The development of zebrafish with specific tissues or organs of interest marked as fluorescent enhances the study of the zebrafish’s embryology, which is already highly visible due to the transparent embryos.
The Otago Zebrafish Facility stocks several lines of fluorescent zebrafish, where different tissues are marked green (GFP) or red (RFP). These can then be studied under the OZF’s Leica M205 FA Fluorescent Stereomicroscope – the highest resolving power and largest zoom range microscope commercially available. The Leica can view fluorescence in three channels – UV, GFP and dsRed.
Through a microscope camera kit, researchers can capture 2- and 3-D imagery for publication purposes and record movie clips.