2013 Novartis Prize (British Pharmacological Society) and the 2013 Custom Science NZSBMB award.
She is an Editorial Board member of the British Journal of Pharmacology since 2015 (Editor Performance Award 2016), and Corresponding Member of the International Union of Basic and Clinical Pharmacology Committee on Receptor Nomenclature and Drug Classification (NC-IUPHAR), chairing its subcommittee on nomenclature of CGRP peptide family receptors. This committee provides influential educational resources for pharmacologists and non-pharmacologists, the pharmaceutical industry and academia.
Debbie is an active mentor of young researchers, is a British Pharmacological Society Women in Pharmacology mentor (2012-present), and has given many invited talks on mentoring, such as 'Mentors: what are they and how do I find one', 'Keep calm and write a paper', 'Keep calm and write a grant application', for early career researchers.
Publications
Tasma, Z., Siow, A., Harris, P. W. R., Brimble, M. A., Hay, D. L., & Walker, C. S. (2025). Development and pharmacological characterization of novel multi- calcitonin gene-related peptide and pituitary adenylate cyclase-activating peptide receptor antagonists. Headache. Advance online publication. doi: 10.1111/head.14916 Journal - Research Article
Garelja, M. L., Alexander, T. I., Walker, C. S., & Hay, D. L. (2024). Extracellular bimolecular fluorescence complementation for investigating membrane protein dimerization: A proof of concept using class B GPCRs. Bioscience Reports. Advance online publication. doi: 10.1042/bsr20240449 Journal - Research Article
Ashina, H., Christensen, R. H., Hay, D. L., Pradhan, A. A., Hoffmann, J., Reglodi, D., … Ashina, M. (2024). Pituitary adenylate cyclase-activating polypeptide signalling as a therapeutic target in migraine. Nature Reviews Neurology. Advance online publication. doi: 10.1038/s41582-024-01011-4 Journal - Research Article
Babych, M., Garelja, M. L., Nguyen, P. T., Hay, D. L., & Bourgault, S. (2024). Converting the amyloidogenic islet amyloid polypeptide into a potent nonaggregating peptide ligand by side chain-to-side chain macrocyclization. Journal of the American Chemical Society. Advance online publication. doi: 10.1021/jacs.4c05297 Journal - Research Article
Waite, J., Rea, B., Kuburas, A., Hay, D., Russo, A., & Sowers, L. (2024). The neuropeptide amylin induces diarrhea in mice and the amylin receptor contributes to CGRP-induced diarrhea. Headache, 64(Suppl. 1), (pp. 14-15). doi: 10.1111/head.14716 Conference Contribution - Published proceedings: Abstract
2025
Journal - Research Article
Tasma, Z., Siow, A., Harris, P. W. R., Brimble, M. A., Hay, D. L., & Walker, C. S. (2025). Development and pharmacological characterization of novel multi- calcitonin gene-related peptide and pituitary adenylate cyclase-activating peptide receptor antagonists. Headache. Advance online publication. doi: 10.1111/head.14916
2024
Journal - Research Article
Garelja, M. L., Alexander, T. I., Walker, C. S., & Hay, D. L. (2024). Extracellular bimolecular fluorescence complementation for investigating membrane protein dimerization: A proof of concept using class B GPCRs. Bioscience Reports. Advance online publication. doi: 10.1042/bsr20240449
Ashina, H., Christensen, R. H., Hay, D. L., Pradhan, A. A., Hoffmann, J., Reglodi, D., … Ashina, M. (2024). Pituitary adenylate cyclase-activating polypeptide signalling as a therapeutic target in migraine. Nature Reviews Neurology. Advance online publication. doi: 10.1038/s41582-024-01011-4
Babych, M., Garelja, M. L., Nguyen, P. T., Hay, D. L., & Bourgault, S. (2024). Converting the amyloidogenic islet amyloid polypeptide into a potent nonaggregating peptide ligand by side chain-to-side chain macrocyclization. Journal of the American Chemical Society. Advance online publication. doi: 10.1021/jacs.4c05297
Tasma, Z., Rees, T. A., Guo, S., Tan, S., O'Carroll, S. J., Faull, R. L. M., … Hay, D. L., & Walker, C. S. (2024). Pharmacology of PACAP and VIP receptors in the spinal cord highlights the importance of the PAC1 receptor. British Journal of Pharmacology. Advance online publication. doi: 10.1111/bph.16376
Rees, T. A., Tasma, Z., Garelja, M. L., O'Carroll, S. J., Walker, C. S., & Hay, D. L. (2024). Calcitonin receptor, calcitonin gene-related peptide and amylin distribution in C1/2 dorsal root ganglia. Journal of Headache & Pain, 25, 36. doi: 10.1186/s10194-024-01744-z
Mazzini, G., Le Foll, C., Boyle, C. N., Garelja, M. L., Zhyvoloup, A., Miller, M. E. T., Hay, D. L., … Lutz, T. A. (2024). The processing intermediate of human amylin, pro-amylin(1–48) has in vivo and in vitro bioactivity. Biophysical Chemistry. Advance online publication. doi: 10.1016/j.bpc.2024.107201
Eggertsen, P. P., Palmfeldt, J., Schytz, H. W., Hay, D., Olsen, R. K. J., & Nielsen, J. F. (2024). Serum calcitonin gene-related peptide in patients with persistent post-concussion symptoms, including headache: A cohort study. Journal of Neurology. Advance online publication. doi: 10.1007/s00415-024-12181-y
Garelja, M. L., Alexander, T. I., Bennie, A., Nimick, M., Petersen, J., Walker, C. S., & Hay, D. L. (2024). Pharmacological characterisation of erenumab, Aimovig, at two calcitonin gene-related peptide responsive receptors. British Journal of Pharmacology, 181, 142-161. doi: 10.1111/bph.16218
Journal - Research Other
Hay, D. L. (2024). A future treatment horizon for migraine? Lancet Neurology, 23, 226-227. doi: 10.1016/s1474-4422(24)00032-2
Ashina, M., Hoffmann, J., Ashina, H., Hay, D. L., Flores-Montanez, Y., Do, T. P., … Dodick, D. W. (2024). Pharmacotherapies for migraine and translating evidence from bench to bedside. Mayo Clinic Proceedings, 99(2), 285-299. doi: 10.1016/j.mayocp.2023.07.003
Conference Contribution - Published proceedings: Abstract
Waite, J., Rea, B., Kuburas, A., Hay, D., Russo, A., & Sowers, L. (2024). The neuropeptide amylin induces diarrhea in mice and the amylin receptor contributes to CGRP-induced diarrhea. Headache, 64(Suppl. 1), (pp. 14-15). doi: 10.1111/head.14716
Hay, D., Rizwan, M., Forrester, L., Rees, T., Rea, B., Sowers, L., … Garelja, M. (2024). Subcutaneous administration of calcitonin-gene related peptide or amylin induces non-evoked pain in female rats. Headache, 64(Suppl. 1), (pp. 13). doi: 10.1111/head.14716
Hay, D., Walker, C., Wang, D., Hewson, L., Rees, T., & Banerjee, P. (2024). Pharmacological characterization of ubrogepant and atogepant antagonism in adipocyte cell models. Headache, 64(Suppl. 1), (pp. 11). doi: 10.1111/head.14716
Hay, D., Nimick, M., Garelja, M., Alexander, T., Walker, C., & Banerjee, P. (2024). Pharmacological characterization of ubrogepant and atogepant in cAMP assays at human, rat and mouse calcitonin family receptors in transfected cells. Headache, 64(Suppl. 1), (pp. 11-12). doi: 10.1111/head.14716
Hay, D., Alexander, T., Nimick, M., Garelja, M., Walker, C., & Banerjee, P. (2024). Antagonism by ubrogepant and atogepant of agonist-mediated human CGRP receptor β-arrestin recruitment and internalization in transfected cells. Headache, 64(Suppl. 1), (pp. 3-4). doi: 10.1111/head.14716
2023
Journal - Research Article
Fernando, S. J. A., Wang, Q., Hay, D. L., Bathgate, R. A. D., Shepherd, P. R., & Lee, K. L. (2023). Evidence that RXFP4 is located in enterochromaffin cells and can regulate production and release of serotonin. Bioscience Reports, 43(4), BSR20221956. doi: 10.1042/BSR20221956
Kotliar, I. B., Lorenzen, E., Schwenk, J. M., Hay, D. L., & Sakmar, T. P. (2023). Elucidating the interactome of G protein-coupled receptors and receptor activity-modifying proteins. Pharmacological Reviews, 75, 1-34. doi: 10.1124/pharmrev.120.000180
