Dr Tanya Cully
Calcium and ROS interactions in healthy and diseased skeletal muscle
My research focus is to quantify the levels and movements of calcium and reactive oxygen species (ROS) within the triadic microdomain and bridge the knowledge gap in calcium and ROS crosstalk.
At rest, these two processes must interact to maintain muscle at the ready for peak performance. However, we currently do not understand how ROS and calcium interact to maintain the system for optimal muscle contraction.
My research aims to determine how calcium, ROS, and their interaction can alter muscle function in health and disease, via regulation of triadic microdomain homeostasis. To achieve this, my lab uses several techniques, including live-cell confocal microscopy of mechanically skinned and intact muscle fibres, western blotting, viral transductions and electroporation.
Gonano, L. A., Aitken-Buck, H. M., Chakraborty, A. D., Worthington, L. P. I., Cully, T. R., Lamberts, R. R., … Jones, P. P. (2022). Regulation of cardiac ryanodine receptor function by the cyclic-GMP dependent protein kinase G. Current Research in Physiology, 5, 171-178. doi: 10.1016/j.crphys.2022.03.004
Cully, T. R., Choi, R. H., Bjorksten, A. R., Stephenson, D. G., Murphy, R. M., & Launikonis, B. S. (2018). Junctional membrane Ca2+ dynamics in human muscle fibers are altered by malignant hyperthermia causative RyR mutation. PNAS, 115(32), 8215-8220. doi: 10.1073/pnas.1800490115
Launikonis, B. S., Cully, T. R., Csernoch, L., & Stephenson, D. G. (2018). NHE- and diffusion-dependent proton fluxes across the tubular system membranes of fast-twitch muscle fibers of the rat. Journal of General Physiology, 150(1), 95-110. doi: 10.1085/jgp.201711891
Loehr, J. A., Wang, S., Cully, T. R., Pal, R., Larina, I. V., Larina, K. V., & Rodney, G. G. (2018). NADPH oxidase mediates microtubule alterations and diaphragm dysfunction in dystrophic mice. eLIFE, 7, e31732.. doi: 10.7554/eLife.31732
Cully, T. R. (2018). Milestones and frontiers in muscular physiology: Ca2+ handling in T-tubules. Iatreia, 31(1, Suppl.), (pp. S-20). [Abstract]