Associate Professor Craig Marshall

Contact Details

Phone: +64 3 479 7570
Email: craig.marshall@otago.ac.nz

University Links

Position: Associate Professor
Department: Department of Biochemistry
Qualifications: BSc (Hons) PhD (Otago)
Research summary: Biochemistry, physiology and ecology of cold adaptation

Research

Our major goal in this work is to improve our understanding of the structural and functional adaptations of the enzymes that fit them for function in Antarctic fish and how these adaptations compare with those of related fish from more temperate waters. In the long-term we plan to examine a number of proteins so that we can produce a general picture of how low temperature affects protein structure and function. This work will also deepen our understanding of the mechanisms by which ligands interact with enzymes and will improve our knowledge of how proteins fold.

In addition to the general question of how proteins fold to allow activity at low temperatures, we are interested in the specific question of the nature of the structure of antifreeze glycopeptides. We wish to answer this question by growing crystals of these proteins and solving the structure by X-ray crystallography.

Publications

Morris, J., Liddy, M., & Marshall, C. J. (2024). Ice shell purification of ice-active compounds. In R. Drori & C. A. Stevens (Eds.), Ice binding proteins: Methods and protocols: Methods in molecular biology (Vol. 2730). (pp. 25-34). New York, NY: Humana Press. doi: 10.1007/978-1-0716-3503-2_2 Chapter in Book - Research

Morgan-Richards, M., Marshall, C. J., Biggs, P. J., & Trewick, S. A. (2023). Insect freeze-tolerance Downunder: The microbial connection. Insects, 14(1), 89. doi: 10.3390/insects14010089 Journal - Research Article

Wilson, P. W., Marshall, C., Bayer-Giraldi, M., & Mateo, M. A. (2020). Evidence for transition of metastable ice to hexagonal ice in bulk solutions at relatively high temperatures. International Journal of Geosciences, 11(8), 493-500. doi: 10.4236/ijg.2020.118025 Journal - Research Article

Zawierucha, K., Marshall, C. J., Wharton, D., & Janko, K. (2019). A nematode in the mist: Scottnema lindsayae is the only soil metazoan in remote Antarctic deserts, at greater densities with altitude. Polar Research, 38, 1-12. doi: 10.33265/polar.v38.3494 Journal - Research Article

Tate, W. P., & Marshall, C. J. (2018). Post-dormancy transcription and translation in the brine shrimp. In R. A. Browne, P. Sorgeloos & C. N. A. Trotman (Eds.), Artemia biology. (pp. 21-36). Boca Raton, FL: Taylor & Francis. doi: 10.1201/9781351069892 Chapter in Book - Other

2024

Chapter in Book - Research

2023

Journal - Research Article

Morgan-Richards, M., Marshall, C. J., Biggs, P. J., & Trewick, S. A. (2023). Insect freeze-tolerance Downunder: The microbial connection. Insects, 14(1), 89. doi: 10.3390/insects14010089

2020

Journal - Research Article

2019

Journal - Research Article

2018

Chapter in Book - Other

2017

Journal - Research Article

Seybold, A. C., Wharton, D. A., Thorne, M. A. S., & Marshall, C. J. (2017). Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1. Biology Open, 6, 1953-1959. doi: 10.1242/bio.023341

Wharton, D. A., Marshall, C. J., & Egeter, B. (2017). Comparisons between two Antarctic nematodes: Cultured Panagrolaimus sp. DAW1 and field-sourced Panagrolaimus davidi. Nematology, 19(5), 533-542. doi: 10.1163/15685411-00003066

Thorne, M. A. S., Seybold, A., Marshall, C., & Wharton, D. (2017). Molecular snapshot of an intracellular freezing event in an Antarctic nematode. Cryobiology, 75, 117-124. doi: 10.1016/j.cryobiol.2017.01.003