Alexander, T. I., Tasma, Z., Siow, A., Rees, T. A., Brimble, M. A., Harris, P. W. R., Hay, D. L., & Walker, C. S. (2023). Novel fluorescently labeled PACAP and VIP highlight differences between peptide internalization and receptor pharmacology. ACS Pharmacology & Translational Science, 6, 52-64. doi: 10.1021/acsptsci.2c00124
Russo, A. F., & Hay, D. L. (2023). CGRP physiology, pharmacology, and therapeutic targets: Migraine and beyond. Physiological Reviews, 103, 1565-1644. doi: 10.1152/physrev.00059.2021
Journal - Research Other
Garelja, M. L., Hay, D., Poyner, D. R., & Walker, C. S. (2023). Calcitonin receptors in GtoPdb v.2023.1 [Citation summary]. IUPHAR/BPS Guide to Pharmacology CITE, (1). doi: 10.2218/gtopdb/F11/2023.1
Rees, T. A., Hay, D. L., & Walker, C. S. (2023). Comment on Yoo et al. Amylin protein expression in the rat brain and neuro-2a cells. Int. J. Mol. Sci. 2022, 23, 4348. International Journal of Molecular Sciences, 24, 1058. doi: 10.3390/ijms24021058
Conference Contribution - Published proceedings: Abstract
Alexander, T., Garelja, M., & Hay, D. (2023). Differential interaction of β-arrestin with the CGRP and AMY1 receptors. Cephalalgia, 43(1 Suppl.), (pp. 180). doi: 10.1177/03331024231189112
Rees, T., Tasma, Z., Garelja, M., Walker, C., & Hay, D. (2023). The trigeminal and dorsal root ganglia exhibit differences in calcitonin receptor, CGRP, and amylin expression. Cephalalgia, 43(1 Suppl.), (pp. 177). doi: 10.1177/03331024231189112
Forrester, L. M., Garelja, M. L., Dalldorf, L. K., Dawson, A. M., Leen, A. J., Rees, T. A., & Hay, D. L. (2023). Potential paracrine interactions between CGRP and the AMY1 receptor in the parabrachial nucleus and amygdala of rodents. Cephalalgia, 43(1 Suppl.), (pp. 40-41). doi: 10.1177/03331024231189112
Garelja, M. L., Rees, T. A., & Hay, D. L. (2023). Investigation of CGRP and AMY1 receptor subunit expression using RNA-fluorescence in situ hybridization in migraine associated tissue. Cephalalgia, 43(1 Suppl.), (pp. 32-33). doi: 10.1177/03331024231189112
Leen, A. J., Garelja, M. L., Forrester, L. M., & Hay, D. L. (2023). Identifying a new migraine-relevant receptor: Potential paracrine interaction between calcitonin gene-related peptide and the amylin 1 receptor in the parabrachial nucleus. In T. Milne, M. Anwar, L. Burga, H. Harcombe, M. Garelja, A. Middleton, D. C. Ribeiro, N. Fleming, K. Ogbuehi & A. Bahn (Eds.), Proceedings of the 265th Otago Medical School Research Society (OMSRS) Meeting: Summer Student Speaker Awards. Dunedin, New Zealand: OMSRS. Retrieved from https://ourarchive.otago.ac.nz/handle/10523/12839
Conference Contribution - Verbal presentation and other Conference outputs
Hay, D. (2023, February). Approaches to understanding the calcitonin/CGRP receptor family: Targets for diabetes, migraine and beyond. Verbal presentation at the Maurice Wilkins Centre (MWC) for Molecular Biodiversity Symposium, Dunedin, New Zealand.
2022
Journal - Research Article
Hendrikse, E. R., Rees, T. A., Tasma, Z., Garelja, M. L., Siow, A., Harris, P. W. R., … Hay, D. L. (2022). Characterization of antibodies against receptor activity-modifying protein 1 (RAMP1): A cautionary tale. International Journal of Molecular Sciences, 23, 16035. doi: 10.3390/ijms232416035
Boer, G. A., Hay, D. L., & Tups, A. (2022). Obesity pharmacotherapy: Incretin action in the central nervous system [Review]. Trends in Pharmacological Sciences, 44(1), 50-63. doi: 10.1016/j.tips.2022.11.001
Tasma, Z., Siow, A., Harris, P. W. R., Brimble, M. A., O'Carroll, S. J., Hay, D. L., & Walker, C. S. (2022). PAC1, VPAC1, and VPAC2 receptor expression in rat and human trigeminal ganglia: Characterization of PACAP-responsive receptor antibodies. International Journal of Molecular Sciences, 23, 13797. doi: 10.3390/ijms232213797
Garelja, M. L., & Hay, D. L. (2022). A narrative review of the calcitonin peptide family and associated receptors as migraine targets: Calcitonin gene-related peptide and beyond. Headache, 62(9), 1093-1104. doi: 10.1111/head.14388
Rees, T. A., Russo, A. F., O'Carroll, S. J., Hay, D. L., & Walker, C. S. (2022). CGRP and the calcitonin receptor are co-expressed in mouse, rat and human trigeminal ganglia neurons. Frontiers in Physiology, 13, 860037. doi: 10.3389/fphys.2022.860037
Hendrikse, E. R., Rees, T. A., Tasma, Z., Le Foll, C., Lutz, T. A., Siow, A., … Hay, D. L. (2022). Calcitonin receptor antibody validation and expression in the rodent brain. Cephalalgia, 42(9), 815-826. doi: 10.1177/03331024221084029
Jamaluddin, A., Chuang, C.-L., Williams, E. T., Siow, A., Yang, S. H., Harris, P. W. R., … Hay, D. L., & Loomes, K. M. (2022). Lipidated calcitonin gene-related peptide (CGRP) peptide antagonists retain CGRP receptor activity and attenuate CGRP action in vivo. Frontiers in Pharmacology, 13, 832589. doi: 10.3389/fphar.2022.832589
Cao, J., Belousoff, M. J., Liang, Y.-L., Johnson, R. M., Josephs, T. M., Fletcher, M. M., … Hay, D. L., … Sexton, P. M. (2022). A structural basis for amylin receptor phenotype. Science, 375, eabm9609. doi: 10.1126/science.abm9609
Kobayashi, A., Hamada, M., Yoshida, M.-a., Kobayashi, Y., Tsutsui, N., Sekiguchi, T., … Hay, D. L., … Sakamoto, H. (2022). Vasopressin-oxytocin-type signaling is ancient and has a conserved water homeostasis role in euryhaline marine planarians. Science Advances, 8, eabk0331. doi: 10.1126/sciadv.abk0331
Tasma, Z., Siow, A., Harris, P. W. R., Brimble, M. A., Hay, D. L., & Walker, C. S. (2022). Characterisation of agonist signalling profiles and agonist-dependent antagonism at PACAP-responsive receptors: Implications for drug discovery. British Journal of Pharmacology, 179, 435-453. doi: 10.1111/bph.15700
Garelja, M. L., Bower, R. L., Brimble, M. A., Chand, S., Harris, P. W. R., Jamaluddin, M. A., … Hay, D. L. (2022). Pharmacological characterisation of mouse calcitonin and calcitonin receptor-like receptors reveals differences compared to human receptors. British Journal of Pharmacology, 179, 416-434. doi: 10.1111/bph.15628
Rees, T. A., Hendrikse, E. R., Hay, D. L., & Walker, C. S. (2022). Beyond CGRP: The calcitonin peptide family as targets for migraine and pain. British Journal of Pharmacology, 179, 381-399. doi: 10.1111/bph.15605
Journal - Research Other
Brain, S. D., Russo, A. F., & Hay, D. L. (2022). Calcitonin gene-related peptide: Novel biology and treatments. Frontiers in Physiology, 13, 964568. doi: 10.3389/fphys.2022.964568
Hay, D. L., Walker, C. S., & Harris, P. W. R. (2022). Atogepant (Qulipta®) for migraine prevention [Drug of the month]. Trends in Pharmacological Sciences, 43(8), 701-702. doi: 10.1016/j.tips.2022.03.013
Conference Contribution - Published proceedings: Abstract
Miller, M. E. T., Akter, R., Zhyvoloup, A., Bower, R. L., Abedini, A., Hay, D. L., & Raleigh, D. P. (2022). From barnyard to bedside: Using interspecies variants to inform the rational design of novel analogs of islet amyloid polypeptide. Biophysical Journal, 121(3, Suppl. 1). doi: 10.1016/j.bpj.2021.11.2478
Conference Contribution - Verbal presentation and other Conference outputs
Alexander, T. I., Garelja, M. L., Gingell, J. J., & Hay, D. L. (2022, September). Differential interaction of β-arrestin for two CGRP-responsive receptors. Verbal presentation at the Postgraduate & Early Career Researchers Committee (PERC) & Division of Health Sciences Early & Mid-Career Researcher (EMCR) Joint Research Symposium, Dunedin, New Zealand.
Hay, D. L. (2022, August). Understanding amylin and its receptors for treating obesity and diatetes. Verbal presentation at the Metabolic & Cardiovascular Disease Satellite Meeting: Queenstown Research Week, Queenstown, New Zealand.
