Dr Nick Magon carried out his PhD at the Centre for Free Radical Research under the supervision of Professors' Mark Hampton, Tony Kettle and Richard Gearry. He investigated inflammatory biomarkers produced as a result of neutrophil activation, primarily using protein purification and mass spectrometry.
After his PhD, he worked as a Postdoctoral Fellow in the Department of Pathology and Immunology at the University of Geneva. There he investigated the molecular mechanisms of red blood cell ageing during cold storage using a range of techniques, including flow cytometry.
His current projects involve using mass spectrometry to characterise oxidative modifications to the neutrophil protein calprotectin that may be useful as biomarkers of inflammation, and to identify proteins that are being oxidatively-modified during the early stages of necroptosis. He is also responsible for maintaining and further developing our commercial oxidative biomarker ELISA kit.
Publications
Paumann-Page, M., Ashby, L. V., Khalilova, I., Magon, N. J., Hofbauer, S., Paton, L. N., … Kettle, A. J. (2023). Hypochlorous acid inactivates myeloperoxidase inside phagocytosing neutrophils. Redox Biochemistry & Chemistry, 5-6, 100008. doi: 10.1016/j.rbc.2023.100008
Journal - Research Article
Major, G., Longoni, A., Simcock, J., Magon, N. J., Harte, J., Bathish, B., Kemp, R., Woodfield, T., & Lim, K. S. (2023). Clinical applicability of visible light-mediated cross-linking for structural soft tissue reconstruction. Advanced Science, 10, 2300538. doi: 10.1002/advs.202300538
Journal - Research Article
Bozonet, S. M., Magon, N. J., Schwartfeger, A. J., Konigstorfer, A., Heath, S. G., Vissers, M. C. M., … Göbl, C., … Hampton, M. B. (2023). Oxidation of caspase-8 by hypothiocyanous acid enables TNF-mediated necroptosis. Journal of Biological Chemistry, 104792. Advance online publication. doi: 10.1016/j.jbc.2023.104792
Journal - Research Article
Magon, N. J., Turner, R., Kettle, A. J., & Winterbourn, C. C. (2023). Cross-linking between cysteine and lysine, tryptophan or tyrosine in peptides and proteins treated with hypochlorous acid and other reactive halogens. Redox Biochemistry & Chemistry. Advance online publication. doi: 10.1016/j.rbc.2023.100002
Journal - Research Article
Morris, V. K., Heath, S. G., O'Connor, K. M., Bozonnet, S., Gray, S. G., Naughton, J. D., Magon, N. J., Smith, B. R., Botha, A. D., … Göbl, C. (2022, August). The tumour suppressor protein p16 forms amyloid structures. Verbal presentation at the Cancer Satellite Meeting: Queenstown Research Week, Queenstown, New Zealand.
Conference Contribution - Verbal presentation and other Conference outputs
Crake, R. L. I., Burgess, E. R., Wiggins, G. A. R., Magon, N. J., Das, A. B., Vissers, M. C. M., Morrin, H. R., Royds, J. A., Slatter, T. L., Robinson, B. A., Phillips, E., & Dachs, G. U. (2022). Ascorbate content of clinical glioma tissues is related to tumour grade and to global levels of 5-hydroxymethyl cytosine. Scientific Reports, 12, 14845. doi: 10.1038/s41598-022-19032-8
Journal - Research Article
Edwards, T. S., Dickerhof, N., Magon, N. J., Paton, L. N., Sly, P. D., & Kettle, A. J. (2022). Formation of calprotectin-derived peptides in the airways of children with cystic fibrosis. Journal of Immunology, 208, 979-990. doi: 10.4049/jimmunol.2001017
Journal - Research Article
Smyth, L. C. D., Murray, H. C., Hill, M., van Leeuwen, E., Highet, B., Magon, N. J., … Mathiesen, S. N., Mockett, B., … Clarkson, A. N., … Abraham, W. C., Hughes, S. M., … Kettle, A. J., … Hampton, M. B. (2022). Neutrophil-vascular interactions drive myeloperoxidase accumulation in the brain in Alzheimer's disease. Acta Neuropathologicca Communications, 10, 38. doi: 10.1186/s40478-022-01347-2
Journal - Research Article
Paumann-Page, M., Kienzl, N. F., Motwani, J., Bathish, B., Paton, L. N., Magon, N. J., … Eccles, M. R., & Winterbourn, C. C. (2021). Peroxidasin protein expression and enzymatic activity in metastatic melanoma cell lines are associated with invasive potential. Redox Biology, 46, 102090. doi: 10.1016/j.redox.2021.102090
Journal - Research Article
Smith-Díaz, C. C., Magon, N. J., McKenzie, J. L., Hampton, M. B., Vissers, M. C. M., & Das, A. B. (2021). Ascorbate inhibits proliferation and promotes myeloid differentiation in TP53-mutant leukemia. Frontiers in Oncology, 11, 709543. doi: 10.3389/fonc.2021.709543
