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Client-prepared libraries

The Otago Genomics & Bioinformatics Facility (OGBF) are now delivering services previously facilitated under the NZ Genomics collaboration directly to customers. Sequencing and bioinformatics enquiries can be directed to

Library requirements

We are happy to sequence client prepared Illumina-compatible libraries. These libraries undergo a MiSeq QC run to ensure they are suitable for HiSeq sequencing.

  • Libraries not made at the OGBF are run on the HiSeq at the client’s risk.
  • Libraries must be compatible with the Read 1, Indexing, and Read 2 primers used by the current Illumina chemistry.
  • Dilutions must be based on quantitation by fluorometry or qPCR, and average size and/or primer/adapter dimer contamination assessed by using a Bioanalyzer or equivalent, e.g. Fragment Analyser, TapeStation.
  • For single libraries, please submit >20 uL of library at 10 nM concentration diluted with RSB or EB, i.e. 10 mM Tris pH8.5 (with or without 0.01% Tween-20) in 1.5 mL low-bind tubes.
  • For multiplexed library pools, unless otherwise discussed with us, please submit >20 uL at 10 nM (i.e. do not submit individual unpooled libraries) in 1.5 mL low-bind tubes.

Client QC requirements prior to library submission

  • Bioanalyser trace of the library or pooled libraries.
  • Details of the library preparation that may be relevant to sequencing efficiency and demultiplexing.
  • Quantification must be by using qubit/picogreen or qPCR.
  • Download our sample submission form.
  • Fill in the "User-supplied libraries" section, adding columns if necessary, and email the form to us.
  • Index sequences must be submitted in the correct orientation for sample sheet entry for demultiplexing.