Chang, H. H. G., Leask, M. P., Kallingappa, P. K., Merry, T. L., Hotu, C., de Zoysa, J., … Fronius, M., … Hay, D. L., Merriman, T. R., & Davidson, A. J. (2022, August). The CALCRL receptor: A potential new genetic determinant of diabetic kidney disease in NZ. Verbal presentation at the Metabolic & Cardiovascular Disease Satellite Meeting: Queenstown Research Week, Queenstown, New Zealand.
Rees, T. A., Tasma, Z., Hay, D. L., & Walker, C. S. (2022, August-September). Co-expression of CGRP, amylin and the CTR in the dorsal root ganglia and spinal cord of mice, rats and humans. Verbal presentation at the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists (ASCEPT) NZ Annual Scientific Meeting, Queenstown, New Zealand.
Garelja, M. L., Alexander, T., Walker, C. S., & Hay, D. L. (2022, August-September). Extracellular bimolecular fluorescence complementation assays for investigating receptor dimerization: A proof of concept with receptors for calcitonin gene-related peptide. Verbal presentation at the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists (ASCEPT) NZ Annual Scientific Meeting, Queenstown, New Zealand.
Hay, D. (2022, August-September). A new hope: Calcitonin gene-related peptide blocking drugs in migraine. Invited presentation at the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists (ASCEPT) NZ Annual Scientific Meeting, Queenstown, New Zealand.
2021
Journal - Research Article
Alexander, S. P. H., Christopoulos, A., Davenport, A. P., Kelly, E., Mathie, A. A., Peters, J. A., … Hay, D. L., … Ye, R. D. (2021). The concise guide to Pharmacology 2023/24: G protein-coupled receptors. British Journal of Pharmacology, 180(Suppl. 2), S23-S144. doi: 10.1111/bph.16177
Alexander, S. P. H., Christopoulos, A., Davenport, A. P., Kelly, E., Mathie, A., Peters, J. A., … Hay, D. L., … Ye, R. D. (2021). The concise guide to Pharmacology 2021/22: G protein-coupled receptors. British Journal of Pharmacology, 178(Suppl. 1), S27-S156. doi: 10.1111/bph.15538
Ghanizada, H., Al-Karagholi, M. A.-M., Arngrim, N., Mørch-Rasmussen, M., Walker, C. S., Hay, D. L., & Ashina, M. (2021). Effect of adrenomedullin on migraine-like attacks in patients with migraine: A randomized crossover study. Neurology, 96(20), e2488-e2499. doi: 10.1212/wnl.0000000000011930
Ghanizada, H., Al-Karagholi, M. A.-M., Walker, C. S., Arngrim, N., Rees, T., Petersen, J., … Hay, D. L., & Ashina, M. (2021). Amylin analog pramlintide induces migraine-like attacks in patients. Annals of Neurology, 89(6), 1157-1171. doi: 10.1002/ana.26072
Ashina, M., Terwindt, G. M., Al-Karagholi, M. A.-M., de Boer, I., Lee, M. J., Hay, D. L., … Goadsby, P. J. (2021). Migraine: Disease characterisation, biomarkers, and precision medicine. Lancet, 397, 1496-1504. doi: 10.1016/s0140-6736(20)32162-0
Josephs, T. M., Belousoff, M. J., Liang, Y.-L., Piper, S. J., Cao, J., Garama, D. J., … Hay, D. L., … Sexton, P. M. (2021). Structure and dynamics of the CGRP receptor in apo and peptide-bound forms. Science, 372, eabf7258. doi: 10.1126/science.abf7258
Journal - Research Other
Blumenfeld, A., Durham, P. L., Feoktistov, A., Hay, D. L., Russo, A. F., & Turner, I. (2021). Hypervigilance, allostatic load, and migraine prevention: Antibodies to CGRP or receptor. Neurology & Therapy, 10, 469-497. doi: 10.1007/s40120-021-00250-7
Garelja, M. L., Walker, C. S., & Hay, D. L. (2021). CGRP receptor antagonists for migraine: Are they also AMY1 receptor antagonists? British Journal of Pharmacology, 179, 454-459. doi: 10.1111/bph.15585
Rees, T. A., Hay, D. L., & Walker, C. S. (2021). Amylin antibodies frequently display cross-reactivity with CGRP: Characterization of eight amylin antibodies [Rapid report]. American Journal of Physiology: Regulatory, Integrative & Comparative Physiology, 320, R697-R703. doi: 10.1152/ajpregu.00338.2020
Conference Contribution - Published proceedings: Abstract
Josephs, T., Belousoff, M. J., Liang, Y.-L., Piper, S. J., Cao, J., Garama, D. J., … Hay, D. L., … Sexton, P. M. (2021). G-protein coupled receptor structural dynamics. Proceedings of the 16th Congress of the Federation of Asian & Oceanic Biochemists & Molecular Biologists (FAOBMB). (pp. 46). Retrieved from https://www.faobmb2021.org
Garelja, M., Walker, C., & Hay, D. (2021). Pharmacological characterisation of mouse calcitonin and calcitonin receptor-like receptors reveals differences compared to human receptors. Cephalalgia, 41(1 Suppl.), (pp. 72). doi: 10.1177/03331024211034005
Rees, T., O'Carroll, S., Le Foll, C., Lutz, T., Walker, C., & Hay, D. (2021). CGRP and the AMY1 receptor: Identifying targets for migraine. Cephalalgia, 41(1 Suppl.), (pp. 19-20). doi: 10.1177/03331024211034005
Tasma, Z., Hay, D., & Walker, C. (2021). Profiling PACAP-responsive receptor pharmacology and agonist-dependent antagonism. Cephalalgia, 41(1 Suppl.), (pp. 58). doi: 10.1177/03331024211034005
Conference Contribution - Verbal presentation and other Conference outputs
Hay, D. (2021, December). Neuropeptides and migraines. Verbal presentation at the Pain@Otago Research Symposium, [Online].
2020
Chapter in Book - Research
Chuang, C.-L., & Hay, D. L. (2020). Amylin. In S. Offermanns & W. Rosenthal (Eds.), Encyclopaedia of molecular pharmacology. Cham, Switzerland: Springer. doi: 10.1007/978-3-030-21573-6_10021-1
Pioszak, A. A., & Hay, D. L. (2020). RAMPs as allosteric modulators of the calcitonin and calcitonin-like class B G protein-coupled receptors. In C. J. Langmead (Ed.), From structure to clinical development: Allosteric modulation of G protein-coupled receptors. (pp. 115-141). Cambridge, MA: Academic Press. doi: 10.1016/bs.apha.2020.01.001
Journal - Research Article
Pan, K. S., Siow, A., Hay, D. L., & Walker, C. S. (2020). Antagonism of CGRP signaling by rimegepant at two receptors. Frontiers in Pharmacology, 11, 1240. doi: 10.3389/fphar.2020.01240
Sowers, L. P., Wang, M., Rea, B. J., Taugher, R. J., Kuburas, A., Kim, Y., … Hay, D. L., & Russo, A. F. (2020). Stimulation of posterior thalamic nuclei induces photophobic behavior in mice. Headache, 60, 1961-1981. doi: 10.1111/head.13917
Tasma, Z., Wills, P., Hay, D. L., & Walker, C. S. (2020). Agonist bias and agonist-dependent antagonism at corticotrophin releasing factor receptors. Pharmacology Research & Perspectives, 8(3), e00595. doi: 10.1002/prp2.595
Noh, D., Bower, R. L., Hay, D. L., Zhyvoloup, A., & Raleigh, D. P. (2020). Analysis of amylin consensus sequences suggests that human amylin is not optimized to minimize amyloid formation and provides clues to factors that modulate amyloidogenicity. ACS Chemical Biology, 15(6), 1408-1416. doi: 10.1021/acschembio.9b01050
Liang, Y.-L., Belousoff, M. J., Fletcher, M. M., Zhang, X., Khoshouei, M., Deganutti, G., … Hay, D. L., … Sexton, P. M. (2020). Structure and dynamics of adrenomedullin receptors AM1 and AM2 reveal key mechanisms in the control of receptor phenotype by receptor activity-modifying proteins. ACS Pharmacology & Translational Science, 3(2), 263-284. doi: 10.1021/acsptsci.9b00080
Hendrikse, E. R., Liew, L. P., Bower, R. L., Bonnet, M., Jamaluddin, M. A., Prodan, N., … Hay, D. L. (2020). Identification of small-molecule positive modulators of calcitonin-like receptor-based receptors. ACS Pharmacology & Translational Science, 3(2), 305-320. doi: 10.1021/acsptsci.9b00108