Conference Contribution - Published proceedings: Abstract

Marshall, C., Wharton, D., & Egeter, B. (2017). A new species of Antarctic nematode from Ross Island. Proceedings of the New Zealand Antarctic Science Conference: Challenging science in a challenging environment. (pp. 49). New Zealand Antarctic Research Institute. Retrieved from https://antarctic2017.org

2016

Journal - Research Article

Marshall, C. J., Basu, K., & Davies, P. L. (2016). Ice-shell purification of ice-binding proteins. Cryobiology, 72(3), 258-263. doi: 10.1016/j.cryobiol.2016.03.009

Papetti, C., Windisch, H. S., La Mesa, M., Lucassen, M., Marshall, C., & Lamare, M. D. (2016). Non-Antarctic notothenioids: Past phylogenetic history and contemporary phylogeographic implications in the face of environmental changes. Marine Genomics, 25. doi: 10.1016/j.margen.2015.11.007

Conference Contribution - Published proceedings: Abstract

Papetti, C., Windisch, H., La Mesa, M., Lucassen, M., Marshall, C., & Lamare, M. (2016). Non-Antarctic notothenioids: Past phylogenetic history and contemporary phylogeographic implications in the face of environmental changes. Proceedings of the XXXIV Scientific Committee on Antarctic Research (SCAR) 7th Open Science Conference: Antarctic in the Global Earth System: From the Poles to the Tropics. (pp. 759). [Abstract]

Van de Putte, A. P., Marshall, C., & Janko, K. (2016). The relationship between ecological specialization and response to Pleistocene climate change. Proceedings of the XXXIV Scientific Committee on Antarctic Research (SCAR) 7th Open Science Conference: Antarctic in the Global Earth System: From the Poles to the Tropics. (pp. 481). [Abstract]

2015

Journal - Research Article

Kasparova, E., Van de Putte, A. P., Marshall, C., & Janko, K. (2015). Lifestyle and ice: The relationship between ecological specialization and response to Pleistocene climate change. PLoS ONE, 10(11), e0138766. doi: 10.1371/journal.pone.0138766

2014

Journal - Research Article

MacKenzie, A. L., & Marshall, C. (2014). Proteins in the crystalline styles of the marine mussels Perna Canaliculus Gmelin and Mytilus galloprovincialis Lamarck. Journal of Shellfish Research, 33(3), 673-685. doi: 10.2983/035.033.0302

MacKenzie, A. L., & Marshall, C. (2014). Polysaccharidases in the crystalline styles of selectively bred greenshell mussel (Perna canaliculus Gmelin) families. Journal of Shellfish Research, 33(3), 687-694. doi: 10.2983/035.033.0303

Thorne, M. A. S., Kagoshima, H., Clark, M. S., Marshall, C. J., & Wharton, D. A. (2014). Molecular analysis of the cold tolerant Antarctic nematode, Panagrolaimus davidi. PLoS ONE, 9(8), e104526. doi: 10.1371/journal.pone.0104526

Hawes, T. C., Marshall, C. J., & Wharton, D. A. (2014). A 9kDa antifreeze protein from the Antarctic springtail, Gomphiocephalus hodgsoni. Cryobiology, 69(1), 181-183. doi: 10.1016/j.cryobiol.2014.07.001

Raymond, M. R., Wharton, D. A., & Marshall, C. J. (2014). Nematodes from the Victoria Land coast, Antarctica and comparisons with cultured Panagrolaimus davidi. Antarctic Science, 26(1), 15-22. doi: 10.1017/S0954102013000230

2013

Journal - Research Article

Raymond, M. R., Wharton, D. A., & Marshall, C. J. (2013). Factors determining nematode distributions at Cape Hallett and Gondwana station, Antarctica. Antarctic Science, 25(3), 347-357. doi: 10.1017/S0954102012001162

Conference Contribution - Verbal presentation and other Conference outputs

Wharton, D. A., Raymond, M. R., & Marshall, C. J. (2013, January). Living on the edge: The distribution and phylogenetics of nematodes in Victoria Land, Antarctica. Verbal presentation at the VII Southern Connection Congress: Southern Lands and Southern Oceans: Life on the Edge? Dunedin, New Zealand.