Journal - Research Article
Heath, S. G., Magon, N. J., Winterbourn, C. C., Hampton, M., Morris, V. K., & Göbl, C. (2021). Oxidation induced amyloid formation of the tumour suppressor protein p16. Proceedings of the 16th Congress of the Federation of Asian & Oceanic Biochemists & Molecular Biologists (FAOBMB). (pp. 33). Retrieved from https://www.faobmb2021.org
Conference Contribution - Published proceedings: Abstract
Smith-Díaz, C. C., Magon, N. J., McKenzie, J. L., Hampton, M. B., Vissers, M. C. M., & Das, A. B. (2021). Ascorbate inhibits proliferation and promotes myeloid differentiation in TP53-mutant leukaemia. Proceedings of the 16th Congress of the Federation of Asian & Oceanic Biochemists & Molecular Biologists (FAOBMB). (pp. 183). Retrieved from https://www.faobmb2021.org
Conference Contribution - Published proceedings: Abstract
Magon, N. J., Parker, H. A., Ashby, L. V., Springer, R. J., & Hampton, M. B. (2020). Analysis of neutrophil bactericidal activity. In M. T. Quinn & F. R. DeLeo (Eds.), Neutrophil: Methods and protocols: Methods in molecular biology (Vol. 2087). (3rd ed.) (pp. 149-164). New York, NY: Humana Press. doi: 10.1007/978-1-0716-0154-9_12
Chapter in Book - Research
Meng, Y., Sheen, C. R., Magon, N. J., Hampton, M. B., & Dobson, R. C. J. (2020). Structure-function analyses of alkylhydroperoxidase D from Streptococcus pneumoniae reveal an unusual three-cysteine active site architecture. Journal of Biological Chemistry, 295(10), 2984-2999. doi: 10.1074/jbc.RA119.012226
Journal - Research Article
Magon, N. J., Turner, R., Gearry, R. B., Hampton, M. B., Sly, P. D., & Kettle, A. J. (2015). Oxidation of calprotectin by hypochlorous acid prevents chelation of essential metal ions and allows bacterial growth: Relevance to infections in cystic fibrosis. Free Radical Biology & Medicine, 86, 133-144. doi: 10.1016/j.freeradbiomed.2015.05.022
Journal - Research Article
Wilkie-Grantham, R. P., Magon, N. J., Harwood, D. T., Kettle, A. J., Vissers, M. C., Winterbourn, C. C., & Hampton, M. B. (2015). Myeloperoxidase-dependent lipid peroxidation promotes the oxidative modification of cytosolic proteins in phagocytic neutrophils. Journal of Biological Chemistry, 290(15), 9896-9905. doi: 10.1074/jbc.M114.613422
Journal - Research Article
Parker, H. A., Magon, N. J., Green, J. N., Hampton, M. B., & Winterbourn, C. C. (2014). Analysis of neutrophil bactericidal activity. In M. T. Quinn & F. R. DeLeo (Eds.), Neutrophil methods and protocols: Methods in Molecular Biology (Vol. 1124). (2nd ed.) (pp. 291-306). New York, NY: Springer. doi: 10.1007/978-1-62703-845-4_19
Chapter in Book - Research
Dickerhof, N., Magon, N. J., Tyndall, J. D. A., Kettle, A. J., & Hampton, M. B. (2014). Potent inhibition of macrophage migration inhibitory factor (MIF) by myeloperoxidase-dependant oxidation of epicatechins. Biochemical Journal, 462(2), 303-314. doi: 10.1042/BJ20140612
Journal - Research Article
Magon, N. J., Turner, R., Dickerhof, N., Gearry, R., Hampton, M., Sly, P., & Kettle, A. (2013). Oxidation of methionine in calprotectin by neutrophils in the airways of children with cystic fibrosis. Free Radical Biology & Medicine, 65(Suppl. 2), (pp. S62-S63). doi: 10.1016/j.freeradbiomed.2013.10.535
Conference Contribution - Published proceedings: Abstract
Magon, N. J., Gearry, R. B., Hampton, M. B., & Kettle, A. J. (2012). Oxidised calprotectin as a biomarker for inflammatory disease. Proceedings of the Oxygen Theme Meeting. (pp. 8). Dunedin, New Zealand: University of Otago. Retrieved from https://www.otago.ac.nz/research/otago051864.pdf
Conference Contribution - Published proceedings: Abstract
Tidén, A.-K., Sjögren, T., Svensson, M., Bernlind, A., Senthilmohan, R., Auchère, F., … Forbes, L. V., Magon, N. J., Paton, L. N., Jameson, G. N. L., … Kettle, A. J. (2011). 2-Thioxanthines are mechanism-based inactivators of myeloperoxidase that block oxidative stress during inflammation. Journal of Biological Chemistry, 286(43), 37578-37589. doi: 10.1074/jbc.M111.266981
Journal - Research Article
Magon, N. J., Gearry, R. B., Hampton, M. B., & Kettle, A. J. (2010). Oxidised calprotectin as a biomarker for inflammatory disease. Free Radical Biology and Medicine.49(Suppl. 1), (pp. S169). doi: 10.1016/j.freeradbiomed.2010.10.479
Conference Contribution - Published proceedings: Abstract
Research Fellow