Gingell, J. J., Rees, T. A., Hendrikse, E. R., Siow, A., Rennison, D., Scotter, J., … Hay, D. L. (2020). Distinct patterns of internalization of different calcitonin gene-related peptide receptors. ACS Pharmacology & Translational Science, 3(2), 296-304. doi: 10.1021/acsptsci.9b00089
Garelja, M. L., Au, M., Brimble, M. A., Gingell, J. J., Hendrikse, E. R., Lovell, A., … Hay, D. L. (2020). Molecular mechanisms of class B GPCR activation: Insights from adrenomedullin receptors. ACS Pharmacology & Translational Science, 3(2), 246-262. doi: 10.1021/acsptsci.9b00083
Lu, B. L., Loomes, K. M., Hay, D. L., Harris, P. W. R., & Brimble, M. A. (2020). Synthesis of isotopically labelled αCGRP8-37 and its lipidated analogue. Journal of Labelled Compounds & Radiopharmaceuticals, 63, 325-332. doi: 10.1002/jlcr.3838
Conference Contribution - Published proceedings: Abstract
Hay, D. L. (2020). Discovery and development of novel amylin agonists for obesity and diabetes. Proceedings of the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists (ASCEPT) and the Australasian Pharmaceutical Science Association (APSA) Joint Scientific Meeting: Global Solutions for Global Challenges. Retrieved from https://www.asceptasm.com/2020-annual-scientific-meeting
Rees, T. A., Gingell, J., Siow, A., Harris, P., Brimble, M., Hay, D., & Walker, C. (2020). Differential internalisation of the CGRP and AMY1 receptor. FASEB Journal, 34(Suppl. 1). doi: 10.1096/fasebj.2020.34.s1.02530
Tasma, Z., Hay, D., & Walker, C. (2020). Pharmacological characterisation of PACAP-responsive receptors reveals signalling bias and probe-dependent antagonism. FASEB Journal, 34(Suppl. 1). doi: 10.1096/fasebj.2020.34.s1.02181
2019
Chapter in Book - Research
Hay, D. L. (2019). CGRP receptor biology: Is there more than one receptor? In S. Brain & P. Geppetti (Eds.), Calcitonin gene-related peptide (CGRP) mechanisms: Focus on migraine. (pp. 13-22). Cham, Switzerland: Springer. doi: 10.1007/164_2018_131
Journal - Research Article
Musa, H., Hendrikse, E. R., Brimble, M. A., Garelja, M. L., Watkins, H. A., Harris, P. W. R., & Hay, D. L. (2019). Pharmacological characterization and investigation of N-terminal loop amino acids of adrenomedullin 2 that are important for receptor activation. Biochemistry, 58(32), 3468-3474. doi: 10.1021/acs.biochem.9b00571
Alexander, S. P. H., Christopoulos, A., Davenport, A. P., Kelly, E., Mathie, A., Peters, J. A., … and CGTP Collaborators, including Hay, D. (2019). The concise guide to pharmacology 2019/2020. British Journal of Pharmacology, 176(Suppl. 1), S1-S493. doi: 10.1111/bph.14746
Yule, L. R., Garelja, M. L., Hendrikse, E. R., Gingell, J. J., Poyner, D. R., Harris, P. W. H., Brimble, M. A., & Hay, D. L. (2019). A potent fluorescent calcitonin gene-related peptide analogue enables visualization of receptor internalization. Peptide Science, 111(6), e2416. doi: 10.1002/pep2.24126
Journal - Research Other
Gingell, J. J., Hendrikse, E. R., & Hay, D. L. (2019). New insights into the regulation of CGRP-family receptors. Trends in Pharmacological Sciences, 40(1), 71-83. doi: 10.1016/j.tips.2018.11.005
Hendrikse, E. R., Bower, R. L., Hay, D. L., & Walker, C. S. (2019). Molecular studies of CGRP and the CGRP family of peptides in the central nervous system. Cephalalgia, 39(3), 403-419. doi: 10.1177/0333102418765787
2018
Journal - Research Article
Dal Maso, E., Zhu, Y., Pham, V., Reynolds, C. A., Deganutti, G., Hick, C. A., … Hay, D. L., … Wootten, D. (2018). Extracellular loops 2 and 3 of the calcitonin receptor selectively modify agonist binding and efficacy. Biochemical Pharmacology, 150, 214-244. doi: 10.1016/j.bcp.2018.02.005
Liang, Y.-L., Khoshouei, M., Deganutti, G., Glukhova, A., Koole, C., Peat, T. S., … Hay, D. L., … Sexton, P. M. (2018). Cryo-EM structure of the active, Gs protein complexed, human CGRP receptor. Nature, 561(7724), 492-497. doi: 10.1038/s41586-018-0535-y
Williams, E. T., Harris, P. W. R., Jamaluddin, M. A., Loomes, K. M., Hay, D. L., & Brimble, M. A. (2018). Solid-phase thiol–ene lipidation of peptides for the synthesis of a potent CGRP receptor antagonist. Angewandte Chemie International Edition, 57(36), 11640-11643. doi: 10.1002/anie.201805208
Garelja, M. L., Walker, C. A., Siow, A., Yang, S. H., Harris, P. W. R., Brimble, M. A., … Hay, D. L. (2018). Receptor activity modifying proteins have limited effects on the class B G protein-coupled receptor calcitonin receptor-like receptor stalk. Biochemistry, 57(8), 1410-1422. doi: 10.1021/acs.biochem.7b01180
Booe, J. M., Warner, M. L., Roehrkasse, A. M., Hay, D. L., & Pioszak, A. A. (2018). Probing the mechanism of receptor activity-modifying protein modulation of GPCR ligand selectivity through rational design of potent adrenomedullin and calcitonin gene-related peptide antagonists. Molecular Pharmacology, 93(4), 355-367. doi: 10.1124/mol.117.110916
Simms, J., Uddin, R., Sakmar, T. P., Gingell, J. J., Garelja, M. L., Hay, D. L., … Poyner, D. R. (2018). Photoaffinity cross-linking and unnatural amino acid mutagenesis reveal insights into calcitonin gene-related peptide binding to the CalcitoninRreceptor-Like Receptor/Receptor Activity-Modifying Protein 1 (CLR/RAMP1) complex. Biochemistry, 57(32), 4915-4922. doi: 10.1021/acs.biochem.8b00502
Walker, C. S., Raddant, A. C., Woolley, M. J., Russo, A. F., & Hay, D. L. (2018). CGRP receptor antagonist activity of olcegepant depends on the signalling pathway measured. Cephalalgia, 38(3), 437-451. doi: 10.1177/0333102417691762
Akter, R., Bower, R. L., Abedini, A., Schmidt, A. M., Hay, D. L., & Raleigh, D. P. (2018). Amyloidogenicity, cytotoxicity and receptor activity of bovine amylin: Implications for xenobiotic transplantation and the design of non-toxic amylin variants. ACS Chemical Biology, 13, 2747-2757. doi: 10.1021/acschembio.8b00690
Journal - Research Other
Hay, D. L., Garelja, M. L., Poyner, D. R., & Walker, C. S. (2018). Update on the pharmacology of calcitonin/CGRP family of peptides: IUPHAR Review 25. British Journal of Pharmacology, 175(1), 3-17. doi: 10.1111/bph.14075
Conference Contribution - Published proceedings: Abstract
Walker, C. S., Garelja, M., Rees, T. A., & Hay, D. (2018). Profiling calcitonin gene-related peptide receptor dignalling in transfected cells and trigeminal ganglia neurons. Headache, 58(Suppl. 2), (pp. 69-70). doi: 10.1111/head.13306
2017
Chapter in Book - Research
Gingell, J. J., Walker, C. S., & Hay, D. L. (2017). Class B GPCR: Receptors and RAMPs. In K. Herrick-Davis, G. Milligan & G. Di Giovanni (Eds.), G-protein-coupled receptor dimers. (pp. 289-305). Cham, Switzerland: Humana Press. doi: 10.1007/978-3-319-60174-8_11
Journal - Research Article
Lee, S.-M., Booe, J. M., Gingell, J. J., Sjoelund, V., Hay, D. L., & Pioszak, A. A. (2017). N-glycosylation of asparagine 130 in the extracellular domain of the human calcitonin receptor significantly increases peptide hormone affinity. Biochemistry, 56(26), 3380-3393. doi: 10.1021/acs.biochem.7b00256
Alexander, S. P. H., Kelly, E., Marrion, N. V., Peters, J. A., Faccenda, E., Harding, S. D., … and CGTP Collaborators, including Hay, D. L. (2017). The concise guide to pharmacology 2017/18. British Journal of Pharmacology, 174(Suppl. 1), S1-S446. doi: 10.1111/bph.13987
Woolley, M. J., Reynolds, C. A., Simms, J., Walker, C. S., Mobarec, J. C., Garelja, M. L., … Hay, D. L. (2017). Receptor activity-modifying protein dependent and independent activation mechanisms in the coupling of calcitonin gene-related peptide and adrenomedullin receptors to Gs. Biochemical Pharmacology, 142, 96-110. doi: 10.1016/j.bcp.2017.07.005