2012

Journal - Research Article

MacKenzie, L. A., Selwood, A. I., & Marshall, C. (2012). Isolation and characterization of an enzyme from the Greenshell™ mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid. Toxicon, 60(3), 406-419. doi: 10.1016/j.toxicon.2012.05.005

Van de Putte, A. P., Janko, K., Kasparova, E., Maes, G. E., Rock, J., Koubbi, P., … Marshall, C. (2012). Comparative phylogeography of three trematomid fishes reveals contrasting genetic structure patterns in benthic and pelagic species. Marine Genomics, 8, 23-34. doi: 10.1016/j.margen.2012.05.002

Hawes, T. C., Marshall, C. J., & Wharton, D. A. (2012). Ultraviolet radiation tolerance of the Antarctic springtail, Gomphiocephalus hodgsoni. Antarctic Science, 24(2), 147-153. doi: 10.1017/S0954102011000812

Conference Contribution - Published proceedings: Abstract

Raymond, M., Marshall, C., & Wharton, D. (2012). Living on the edge: Nematodes in Victoria Land, Antarctica. Proceedings of the Polar Environment Research Theme (PERT) Workshop. Retrieved from http://polarresearch.otago.ac.nz/pert-workshop-2012/

Kumar, A., Knapp, K., Krause, K., & Marshall, C. (2012). Revisiting the structure of lactate dehydrogenase from Spiny Dogfish. Protein Science, 21(S1), (pp. 95). doi: 10.1002/pro.2113

2011

Journal - Research Article

Janko, K., Marshall, C., Musilová, Z., Van Houdt, J., Couloux, A., Cruaud, C., & Lecointre, G. (2011). Multilocus analyses of an Antarctic fish species flock (Teleostei, Notothenioidei, Trematominae): Phylogenetic approach and test of the early-radiation event. Molecular Phylogenetics & Evolution, 60(3), 305-316. doi: 10.1016/j.ympev.2011.03.008

Hawes, T. C., Marshall, C. J., & Wharton, D. A. (2011). Antifreeze proteins in the Antarctic springtail, Gressittacantha terranova. Journal of Comparative Physiology B, 181(6), 713-719. doi: 10.1007/s00360-011-0564-4

Conference Contribution - Poster Presentation (not in published proceedings)

McKellar, J., & Marshall, C. (2011, September). Potent antifreeze proteins from the larvae of the yellow mealworm beetle, Tenebrio molitor. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2010

Journal - Research Article

Wharton, D. A., Selvanesan, L., & Marshall, C. J. (2010). Ice-active proteins from New Zealand snow tussocks, Chionochloa macra and c. Rigida. CryoLetters, 31(3), 239-248.

Conference Contribution - Poster Presentation (not in published proceedings)

Lamare, M., Isely, N., Liddy, M., Marshall, C., & Barker, M. (2010, July-August). Expression of the DNA repair enzyme, photolyase, in the Antarctic sea urchin. Poster session presented at the Scientific Committee on Antarctic Research (SCAR) & Open Science Conference, Buenos Aires, Argentina.

2009

Journal - Research Article

Isely, N., Lamare, M., Marshall, C., & Barker, M. (2009). Expression of the DNA repair enzyme, photolyase, in developmental tissues and larvae, and in response to ambient UV-R in the Antarctic sea urchin Sterechinus neumayeri. Photochemistry & Photobiology, 85(5), 1168-1176. doi: 10.1111/j.1751-1097.2009.00566.x

Wharton, D. A., & Marshall, C. J. (2009). How do terrestrial Antarctic organisms survive in their harsh environment? Journal of Biology, 8, 39. doi: 10.1186/jbiol142

Wharton, D. A., Pow, B., Kristensen, M., Ramløv, H., & Marshall, C. J. (2009). Ice-active proteins and cryoprotectants from the New Zealand alpine cockroach, Celatoblatta quinquemaculata. Journal of Insect Physiology, 55(1), 27-31. doi: 10.1016/j.jinsphys.2008.09.007