Journal - Research Other
Hay, D. L., & Walker, C. S. (2017). CGRP and its receptors. Headache, 57(4), 625-636. doi: 10.1111/head.13064
Hay, D. L. (2017). Amylin. Headache, 57(Suppl. 2), 89-96. doi: 10.1111/head.13077
2016
Journal - Research Article
Watkins, H. A., Chakravarthy, M., Abhayawardana, R. S., Gingell, J. J., Garelja, M. L., Pardamwar, M., … Hay, D. L. (2016). Receptor activity-modifying proteins 2 and 3 generate adrenomedullin receptor subtypes with distinct molecular properties. Journal of Biological Chemistry, 291(22), 11657-11675. doi: 10.1074/jbc.M115.688218
Lee, S.-M., Hay, D. L., & Pioszak, A. A. (2016). Calcitonin and amylin receptor peptide interaction mechanisms: Insights into peptide-binding modes and allosteric modulation of the calcitonin receptor by receptor activity-modifying proteins. Journal of Biological Chemistry, 291(16), 8686-8700. doi: 10.1074/jbc.A115.713628
Gingell, J. J., Simms, J., Barwell, J., Poyner, D. R., Watkins, H. A., Pioszak, A. A., … Hay, D. L. (2016). An allosteric role for receptor activity-modifying proteins in defining GPCR pharmacology. Cell Discovery, 2, 16012. doi: 10.1038/celldisc.2016.12
Bower, R. L., Eftekhari, S., Waldvogel, H. J., Faull, R. L. M., Tajti, J., Edvinsson, L., Hay, D. L., & Walker, C. S. (2016). Mapping the calcitonin receptor in human brain stem. American Journal of Physiology: Regulatory, Integrative & Comparative Physiology, 310(9), R788-R793. doi: 10.1152/ajpregu.00539.2015
Yule, L. R., Bower, R. L., Kaur, H., Kowalczyk, R., Hay, D. L., & Brimble, M. A. (2016). Synthesis and amylin receptor activity of glycomimetics of pramlintide using click chemistry. Organic & Biomolecular Chemistry, 14(23), 5238-5245. doi: 10.1039/c6ob00850j
Bower, R. L., & Hay, D. L. (2016). Amylin structure-function relationships and receptor pharmacology: Implications for amylin mimetic drug development. British Journal of Pharmacology, 173(12), 1883-1898. doi: 10.1111/bph.13496
Journal - Research Other
Walker, C. S., & Hay, D. L. (2016). Reply to comment on: A second trigeminal CGRP receptor: Function and expression of the AMY1 receptor. Annals of Clinical & Translational Neurology, 3(4), 309-310. doi: 10.1002/acn3.294
Hay, D. L., Walker, C. S., Gingell, J. J., Ladds, G., Reynolds, C. A., & Poyner, D. R. (2016). Receptor activity-modifying proteins: Multifunctional G protein-coupled receptor accessory proteins. Biochemical Society Transactions, 44(2), 568-573. doi: 10.1042/BST20150237
Hay, D. L., & Pioszak, A. A. (2016). Receptor activity-modifying proteins (RAMPs): New insights and roles. Annual Review of Pharmacology & Toxicology, 56, 469-487. doi: 10.1146/annurev-pharmtox-010715-103120
2015
Journal - Research Article
Whiting, L., Stewart, K. W., Hay, D. L., Harris, P. W., Choong, Y. S., Phillips, A. R. J., … Cooper, G. J. S. (2015). Glicentin-related pancreatic polypeptide inhibits glucose-stimulated insulin secretion from the isolated pancreas of adult male rats. Physiological Reports, 3(12), e12638. doi: 10.14814/phy2.12638
Alexander, S. P. H., Kelly, E., Marrion, N., Peters, J. A., Benson, H. E., Faccenda, E., … and CGTP Collaborators, including Hay, D. L. (2015). The concise guide to Pharmacology 2015/16: Overview. British Journal of Pharmacology, 172(24), 5729-6202. doi: 10.1111/bph.12931
Booe, J. M., Walker, C. S., Barwell, J., Kuteyi, G., Simms, J., Jamaluddin, M. A., … Hay, D. L. (2015). Structural basis for receptor activity-modifying protein-dependent selective peptide recognition by a G protein-coupled receptor. Molecular Cell, 58(6), 1040-1052. doi: 10.1016/j.molcel.2015.04.018
Walker, C. S., Eftekhari, S., Bower, R. L., Wilderman, A., Insel, P. A., Edvinsson, L., … Hay, D. L. (2015). A second trigeminal CGRP receptor: Function and expression of the AMY1 receptor. Annals of Clinical & Translational Neurology, 2(6), 595-608. doi: 10.1002/acn3.197
Kim, J. S., Brownjohn, P. W., Dyer, B. S., Beltramo, M., Walker, C. S., Hay, D. L., … Tyndall, J. D. A., & Anderson, G. M. (2015). Anxiogenic and stressor effects of the hypothalamic neuropeptide RFRP-3 are overcome by the NPFFR antagonist GJ14. Endocrinology, 156(11), 4152-4162. doi: 10.1210/en.2015-1532
Journal - Research Other
Hay, D. L., Chen, S., Lutz, T. A., Parkes, D. G., & Roth, J. D. (2015). Amylin: Pharmacology, physiology, and clinical potential. Pharmacological Reviews, 67(3), 564-600. doi: 10.1124/pr.115.010629
2014
Journal - Research Article
Walker, C. S., Sundrum, T., & Hay, D. L. (2014). PACAP receptor pharmacology and agonist bias: Analysis in primary neurons and glia from the trigeminal ganglia and transfected cells. British Journal of Pharmacology, 171(6), 1521-1533. doi: 10.1111/bph.12541
Kowalczyk, R., Brimble, M. A., Tomabechi, Y., Fairbanks, A. J., Fletcher, M., & Hay, D. L. (2014). Convergent chemoenzymatic synthesis of a library of glycosylated analogues of pramlintide: Structure-activity relationships for amylin receptor agonism. Organic & Biomolecular Chemistry, 12(41), 8142-8151. doi: 10.1039/c4ob01208a
Walker, C. S., Hay, D. L., Fitzpatrick, S. M., Cooper, G. J. S., & Loomes, K. M. (2014). α-Calcitonin gene related peptide (α-CGRP) mediated lipid mobilization in 3T3-L1 adipocytes. Peptides, 58, 14-19. doi: 10.1016/j.peptides.2014.05.011
Hay, D. L., Harris, P. W. R., Kowalczyk, R., Brimble, M. A., Rathbone, D. L., Barwell, J., … Poyner, D. R. (2014). Structure-activity relationships of the N-terminus of calcitonin gene-related peptide: Key roles of alanine-5 and threonine-6 in receptor activation. British Journal of Pharmacology, 171(2), 415-426. doi: 10.1111/bph.12464
Watkins, H. A., Walker, C. S., Ly, K. N., Bailey, R. J., Barwell, J., Poyner, D. R., & Hay, D. L. (2014). Receptor activity-modifying protein-dependent effects of mutations in the calcitonin receptor-like receptor: Implications for adrenomedullin and calcitonin gene-related peptide pharmacology. British Journal of Pharmacology, 171(3), 772-788. doi: 10.1111/bph.12508
Gingell, J. J., Burns, E. R., & Hay, D. L. (2014). Activity of pramlintide, rat and human amylin but not Aβ1-42 at human amylin receptors. Endocrinology, 155(1), 21-26. doi: 10.1210/en.2013-1658
Conference Contribution - Published proceedings: Abstract
Hay, D. L. (2014). Modulation of class B GPCR pharmacology by RAMPs: Insights from adrenomedullin and amylin receptors. Proceedings of the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists (ASCEPT) and the Molecular Pharmacology of G Protein-Coupled Receptors (MPGPCR) Joint Scientific Meeting. (pp. 40). Retrieved from http://www.asceptasm.com/
Bower, R. L., Kowalczyk, R., Brimble, M. A., & Hay, D. L. (2014). Functional analysis of the N-terminal residues of human amylin. Proceedings of the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists (ASCEPT) and the Molecular Pharmacology of G Protein-Coupled Receptors (MPGPCR) Joint Scientific Meeting. (pp. 87). Retrieved from http://www.asceptasm.com/
Conference Contribution - Poster Presentation (not in published proceedings)
Kutey, G., Barwell, J., Simms, J., Bill, R., Hay, D. L., Gingell, J. J., & Poyner, D. R. (2014, September). The binding of CGRP to the extracellular domain of the CGRP receptor: Mutagenesis and molecular modelling. Poster session presented at the 8th International Symposium on the CGRP Family: CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin, Ascona, Switzerland.
Watkins, H. A., Abhayawardana, R. S., Chakravarthy, M., Barwell, J., Woolley, M. J., Conner, A. C., … Hay, D. L. (2014, September). A complete scan of GPCR extracellular loops reveals RAMP-dependent conformational flexibility. Poster session presented at the 8th International Symposium on the CGRP Family: CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin, Ascona, Switzerland.
Walker, C. S., Hay, D. L., Fitzpatrick, S. M., Cooper, G. J. S., & Loomes, K. M. (2014, September). α-calcitonin gene related peptide (αCGRP) mediated lipid mobilization in 3T3-L1 adipocytes. Poster session presented at the 8th International Symposium on the CGRP Family: CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin, Ascona, Switzerland.
Burns, E. R., Jamaluddin, M. A., & Hay, D. L. (2014, September). Pharmacological characterisation of a stably-transfected AM1 receptor cell line measuring β-arrestin recruitment and cAMP production. Poster session presented at the 8th International Symposium on the CGRP Family: CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin, Ascona, Switzerland.
Bower, R. L., Kowalczyk, R., Brimble, M. A., & Hay, D. L. (2014, September). Functional analysis of the N-terminal residues of human amylin. Poster session presented at the 8th International Symposium on the CGRP Family: CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin, Ascona, Switzerland.
Conference Contribution - Verbal presentation and other Conference outputs
Hay, D. L. (2014, September). Insight into how RAMPs confer adrenomedullin and amylin pharmacology. Verbal presentation at the 8th International Symposium on the CGRP Family: CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin, Ascona, Switzerland.
Walker, C. S., Eftekhari, S., Bower, R. L., Wilderman, A., Insel, P. A., Edvinsson, L., … Hay, D. L. (2014, September). GPCR profiling, pharmacological analysis and histological interrogation reveals two calcitonin gene-related peptide receptors within the trigeminal ganglia. Verbal presentation at the 8th International Symposium on the CGRP Family: CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin, Ascona, Switzerland.
2013
Chapter in Book - Research
Hay, D. L., Conner, A. C., & Poyner, D. R. (2013). Calcitonin gene-related peptide and adrenomedullin receptors. In M. D. Lane & W. J. Lennarz (Eds.), Encyclopedia of biological chemistry. (2nd ed.) (pp. 265-269). London, UK: Academic Press. doi: 10.1016/B978-0-12-378630-2.00363-7
Journal - Research Article
Watkins, H. A., Au, M., Bobby, R., Archbold, J. K., Abdul-Manan, N., Moore, J. M., … Hay, D. L. (2013). Identification of key residues involved in adrenomedullin binding to the AM1 receptor. British Journal of Pharmacology, 169(1), 143-155. doi: 10.1111/bph.12118
Cognard, E., Dargaville, C. G., Hay, D. L., & Shepherd, P. R. (2013). Identification of a pathway by which glucose regulates β-catenin signalling via the cAMP/protein kinase A pathway in β-cell models. Biochemical Journal, 449(3), 803-811. doi: 10.1042/BJ20121454
Kuwasako, K., Hay, D. L., Nagata, S., Murakami, M., Kitamura, K., & Kato, J. (2013). Functions of third extracellular loop and helix 8 of family B GPCRs complexed with RAMPs and characteristics of their receptor trafficking. Current Protein & Peptide Science, 14(5), 416-428. doi: 10.2174/13892037113149990058
Harris, P. W. R., Kowalczyk, R., Hay, D. L., & Brimble, M. A. (2013). A single pseudoproline and microwave solid phase peptide synthesis facilitates an efficient synthesis of human amylin 1-37. International Journal of Peptide Research & Therapeutics, 19(2), 147-155. doi: 10.1007/s10989-012-9325-9
Vohra, S., Taddese, B., Conner, A. C., Poyner, D. R., Hay, D. L., Barwell, J., … Reynolds, C. A. (2013). Similarity between class A and class B G-protein-coupled receptors exemplified through calcitonin gene-related peptide receptor modelling and mutagenesis studies. Journal of the Royal Society Interface, 10(79), 20120846. doi: 10.1098/rsif.2012.0846
Woolley, M. J., Watkins, H. A., Taddese, B., Karakullukcu, Z. G., Barwell, J., Smith, K. J., Hay, D. L., … Conner, A. C. (2013). The role of ECL2 in CGRP receptor activation: A combined modelling and experimental approach. Journal of the Royal Society Interface, 10(88), 20130589. doi: 10.1098/rsif.2013.0589
Qi, T., Dong, M., Wootten, D., Miller, L. J., & Hay, D. L. (2013). Receptor activity-modifying protein-dependent impairment of calcitonin receptor splice variant Δ(1-47)hCT(a) function. British Journal of Pharmacology, 168(3), 644-657. doi: 10.1111/j.1476-5381.2012.02197.x
Journal - Research Other
Watkins, H. A., Rathbone, D. L., Barwell, J., Hay, D. L., & Poyner, D. R. (2013). Structure—activity relationships for α-calcitonin gene-related peptide. British Journal of Pharmacology, 170(7), 1308-1322. doi: 10.1111/bph.12072
Walker, C. S., & Hay, D. L. (2013). CGRP in the trigeminovascular system: A role for CGRP, adrenomedullin and amylin receptors? British Journal of Pharmacology, 170(7), 1293-1307. doi: 10.1111/bph.12129
2012
Chapter in Book - Research
Sexton, P. M., Poyner, D. R., Simms, J., Christopoulos, A., & Hay, D. L. (2012). RAMPs as drug targets. In W. S. Spielman & N. Parameswaran (Eds.), RAMPs. (pp. 61-74). Austin, TX: Landes Bioscience. doi: 10.1007/978-1-4614-2364-5_6
Barwell, J., Wootten, D., Simms, J., Hay, D. L., & Poyner, D. R. (2012). RAMPs and CGRP receptors. In W. S. Spielman & N. Parameswaran (Eds.), RAMPs. (pp. 13-24). Austin, TX: Landes Bioscience. doi: 10.1007/978-1-4614-2364-5_2
Journal - Research Article
Kuwasako, K., Hay, D. L., Nagata, S., Hikosaka, T., Kitamura, K., & Kato, J. (2012). The third extracellular loop of the human calcitonin receptor-like receptor is crucial for the activation of adrenomedullin signalling. British Journal of Pharmacology, 166(1), 137-150. doi: 10.1111/j.1476-5381.2011.01803.x
Halim, A., & Hay, D. L. (2012). The role of glutamic acid 73 in adrenomedullin interactions with rodent AM2 receptors. Peptides, 36(1), 137-141. doi: 10.1016/j.peptides.2012.04.011
Bailey, R. J., Walker, C. S., Ferner, A. H., Loomes, K. M., Prijic, G., Halim, A., … Hay, D. L. (2012). Pharmacological characterization of rat amylin receptors: Implications for the identification of amylin receptor subtypes. British Journal of Pharmacology, 166(1), 151-167. doi: 10.1111/j.1476-5381.2011.01717.x
Journal - Research Other
Bartho, J. D., Ly, K., & Hay, D. L. (2012). Measurement of cAMP in an undergraduate teaching laboratory, using ALPHAscreen technology [Teaching resource]. Science Signaling, 5(211), tr1. doi: 10.1126/scisignal.2002656
Watkins, H. A., Au, M., & Hay, D. L. (2012). The structure of secretin family GPCR peptide ligands: Implications for receptor pharmacology and drug development. Drug Discovery Today, 17(17/18), 1006-1014. doi: 10.1016/j.drudis.2012.05.005
Poyner, D. R., & Hay, D. L. (2012). Secretin family (Class B) G protein-coupled receptors: From molecular to clinical perspectives. British Journal of Pharmacology, 166(1), 1-3. doi: 10.1111/j.1476-5381.2011.01810.x
Watkins, H. A., & Hay, D. L. (2012). Recent progress in unraveling the complexities of receptor biology: Towards new therapeutics [Meeting Report]. Expert Review of Clinical Pharmacology, 5(2), 145-147. doi: 10.1586/ecp.12.11
Hong, Y., Hay, D. L., Quirion, R., & Poyner, D. R. (2012). The pharmacology of adrenomedullin 2/intermedin. British Journal of Pharmacology, 166(1), 110-120. doi: 10.1111/j.1476-5381.2011.01530.x