Conference Contribution - Published proceedings: Abstract

Liddy, M., Lamare, M., Marshall, C., & Isely, N. (2009). Expression of the DNA repair enzyme, photolyase, in embryos of the Antarctic sea urchin, Sterechinus Neumayeri, in response to increases in UV-R during ozone depletion. Proceedings of the Annual Antarctic Conference. (pp. 100). Christchurch, New Zealand: Antarctica New Zealand. [Abstract]

Sharma, M., Vulin, C., Clarke, S., & Marshall, C. J. (2009). Characterisation of an antifreeze protein from the meal worm, Tenebrio molitor. Proceedings of the ComBio2009 Conference. [CD-ROM] [Abstract]

Clarke, S. R., Marshall, C. J., & Wharton, D. A. (2009). Evidence for a new mechanism for ice active proteins. Proceedings of the ComBio2009 Conference. [CD-ROM] [Abstract]

2008

Journal - Research Article

Kumble, K. D., Demmer, J., Fish, S., Hall, C., Corrales, S., DeAth, A., … Luxmanan, S., Marshall, C. J., & Wharton, D. A. (2008). Characterization of a family of ice-active proteins from the Ryegrass, Lolium perenne. Cryobiology, 57(3), 263-268. doi: 10.1016/j.cryobiol.2008.09.005

Smith, T., Wharton, D. A., & Marshall, C. J. (2008). Cold tolerance of an Antarctic nematode that survives intracellular freezing: Comparisons with other nematode species. Journal of Comparative Physiology B, 178(1), 93-100. doi: 10.1007/s00360-007-0202-3

Conference Contribution - Published proceedings: Abstract

Isely, N., Marshall, C., & Lamare, M. (2008). The enzyme photolyase is induced in echinoderms by exposure to UV light. Proceedings of the Annual Antarctic Conference. (pp. 55). Christchurch, New Zealand: Antarctica New Zealand. [Abstract]

Raymond, M. R., Marshall, C. J., & Wharton, D. A. (2008). Defining habitats for Antarctic nematode species from the Ross Sea region. Proceedings of the Annual Antarctic Conference. (pp. 53). Christchurch, New Zealand: Antarctica New Zealand. [Abstract]

Wharton, D. A., Raymond, M. R., Clarke, S. R., & Marshall, C. J. (2008). An Antarctic nematode that survives intracellular freezing. Proceedings of the Annual Antarctic Conference. (pp. 17). Christchurch, New Zealand: Antarctica New Zealand. [Abstract]

2007

Journal - Research Article

Janko, K., Lecointre, G., DeVries, A., Couloux, A., Cruaud, C., & Marshall, C. (2007). Did glacial advances during the Pleistocene influence differently the demographic histories of benthic and pelagic Antarctic shelf fishes? Inferences from intraspecific mitochondrial and nuclear DNA sequence diversity. BMC Evolutionary Biology, 7, 220. doi: 10.1186/1471-2148-7-220

Wharton, D. A., Wilson, P. W., Mutch, J. S., Marshall, C. J., & Lim, M. (2007). Recrystallization inhibition assessed by splat cooling and optical recrystallometry. CryoLetters, 28(1), 61-68.

2006

Journal - Research Article

Lamare, M. D., Barker, M. F., Lesser, M. P., & Marshall, C. (2006). DNA photorepair in echinoid embryos: Effects of temperature on repair rate in Antarctic and non-Antarctic species. Journal of Experimental Biology, 209, 5017-5028. doi: 10.1242/jeb.02598

2005

Journal - Research Article

Wharton, D. A., Barrett, J., Goodall, G., Marshall, C. J., & Ramløv, H. (2005). Ice-active proteins from the Antarctic nematode Panagrolaimus davidi. Cryobiology, 51, 198-207.