Barwell, J., Gingell, J. J., Watkins, H. A., Archbold, J. K., Poyner, D. R., & Hay, D. L. (2012). Calcitonin and calcitonin receptor-like receptors: Common themes with family B GPCRs? British Journal of Pharmacology, 166(1), 51-65. doi: 10.1111/j.1476-5381.2011.01525.x
2011
Journal - Research Article
Qi, T., Ly, K., Poyner, D. R., Christopoulos, G., Sexton, P. M., & Hay, D. L. (2011). Structure-function analysis of amino acid 74 of human RAMP1 and RAMP3 and its role in peptide interactions with adrenomedullin and calcitonin gene-related peptide receptors. Peptides, 32(5), 1060-1067. doi: 10.1016/j.peptides.2011.03.004
Journal - Research Other
Archbold, J. K., Flanagan, J. U., Watkins, H. A., Gingell, J. J., & Hay, D. L. (2011). Structural insights into RAMP modification of secretin family G protein-coupled receptors: Implications for drug development. Trends in Pharmacological Sciences, 32(10), 591-600. doi: 10.1016/j.tips.2011.05.007
Hay, D. L., Walker, C. S., & Poyner, D. R. (2011). Adrenomedullin and calcitonin gene-related peptide receptors in endocrine-related cancers: Opportunities and challenges. Endocrine-Related Cancer, 18(1), C1-C14. doi: 10.1677/ERC-10-0244
2010
Edited Book - Research
Hay, D. L., & Dickerson, I. M. (Eds.). (2010). The calcitonin gene-related peptide family: Form, function and future perspectives. Dordrecht, The Netherlands: Springer, 236p. doi: 10.1007/978-90-481-2909-6
Chapter in Book - Research
Barwell, J., Simms, J., Conner, A., Hay, D., Wheatley, M., & Poyner, D. (2010). Ligand binding and activation of the CGRP receptor. In D. L. Hay & I. M. Dickerson (Eds.), The calcitonin gene-related peptide family: Form, function and future perspectives. (pp. 23-40). Dordrecht, The Netherlands: Springer. doi: 10.1007/978-90-481-2909-6_2
Hay, D. L. (2010). Conclusions and future perspectives. In D. L. Hay & I. M. Dickerson (Eds.), The calcitonin gene-related peptide family: Form, function and future perspectives. (pp. 235-236). Dordrecht, The Netherlands: Springer. doi: 10.1007/978-90-481-2909-6_14
Wootten, D. L., Simms, J., Hay, D. L., Christopoulos, A., & Sexton, P. M. (2010). Receptor activity modifying proteins and their potential as drug targets. In C. A. Lunn (Ed.), Membrane proteins as drug targets. (pp. 53-79). London, UK: Academic Press. doi: 10.1016/S1877-1173(10)91003-X
Journal - Research Article
Walker, C. S., Li, X., Whiting, L., Glyn-Jones, S., Zhang, S., Hickey, A. J., … Hay, D. L., … Loomes, K. M. (2010). Mice lacking the neuropeptide α-calcitonin gene-related peptide are protected against diet-induced obesity. Endocrinology, 151(9), 4257-4269. doi: 10.1210/en.2010-0284
Moore, E. L., Gingell, J. J., Kane, S. A., Hay, D. L., & Salvatore, C. A. (2010). Mapping the CGRP receptor ligand binding domain: Tryptophan-84 of RAMP1 is critical for agonist and antagonist binding. Biochemical & Biophysical Research Communications, 394(1), 141-145. doi: 10.1016/j.bbrc.2010.02.131
Gingell, J. J., Qi, T., Bailey, R. J., & Hay, D. L. (2010). A key role for tryptophan 84 in receptor activity-modifying protein 1 in the amylin 1 receptor. Peptides, 31(7), 1400-1404. doi: 10.1016/j.peptides.2010.03.027
Bailey, R. J., Bradley, J. W. I., Poyner, D. R., Rathbone, D. L., & Hay, D. L. (2010). Functional characterization of two human receptor activity-modifying protein 3 variants. Peptides, 31(4), 579-584. doi: 10.1016/j.peptides.2009.12.016
Qi, T., Simms, J., Bailey, R. J., Wheatley, M., Rathbone, D. L., Hay, D. L., & Poyner, D. R. (2010). Structure—function analysis of RAMP1 - RAMP3 chimeras. Biochemistry, 49(3), 522-531. doi: 10.1021/bi9019093
Journal - Research Other
Qi, T., & Hay, D. L. (2010). Structure—function relationships of the N-terminus of receptor activity-modifying proteins. British Journal of Pharmacology, 159(5), 1059-1068. doi: 10.1111/j.1476-5381.2009.00541.x
Walker, C. S., Conner, A. C., Poyner, D. R., & Hay, D. L. (2010). Regulation of signal transduction by calcitonin gene-related peptide receptors. Trends in Pharmacological Sciences, 31(10), 476-483. doi: 10.1016/j.tips.2010.06.006
2009
Journal - Research Article
Robinson, S. D., Aitken, J. F., Bailey, R. J., Poyner, D. R., & Hay, D. L. (2009). Novel peptide antagonists of adrenomedullin and calcitonin gene-related peptide receptors: Identification, pharmacological characterization, and interactions with position 74 in receptor activity-modifying protein 1/3. Journal of Pharmacology & Experimental Therapeutics, 331(2), 513-521. doi: 10.1124/jpet.109.156448
Simms, J., Hay, D. L., Bailey, R. J., Konycheva, G., Bailey, G., Wheatley, M., & Poyner, D. R. (2009). Structure—function analysis of RAMP1 by alanine mutagenesis. Biochemistry, 48(1), 198-205. doi: 10.1021/bi801869n
Journal - Research Other
Sexton, P. M., Poyner, D. R., Simms, J., Christopoulos, A., & Hay, D. L. (2009). Modulating receptor function through RAMPs: Can they represent drug targets in themselves? Drug Discovery Today, 14(7-8), 413-419. doi: 10.1016/j.drudis.2008.12.009
Hay, D. L. (2009). Measurement of phosphorylated extracellular signal - Regulated kinase 1 and 2 in an undergraduate teaching laboratory with alphascreen technology [Teaching resource]. Science Signaling, 2(62), tr1. doi: 10.1126/scisignal.262tr1
Poyner, D. R., Hay, D. L., & Conner, A. C. (2009). CGRP receptor antagonists: Design and screening. Expert Opinion on Drug Discovery, 4(12), 1253-1265. doi: 10.1517/17460440903413496
Hay, D. L., & Poyner, D. R. (2009). Calcitonin gene-related peptide, adrenomedullin and flushing. Maturitas, 64(2), 104-108. doi: 10.1016/j.maturitas.2009.08.011
2008
Chapter in Book - Research
Hay, D. (2008). Calcitonin-type receptor. In S. J. Enna & D. B. Byland (Eds.), xPharm: The comprehensive pharmacology reference. (Online ed.) Elsevier. doi: 10.1016/B978-008055232-3.60175-8
Hay, D. (2008). Calcitonin receptor. In S. J. Enna & D. B. Byland (Eds.), xPharm: The comprehensive pharmacology reference. (Online ed.) Elsevier. doi: 10.1016/B978-008055232-3.60176-X
Hay, D. (2008). Calcitonin gene-related peptide receptor. In S. J. Enna & D. B. Byland (Eds.), xPharm: The comprehensive pharmacology reference. (Online ed.) Elsevier. doi: 10.1016/B978-008055232-3.60177-1
Hay, D. (2008). Amylin receptor. In S. J. Enna & D. B. Byland (Eds.), xPharm: The comprehensive pharmacology reference. (Online ed.) Elsevier. doi: 10.1016/B978-008055232-3.60178-3
Journal - Research Article
Qi, T., Christopoulos, G., Bailey, R. J., Christopoulos, A., Sexton, P. M., & Hay, D. L. (2008). Identification of N-terminal receptor activity-modifying protein residues important for calcitonin gene-related peptide, adrenomedullin, and amylin receptor function. Molecular Pharmacology, 74(4), 1059-1071. doi: 10.1124/mol.108.047142
Danaher, R. N., Loomes, K. M., Leonard, B. L., Whiting, L., Hay, D. L., Xu, L. Y., … Cooper, G. J. S. (2008). Evidence that α-calcitonin gene-related peptide is a neurohormone that controls systemic lipid availability and utilization. Endocrinology, 149(1), 154-160. doi: 10.1210/en.2007-0583
Journal - Professional & Other Non-Research Articles
Hay, D. L., Poyner, D. R., & Quirion, R. (2008). International Union of Pharmacology. LXIX: Status of the calcitonin gene-related peptide subtype 2 receptor [IUPHAR Nomenclature report]. Pharmacological Reviews, 60(2), 143-145. doi: 10.1124/pr.108.00372
2007
Journal - Research Article