Wharton, D. A., Downes, M. F., Goodall, G., & Marshall, C. J. (2005). Freezing and cryoprotective dehydration in an Antarctic nematode (Panagrolaimus davidi) visualised using a freeze substitution technique. Cryobiology, 50, 21-28.

2004

Journal - Research Article

Wharton, D. A., Mutch, J. S., Wilson, P. W., Marshall, C. J., & Lim, M. (2004). A simple ice nucleation spectrometer. CryoLetters, 25, 335-340.

2003

Journal - Research Article

Wharton, D. A., Goodall, G., & Marshall, C. J. (2003). Freezing survival and cryoprotective dehydration as cold tolerance mechanisms in the Antarctic nematode Panagrolaimus davidi. Journal of Experimental Biology, 206, 215-221.

Conference Contribution - Published proceedings: Abstract

Wharton, D. A., Mutch, J. S., Wilson, P. W., Marshall, C. J., & Lim, M. (2003). A simple ice nucleation spectrometer. Proceedings of the International Symposium on Animal and Plant Cold Hardiness. Czech Republic: TEMP. [Abstract]

2002

Journal - Research Article

Denton, M. J., Marshall, C. J., & Legge, M. (2002). The protein folds as platonic forms: New support for the pre-darwinian conception of evolution by natural law. Journal of Theoretical Biology, 219(3), 325-342.

Wharton, D. A., Goodall, G., & Marshall, C. J. (2002). Freezing rate affects the survival of a short-term freezing stress in Panagrolaimus davidi, an Antarctic nematode that survives intracellular freezing. CryoLetters, 23, 5-10.

2001

Journal - Research Article

Sharpe, M., Love, C., & Marshall, C. (2001). Lactate dehydrogenase from the Antarctic eelpout, Lycodichthys dearborni. Polar Biology, 24(4), 258-269. doi: 10.1007/s003000000206

Denton, M. J., & Marshall, C. J. (2001). Laws of form revisited. Nature, 410, 417.

Denton, M. J., & Marshall, C. J. (2001). Laws of form revisted. Concepts, 410, 417.

Conference Contribution - Published proceedings: Abstract

Goodall, G., Wharton, D. A., Barrett, J., Ramlov, H., & Marshall, C. J. (2001). Ice active compounds and intracellular freezing in an Antarctic nematode (Panagrolaimus davidi). VIII International Biology Symposium, Amsterdam. Scientific Committee for Antarctic Research. [Abstract]

2000

Conference Contribution - Published proceedings: Abstract

Marshall, C. J., Crossman, D., Love, D. R., McInnes, S. J., & Fleming, R. I. (2000). Structural studies of cold-adaptation: Lactate dehydrogenases from notothenioid fish. Antarctic Ecosystems: Models for Wider Ecological Understanding. (pp. 123-133). Christchurch: NZ Sciences. [Abstract]

1998

Journal - Research Article

Piotte, C. P., Hunter, A. K., Marshall, C. J., & Grigor, M. R. (1998). Phylogenetic analysis of three lipocalin-like proteins present in the milk of Trichosurus vulpecula (Phalangeridae, Marsupialia). Journal of Molecular Evolution, 46, 361-369.

1997

Journal - Research Article

Marshall, C. J. (1997). Cold-adapted enzymes. Trends in Biotechnology, 15(9), 359-364.

Piotte, C. P., Marshall, C. J., Hubbard, M. J., Collet, C., & Grigor, M. R. (1997). Lysozyme and α-lactalbumin from the milk of a marsupial, the common brush-tailed possum (Trichosurus vulpecula). Biochimica et Biophysica Acta: General Subjects, 1336(2), 235-242. doi: 10.1016/S0304-4165(97)00033-0

1995

Journal - Research Article

Cutfield, S. M., Dodson, E. J., Anderson, B. F., Marshall, C. J., Moody, P. C., Sullivan, P. A., & Cutfield, J. F. (1995). The crystal structure of a major secreted aspartic proteinase from Candida albicans in complexes with two inhibitors. Structure, 3, 1261-1271.