Bailey, R. J., & Hay, D. L. (2007). Agonist-dependent consequences of proline to alanine substitution in the transmembrane helices of the calcitonin receptor. British Journal of Pharmacology, 151(5), 678-687. doi: 10.1038/sj.bjp.0707246
Journal - Research Other
Hay, D. L. (2007). What makes a CGRP2 receptor? Clinical & Experimental Pharmacology & Physiology, 34(10), 963-971. doi: 10.1111/j.1440-1681.2007.04703.x
2006
Journal - Research Article
Hay, D. L., Poyner, D. R., & Sexton, P. M. (2006). GPCR modulation by RAMPs. Pharmacology & Therapeutics, 109(1-2), 173-197. doi: 10.1016/j.pharmthera.2005.06.015
Hay, D. L., Christopoulos, G., Christopoulos, A., & Sexton, P. M. (2006). Determinants of 1-piperidinecarboxamide, N-[2-[[5-amino-/-[[4-(4-pyridinyl) -/-piperazinyl]carbonyl]pentyl]amino]-1-[(3,5-dibromo-4-hydroxyphenyl)methyl] -2-oxoethyl]-4-(1,4-dihydro-2-oxo-3(2H)-quinazolinyl) (BIBN4096BS) affinity for calcitonin gene-related peptide and amylin receptors: The role of receptor activity modifying protein 1. Molecular Pharmacology, 70(6), 1984-1991. doi: 10.1124/mol.106.027953
Bailey, R. J., & Hay, D. L. (2006). Pharmacology of the human CGRP1 receptor in Cos 7 cells. Peptides, 27(6), 1367-1375. doi: 10.1016/j.peptides.2005.11.014
Simms, J., Hay, D. L., Wheatley, M., & Poyner, D. R. (2006). Characterization of the structure of RAMP1 by mutagenesis and molecular modeling. Biophysical Journal, 91(2), 662-669. doi: 10.1529/biophysj.106.084582
Saidak, Z., Blake-Palmer, K., Hay, D. L., Northup, J. K., & Glass, M. (2006). Differential activation of G-proteins by μ-opioid receptor agonists. British Journal of Pharmacology, 147(6), 671-680. doi: 10.1038/sj.bjp.0706661
Journal - Research Other
Sexton, P. M., Morfis, M., Tilakaratne, N., Hay, D. L., Udawela, M., Christopoulos, G., & Christopoulos, A. (2006). Complexing receptor pharmacology: Modulation of family B G protein-coupled receptor function by RAMPs. Annals of the New York Academy of Sciences, 1070, 90-104. doi: 10.1196/annals.1317.076
2005
Journal - Research Article
Conner, A. C., Hay, D. L., Simms, J., Howitt, S. G., Schindler, M., Smith, D. M., … Poyner, D. R. (2005). A key role for transmembrane prolines in calcitonin receptor-like receptor agonist binding and signalling: Implications for family B G-protein-coupled receptors. Molecular Pharmacology, 67(1), 20-31. doi: 10.1124/mol.67.1.20
Hay, D. L., & Poyner, D. (2005). The preclinical pharmacology of BIBN4096BS, a CGRP antagonist. Cardiovascular Drug Reviews, 23(1), 31-42. doi: 10.1111/j.1527-3466.2005.tb00155.x
Hay, D. L., Christopoulos, G., Christopoulos, A., Poyner, D. R., & Sexton, P. M. (2005). Pharmacological discrimination of calcitonin receptor: Receptor activity-modifying protein complexes. Molecular Pharmacology, 67(5), 1655-1665. doi: 10.1124/mol.104.008615
2004
Journal - Research Article
Conner, A. C., Simms, J., Hay, D. L., Mahmoud, K., Howitt, S. G., Wheatley, M., & Poyner, D. R. (2004). Heterodimers and family-B GPCRs: RAMPs, CGRP and adrenomedullin. Biochemical Society Transactions, 32(5), 843-846. doi: 10.1042/BST0320843
Hay, D. L., Conner, A. C., Howitt, S. G., Smith, D. M., & Poyner, D. R. (2004). The pharmacology of adrenomedullin receptors and their relationship to CGRP receptors. Journal of Molecular Neuroscience, 22(1-2), 105-113. doi: 10.1385/JMN:22:1-2:105
Journal - Research Other
Udawela, M., Hay, D. L., & Sexton, P. M. (2004). The receptor activity modifying protein family of G protein coupled receptor accessory proteins. Seminars in Cell & Developmental Biology, 15(3), 299-308. doi: 10.1016/j.semcdb.2003.12.019
Hay, D. L., Conner, A. C., Howitt, S. G., Takhshid, M. A., Simms, J., Mahmoud, K., & Poyner, D. R. (2004). The pharmacology of CGRP-responsive receptors in cultured and transfected cells. Peptides, 25(11), 2019-2026. doi: 10.1016/j.peptides.2004.06.007
Conner, A. C., Hay, D. L., & Poyner, D. R. (2004). Characterization of CGRP receptor binding. Current Protocols in Pharmacology, 25(1), 1.30.1-1.30.11. doi: 10.1002/0471141755.ph0130s25
Hay, D. L., Christopoulos, G., Christopoulos, A., & Sexton, P. M. (2004). Amylin receptors: Molecular composition and pharmacology. Biochemical Society Transactions, 32(5), 865-867. doi: 10.1042/BST0320865
2003
Journal - Research Article
Hagner, S., Haberberger, R., Hay, D. L., Facer, P., Reiners, K., Voigt, K., & McGregor, G. P. (2003). Immunohistochemical detection of the calcitonin receptor-like receptor protein in the microvasculature of rat endothelium. European Journal of Pharmacology, 481(2-3), 147-151. doi: 10.1016/j.ejphar.2003.09.030
Hay, D. L., Howitt, S. G., Conner, A. C., Schindler, M., Smith, D. M., & Poyner, D. R. (2003). CL/RAMP2 and CL/RAMP3 produce pharmacologically distinct adrenomedullin receptors: A comparison of effects of adrenomedullin22-52, CGRP8-37 and BIBN4096BS. British Journal of Pharmacology, 140(3), 477-486. doi: 10.1038/sj.bjp.0705472
Journal - Research Other
Hay, D. L., Poyner, D. R., & Smith, D. M. (2003). Desensitisation of adrenomedullin and CGRP receptors. Regulatory Peptides, 112(1-3), 139-145. doi: 10.1016/S0167-0115(03)00032-6
Hay, D. L., Poyner, D. R., & Dickerson, I. (2003). CGRP receptor heterogeneity: A role for receptor component protein? Trends in Endocrinology & Metabolism, 14(1), 3-4. doi: 10.1016/S1043-2760(02)00012-7
2002
Journal - Research Article
Kim, M. S., Small, C. J., Russell, S. H., Morgan, D. G. A., Abbott, C. R., AlAhmed, S. H., Hay, D. L., … Bloom, S. R. (2002). Effects of melanocortin receptor ligands on thyrotropin-releasing hormone release: Evidence for the differential roles of melanocortin 3 and 4 receptors. Journal of Neuroendocrinology, 14(4), 276-282. doi: 10.1046/j.1365-2826.2002.00769.x
Choksi, T., Hay, D. L., Legon, S., Poyner, D. R., Hagner, S., Bloom, S. R., & Smith, D. M. (2002). Comparison of the expression of calcitonin receptor-like receptor (CRLR) and receptor activity modifying proteins (RAMPs) with CGRP and adrenomedullin binding in cell lines. British Journal of Pharmacology, 136(5), 784-792. doi: 10.1038/sj.bjp.0704761
Hay, D. L., Howitt, S. G., Conner, A. C., Doods, H., Schindler, M., & Poyner, D. R. (2002). A comparison of the actions of BIBN4096BS and CGRP8-37 on CGRP and adrenomedullin receptors expressed on SK-N-MC, L6, Col 29 and Rat 2 cells. British Journal of Pharmacology, 137(1), 80-86. doi: 10.1038/sj.bjp.0704844
Journal - Research Other
Conner, A. C., Hay, D. L., Howitt, S. G., Kilk, K., Langel, Ü., Wheatley, M., … Poyner, D. R. (2002). Interaction of calcitonin-gene-related peptide with its receptors. Biochemical Society Transactions, 30(4), 451-455. doi: 10.1042/BST0300451
Smith, D. M., Coppock, H. A., Withers, D. J., Owji, A. A., Hay, D. L., Choksi, T. P., … Poyner, D. R. (2002). Adrenomedullin: Receptor and signal transduction. Biochemical Society Transactions, 30(4), 432-437. doi: 10.1042/BST0300432
2001
Journal - Research Article
Dakin, C. L., Gunn, I., Small, C. J., Edwards, C. M. B., Hay, D. L., Smith, D. M., … Bloom, S. R. (2001). Oxyntomodulin inhibits food intake in the rat. Endocrinology, 142(10), 4244-4250. doi: 10.1210/endo.142.10.8430
Journal - Research Other
Hay, D. L., & Smith, D. M. (2001). Knockouts and transgenics confirm the importance of adrenomedullin in the vasculature [Meeting report]. Trends in Pharmacological Sciences, 22(2), 57-59. doi: 10.1016/S0165-6147(00)01617-5
Hay, D. L., & Smith, D. M. (2001). Adrenomedullin receptors: Molecular identity and function. Peptides, 22(11), 1753-1763. doi: 10.1016/S0196-9781(01)00532-0